MX342851B - Metodos y materiales para sintesis enzimatica de compuestos mogrosido. - Google Patents

Metodos y materiales para sintesis enzimatica de compuestos mogrosido.

Info

Publication number
MX342851B
MX342851B MX2014006276A MX2014006276A MX342851B MX 342851 B MX342851 B MX 342851B MX 2014006276 A MX2014006276 A MX 2014006276A MX 2014006276 A MX2014006276 A MX 2014006276A MX 342851 B MX342851 B MX 342851B
Authority
MX
Mexico
Prior art keywords
materials
methods
enzymatic synthesis
mogroside compounds
mogroside
Prior art date
Application number
MX2014006276A
Other languages
English (en)
Other versions
MX2014006276A (es
Inventor
Liu Yaoquan
Yeop Lee Jung
Khare Monika
Original Assignee
Evolva Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Sa filed Critical Evolva Sa
Publication of MX2014006276A publication Critical patent/MX2014006276A/es
Publication of MX342851B publication Critical patent/MX342851B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/20—Preparation of steroids containing heterocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención se refiere a un método para producir un compuesto mogrosido, que comprende poner en contacto y glicosilar un mogrol con un polipéptido de Uridina-5´difosfo (UDP) dependiente de glucosiltransferasa ( UGT) que es: (a) polipéptido UGT3C3 que tiene al menos 90% de identidad de secuencia con la secuencia de aminoácidos expuesta en SEQ ID NO: 1; (b) polipéptido UGT73C5 que tiene al menos 90% de identidad de secuencia con la secuencia de aminoácidos expuesta en SEQ ID NO: 2; (c) polipéptido UGT3C6 que tiene al menos 90% de identidad de secuencia con la secuencia de aminoácidos expuesta en SEQ ID NO: 3; (d) polipéptido UGT73E1 que tiene al menos 90% de identidad de secuencia con la secuencia de aminoácidos expuesta en SEQ ID NO: 4; o (e) polipéptido UGT85C2 que tiene al menos 90% de identidad de secuencia con la secuencia de aminoácidos expuesta en SEQ ID NO: 5.
MX2014006276A 2011-11-23 2012-11-19 Metodos y materiales para sintesis enzimatica de compuestos mogrosido. MX342851B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161563303P 2011-11-23 2011-11-23
PCT/IB2012/002857 WO2013076577A1 (en) 2011-11-23 2012-11-19 Methods and materials for enzymatic synthesis of mogroside compounds

Publications (2)

Publication Number Publication Date
MX2014006276A MX2014006276A (es) 2014-10-13
MX342851B true MX342851B (es) 2016-10-14

Family

ID=47624376

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014006276A MX342851B (es) 2011-11-23 2012-11-19 Metodos y materiales para sintesis enzimatica de compuestos mogrosido.

Country Status (15)

Country Link
US (2) US9920349B2 (es)
EP (1) EP2783009B1 (es)
JP (1) JP6084983B2 (es)
KR (1) KR101791597B1 (es)
CN (1) CN103958693B (es)
AU (2) AU2012342114B2 (es)
BR (1) BR112014012543A2 (es)
CA (1) CA2853677C (es)
HK (1) HK1200494A1 (es)
IN (1) IN2014CN03557A (es)
MX (1) MX342851B (es)
MY (1) MY170686A (es)
RU (1) RU2633644C2 (es)
SG (1) SG11201401927PA (es)
WO (1) WO2013076577A1 (es)

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NZ708078A (en) 2010-06-02 2017-01-27 Evolva Nutrition Inc Recombinant production of steviol glycosides
BR112014003037B1 (pt) 2011-08-08 2022-04-05 Evolva Sa Hospedeiro recombinante e método para produzir um glicosídeo de esteviol
KR101791597B1 (ko) 2011-11-23 2017-10-30 에볼바 에스아 모그로사이드 화합물을 효소에 의해 합성하기 위한 방법 및 재료
TW201402599A (zh) * 2012-05-30 2014-01-16 Suntory Holdings Ltd 甜菊糖苷糖苷轉化酵素及編碼該酵素之基因
WO2014086842A1 (en) 2012-12-04 2014-06-12 Evolva Sa Methods and materials for biosynthesis of mogroside compounds
BR112015019160A2 (pt) 2013-02-11 2017-08-22 Dalgaard Mikkelsen Michael Produção de glicosídeos de esteviol em hospedeiros recombinantes
SG11201700651RA (en) 2014-08-11 2017-02-27 Evolva Sa Production of steviol glycosides in recombinant hosts
CN107109358B (zh) 2014-09-09 2022-08-02 埃沃尔瓦公司 在重组宿主中生产甜菊醇糖苷
IL287789B2 (en) * 2014-09-11 2024-05-01 The State Of Israel Ministry Of Agriculture & Rural Development Agricultural Res Organization Aro Vo Mogroside compounds, methods for their preparation, preparations including them and their uses
CN107466320B (zh) 2014-10-01 2021-11-05 埃沃尔瓦公司 用于生物合成罗汉果苷化合物的方法和材料
CA2968331C (en) 2014-10-17 2024-02-06 Suntory Holdings Limited Mogrol glycosyltransferase and gene encoding same
CA2974185A1 (en) 2015-01-20 2016-07-28 Suntory Holdings Limited Method for preparing mogroside
WO2016120486A1 (en) 2015-01-30 2016-08-04 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2016146711A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
AU2016307066A1 (en) 2015-08-07 2018-02-08 Evolva Sa Production of steviol glycosides in recombinant hosts
MY190296A (en) 2015-10-29 2022-04-12 Firmenich Incorporated High intensity sweeteners
CN106924274B (zh) * 2015-12-29 2019-01-04 成都普睿法药物研发有限公司 葫芦烷型四环三萜类化合物抗肺纤维化应用
WO2017178632A1 (en) 2016-04-13 2017-10-19 Evolva Sa Production of steviol glycosides in recombinant hosts
SG11201809483UA (en) * 2016-05-16 2018-11-29 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3458599A1 (en) 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
CN109477126B (zh) * 2016-07-19 2023-06-20 三得利控股株式会社 罗汉果醇或罗汉果醇糖苷的生产方法
US11396669B2 (en) 2016-11-07 2022-07-26 Evolva Sa Production of steviol glycosides in recombinant hosts
BR112019023104A2 (pt) 2017-05-03 2020-11-03 Firmenich Incorporated métodos para fazer adoçante de alta intensidade
JP7285788B2 (ja) 2017-05-19 2023-06-02 ザ コカ・コーラ カンパニー シアメノシドｉ甘味料入り組成物及びその使用
MX2019014119A (es) 2017-06-02 2020-02-07 Givaudan Sa Composiciones.
WO2021214008A1 (en) 2020-04-20 2021-10-28 Givaudan Sa Compositions
WO2018229283A1 (en) 2017-06-15 2018-12-20 Evolva Sa Production of mogroside compounds in recombinant hosts
CN107189976B (zh) * 2017-07-28 2022-03-11 李华政 促进罗汉果ugt72b21基因表达的方法
CN110669094B (zh) * 2019-10-14 2021-12-21 上海交通大学 罗汉果新苷及其制备方法
KR102362135B1 (ko) * 2019-10-25 2022-02-14 고려대학교 산학협력단 테르펜 당쇄 부가 유도체, 이의 제조방법 및 이를 유효성분으로 함유하는 조성물
EP4120823A4 (en) * 2020-03-17 2024-08-07 Coca Cola Co INNOVATIVE SYSTEM AND PROCESSES FOR PRODUCTION OF MOGROSIDE
GB202007984D0 (en) 2020-05-28 2020-07-15 Givaudan Sa Compositions
CN112063647B (zh) * 2020-09-17 2023-05-02 云南农业大学 酿酒酵母重组菌Cuol01的构建方法、酿酒酵母重组菌Cuol02及应用

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US6399321B1 (en) * 1999-08-18 2002-06-04 National Research Council Of Canada Methods for screening UDP-glucose:glycoprotein glucosyltransferase (UGGT) activity and nucleic acid encoding for UGGT
EP1510573A1 (en) 2003-09-01 2005-03-02 Austria Wirtschaftsservice Gesellschaft mit beschränkter Haftung Method for detoxification of mycotoxins
US20080148432A1 (en) * 2005-12-21 2008-06-19 Mark Scott Abad Transgenic plants with enhanced agronomic traits
CA2598792A1 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
US8257948B1 (en) * 2011-02-17 2012-09-04 Purecircle Usa Method of preparing alpha-glucosyl Stevia composition
US8956677B2 (en) 2005-11-23 2015-02-17 The Coca-Cola Company High-potency sweetener composition with glucosamine and compositions sweetened therewith
RU2417031C2 (ru) * 2005-11-23 2011-04-27 Дзе Кока-Кола Компани Композиция интенсивного подсластителя с минеральным веществом и подслащенные ею композиции
US7927851B2 (en) 2006-03-21 2011-04-19 Vineland Research And Innovation Centre Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same
US20100143975A1 (en) * 2006-11-22 2010-06-10 The University Of York Monoterpenoid modifying enzymes
US20100132073A1 (en) * 2006-12-01 2010-05-27 The University Of York Sesquiterpenoid modifying enzymes
GB0904639D0 (en) * 2009-03-18 2009-04-29 Univ York Assay
NZ708078A (en) 2010-06-02 2017-01-27 Evolva Nutrition Inc Recombinant production of steviol glycosides
KR101791597B1 (ko) 2011-11-23 2017-10-30 에볼바 에스아 모그로사이드 화합물을 효소에 의해 합성하기 위한 방법 및 재료
WO2014086842A1 (en) 2012-12-04 2014-06-12 Evolva Sa Methods and materials for biosynthesis of mogroside compounds

Also Published As

Publication number Publication date
AU2012342114A1 (en) 2014-05-22
WO2013076577A1 (en) 2013-05-30
CA2853677A1 (en) 2013-05-30
SG11201401927PA (en) 2014-09-26
US20140308698A1 (en) 2014-10-16
IN2014CN03557A (es) 2015-10-09
MY170686A (en) 2019-08-26
US10738340B2 (en) 2020-08-11
JP2014533518A (ja) 2014-12-15
AU2012342114B2 (en) 2017-12-21
KR20140099281A (ko) 2014-08-11
KR101791597B1 (ko) 2017-10-30
RU2633644C2 (ru) 2017-10-16
CN103958693B (zh) 2016-08-24
BR112014012543A2 (pt) 2017-06-13
US9920349B2 (en) 2018-03-20
AU2017235907A1 (en) 2017-10-12
CN103958693A (zh) 2014-07-30
HK1200494A1 (zh) 2015-08-07
JP6084983B2 (ja) 2017-02-22
MX2014006276A (es) 2014-10-13
RU2014125216A (ru) 2015-12-27
US20180237819A1 (en) 2018-08-23
EP2783009B1 (en) 2016-04-13
EP2783009A1 (en) 2014-10-01
CA2853677C (en) 2019-01-29

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