JPH0561920B2 - - Google Patents
Info
- Publication number
- JPH0561920B2 JPH0561920B2 JP62042442A JP4244287A JPH0561920B2 JP H0561920 B2 JPH0561920 B2 JP H0561920B2 JP 62042442 A JP62042442 A JP 62042442A JP 4244287 A JP4244287 A JP 4244287A JP H0561920 B2 JPH0561920 B2 JP H0561920B2
- Authority
- JP
- Japan
- Prior art keywords
- fragment
- hind
- nucleic acid
- restriction enzyme
- probe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000523 sample Substances 0.000 claims description 145
- 150000007523 nucleic acids Chemical class 0.000 claims description 91
- 108020004707 nucleic acids Proteins 0.000 claims description 90
- 102000039446 nucleic acids Human genes 0.000 claims description 90
- 238000012360 testing method Methods 0.000 claims description 81
- 239000012634 fragment Substances 0.000 claims description 61
- 108091008146 restriction endonucleases Proteins 0.000 claims description 57
- 108090000623 proteins and genes Proteins 0.000 claims description 45
- 238000009396 hybridization Methods 0.000 claims description 40
- 239000013612 plasmid Substances 0.000 claims description 39
- 108020004414 DNA Proteins 0.000 claims description 38
- 238000000034 method Methods 0.000 claims description 33
- 239000013598 vector Substances 0.000 claims description 21
- 239000003153 chemical reaction reagent Substances 0.000 claims description 12
- 108700024542 myc Genes Proteins 0.000 claims description 11
- 230000003321 amplification Effects 0.000 claims description 10
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 10
- 101150074155 DHFR gene Proteins 0.000 claims description 9
- 108010022394 Threonine synthase Proteins 0.000 claims description 9
- 102000004419 dihydrofolate reductase Human genes 0.000 claims description 9
- 108020004999 messenger RNA Proteins 0.000 claims description 9
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 108700020796 Oncogene Proteins 0.000 claims description 7
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims description 6
- 238000003556 assay Methods 0.000 claims description 6
- 238000011144 upstream manufacturing Methods 0.000 claims description 6
- 102000053602 DNA Human genes 0.000 claims description 5
- 241000713333 Mouse mammary tumor virus Species 0.000 claims description 5
- 101710135898 Myc proto-oncogene protein Proteins 0.000 claims description 5
- 102100038895 Myc proto-oncogene protein Human genes 0.000 claims description 5
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 claims description 5
- 101710150448 Transcriptional regulator Myc Proteins 0.000 claims description 5
- 238000004445 quantitative analysis Methods 0.000 claims description 4
- 108700042226 ras Genes Proteins 0.000 claims description 4
- 229920002477 rna polymer Polymers 0.000 claims description 4
- 239000013600 plasmid vector Substances 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 2
- 101000584612 Homo sapiens GTPase KRas Proteins 0.000 claims 3
- 108700026220 vif Genes Proteins 0.000 claims 2
- 230000004544 DNA amplification Effects 0.000 claims 1
- 102100024746 Dihydrofolate reductase Human genes 0.000 claims 1
- 108020001096 dihydrofolate reductase Proteins 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 39
- 238000005259 measurement Methods 0.000 description 24
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 19
- 239000000243 solution Substances 0.000 description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 239000002299 complementary DNA Substances 0.000 description 7
- 239000000020 Nitrocellulose Substances 0.000 description 6
- 229920001220 nitrocellulos Polymers 0.000 description 6
- 238000011002 quantification Methods 0.000 description 6
- 206010028980 Neoplasm Diseases 0.000 description 5
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 5
- 239000012445 acidic reagent Substances 0.000 description 5
- 201000011510 cancer Diseases 0.000 description 5
- 101150026150 mt gene Proteins 0.000 description 5
- 239000002773 nucleotide Substances 0.000 description 5
- 125000003729 nucleotide group Chemical group 0.000 description 5
- 238000013519 translation Methods 0.000 description 5
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 238000013518 transcription Methods 0.000 description 4
- 230000035897 transcription Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical group N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- CKTSBUTUHBMZGZ-SHYZEUOFSA-N 2'‐deoxycytidine Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-SHYZEUOFSA-N 0.000 description 2
- CKTSBUTUHBMZGZ-UHFFFAOYSA-N Deoxycytidine Natural products O=C1N=C(N)C=CN1C1OC(CO)C(O)C1 CKTSBUTUHBMZGZ-UHFFFAOYSA-N 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 208000026350 Inborn Genetic disease Diseases 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 102000052708 Recessive Genes Human genes 0.000 description 2
- 108700005079 Recessive Genes Proteins 0.000 description 2
- 108020004511 Recombinant DNA Proteins 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 208000016361 genetic disease Diseases 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000002777 nucleoside Substances 0.000 description 2
- -1 nucleoside triphosphate Chemical class 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 239000001226 triphosphate Substances 0.000 description 2
- 235000011178 triphosphate Nutrition 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 108091093088 Amplicon Proteins 0.000 description 1
- 206010008805 Chromosomal abnormalities Diseases 0.000 description 1
- 208000031404 Chromosome Aberrations Diseases 0.000 description 1
- 241000252203 Clupea harengus Species 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- 201000010374 Down Syndrome Diseases 0.000 description 1
- 102100030708 GTPase KRas Human genes 0.000 description 1
- 101710113436 GTPase KRas Proteins 0.000 description 1
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 108700019961 Neoplasm Genes Proteins 0.000 description 1
- 102000048850 Neoplasm Genes Human genes 0.000 description 1
- 206010029260 Neuroblastoma Diseases 0.000 description 1
- 102000043276 Oncogene Human genes 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000011712 cell development Effects 0.000 description 1
- 108091092356 cellular DNA Proteins 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000008175 fetal development Effects 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 235000019514 herring Nutrition 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 229960000485 methotrexate Drugs 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 238000003793 prenatal diagnosis Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 102000016914 ras Proteins Human genes 0.000 description 1
- 108010014186 ras Proteins Proteins 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 208000000587 small cell lung carcinoma Diseases 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 108010051423 streptavidin-agarose Proteins 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI860836A FI76119C (fi) | 1986-02-27 | 1986-02-27 | Kvantitativ bestaemning av nukleinsyramolekyler och reagensfoerpackning som anvaends vid foerfarandet. |
| FI860836 | 1986-02-27 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62205800A JPS62205800A (ja) | 1987-09-10 |
| JPH0561920B2 true JPH0561920B2 (hu) | 1993-09-07 |
Family
ID=8522222
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62042442A Granted JPS62205800A (ja) | 1986-02-27 | 1987-02-25 | 核酸分子の定量法およびそれに用いる試薬 |
Country Status (26)
| Country | Link |
|---|---|
| JP (1) | JPS62205800A (hu) |
| KR (1) | KR870008033A (hu) |
| AT (1) | AT393511B (hu) |
| AU (1) | AU603562B2 (hu) |
| BE (1) | BE1001168A4 (hu) |
| CA (1) | CA1287558C (hu) |
| CH (1) | CH675593A5 (hu) |
| DD (1) | DD270383A5 (hu) |
| DE (1) | DE3706285A1 (hu) |
| DK (1) | DK174784B1 (hu) |
| ES (1) | ES2061411A6 (hu) |
| FI (1) | FI76119C (hu) |
| FR (1) | FR2594849B1 (hu) |
| GB (1) | GB2187283B (hu) |
| HU (1) | HU201808B (hu) |
| IE (1) | IE66904B1 (hu) |
| IL (1) | IL81695A (hu) |
| IS (1) | IS3197A7 (hu) |
| IT (1) | IT1202581B (hu) |
| LU (1) | LU86792A1 (hu) |
| NL (1) | NL195097C (hu) |
| NO (1) | NO175380C (hu) |
| NZ (1) | NZ219421A (hu) |
| PT (1) | PT84368B (hu) |
| SE (1) | SE468816B (hu) |
| ZA (1) | ZA871401B (hu) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5714380A (en) | 1986-10-23 | 1998-02-03 | Amoco Corporation | Closed vessel for isolating target molecules and for performing amplification |
| IL86724A (en) | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
| EP0304845A3 (en) * | 1987-08-28 | 1991-03-06 | Profile Diagnostic Sciences Inc. | Method and kit for assaying gene expressions |
| DE68921918T2 (de) * | 1988-05-09 | 1995-09-07 | Univ Temple | Verfahren zur Voraussage der Wirksamkeit einer antineoplastichen Behandlung bei einzelnen Patienten. |
| AU629845B2 (en) * | 1988-08-30 | 1992-10-15 | Abbott Laboratories | Detection and amplification of target nucleic acid sequences |
| WO1990014440A1 (en) * | 1989-05-18 | 1990-11-29 | The United States Of America, Represented By The Secretary, United States Department Of Commerce | RNA PROBE FOR DETECTING c-fes mRNA |
| US5232829A (en) * | 1989-09-29 | 1993-08-03 | Hoffmann-La Roche Inc. | Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes |
| DK138090D0 (da) * | 1990-06-06 | 1990-06-06 | Novo Nordisk As | Diagnostisk analysemetode |
| ATE143700T1 (de) * | 1991-09-23 | 1996-10-15 | Pfizer | Verfahren für die detektion von spezifische mrns und dns in zellen |
| US6300058B1 (en) | 1992-01-29 | 2001-10-09 | Hitachi Chemical Research Center, Inc. | Method for measuring messenger RNA |
| GB9210916D0 (en) * | 1992-05-21 | 1992-07-08 | Isis Innovation | Nucleic acid quantification |
| MX9300494A (es) * | 1992-07-28 | 1994-07-29 | Hitachi Chemical Co Ltd | Metodo para la deteccion genetica de organismos, agentes infecciosos o componentes de una celula u organismo en una muestra biologica. |
| US5580971A (en) * | 1992-07-28 | 1996-12-03 | Hitachi Chemical Company, Ltd. | Fungal detection system based on rRNA probes |
| ES2055661B1 (es) * | 1993-01-20 | 1995-03-01 | Univ Malaga | Determinacion de la expresion genica por captura especifica de arn y su cuantificacion directa por electroforesis capilar en zona libre. |
| GB9309966D0 (en) * | 1993-05-14 | 1993-06-30 | Nordion Int Inc | Detection of prostrate-specific antigen in breast tumors |
| GB9415489D0 (en) * | 1994-08-01 | 1994-09-21 | Nordion Int Inc | Detection of prostate-specific antigen in amniotic fluid |
| AT401062B (de) * | 1994-09-26 | 1996-06-25 | Immuno Ag | Verfahren zur quantifizierung von nukleinsäuren |
| WO2011122034A1 (ja) * | 2010-03-31 | 2011-10-06 | 有限会社山口ティー・エル・オー | 核酸クロマトグラフ法を利用した肺炎原因菌の検出方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4442203A (en) * | 1981-06-30 | 1984-04-10 | Massachusetts Institute Of Technology | Gene amplification assay for detecting tumor promoters |
| FI63596C (fi) * | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
| AU555146B2 (en) * | 1982-03-15 | 1986-09-11 | Trustees Of Columbia University In The City Of New York, The | Method for intorducing cloned, amplifiable genes into eucaryotic cells and for producing proteinaceous products materials |
| IE56509B1 (en) * | 1982-11-04 | 1991-08-28 | Univ California | Methods for oncogenic detection |
| FI71768C (fi) * | 1984-02-17 | 1987-02-09 | Orion Yhtymae Oy | Foerbaettrade nykleinsyrareagenser och foerfarande foer deras framstaellning. |
| US4675283A (en) * | 1984-07-19 | 1987-06-23 | Massachusetts Institute Of Technology | Detection and isolation of homologous, repeated and amplified nucleic acid sequences |
| FI72146C (fi) * | 1985-01-02 | 1987-04-13 | Orion Yhtymae Oy | Foerfarande foer identifiering av nukleinsyror. |
| FR2583771B1 (fr) * | 1985-06-21 | 1988-12-09 | Centre Nat Rech Scient | Sequences d'adn d'archaebacteries homologues a l'oncogene v-myc, proteines codees par ces sequences, leurs procedes d'obtention et leurs applications immunologiques |
-
1986
- 1986-02-27 FI FI860836A patent/FI76119C/fi not_active IP Right Cessation
-
1987
- 1987-02-25 JP JP62042442A patent/JPS62205800A/ja active Granted
- 1987-02-26 CH CH756/87A patent/CH675593A5/de not_active IP Right Cessation
- 1987-02-26 NL NL8700483A patent/NL195097C/nl not_active IP Right Cessation
- 1987-02-26 LU LU86792A patent/LU86792A1/fr unknown
- 1987-02-26 BE BE8700177A patent/BE1001168A4/fr not_active IP Right Cessation
- 1987-02-26 PT PT84368A patent/PT84368B/pt unknown
- 1987-02-26 IS IS3197A patent/IS3197A7/is unknown
- 1987-02-26 HU HU87750A patent/HU201808B/hu unknown
- 1987-02-26 DE DE19873706285 patent/DE3706285A1/de active Granted
- 1987-02-26 GB GB8704517A patent/GB2187283B/en not_active Expired - Lifetime
- 1987-02-26 IT IT19501/87A patent/IT1202581B/it active
- 1987-02-26 FR FR878702541A patent/FR2594849B1/fr not_active Expired - Lifetime
- 1987-02-26 ZA ZA871401A patent/ZA871401B/xx unknown
- 1987-02-26 DK DK198700993A patent/DK174784B1/da not_active IP Right Cessation
- 1987-02-26 AT AT431/87A patent/AT393511B/de not_active IP Right Cessation
- 1987-02-26 AU AU69275/87A patent/AU603562B2/en not_active Expired
- 1987-02-26 SE SE8700821A patent/SE468816B/sv not_active IP Right Cessation
- 1987-02-26 KR KR870001680A patent/KR870008033A/ko not_active Application Discontinuation
- 1987-02-26 NO NO870794A patent/NO175380C/no unknown
- 1987-02-26 NZ NZ219421A patent/NZ219421A/xx unknown
- 1987-02-26 CA CA000530691A patent/CA1287558C/en not_active Expired - Lifetime
- 1987-02-26 ES ES08700776A patent/ES2061411A6/es not_active Expired - Fee Related
- 1987-02-26 IE IE49687A patent/IE66904B1/en not_active IP Right Cessation
- 1987-02-27 IL IL81695A patent/IL81695A/xx not_active IP Right Cessation
- 1987-02-27 DD DD87300286A patent/DD270383A5/de unknown
Also Published As
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