KR100808517B1 - α-아밀라제 변이체 - Google Patents
α-아밀라제 변이체 Download PDFInfo
- Publication number
- KR100808517B1 KR100808517B1 KR1020017012076A KR20017012076A KR100808517B1 KR 100808517 B1 KR100808517 B1 KR 100808517B1 KR 1020017012076 A KR1020017012076 A KR 1020017012076A KR 20017012076 A KR20017012076 A KR 20017012076A KR 100808517 B1 KR100808517 B1 KR 100808517B1
- Authority
- KR
- South Korea
- Prior art keywords
- gly
- amylase
- seq
- asp
- val
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Detergent Compositions (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA199900437 | 1999-03-30 | ||
| DKPA199900437 | 1999-03-30 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20010108369A KR20010108369A (ko) | 2001-12-07 |
| KR100808517B1 true KR100808517B1 (ko) | 2008-02-29 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020017012076A Expired - Lifetime KR100808517B1 (ko) | 1999-03-30 | 2000-03-28 | α-아밀라제 변이체 |
Country Status (15)
| Country | Link |
|---|---|
| EP (3) | EP2290060B1 (https=) |
| JP (1) | JP4668426B2 (https=) |
| KR (1) | KR100808517B1 (https=) |
| CN (1) | CN1252256C (https=) |
| AR (1) | AR023214A1 (https=) |
| AT (1) | ATE360686T1 (https=) |
| AU (1) | AU3419300A (https=) |
| BR (1) | BRPI0009362B8 (https=) |
| CA (1) | CA2365438C (https=) |
| DE (1) | DE60034558T2 (https=) |
| DK (3) | DK2290060T3 (https=) |
| ES (3) | ES2286012T3 (https=) |
| MX (1) | MXPA01009812A (https=) |
| RU (1) | RU2231547C2 (https=) |
| WO (1) | WO2000060059A2 (https=) |
Families Citing this family (201)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2417547A1 (en) | 2000-07-28 | 2003-01-28 | Henkel Kommanditgesellschaft Auf Aktien | Novel amylolytic enzyme extracted from bacillus sp. a 7-7 (dsm 12368) and washing and cleaning agents containing this novel amylolytic enzyme |
| EP1370648A2 (en) * | 2000-08-01 | 2003-12-17 | Novozymes A/S | Alpha-amylase mutants with altered properties |
| US20020155574A1 (en) | 2000-08-01 | 2002-10-24 | Novozymes A/S | Alpha-amylase mutants with altered properties |
| EP1326965A2 (en) * | 2000-10-13 | 2003-07-16 | Novozymes A/S | Alpha-amylase variant with altered properties |
| EP1975229A3 (en) * | 2000-10-13 | 2009-03-18 | Novozymes A/S | Alpha-amylase variant with altered properties |
| CA3081363A1 (en) * | 2001-02-21 | 2002-09-06 | Basf Enzymes Llc | Enzymes having alpha amylase activity and methods of use thereof |
| EP2159279A3 (en) | 2001-05-15 | 2010-05-12 | Novozymes A/S | Alpha-amylase variant with altered properties |
| DE10138753B4 (de) * | 2001-08-07 | 2017-07-20 | Henkel Ag & Co. Kgaa | Wasch- und Reinigungsmittel mit Hybrid-Alpha-Amylasen |
| US7579177B2 (en) | 2003-05-30 | 2009-08-25 | Novozymes A/S | Alcohol product processes |
| CN104357424A (zh) * | 2004-07-07 | 2015-02-18 | 杜邦营养生物科学有限公司 | 多肽 |
| EP2258837A1 (en) * | 2004-09-10 | 2010-12-08 | Novozymes North America, Inc. | Methods for preventing, removing, reducing, or disrupting biofilm |
| DE102004047776B4 (de) | 2004-10-01 | 2018-05-09 | Basf Se | Gegen Di- und/oder Multimerisierung stabilisierte Alpha-Amylase-Varianten, Verfahren zu deren Herstellung sowie deren Verwendung |
| DE102004047777B4 (de) | 2004-10-01 | 2018-05-09 | Basf Se | Alpha-Amylase-Varianten mit erhöhter Lösungsmittelstabilität, Verfahren zu deren Herstellung sowie deren Verwendung |
| EP2365068B1 (en) | 2004-12-22 | 2017-03-01 | Novozymes A/S | Enzymes for starch processing |
| EP1926753B1 (en) * | 2004-12-22 | 2010-11-24 | Novozymes A/S | Hybrid enzymes consisting of an endo-amylase first amino acid sequence and a carbohydrate-binding module as second amino acid sequence |
| CN101268195A (zh) | 2005-09-20 | 2008-09-17 | 诺维信北美公司 | 产生发酵产物的方法 |
| WO2007076388A2 (en) | 2005-12-22 | 2007-07-05 | Novozymes North America, Inc. | Processes for producing a fermentation product |
| CN101405397A (zh) | 2006-03-22 | 2009-04-08 | 诺维信北美公司 | 发酵方法 |
| RU2426775C2 (ru) * | 2006-04-04 | 2011-08-20 | Новозимс А/С | Способ получения сусла |
| KR20090101193A (ko) * | 2006-12-21 | 2009-09-24 | 다니스코 유에스 인크. | 바실러스 종 １９５ 의 알파-아밀라아제 폴리펩티드에 대한 조성물 및 용도 |
| RU2009139059A (ru) | 2007-03-23 | 2011-04-27 | ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН (US) | Улучшенное производство амилазы посредством присоединения к n-концу зрелого белка амилазы |
| ES2748245T3 (es) | 2007-04-24 | 2020-03-16 | Novozymes North America Inc | Detoxificación de materiales que contienen lignocelulosa pretratados |
| WO2009030713A1 (en) | 2007-09-03 | 2009-03-12 | Novozymes A/S | Detoxifying and recycling of washing solution used in pretreatment of lignocellulose-containing materials |
| EP2215201A2 (en) * | 2007-11-05 | 2010-08-11 | Danisco US Inc. | Alpha-amylase variants with altered properties |
| CN102057040A (zh) * | 2008-06-06 | 2011-05-11 | 丹尼斯科美国公司 | 具有改良特性的嗜热脂肪土芽孢杆菌α-淀粉酶(AMYS)变体 |
| US20110097779A1 (en) | 2008-06-23 | 2011-04-28 | Chee-Leong Soong | Processes for Producing Fermentation Products |
| BRPI0919525A2 (pt) | 2008-09-30 | 2015-08-18 | Novozymes North America Inc | Métodos para produzir um produto de fermentação a partir de um material contendo lignocelulose, para intensificar hidrólise enzimática e para reduzir o efeito prejudicial de lignina na hidrólise de um material contendo lignocelulose, e, produto de fermentação |
| CN102292444A (zh) | 2008-11-20 | 2011-12-21 | 诺维信股份有限公司 | 具有淀粉分解增强活性的多肽和编码该多肽的多核苷酸 |
| DK2358873T4 (en) * | 2008-12-15 | 2018-05-28 | Danisco Us Inc | HYBRID ALPHA AMYLASER |
| US20120028299A1 (en) | 2008-12-30 | 2012-02-02 | Novozymes North America, Inc. | Enzymatic Hydrolysis Of Pretreated Lignocellulose-Containing Material With Dissolved Air Flotation Sludge |
| WO2010078392A2 (en) | 2008-12-31 | 2010-07-08 | Novozymes North America, Inc. | Processes of producing fermentation products |
| CN102498401B (zh) | 2009-07-17 | 2015-12-02 | 诺维信公司 | 在纤维素材料水解中分析纤维素衰变的方法 |
| WO2011066560A1 (en) | 2009-11-30 | 2011-06-03 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| MX2012006096A (es) | 2009-11-30 | 2012-07-03 | Novozymes North America Inc | Polipeptidos que tienen actividad glucoamilasa y polinucleotidos que codifican para los mismos. |
| ES2534067T3 (es) | 2009-12-01 | 2015-04-17 | Novozymes A/S | Polipéptidos que tienen actividad de glucoamilasa y polinucleótidos que codifican los mismos |
| CN102933227A (zh) | 2009-12-22 | 2013-02-13 | 诺维信公司 | 包含增强性多肽和淀粉降解酶的组合物及其用途 |
| US20120270267A1 (en) * | 2010-01-04 | 2012-10-25 | Novozymes A/S | Alpha-Amylases |
| US20130040354A1 (en) | 2010-01-29 | 2013-02-14 | Novozymes A/S | Biogas Production Process With Enzymatic Pre-Treatment |
| WO2011100161A1 (en) | 2010-02-09 | 2011-08-18 | Novozymes North America, Inc. | Addition of alpha - glucosidase and cobalt for producing fermentation products from starch |
| US9896673B2 (en) * | 2010-02-10 | 2018-02-20 | Novozymes A/S | Compositions of high stability alpha amylase variants |
| EP2553111B1 (en) | 2010-03-30 | 2016-05-11 | Novozymes A/S | Methods for enhancing by-products from fermentation processes |
| MX338068B (es) | 2010-04-14 | 2016-04-01 | Novozymes As | Polipeptidos que tienen actividad de glucoamilasa y polinucleotidos que codifican los mismos. |
| US20130157307A1 (en) | 2010-08-02 | 2013-06-20 | Novozymes North America, Inc. | Process of Producing A Fermentation Product |
| CN103298932B (zh) | 2010-11-08 | 2016-08-10 | 诺维信公司 | 具有葡糖淀粉酶活性的多肽和编码该多肽的多核苷酸 |
| EP2637515B1 (en) | 2010-11-08 | 2017-09-06 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| US9057087B2 (en) | 2010-11-19 | 2015-06-16 | Novozymes North America, Inc. | Processes of producing a fermentation product |
| EP2661499A1 (en) | 2011-01-04 | 2013-11-13 | Novozymes A/S | Process for producing biogas from pectin and lignocellulose containing material |
| US9677094B2 (en) | 2011-02-07 | 2017-06-13 | Novozymes A/S | Process of producing a fermentation product |
| MX2013014236A (es) | 2011-06-28 | 2014-01-23 | Novozymes As | Biogas a partir de bagazo tratado con enzimas. |
| ES2834102T3 (es) | 2011-06-30 | 2021-06-16 | Novozymes As | Variantes de alfa-amilasa |
| BR112014000143B1 (pt) | 2011-07-06 | 2021-04-06 | Novozymes A/S | Variante de alfa-amilase, polinucleotídeo isolado, vetor de expressão, célula hospedeira, métodos de produzir uma variante de alfa-amilase, para produzir um produto de fermentação a partir de um material contendo amido, e, para a produção um derivado de amido enzimaticamente modificado |
| EP2734633B1 (en) | 2011-07-22 | 2019-05-01 | Novozymes North America, Inc. | Processes for pretreating cellulosic material and improving hydrolysis thereof |
| CA2846690A1 (en) | 2011-08-26 | 2013-03-07 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| EA201490568A1 (ru) | 2011-09-06 | 2014-06-30 | Новозимс А/С | Варианты глюкоамилазы и кодирующие их полинуклеотиды |
| EA201490649A1 (ru) | 2011-10-11 | 2014-07-30 | Новозимс А/С | Варианты глюкоамилазы и кодирующие их полинуклеотиды |
| MX353581B (es) | 2011-10-11 | 2018-01-19 | Novozymes North America Inc | Procesos para producir productos de fermentacion. |
| RU2014121491A (ru) | 2011-10-28 | 2015-12-10 | ДАНИСКО ЮЭс ИНК. | Варианты вариантной альфа-амилазы, образующей мальтогексаозу |
| IN2014CN04905A (https=) | 2011-12-02 | 2015-09-18 | Novozymes As | |
| WO2013083801A2 (en) | 2011-12-09 | 2013-06-13 | Novozymes A/S | Biogas from substrates comprising animal manure and enzymes |
| EP2794899A1 (en) | 2011-12-21 | 2014-10-29 | Novozymes, Inc. | Methods for determining the degradation of a biomass material |
| CN104334738B (zh) | 2012-03-30 | 2019-01-29 | 诺维信北美公司 | 生产发酵产物的方法 |
| US9828595B2 (en) | 2012-08-17 | 2017-11-28 | Novozymes A/S | Thermostable asparaginase variants and polynucleotides encoding same |
| EP2925849A2 (en) * | 2012-11-30 | 2015-10-07 | Novozymes A/S | Polypeptides for cleaning or detergent compositions |
| DK2970931T3 (en) | 2013-03-11 | 2018-01-22 | Danisco Us Inc | COMBINATORY ALPHA AMYLASE VARIATIONS |
| CA2904022C (en) | 2013-04-30 | 2022-04-19 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
| WO2014177541A2 (en) | 2013-04-30 | 2014-11-06 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
| DK3882346T3 (da) | 2013-05-29 | 2025-12-22 | Danisco Us Inc | Hidtil ukendte metalloproteaser |
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- 2000-03-28 DK DK07106717.7T patent/DK1818396T3/da active
- 2000-03-28 AU AU34193/00A patent/AU3419300A/en not_active Abandoned
- 2000-03-28 ES ES00912415T patent/ES2286012T3/es not_active Expired - Lifetime
- 2000-03-28 BR BRPI0009362A patent/BRPI0009362B8/pt active IP Right Grant
- 2000-03-28 RU RU2001129163/13A patent/RU2231547C2/ru active
- 2000-03-28 ES ES07106717.7T patent/ES2496568T3/es not_active Expired - Lifetime
- 2000-03-28 EP EP10181113.1A patent/EP2290060B1/en not_active Expired - Lifetime
- 2000-03-28 KR KR1020017012076A patent/KR100808517B1/ko not_active Expired - Lifetime
- 2000-03-28 AT AT00912415T patent/ATE360686T1/de active
- 2000-03-28 DE DE60034558T patent/DE60034558T2/de not_active Expired - Lifetime
- 2000-03-28 EP EP00912415A patent/EP1165762B1/en not_active Expired - Lifetime
- 2000-03-28 ES ES10181113.1T patent/ES2618608T3/es not_active Expired - Lifetime
- 2000-03-28 EP EP07106717.7A patent/EP1818396B1/en not_active Expired - Lifetime
- 2000-03-30 AR ARP000101431A patent/AR023214A1/es not_active Application Discontinuation
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996023874A1 (en) * | 1995-02-03 | 1996-08-08 | Novo Nordisk A/S | A method of designing alpha-amylase mutants with predetermined properties |
| WO1997041213A1 (en) * | 1996-04-30 | 1997-11-06 | Novo Nordisk A/S | α-AMYLASE MUTANTS |
Also Published As
| Publication number | Publication date |
|---|---|
| DK1818396T3 (da) | 2014-08-11 |
| EP2290060A1 (en) | 2011-03-02 |
| EP1165762B1 (en) | 2007-04-25 |
| ES2286012T3 (es) | 2007-12-01 |
| JP4668426B2 (ja) | 2011-04-13 |
| MXPA01009812A (es) | 2002-06-21 |
| AR023214A1 (es) | 2002-09-04 |
| BR0009362A (pt) | 2001-12-26 |
| WO2000060059A2 (en) | 2000-10-12 |
| KR20010108369A (ko) | 2001-12-07 |
| DK2290060T3 (en) | 2017-03-06 |
| DE60034558D1 (de) | 2007-06-06 |
| BRPI0009362B8 (pt) | 2019-08-20 |
| ES2618608T3 (es) | 2017-06-21 |
| DK1165762T3 (da) | 2007-09-03 |
| BRPI0009362B1 (pt) | 2018-11-21 |
| EP1818396A3 (en) | 2007-12-19 |
| CN1252256C (zh) | 2006-04-19 |
| ATE360686T1 (de) | 2007-05-15 |
| EP1165762A2 (en) | 2002-01-02 |
| CA2365438A1 (en) | 2000-10-12 |
| CA2365438C (en) | 2015-07-14 |
| EP2290060B1 (en) | 2016-12-07 |
| WO2000060059A3 (en) | 2001-05-10 |
| EP1818396B1 (en) | 2014-06-18 |
| CN1346404A (zh) | 2002-04-24 |
| DE60034558T2 (de) | 2007-12-27 |
| RU2231547C2 (ru) | 2004-06-27 |
| ES2496568T3 (es) | 2014-09-19 |
| JP2002540785A (ja) | 2002-12-03 |
| EP1818396A2 (en) | 2007-08-15 |
| AU3419300A (en) | 2000-10-23 |
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| US10113157B2 (en) | Alpha-amylase variants | |
| EP1131418B1 (en) | Alpha-amylase variants | |
| US20150132823A1 (en) | Alpha-Amylase Variants | |
| AU2005202164B2 (en) | Alpha-amylase variants |
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