JP6998319B2 - 対流流体装置における核酸の表面ベースの検出方法 - Google Patents

対流流体装置における核酸の表面ベースの検出方法 Download PDF

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JP6998319B2
JP6998319B2 JP2018551295A JP2018551295A JP6998319B2 JP 6998319 B2 JP6998319 B2 JP 6998319B2 JP 2018551295 A JP2018551295 A JP 2018551295A JP 2018551295 A JP2018551295 A JP 2018551295A JP 6998319 B2 JP6998319 B2 JP 6998319B2
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oligonucleotide
dna sequence
reaction chamber
dna
surface region
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JP2019509751A5 (enExample
JP2019509751A (ja
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ドミトリー エー ホダコフ
デビッド チャン
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Rice University
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2018551295A 2016-03-29 2017-03-28 対流流体装置における核酸の表面ベースの検出方法 Active JP6998319B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2021205691A JP2022037151A (ja) 2016-03-29 2021-12-20 対流流体装置における核酸の表面ベースの検出方法
JP2023192003A JP2023184748A (ja) 2016-03-29 2023-11-10 対流流体装置における核酸の表面ベースの検出方法

Applications Claiming Priority (3)

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US201662314909P 2016-03-29 2016-03-29
US62/314,909 2016-03-29
PCT/US2017/024530 WO2017172760A1 (en) 2016-03-29 2017-03-28 Surface-based detection of nucleic acid in a convection flow fluidic device

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JP2019509751A JP2019509751A (ja) 2019-04-11
JP2019509751A5 JP2019509751A5 (enExample) 2020-04-23
JP6998319B2 true JP6998319B2 (ja) 2022-01-18

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JP2021205691A Withdrawn JP2022037151A (ja) 2016-03-29 2021-12-20 対流流体装置における核酸の表面ベースの検出方法
JP2023192003A Pending JP2023184748A (ja) 2016-03-29 2023-11-10 対流流体装置における核酸の表面ベースの検出方法

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JP2023192003A Pending JP2023184748A (ja) 2016-03-29 2023-11-10 対流流体装置における核酸の表面ベースの検出方法

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US (2) US20200324292A1 (enExample)
EP (2) EP4063014A1 (enExample)
JP (3) JP6998319B2 (enExample)
KR (2) KR102472071B1 (enExample)
CN (1) CN109477136A (enExample)
AU (2) AU2017241766A1 (enExample)
BR (1) BR112018070197A2 (enExample)
DK (1) DK3436198T3 (enExample)
ES (1) ES2920954T3 (enExample)
IL (1) IL261980B2 (enExample)
PH (1) PH12018502100A1 (enExample)
PT (1) PT3436198T (enExample)
RU (1) RU2018137800A (enExample)
SG (1) SG11201808493QA (enExample)
WO (1) WO2017172760A1 (enExample)

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US12599908B2 (en) * 2018-09-14 2026-04-14 William Marsh Rice University Apparatus and methods for multiplexed amplification and detection of DNA using convectional heating and label-free microarray
CN113257351A (zh) * 2020-02-12 2021-08-13 赛纳生物科技(北京)有限公司 一种用于多碱基基因测序的基因文库及其构建方法
CN111879922A (zh) * 2020-07-13 2020-11-03 东南大学 适用于核酸及免疫同步检测集成纸基芯片结构及制造方法
DE102020212253A1 (de) * 2020-09-29 2022-03-31 SpinDiag GmbH Probenträger und Rotationsvorrichtung
EP4640315A1 (en) * 2024-04-26 2025-10-29 Appolon Bioteck Devices and methods for rapid nucleic acid amplification

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JP2010534328A (ja) 2007-07-23 2010-11-04 クロンデイアグ・ゲーエムベーハー アッセイ
JP2013540451A (ja) 2010-10-27 2013-11-07 プレジデント アンド フェロウズ オブ ハーバード カレッジ トーホールドプライマーデュプレックスの組成物およびその使用方法
US20140302486A1 (en) 2011-09-02 2014-10-09 President And Fellows Of Harvard College Systems and methods for detecting biomarkers of interest
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WO2015094429A1 (en) 2013-12-16 2015-06-25 William Marsh Rice University Fine-tuned ultraspecific nucleic acid hybridization probes

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JP2003522963A (ja) * 2000-02-18 2003-07-29 アクララ バイオサイエンシーズ， インコーポレイテッド 複数部位反応デバイスおよび方法
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JP2010534328A (ja) 2007-07-23 2010-11-04 クロンデイアグ・ゲーエムベーハー アッセイ
US20100056397A1 (en) 2008-08-27 2010-03-04 Joo-Won Rhee Method of Producing Microarray Having Immobilized Double-Stranded Nucleic Acid Probe Including Double-Stranded Region and Single-Stranded Region
JP2013540451A (ja) 2010-10-27 2013-11-07 プレジデント アンド フェロウズ オブ ハーバード カレッジ トーホールドプライマーデュプレックスの組成物およびその使用方法
US20140302486A1 (en) 2011-09-02 2014-10-09 President And Fellows Of Harvard College Systems and methods for detecting biomarkers of interest
WO2015037255A1 (ja) 2013-09-11 2015-03-19 国立大学法人大阪大学 熱対流生成用チップ、熱対流生成装置、及び熱対流生成方法
WO2015094429A1 (en) 2013-12-16 2015-06-25 William Marsh Rice University Fine-tuned ultraspecific nucleic acid hybridization probes

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US20200324292A1 (en) 2020-10-15
US20240278249A1 (en) 2024-08-22
KR20190021198A (ko) 2019-03-05
JP2022037151A (ja) 2022-03-08
RU2018137800A3 (enExample) 2020-06-18
EP3436198A4 (en) 2019-10-30
PT3436198T (pt) 2022-07-13
PH12018502100A1 (en) 2019-07-29
IL261980A (en) 2018-10-31
CA3019422A1 (en) 2017-10-05
RU2018137800A (ru) 2020-04-29
BR112018070197A2 (pt) 2019-01-29
EP3436198B1 (en) 2022-05-04
JP2019509751A (ja) 2019-04-11
EP4063014A1 (en) 2022-09-28
IL261980B1 (en) 2023-04-01
JP2023184748A (ja) 2023-12-28
AU2023203972A1 (en) 2023-07-13
ES2920954T3 (es) 2022-08-12
SG11201808493QA (en) 2018-10-30
KR102472071B1 (ko) 2022-11-29
AU2017241766A1 (en) 2018-10-25
WO2017172760A1 (en) 2017-10-05
KR20230004792A (ko) 2023-01-06
KR102789837B1 (ko) 2025-04-01
IL261980B2 (en) 2023-08-01
CN109477136A (zh) 2019-03-15
DK3436198T3 (en) 2022-06-27
EP3436198A1 (en) 2019-02-06

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