CN103958693B - 用于酶促合成罗汉果苷化合物的方法和材料 - Google Patents

用于酶促合成罗汉果苷化合物的方法和材料 Download PDF

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Publication number
CN103958693B
CN103958693B CN201280057518.8A CN201280057518A CN103958693B CN 103958693 B CN103958693 B CN 103958693B CN 201280057518 A CN201280057518 A CN 201280057518A CN 103958693 B CN103958693 B CN 103958693B
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mogroside
polypeptide
ugt
seq
contacting
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CN103958693A (zh
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刘耀权
李正烨
莫妮卡·哈雷
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Danstar Ferment AG
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Evolva AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/20—Preparation of steroids containing heterocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201280057518.8A 2011-11-23 2012-11-19 用于酶促合成罗汉果苷化合物的方法和材料 Active CN103958693B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161563303P 2011-11-23 2011-11-23
US61/563,303 2011-11-23
PCT/IB2012/002857 WO2013076577A1 (en) 2011-11-23 2012-11-19 Methods and materials for enzymatic synthesis of mogroside compounds

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CN103958693A CN103958693A (zh) 2014-07-30
CN103958693B true CN103958693B (zh) 2016-08-24

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US (2) US9920349B2 (enExample)
EP (1) EP2783009B1 (enExample)
JP (1) JP6084983B2 (enExample)
KR (1) KR101791597B1 (enExample)
CN (1) CN103958693B (enExample)
AU (2) AU2012342114B2 (enExample)
BR (1) BR112014012543A2 (enExample)
CA (1) CA2853677C (enExample)
IN (1) IN2014CN03557A (enExample)
MX (1) MX342851B (enExample)
MY (1) MY170686A (enExample)
RU (1) RU2633644C2 (enExample)
SG (1) SG11201401927PA (enExample)
WO (1) WO2013076577A1 (enExample)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR122021005297B1 (pt) 2010-06-02 2022-02-22 Evolva, Inc Método para transferir uma segunda porção de açúcar para uma posição c-2' de uma glicose em um glicosídeo de esteviol
JP6262653B2 (ja) 2011-08-08 2018-01-17 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． ステビオール配糖体の組換え生産
AU2012342114B2 (en) 2011-11-23 2017-12-21 Danstar Ferment Ag Methods and materials for enzymatic synthesis of mogroside compounds
TW201402599A (zh) * 2012-05-30 2014-01-16 Suntory Holdings Ltd 甜菊糖苷糖苷轉化酵素及編碼該酵素之基因
WO2016050890A2 (en) 2014-10-01 2016-04-07 Evolva Sa Methods and materials for biosynthesis of mogroside compounds
US9932619B2 (en) * 2012-12-04 2018-04-03 Evolva Sa Methods and materials for biosynthesis of mogroside compounds
CN105189771B (zh) 2013-02-11 2019-11-05 埃沃尔瓦公司 在重组宿主中有效产生甜菊醇糖苷
CA2957331A1 (en) 2014-08-11 2016-02-18 Evolva Sa Production of steviol glycosides in recombinant hosts
US10612064B2 (en) 2014-09-09 2020-04-07 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3191584A4 (en) 2014-09-11 2018-07-18 The State of Israel - Ministry of Agriculture & Rural Development, Agricultural Research Organization (ARO) (Volcani Center) Methods of producing mogrosides and compositions comprising same and uses thereof
EP3208342A4 (en) * 2014-10-17 2018-07-04 Suntory Holdings Limited Mogrol glycosyltransferase and gene encoding same
CA2974185A1 (en) 2015-01-20 2016-07-28 Suntory Holdings Limited Method for preparing mogroside
EP4148137A1 (en) 2015-01-30 2023-03-15 Evolva SA Production of steviol glycosides in recombinant hosts
CA2979931A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
BR112018002564A2 (pt) 2015-08-07 2018-09-25 Evolva Sa produção de glicosídeos de esteviol em hospedeiros recombinantes
JP6949838B2 (ja) 2015-10-29 2021-10-13 フィルメニッヒ インコーポレイテッドＦｉｒｍｅｎｉｃｈ Ｉｎｃｏｒｐｏｒａｔｅｄ 高甘味度甘味料
CN106924274B (zh) * 2015-12-29 2019-01-04 成都普睿法药物研发有限公司 葫芦烷型四环三萜类化合物抗肺纤维化应用
CN109195457A (zh) 2016-04-13 2019-01-11 埃沃尔瓦公司 在重组宿主中产生甜菊醇糖苷
US10815514B2 (en) 2016-05-16 2020-10-27 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2017198681A1 (en) * 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
CN109477126B (zh) 2016-07-19 2023-06-20 三得利控股株式会社 罗汉果醇或罗汉果醇糖苷的生产方法
CN110100006A (zh) 2016-11-07 2019-08-06 埃沃尔瓦公司 重组宿主中甜菊糖苷的生产
JP7285786B2 (ja) 2017-05-03 2023-06-02 フィルメニッヒ インコーポレイテッド 高強度の甘味料の製造方法
JP7285788B2 (ja) 2017-05-19 2023-06-02 ザ コカ・コーラ カンパニー シアメノシドｉ甘味料入り組成物及びその使用
CN116941752A (zh) 2017-06-02 2023-10-27 奇华顿股份有限公司 组合物
WO2018229283A1 (en) 2017-06-15 2018-12-20 Evolva Sa Production of mogroside compounds in recombinant hosts
CN107189976B (zh) * 2017-07-28 2022-03-11 李华政 促进罗汉果ugt72b21基因表达的方法
JP2022512931A (ja) 2018-11-07 2022-02-07 フィルメニッヒ インコーポレイテッド 高甘味度甘味料の製造方法
US12286661B2 (en) 2018-11-07 2025-04-29 Firmenich Incorporated Methods for making high intensity sweeteners
US12234464B2 (en) 2018-11-09 2025-02-25 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
CN110669094B (zh) * 2019-10-14 2021-12-21 上海交通大学 罗汉果新苷及其制备方法
KR102362135B1 (ko) * 2019-10-25 2022-02-14 고려대학교 산학협력단 테르펜 당쇄 부가 유도체, 이의 제조방법 및 이를 유효성분으로 함유하는 조성물
MX2022011627A (es) * 2020-03-17 2022-12-15 Coca Cola Co Nuevo sistema y métodos de producción de mogrósidos.
CA3179775A1 (en) 2020-04-20 2021-10-28 Givaudan Sa Compositions
GB202007984D0 (en) 2020-05-28 2020-07-15 Givaudan Sa Compositions
CN112063647B (zh) * 2020-09-17 2023-05-02 云南农业大学 酿酒酵母重组菌Cuol01的构建方法、酿酒酵母重组菌Cuol02及应用
CN116064712B (zh) * 2022-10-08 2025-05-06 中国药科大学 一种罗汉果醇的生物合成方法
CN121127603A (zh) 2023-04-20 2025-12-12 帝斯曼知识产权资产管理有限公司 用于产生罗汉果苷的菌株和方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008062165A2 (en) * 2006-11-22 2008-05-29 The University Of York Monoterpenoid modifying enzymes
WO2008065370A2 (en) * 2006-12-01 2008-06-05 The University Of York Sesquiterpenoid modifying enzymes
WO2010106318A1 (en) * 2009-03-18 2010-09-23 The University Of York Screening plant glycosyltransferases for glycosylation of terpenoids in planta

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6399321B1 (en) * 1999-08-18 2002-06-04 National Research Council Of Canada Methods for screening UDP-glucose:glycoprotein glucosyltransferase (UGGT) activity and nucleic acid encoding for UGGT
EP1510573A1 (en) * 2003-09-01 2005-03-02 Austria Wirtschaftsservice Gesellschaft mit beschränkter Haftung Method for detoxification of mycotoxins
US20080148432A1 (en) * 2005-12-21 2008-06-19 Mark Scott Abad Transgenic plants with enhanced agronomic traits
AU2006219823A1 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
US8257948B1 (en) * 2011-02-17 2012-09-04 Purecircle Usa Method of preparing alpha-glucosyl Stevia composition
US8956677B2 (en) 2005-11-23 2015-02-17 The Coca-Cola Company High-potency sweetener composition with glucosamine and compositions sweetened therewith
RU2444900C2 (ru) * 2005-11-23 2012-03-20 Дзе Кока-Кола Компани Молочная композиция с интенсивным подсластителем
US7927851B2 (en) 2006-03-21 2011-04-19 Vineland Research And Innovation Centre Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same
BR122021005297B1 (pt) 2010-06-02 2022-02-22 Evolva, Inc Método para transferir uma segunda porção de açúcar para uma posição c-2' de uma glicose em um glicosídeo de esteviol
AU2012342114B2 (en) 2011-11-23 2017-12-21 Danstar Ferment Ag Methods and materials for enzymatic synthesis of mogroside compounds
US9932619B2 (en) 2012-12-04 2018-04-03 Evolva Sa Methods and materials for biosynthesis of mogroside compounds

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008062165A2 (en) * 2006-11-22 2008-05-29 The University Of York Monoterpenoid modifying enzymes
WO2008065370A2 (en) * 2006-12-01 2008-06-05 The University Of York Sesquiterpenoid modifying enzymes
WO2010106318A1 (en) * 2009-03-18 2010-09-23 The University Of York Screening plant glycosyltransferases for glycosylation of terpenoids in planta

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Glycosyltransferases: managers of small molecules;Dianna Bowles等;《Current Opinion in Plant Biology》;20050630;第8卷(第3期);表1 *
罗汉果三萜皂苷生物合成规律研究探究;熊绵靖等;《广东药学院学报》;20111031;第27卷(第5期);第545页第1段、图1 *

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Publication number Publication date
US20140308698A1 (en) 2014-10-16
US20180237819A1 (en) 2018-08-23
CA2853677A1 (en) 2013-05-30
EP2783009A1 (en) 2014-10-01
JP6084983B2 (ja) 2017-02-22
CA2853677C (en) 2019-01-29
KR101791597B1 (ko) 2017-10-30
AU2017235907A1 (en) 2017-10-12
MX2014006276A (es) 2014-10-13
EP2783009B1 (en) 2016-04-13
BR112014012543A2 (pt) 2017-06-13
CN103958693A (zh) 2014-07-30
KR20140099281A (ko) 2014-08-11
SG11201401927PA (en) 2014-09-26
WO2013076577A1 (en) 2013-05-30
RU2014125216A (ru) 2015-12-27
US9920349B2 (en) 2018-03-20
MY170686A (en) 2019-08-26
AU2012342114B2 (en) 2017-12-21
RU2633644C2 (ru) 2017-10-16
HK1200494A1 (zh) 2015-08-07
JP2014533518A (ja) 2014-12-15
US10738340B2 (en) 2020-08-11
MX342851B (es) 2016-10-14
AU2012342114A1 (en) 2014-05-22
IN2014CN03557A (enExample) 2015-10-09

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Effective date of registration: 20250910

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Patentee before: EVOLVA S.A.

Country or region before: Switzerland