BR112018070197A2 - detecção baseada na superfície de ácidos nucleicos em dispositivos fluidos de fluxo de convecção - Google Patents

detecção baseada na superfície de ácidos nucleicos em dispositivos fluidos de fluxo de convecção

Info

Publication number
BR112018070197A2
BR112018070197A2 BR112018070197A BR112018070197A BR112018070197A2 BR 112018070197 A2 BR112018070197 A2 BR 112018070197A2 BR 112018070197 A BR112018070197 A BR 112018070197A BR 112018070197 A BR112018070197 A BR 112018070197A BR 112018070197 A2 BR112018070197 A2 BR 112018070197A2
Authority
BR
Brazil
Prior art keywords
nucleic acids
based detection
flow devices
convection flow
fluid convection
Prior art date
Application number
BR112018070197A
Other languages
English (en)
Inventor
Zhang David
A Khodakov Dmitriy
Original Assignee
Univ Rice William M
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rice William M filed Critical Univ Rice William M
Publication of BR112018070197A2 publication Critical patent/BR112018070197A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

a presente revelação apresenta métodos, composições e dispositivos para realizar pcr com base em convecção e amplificação não enzimática de sequências de ácidos nucleicos. as técnicas e reagentes empregados nestes métodos incluem sondas toehold, reações de deslocamento de fitas, convecção de rayleigh benard, gradientes de temperatura, amplificação multiplexada, detecção multiplexada e funcionalização do dna, em sistemas abertos e fechados, para uso em testes e ensaios nucleicos.
BR112018070197A 2016-03-29 2017-03-28 detecção baseada na superfície de ácidos nucleicos em dispositivos fluidos de fluxo de convecção BR112018070197A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662314909P 2016-03-29 2016-03-29
PCT/US2017/024530 WO2017172760A1 (en) 2016-03-29 2017-03-28 Surface-based detection of nucleic acid in a convection flow fluidic device

Publications (1)

Publication Number Publication Date
BR112018070197A2 true BR112018070197A2 (pt) 2019-01-29

Family

ID=59965134

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018070197A BR112018070197A2 (pt) 2016-03-29 2017-03-28 detecção baseada na superfície de ácidos nucleicos em dispositivos fluidos de fluxo de convecção

Country Status (16)

Country Link
US (1) US20200324292A1 (pt)
EP (2) EP4063014A1 (pt)
JP (3) JP6998319B2 (pt)
KR (2) KR20230004792A (pt)
CN (1) CN109477136A (pt)
AU (2) AU2017241766A1 (pt)
BR (1) BR112018070197A2 (pt)
CA (1) CA3019422A1 (pt)
DK (1) DK3436198T3 (pt)
ES (1) ES2920954T3 (pt)
IL (1) IL261980B2 (pt)
PH (1) PH12018502100A1 (pt)
PT (1) PT3436198T (pt)
RU (1) RU2018137800A (pt)
SG (1) SG11201808493QA (pt)
WO (1) WO2017172760A1 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020056292A1 (en) * 2018-09-14 2020-03-19 William Marsh Rice University Apparatus and methods for multiplexed amplification and detection of dna using convectional heating and label-free microarray
CN113257351A (zh) * 2020-02-12 2021-08-13 赛纳生物科技(北京)有限公司 一种用于多碱基基因测序的基因文库及其构建方法
CN111879922A (zh) * 2020-07-13 2020-11-03 东南大学 适用于核酸及免疫同步检测集成纸基芯片结构及制造方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027880A (en) 1995-08-02 2000-02-22 Affymetrix, Inc. Arrays of nucleic acid probes and methods of using the same for detecting cystic fibrosis
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US6642000B1 (en) 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides
WO2001061041A2 (en) * 2000-02-18 2001-08-23 Aclara Biosciences Inc. Multiple-site reaction device and method
US6586233B2 (en) 2001-03-09 2003-07-01 The Regents Of The University Of California Convectively driven PCR thermal-cycling
US7846656B2 (en) 2001-10-16 2010-12-07 Institut Molekulyarnoi Biologii Im.V.A. Engelgardta Rossiiskoi Akademii Nauk Composition for polymerizing immobilization of biological molecules and method for producing said composition
KR100647289B1 (ko) * 2004-09-15 2006-11-23 삼성전자주식회사 마랑고니 대류를 이용한 ｐｃｒ 장치 및 이를 이용한ｐｃｒ 방법
CA2624634A1 (en) * 2005-10-03 2007-04-12 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
US8114636B2 (en) * 2006-02-10 2012-02-14 Life Technologies Corporation Labeling and detection of nucleic acids
US8735103B2 (en) 2006-12-05 2014-05-27 Electronics And Telecommunications Research Institute Natural convection-driven PCR apparatus and method using disposable polymer chip
US8110353B2 (en) 2007-02-05 2012-02-07 California Institute Of Technology Engineered toehold reactions and networks
US8043810B2 (en) 2007-05-02 2011-10-25 Eagle Eye Research, Inc. Analyte detection using autocatalytic chain reactions
EP3527288B1 (en) * 2007-07-23 2021-05-12 Abbott Rapid Diagnostics Jena GmbH Methods for virus detection
US8187813B2 (en) 2008-01-24 2012-05-29 Medigen Biotechnology Corp. Methods and apparatuses for convective polymerase chain reaction (PCR)
KR20100025328A (ko) * 2008-08-27 2010-03-09 삼성전자주식회사 이중가닥 영역과 말단 단일가닥 영역을 포함하는 이중가닥 핵산 프로브가 고정된 마이크로어레이를 제조하는 방법
JP5848337B2 (ja) * 2010-05-27 2016-01-27 エメラルド セラピューティクス，インコーポレーテッド 修飾した核酸を用いる情報伝播のシステムおよび方法
ES2774447T3 (es) * 2010-10-27 2020-07-21 Harvard College Métodos de uso de cebador de horquilla de extremos cohesivos
US20140302486A1 (en) * 2011-09-02 2014-10-09 President And Fellows Of Harvard College Systems and methods for detecting biomarkers of interest
JP6427753B2 (ja) * 2013-09-11 2018-11-28 国立大学法人大阪大学 熱対流生成用チップ、熱対流生成装置、及び熱対流生成方法
KR102242192B1 (ko) * 2013-12-16 2021-04-20 윌리엄 마쉬 라이스 유니버시티 미세 조정된 초특정 핵산 하이브리다이제이션 프로브

Also Published As

Publication number Publication date
SG11201808493QA (en) 2018-10-30
DK3436198T3 (en) 2022-06-27
IL261980B1 (en) 2023-04-01
CN109477136A (zh) 2019-03-15
CA3019422A1 (en) 2017-10-05
RU2018137800A3 (pt) 2020-06-18
RU2018137800A (ru) 2020-04-29
PH12018502100A1 (en) 2019-07-29
IL261980A (en) 2018-10-31
JP2023184748A (ja) 2023-12-28
JP2022037151A (ja) 2022-03-08
JP6998319B2 (ja) 2022-01-18
WO2017172760A1 (en) 2017-10-05
PT3436198T (pt) 2022-07-13
KR102472071B1 (ko) 2022-11-29
IL261980B2 (en) 2023-08-01
US20200324292A1 (en) 2020-10-15
KR20190021198A (ko) 2019-03-05
ES2920954T3 (es) 2022-08-12
EP3436198A4 (en) 2019-10-30
JP2019509751A (ja) 2019-04-11
KR20230004792A (ko) 2023-01-06
EP4063014A1 (en) 2022-09-28
EP3436198A1 (en) 2019-02-06
EP3436198B1 (en) 2022-05-04
AU2023203972A1 (en) 2023-07-13
AU2017241766A1 (en) 2018-10-25

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Legal Events

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B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements