CA2853677C - Methods and materials for enzymatic synthesis of mogroside compounds - Google Patents

Methods and materials for enzymatic synthesis of mogroside compounds Download PDF

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Publication number
CA2853677C
CA2853677C CA2853677A CA2853677A CA2853677C CA 2853677 C CA2853677 C CA 2853677C CA 2853677 A CA2853677 A CA 2853677A CA 2853677 A CA2853677 A CA 2853677A CA 2853677 C CA2853677 C CA 2853677C
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Prior art keywords
polypeptide
mogroside
amino acid
seq
sequence identity
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CA2853677A
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English (en)
French (fr)
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CA2853677A1 (en
Inventor
Yaoquan Liu
Jung Yeop Lee
Monika KHARE
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Danstar Ferment AG
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Evolva AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/20—Preparation of steroids containing heterocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2853677A 2011-11-23 2012-11-19 Methods and materials for enzymatic synthesis of mogroside compounds Active CA2853677C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161563303P 2011-11-23 2011-11-23
US61/563,303 2011-11-23
PCT/IB2012/002857 WO2013076577A1 (en) 2011-11-23 2012-11-19 Methods and materials for enzymatic synthesis of mogroside compounds

Publications (2)

Publication Number Publication Date
CA2853677A1 CA2853677A1 (en) 2013-05-30
CA2853677C true CA2853677C (en) 2019-01-29

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2853677A Active CA2853677C (en) 2011-11-23 2012-11-19 Methods and materials for enzymatic synthesis of mogroside compounds

Country Status (14)

Country Link
US (2) US9920349B2 (enExample)
EP (1) EP2783009B1 (enExample)
JP (1) JP6084983B2 (enExample)
KR (1) KR101791597B1 (enExample)
CN (1) CN103958693B (enExample)
AU (2) AU2012342114B2 (enExample)
BR (1) BR112014012543A2 (enExample)
CA (1) CA2853677C (enExample)
IN (1) IN2014CN03557A (enExample)
MX (1) MX342851B (enExample)
MY (1) MY170686A (enExample)
RU (1) RU2633644C2 (enExample)
SG (1) SG11201401927PA (enExample)
WO (1) WO2013076577A1 (enExample)

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CN105671108A (zh) 2010-06-02 2016-06-15 沃维公司 甜菊糖苷的重组生产
EP2742142B2 (en) 2011-08-08 2020-03-25 Evolva SA Recombinant production of steviol glycosides
EP2783009B1 (en) 2011-11-23 2016-04-13 Evolva SA Methods and materials for enzymatic synthesis of mogroside compounds
TW201402599A (zh) * 2012-05-30 2014-01-16 Suntory Holdings Ltd 甜菊糖苷糖苷轉化酵素及編碼該酵素之基因
EP2929043A1 (en) 2012-12-04 2015-10-14 Evolva SA Methods and materials for biosynthesis of mogroside compounds
US10017804B2 (en) 2013-02-11 2018-07-10 Evolva Sa Efficient production of steviol glycosides in recombinant hosts
MX2017001859A (es) 2014-08-11 2017-04-11 Evolva Sa Produccion de glicosidos de esteviol en hospederos recombinantes.
JP2017528134A (ja) 2014-09-09 2017-09-28 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． 組み換え宿主におけるステビオールグリコシドの生産
IL287789B2 (en) 2014-09-11 2024-05-01 The State Of Israel Ministry Of Agriculture & Rural Development Agricultural Res Organization Aro Vo Mogroside compounds, methods for their preparation, preparations including them and their uses
CN107466320B (zh) 2014-10-01 2021-11-05 埃沃尔瓦公司 用于生物合成罗汉果苷化合物的方法和材料
EP3208342A4 (en) 2014-10-17 2018-07-04 Suntory Holdings Limited Mogrol glycosyltransferase and gene encoding same
US10626430B2 (en) 2015-01-20 2020-04-21 Suntory Holdings Limited Method for preparing mogroside having no β-1,6-glucoside bond
BR112017016338A2 (en) 2015-01-30 2018-03-27 Evolva Sa production of steviol glycosides in recombinant hosts
CA3255837A1 (en) 2015-03-16 2025-10-30 Dsm Ip Assets B.V. Udp-glycosyltransferases
JP2018522569A (ja) 2015-08-07 2018-08-16 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． 組換え宿主内でのステビオールグリコシドの産生
JP6949838B2 (ja) 2015-10-29 2021-10-13 フィルメニッヒ インコーポレイテッドＦｉｒｍｅｎｉｃｈ Ｉｎｃｏｒｐｏｒａｔｅｄ 高甘味度甘味料
CN106924274B (zh) * 2015-12-29 2019-01-04 成都普睿法药物研发有限公司 葫芦烷型四环三萜类化合物抗肺纤维化应用
MY198001A (en) 2016-04-13 2023-07-25 Evolva SA Production of steviol glycoside in recombinant hosts
EP3458598A1 (en) * 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
WO2017198682A1 (en) 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2018016483A1 (ja) * 2016-07-19 2018-01-25 サントリーホールディングス株式会社 モグロールまたはモグロール配糖体の生産方法
US11396669B2 (en) 2016-11-07 2022-07-26 Evolva Sa Production of steviol glycosides in recombinant hosts
CN111108213B (zh) 2017-05-03 2024-12-13 弗门尼舍公司 制备高强度甜味剂的方法
EP3624595A4 (en) 2017-05-19 2021-03-03 The Coca-Cola Company COMPOSITIONS SWEETED WITH SIAMENOSIDE I AND USES THEREOF
CA3064334A1 (en) 2017-06-02 2018-12-06 Givaudan Sa Compositions
EP3638804A1 (en) 2017-06-15 2020-04-22 Evolva SA Production of mogroside compounds in recombinant hosts
CN107189976B (zh) * 2017-07-28 2022-03-11 李华政 促进罗汉果ugt72b21基因表达的方法
US12338476B2 (en) 2018-11-07 2025-06-24 Firmenich Incorporated Methods for making high intensity sweeteners
CN113286523A (zh) * 2018-11-07 2021-08-20 弗门尼舍公司 制备高强度甜味剂的方法
CA3118924A1 (en) 2018-11-09 2020-05-14 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
CN110669094B (zh) * 2019-10-14 2021-12-21 上海交通大学 罗汉果新苷及其制备方法
KR102362135B1 (ko) * 2019-10-25 2022-02-14 고려대학교 산학협력단 테르펜 당쇄 부가 유도체, 이의 제조방법 및 이를 유효성분으로 함유하는 조성물
CN120230785A (zh) * 2020-03-17 2025-07-01 可口可乐公司 新的罗汉果苷生产系统和方法
CA3179775A1 (en) 2020-04-20 2021-10-28 Givaudan Sa Compositions
GB202007984D0 (en) 2020-05-28 2020-07-15 Givaudan Sa Compositions
CN112063647B (zh) * 2020-09-17 2023-05-02 云南农业大学 酿酒酵母重组菌Cuol01的构建方法、酿酒酵母重组菌Cuol02及应用
CN116064712B (zh) * 2022-10-08 2025-05-06 中国药科大学 一种罗汉果醇的生物合成方法
EP4698673A1 (en) 2023-04-20 2026-02-25 DSM IP Assets B.V. Strains and methods for the production of mogrosides

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* Cited by examiner, † Cited by third party
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US6399321B1 (en) * 1999-08-18 2002-06-04 National Research Council Of Canada Methods for screening UDP-glucose:glycoprotein glucosyltransferase (UGGT) activity and nucleic acid encoding for UGGT
EP1510573A1 (en) * 2003-09-01 2005-03-02 Austria Wirtschaftsservice Gesellschaft mit beschränkter Haftung Method for detoxification of mycotoxins
US20080148432A1 (en) * 2005-12-21 2008-06-19 Mark Scott Abad Transgenic plants with enhanced agronomic traits
WO2006092449A2 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
US8257948B1 (en) * 2011-02-17 2012-09-04 Purecircle Usa Method of preparing alpha-glucosyl Stevia composition
US8956677B2 (en) 2005-11-23 2015-02-17 The Coca-Cola Company High-potency sweetener composition with glucosamine and compositions sweetened therewith
RU2425590C2 (ru) * 2005-11-23 2011-08-10 Дзе Кока-Кола Компани Интенсивный подсластитель для гидратации и подслащенная гидратирующая композиция
US7927851B2 (en) 2006-03-21 2011-04-19 Vineland Research And Innovation Centre Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same
WO2008062165A2 (en) * 2006-11-22 2008-05-29 The University Of York Monoterpenoid modifying enzymes
US20100132073A1 (en) * 2006-12-01 2010-05-27 The University Of York Sesquiterpenoid modifying enzymes
GB0904639D0 (en) * 2009-03-18 2009-04-29 Univ York Assay
CN105671108A (zh) 2010-06-02 2016-06-15 沃维公司 甜菊糖苷的重组生产
EP2783009B1 (en) 2011-11-23 2016-04-13 Evolva SA Methods and materials for enzymatic synthesis of mogroside compounds
EP2929043A1 (en) 2012-12-04 2015-10-14 Evolva SA Methods and materials for biosynthesis of mogroside compounds

Also Published As

Publication number Publication date
RU2014125216A (ru) 2015-12-27
JP6084983B2 (ja) 2017-02-22
US9920349B2 (en) 2018-03-20
JP2014533518A (ja) 2014-12-15
AU2017235907A1 (en) 2017-10-12
MX342851B (es) 2016-10-14
HK1200494A1 (zh) 2015-08-07
KR20140099281A (ko) 2014-08-11
KR101791597B1 (ko) 2017-10-30
EP2783009B1 (en) 2016-04-13
IN2014CN03557A (enExample) 2015-10-09
RU2633644C2 (ru) 2017-10-16
SG11201401927PA (en) 2014-09-26
US10738340B2 (en) 2020-08-11
WO2013076577A1 (en) 2013-05-30
US20140308698A1 (en) 2014-10-16
BR112014012543A2 (pt) 2017-06-13
EP2783009A1 (en) 2014-10-01
MY170686A (en) 2019-08-26
CN103958693A (zh) 2014-07-30
AU2012342114A1 (en) 2014-05-22
CN103958693B (zh) 2016-08-24
CA2853677A1 (en) 2013-05-30
AU2012342114B2 (en) 2017-12-21
US20180237819A1 (en) 2018-08-23
MX2014006276A (es) 2014-10-13

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