SG11201808493QA - Surface-based detection of nucleic acid in a convection flow fluidic device - Google Patents
Surface-based detection of nucleic acid in a convection flow fluidic deviceInfo
- Publication number
- SG11201808493QA SG11201808493QA SG11201808493QA SG11201808493QA SG11201808493QA SG 11201808493Q A SG11201808493Q A SG 11201808493QA SG 11201808493Q A SG11201808493Q A SG 11201808493QA SG 11201808493Q A SG11201808493Q A SG 11201808493QA SG 11201808493Q A SG11201808493Q A SG 11201808493QA
- Authority
- SG
- Singapore
- Prior art keywords
- international
- nucleic acid
- houston
- main
- convection
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 238000000034 method Methods 0.000 abstract 3
- 230000003321 amplification Effects 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 238000003556 assay Methods 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000006073 displacement reaction Methods 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 238000007306 functionalization reaction Methods 0.000 abstract 1
- 230000008520 organization Effects 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0663—Stretching or orienting elongated molecules or particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Clinical Laboratory Science (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Dispersion Chemistry (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 11M11111011101 11111111110101111101110101110101111001111111111111110111111 International Bureau 0.. .... .. (10) International Publication Number ..... ..or::,,, (43) International Publication Date WO 2017/172760 Al 5 October 2017 (05.10.2017) IN I PO I P CT (51) International Patent Classification: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, BOIL 7/00 (2006.01) C12Q 1/68 (2006.01) DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, (21) International Application Number: KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, PCT/US2017/024530 MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, (22) International Filing Date: NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, 28 March 2017 (28.03.2017) RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, (25) Filing Language: English ZA, ZM, ZW. (26) Publication Language: English (84) Designated States (unless otherwise indicated, for every (30) Priority Data: kind of regional protection available): ARIPO (BW, GH, 62/314,909 29 March 2016 (29.03.2016) US GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (71) Applicant: WILLIAM MARSH RICE UNIVERSITY TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, [US/US]; 6100 Main St., Houston, TX 77005 (US). DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, (72) Inventors: KHODAKOV, Dmitriy, A; c/o William Marsh LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, Rice University, 6100 Main St., MS-142, Houston, TX SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, 77005 (US). ZHANG, David; c/o William Marsh Rice GW, KM, ML, MR, NE, SN, TD, TG). University, 6500 Main St., Suite 425, Houston, TX 77030 Published: (US). — with international search report (Art. 21(3)) (74) Agent: HIGHLANDER, Steven, L.; Parker Highlander before the expiration of the time limit for amending the PLLC, 1120 S. Capital Of Texas Highway, Building One, claims and to be republished in the event of receipt of Suite 200, Austin, TX 78746 (US). amendments (Rule 48.2(h)) Designated States (unless otherwise indicated, for every _ with sequence listing part of description (Rule 5.2(a)) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (81) — Il © IN N IN Il IN 1-1 (54) Title: SURFACE-BASED DETECTION OF NUCLEIC ACID IN A CONVECTION FLOW FLUIDIC DEVICE 0 N (57) : The present disclosure provides methods, composition and devices for performing convection-based PCR and non- ea.. ) enzymatic amplification of nucleic acid sequences. Techniques and reagents employed in these methods include toehold probes, ,7 1 .1 . 9 strand displacement reactions, Rayleigh-Benard convection, temperature gradients, multiplexed amplification, multiplexed detection, and DNA functionalization, in open and closed systems, for use in nucleic tests and assays.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662314909P | 2016-03-29 | 2016-03-29 | |
PCT/US2017/024530 WO2017172760A1 (en) | 2016-03-29 | 2017-03-28 | Surface-based detection of nucleic acid in a convection flow fluidic device |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11201808493QA true SG11201808493QA (en) | 2018-10-30 |
Family
ID=59965134
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201808493QA SG11201808493QA (en) | 2016-03-29 | 2017-03-28 | Surface-based detection of nucleic acid in a convection flow fluidic device |
Country Status (16)
Country | Link |
---|---|
US (1) | US20200324292A1 (en) |
EP (2) | EP4063014A1 (en) |
JP (3) | JP6998319B2 (en) |
KR (2) | KR102472071B1 (en) |
CN (1) | CN109477136A (en) |
AU (2) | AU2017241766A1 (en) |
BR (1) | BR112018070197A2 (en) |
CA (1) | CA3019422A1 (en) |
DK (1) | DK3436198T3 (en) |
ES (1) | ES2920954T3 (en) |
IL (1) | IL261980B2 (en) |
PH (1) | PH12018502100A1 (en) |
PT (1) | PT3436198T (en) |
RU (1) | RU2018137800A (en) |
SG (1) | SG11201808493QA (en) |
WO (1) | WO2017172760A1 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20210060500A (en) * | 2018-09-14 | 2021-05-26 | 윌리엄 마쉬 라이스 유니버시티 | Apparatus and method for multiple amplification and detection of DNA using convective heating and label-free microarray |
CN113257351A (en) * | 2020-02-12 | 2021-08-13 | 赛纳生物科技(北京)有限公司 | Gene library for multi-base gene sequencing and construction method thereof |
CN111879922A (en) * | 2020-07-13 | 2020-11-03 | 东南大学 | Integrated paper-based chip structure suitable for synchronous detection of nucleic acid and immunity and manufacturing method thereof |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6027880A (en) | 1995-08-02 | 2000-02-22 | Affymetrix, Inc. | Arrays of nucleic acid probes and methods of using the same for detecting cystic fibrosis |
US6300070B1 (en) | 1999-06-04 | 2001-10-09 | Mosaic Technologies, Inc. | Solid phase methods for amplifying multiple nucleic acids |
US6642000B1 (en) | 1999-11-12 | 2003-11-04 | University Of Chicago | PCR amplification on microarrays of gel immobilized oligonucleotides |
EP1259324A2 (en) * | 2000-02-18 | 2002-11-27 | Aclara BioSciences, Inc. | Multiple-site reaction device and method |
US6586233B2 (en) | 2001-03-09 | 2003-07-01 | The Regents Of The University Of California | Convectively driven PCR thermal-cycling |
WO2003033539A1 (en) | 2001-10-16 | 2003-04-24 | Institut Molekulyarnoi Biologii Im. V.A. Engelgardta Rossiiskoi Akademii Nauk | Composition for polymerising immobilisation of biological molecules and method for producing said composition |
KR100647289B1 (en) * | 2004-09-15 | 2006-11-23 | 삼성전자주식회사 | PCR device using Marangoni convection and method using the device |
JP5438320B2 (en) * | 2005-10-03 | 2014-03-12 | アプライド バイオシステムズ リミテッド ライアビリティー カンパニー | Compositions, methods and kits for amplifying nucleic acids |
US8114636B2 (en) * | 2006-02-10 | 2012-02-14 | Life Technologies Corporation | Labeling and detection of nucleic acids |
US8735103B2 (en) | 2006-12-05 | 2014-05-27 | Electronics And Telecommunications Research Institute | Natural convection-driven PCR apparatus and method using disposable polymer chip |
US8110353B2 (en) | 2007-02-05 | 2012-02-07 | California Institute Of Technology | Engineered toehold reactions and networks |
US8043810B2 (en) | 2007-05-02 | 2011-10-25 | Eagle Eye Research, Inc. | Analyte detection using autocatalytic chain reactions |
CA3006347A1 (en) | 2007-07-23 | 2009-01-29 | Clondiag Gmbh | Methods and devices for determining values indicative of the presence and/or amount of nucleic acids |
WO2009094638A2 (en) | 2008-01-24 | 2009-07-30 | Medigen Biotechnology Corp. | Methods and apparatuses for convective polymerase chain reaction (pcr) |
KR20100025328A (en) * | 2008-08-27 | 2010-03-09 | 삼성전자주식회사 | Method of producing microarray having immoblized double strand nucleic acids comprising double strand region and terminal single strand region |
JP5848337B2 (en) | 2010-05-27 | 2016-01-27 | エメラルド セラピューティクス,インコーポレーテッド | Information propagation system and method using modified nucleic acids |
AU2011319755B2 (en) | 2010-10-27 | 2017-02-23 | President And Fellows Of Harvard College | Compositions of toehold primer duplexes and methods of use |
US20140302486A1 (en) | 2011-09-02 | 2014-10-09 | President And Fellows Of Harvard College | Systems and methods for detecting biomarkers of interest |
JP6427753B2 (en) * | 2013-09-11 | 2018-11-28 | 国立大学法人大阪大学 | Thermal convection generating chip, thermal convection generating device, and thermal convection generating method |
CA3176365A1 (en) | 2013-12-16 | 2015-06-25 | William Marsh Rice University | Fine-tuned ultraspecific nucleic acid hybridization probes |
-
2017
- 2017-03-28 PT PT177764537T patent/PT3436198T/en unknown
- 2017-03-28 US US16/089,496 patent/US20200324292A1/en not_active Abandoned
- 2017-03-28 KR KR1020187030052A patent/KR102472071B1/en active IP Right Grant
- 2017-03-28 CA CA3019422A patent/CA3019422A1/en active Pending
- 2017-03-28 SG SG11201808493QA patent/SG11201808493QA/en unknown
- 2017-03-28 EP EP22156862.9A patent/EP4063014A1/en active Pending
- 2017-03-28 BR BR112018070197A patent/BR112018070197A2/en not_active Application Discontinuation
- 2017-03-28 JP JP2018551295A patent/JP6998319B2/en active Active
- 2017-03-28 KR KR1020227041286A patent/KR20230004792A/en not_active Application Discontinuation
- 2017-03-28 WO PCT/US2017/024530 patent/WO2017172760A1/en active Application Filing
- 2017-03-28 DK DK17776453.7T patent/DK3436198T3/en active
- 2017-03-28 EP EP17776453.7A patent/EP3436198B1/en active Active
- 2017-03-28 AU AU2017241766A patent/AU2017241766A1/en not_active Abandoned
- 2017-03-28 ES ES17776453T patent/ES2920954T3/en active Active
- 2017-03-28 RU RU2018137800A patent/RU2018137800A/en not_active Application Discontinuation
- 2017-03-28 CN CN201780026551.7A patent/CN109477136A/en active Pending
-
2018
- 2018-09-26 IL IL261980A patent/IL261980B2/en unknown
- 2018-09-28 PH PH12018502100A patent/PH12018502100A1/en unknown
-
2021
- 2021-12-20 JP JP2021205691A patent/JP2022037151A/en not_active Withdrawn
-
2023
- 2023-06-23 AU AU2023203972A patent/AU2023203972A1/en active Pending
- 2023-11-10 JP JP2023192003A patent/JP2023184748A/en active Pending
Also Published As
Publication number | Publication date |
---|---|
DK3436198T3 (en) | 2022-06-27 |
IL261980A (en) | 2018-10-31 |
IL261980B1 (en) | 2023-04-01 |
CA3019422A1 (en) | 2017-10-05 |
KR20230004792A (en) | 2023-01-06 |
AU2017241766A1 (en) | 2018-10-25 |
EP3436198A1 (en) | 2019-02-06 |
EP4063014A1 (en) | 2022-09-28 |
PT3436198T (en) | 2022-07-13 |
EP3436198A4 (en) | 2019-10-30 |
KR20190021198A (en) | 2019-03-05 |
WO2017172760A1 (en) | 2017-10-05 |
AU2023203972A1 (en) | 2023-07-13 |
IL261980B2 (en) | 2023-08-01 |
EP3436198B1 (en) | 2022-05-04 |
ES2920954T3 (en) | 2022-08-12 |
JP2023184748A (en) | 2023-12-28 |
RU2018137800A (en) | 2020-04-29 |
JP6998319B2 (en) | 2022-01-18 |
BR112018070197A2 (en) | 2019-01-29 |
RU2018137800A3 (en) | 2020-06-18 |
KR102472071B1 (en) | 2022-11-29 |
JP2019509751A (en) | 2019-04-11 |
PH12018502100A1 (en) | 2019-07-29 |
CN109477136A (en) | 2019-03-15 |
US20200324292A1 (en) | 2020-10-15 |
JP2022037151A (en) | 2022-03-08 |
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