JPH09511653A - 増幅及び連結反応の共役法 - Google Patents
増幅及び連結反応の共役法Info
- Publication number
- JPH09511653A JPH09511653A JP8508244A JP50824496A JPH09511653A JP H09511653 A JPH09511653 A JP H09511653A JP 8508244 A JP8508244 A JP 8508244A JP 50824496 A JP50824496 A JP 50824496A JP H09511653 A JPH09511653 A JP H09511653A
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- JP
- Japan
- Prior art keywords
- amplification
- annealing temperature
- ligation
- temperature
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Steroid Compounds (AREA)
- Compounds Of Unknown Constitution (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Seal Device For Vehicle (AREA)
- Surgical Instruments (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.試料中の1種以上のポリヌクレオチドを検出する方法であって、下記の工程 を含む方法。 (a)各々が第1アニーリング温度において1種以上の標的ポリヌクレオチ ドに対してアニールすることができる複数の増幅プライマーを供給する工程; (b)各々が第2アニーリング温度において該標的ポリヌクレオチドに対し てアニールすることができ、実質的にいずれも該第1アニーリング温度において 該標的ポリヌクレオチドに対してアニールしない複数のオリゴヌクレオチドプロ ーブを供給する工程; (c)該第1アニーリング温度以上の温度において該複数の増幅プライマー を用いて該標的ポリヌクレオチドを増幅する工程; (d)該第2アニーリング温度以下の温度において該1種以上の標的ポリヌ クレオチドに対して特異的にハイブリッド形成する該複数のオリゴヌクレオチド プローブを結合して1種以上の連結反応産物を形成する工程;及び (e)該1種以上の連結反応産物を検出する工程。 2.前記増幅工程が、ポリメラーゼ連鎖反応又はリガーゼ連鎖反応により増幅す る段階を含む請求項1記載の方法。 3.前記第1アニーリング温度が約72〜約84℃であり、前記第2アニーリン グ温度が約30〜約55℃である請求項2記載の方法。 4.前記第1アニーリング温度が約72〜約75℃であり、前記第2アニーリン グ温度が約40〜約55℃である請求項3記載の方法。 5.前記連結反応産物が異なる電気泳動移動度を有し、前記検出工程が前記連結 反応産物を電気泳動で分離する段階を含む請求項1記載の方法。 6.前記連結反応産物が異なる蛍光標識を有し、前記検出工程が該異なる蛍光標 識で生じた蛍光シグナルを測定する段階を含む請求項1記載の方法。 7.前記連結反応産物が異なる電気泳動移動度を有し、前記検出工程が前記連結 反応産物を電気泳動で分離する段階を含む請求項6記載の方法。 8.前記蛍光標識が、5−カルボキシフルオレセイン、6−カルボキシフルオレ セイン、2′,7′−ジメトキシ−4′,5′−ジクロロ−6−カルボキシフルオ レセイン、N,N,N′,N′−テトラメチル−6−カルボキシローダミン、6 −カルボキシローダミンX、4,7,2′,4′,5′,7′−ヘキサクロロ−6−カルボ キシフルオレセイン、4,7,2′,4′,5′,7′−ヘキサクロロ−5−カルボキシフ ルオレセイン、2′,4′,5′,7′−テトラクロロ−5−カルボキシフルオレセイ ン、4,7,2′,7′−テトラクロロ−6−カルボキシフルオレセイン、1′,2′,7′ ,8′−ジベンゾ-4,7−ジクロロ−5−カルボキシフルオレセイン及び1′,2′,7 ′,8′−ジベンゾ-4,7−ジクロロ−6−カルボキシフルオレセインからなる群よ り選ばれる請求項7記載の方法。 9.前記1種以上の標的ポリヌクレオチドが嚢胞性線維症貫膜電気伝導度調節因 子(CFTR)をコードする遺伝子の対立遺伝子又は突然変異である請求項8記 載の方法。 10.前記1種以上の標的ポリヌクレオチドが、△F508、G542X、G55 1D、W1282X、N1303K、3905insT、3849+10kbC T、3849+4AG、3659de1C、R117H、R1162X、171 7−1GT、621+1GT、R553X、2789+5GA、R347P、2 184de1A、1078de1T、R334W、711+1GT、G85E、 1898+1GA、A455E、S549R、S549N、R560T、△15 07、Q493X、V520F及びY122Xからなる群より選ばれた嚢胞性線 維症貫膜電気伝導度調節因子(CFTR)をコードする遺伝子の対立遺伝子又は 突然変異である請求項9記載の方法。 11.試料中の1種以上のポリヌクレオチドを検出するためのキットであって、下 記の成分を含むキット。 (a)各々が第1アニーリング温度において1種以上の標的ポリヌクレオチ ドに対してアニールすることができる複数の増幅プライマー; (b)各々が第2アニーリング温度において該標的ポリヌクレオチドに対し てアニールすることができ、実質的にいずれも該第1アニーリング温度において 該標的ポリヌクレオチドに対してアニールしない複数のオリゴヌクレオチドプロ ーブ; (c)該第1アニーリング温度以上の温度において該複数の増幅プライマー を用いて該標的ポリヌクレオチドを増幅する手段;及び (d)該第2アニーリング温度以下の温度においてオリゴヌクレオチドプロ ーブを結合して1種以上の連結反応産物を形成する手段。 12.下記の成分を更に含む請求項11記載のキット。 (a)請求項1記載の方法を実施するための説明書; (b)DNAポリメラーゼ; (c)ヌクレオシド三リン酸; (d)DNAリガーゼ:及び (e)増幅及び連結反応の共役用反応バッファー。 13. 前記オリゴヌクレオチドプローブ及び前記増幅プライマーが、△F508 、G542X、G551D、W1282X、N1303K、3905insT、 3849+10kbCT、3849+4AG、3659de1C、R117H、 R1162X、1717−1GT、621+1GT、R553X、2789+5 GA、R347P、2184de1A、1078de1T、R334W、711 +1GT、G85E、1898+1GA、A455E、S549R、S549N 、R560T、△1507、Q493X、V520F及びY122Xからなる群 より選ばれた嚢胞性線維症貫膜電気伝導度調節因子(CFTR)をコードする遺 伝子の対立遺伝子又は突然変異を検出することができる請求項12記載のキット。
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US29268694A | 1994-08-19 | 1994-08-19 | |
US292,686 | 1994-08-19 | ||
US08/292,686 | 1994-08-19 | ||
PCT/US1995/010603 WO1996006190A2 (en) | 1994-08-19 | 1995-08-17 | Coupled amplification and ligation method |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH09511653A true JPH09511653A (ja) | 1997-11-25 |
JP3102800B2 JP3102800B2 (ja) | 2000-10-23 |
Family
ID=23125755
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP08508244A Expired - Lifetime JP3102800B2 (ja) | 1994-08-19 | 1995-08-17 | 増幅及び連結反応の共役法 |
Country Status (7)
Country | Link |
---|---|
US (2) | US5912148A (ja) |
EP (1) | EP0777749B1 (ja) |
JP (1) | JP3102800B2 (ja) |
AT (1) | ATE226983T1 (ja) |
CA (1) | CA2195562A1 (ja) |
DE (1) | DE69528706T2 (ja) |
WO (1) | WO1996006190A2 (ja) |
Cited By (2)
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JP2013527769A (ja) * | 2010-05-06 | 2013-07-04 | アイビス バイオサイエンシズ インコーポレイティッド | 統合された試料調製システムおよび安定化酵素混合物 |
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US4883750A (en) * | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4988617A (en) * | 1988-03-25 | 1991-01-29 | California Institute Of Technology | Method of detecting a nucleotide change in nucleic acids |
US5407769A (en) * | 1989-07-28 | 1995-04-18 | Canon Kabushiki Kaisha | Magnetic toner having triaryl methyl organic resin |
US5407796A (en) * | 1991-01-04 | 1995-04-18 | The Johns Hopkins University | Cystic fibrosis mutation cluster |
US5427932A (en) * | 1991-04-09 | 1995-06-27 | Reagents Of The University Of California | Repeat sequence chromosome specific nucleic acid probes and methods of preparing and using |
ES2091976T3 (es) * | 1991-06-20 | 1996-11-16 | Hoffmann La Roche | Metodos perfeccionados para la amplificacion del acido nucleico. |
WO1993010267A1 (en) * | 1991-11-15 | 1993-05-27 | Igen, Inc. | Rapid assays for amplification products |
DE69322266T2 (de) * | 1992-04-03 | 1999-06-02 | Perkin Elmer Corp | Proben zusammensetzung und verfahren |
AU4788493A (en) * | 1992-07-21 | 1994-02-14 | Imclone Systems Incorporated | Gap-filling nucleic acid amplification and detection |
US5508179A (en) * | 1994-03-18 | 1996-04-16 | Bio-Rad Laboratories, Inc. | Use of deoxyribose nicotinamide adenine dinucleotide to enhance the specificity of NAD+ -dependent ligation reactions |
-
1995
- 1995-08-17 EP EP95929628A patent/EP0777749B1/en not_active Expired - Lifetime
- 1995-08-17 DE DE69528706T patent/DE69528706T2/de not_active Expired - Lifetime
- 1995-08-17 CA CA002195562A patent/CA2195562A1/en not_active Abandoned
- 1995-08-17 WO PCT/US1995/010603 patent/WO1996006190A2/en active IP Right Grant
- 1995-08-17 AT AT95929628T patent/ATE226983T1/de not_active IP Right Cessation
- 1995-08-17 JP JP08508244A patent/JP3102800B2/ja not_active Expired - Lifetime
-
1996
- 1996-09-19 US US08/975,902 patent/US5912148A/en not_active Expired - Lifetime
-
1999
- 1999-02-17 US US09/251,565 patent/US6130073A/en not_active Expired - Lifetime
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2008504028A (ja) * | 2004-06-23 | 2008-02-14 | セクエノム,インコーポレイティド | 標的特異的コンポマー及び使用法 |
JP2013527769A (ja) * | 2010-05-06 | 2013-07-04 | アイビス バイオサイエンシズ インコーポレイティッド | 統合された試料調製システムおよび安定化酵素混合物 |
JP2016182127A (ja) * | 2010-05-06 | 2016-10-20 | アイビス バイオサイエンシズ インコーポレイティッド | 統合された試料調製システムおよび安定化酵素混合物 |
US9737887B2 (en) | 2010-05-06 | 2017-08-22 | Ibis Biosciences, Inc. | Integrated sample preparation systems and stabilized enzyme mixtures |
Also Published As
Publication number | Publication date |
---|---|
ATE226983T1 (de) | 2002-11-15 |
CA2195562A1 (en) | 1996-02-29 |
AU3332295A (en) | 1996-03-14 |
EP0777749A2 (en) | 1997-06-11 |
WO1996006190A2 (en) | 1996-02-29 |
AU699676B2 (en) | 1998-12-10 |
US5912148A (en) | 1999-06-15 |
DE69528706T2 (de) | 2003-06-12 |
US6130073A (en) | 2000-10-10 |
DE69528706D1 (de) | 2002-12-05 |
WO1996006190A3 (en) | 1996-05-09 |
JP3102800B2 (ja) | 2000-10-23 |
EP0777749B1 (en) | 2002-10-30 |
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