CN113444703B - 催化糖链延伸的糖基转移酶突变体及其应用 - Google Patents
催化糖链延伸的糖基转移酶突变体及其应用 Download PDFInfo
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- CN113444703B CN113444703B CN202010221972.9A CN202010221972A CN113444703B CN 113444703 B CN113444703 B CN 113444703B CN 202010221972 A CN202010221972 A CN 202010221972A CN 113444703 B CN113444703 B CN 113444703B
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- ginsenoside
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P33/00—Preparation of steroids
- C12P33/20—Preparation of steroids containing heterocyclic rings
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/01—Hydro-lyases (4.2.1)
- C12Y402/01125—Dammarenediol II synthase (4.2.1.125)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
底物 | R1 | R2 | R3 | R4 | 产物 |
Rg1 | H | Glc | Glc | Rha | 人参皂苷Re |
Rh1 | H | H | Glc | Rha | 人参皂苷Rg2 |
底物 | R1 | R2 | R3 | R4 | R5 | 产物 |
人参皂苷Re4 | H | Glc | Ara(f) | Glc | Rha | Floralgensenoside M |
三七皂苷R3 | H | Glc | Glc | Glc | Rha | Yesanchinoside E |
三七皂苷Fp1 | H | Glc | Ara(p) | Glc | Rha | Floralgensenoside N |
西洋参皂苷L17 | H | Glc | Xyl | Glc | Rha | Floralquinquenoside E |
标签 | 残基数 | 序列 |
Poly-Arg | 5-6个(通常5个) | RRRRR |
Poly-His | 2-10个(通常6个) | HHHHHH |
FLAG | 8个 | DYKDDDDK |
Strep-TagII | 8个 | WSHPQFEK |
C-myc | 10个 | WQKLISEEDL |
GST | 220个 | 后面6个LVPRGS |
Claims (29)
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CN202010221972.9A CN113444703B (zh) | 2020-03-26 | 2020-03-26 | 催化糖链延伸的糖基转移酶突变体及其应用 |
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CN113444703B true CN113444703B (zh) | 2023-09-01 |
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Citations (6)
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CN103849672A (zh) * | 2012-12-06 | 2014-06-11 | 中国科学院上海生命科学研究院 | 一组糖基转移酶及其应用 |
EP2821482A1 (en) * | 2013-07-05 | 2015-01-07 | Roche Diagniostics GmbH | N-terminally truncated glycosyltransferases |
CN106459987A (zh) * | 2014-04-30 | 2017-02-22 | 韩国科学技术院 | 使用源自于人参的糖基转移酶使人参皂苷糖基化的新方法 |
CN108866020A (zh) * | 2017-05-16 | 2018-11-23 | 中国科学院上海生命科学研究院 | 糖基转移酶、突变体及其应用 |
CN108949711A (zh) * | 2017-05-19 | 2018-12-07 | 中国科学院上海生命科学研究院 | 一组催化糖链延伸的udp-糖基转移酶及其应用 |
CN109796516A (zh) * | 2017-11-17 | 2019-05-24 | 中国科学院天津工业生物技术研究所 | 一组天然与非天然的原人参三醇型人参皂苷的合成方法 |
-
2020
- 2020-03-26 CN CN202010221972.9A patent/CN113444703B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
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