SG11201804144UA - High-throughput imaging-based methods for predicting cell-type-specific toxicity of xenobiotics with diverse chemical structures - Google Patents
High-throughput imaging-based methods for predicting cell-type-specific toxicity of xenobiotics with diverse chemical structuresInfo
- Publication number
- SG11201804144UA SG11201804144UA SG11201804144UA SG11201804144UA SG11201804144UA SG 11201804144U A SG11201804144U A SG 11201804144UA SG 11201804144U A SG11201804144U A SG 11201804144UA SG 11201804144U A SG11201804144U A SG 11201804144UA SG 11201804144U A SG11201804144U A SG 11201804144UA
- Authority
- SG
- Singapore
- Prior art keywords
- singapore
- international
- biopolis
- institute
- cell
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 230000001988 toxicity Effects 0.000 title abstract 4
- 231100000419 toxicity Toxicity 0.000 title abstract 4
- 238000003384 imaging method Methods 0.000 title abstract 2
- 239000000126 substance Substances 0.000 title abstract 2
- 239000002676 xenobiotic agent Substances 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 abstract 4
- 150000001875 compounds Chemical class 0.000 abstract 3
- 239000011159 matrix material Substances 0.000 abstract 3
- 241000549556 Nanos Species 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 238000001727 in vivo Methods 0.000 abstract 2
- 102000007469 Actins Human genes 0.000 abstract 1
- 108010085238 Actins Proteins 0.000 abstract 1
- 238000004422 calculation algorithm Methods 0.000 abstract 1
- 210000004748 cultured cell Anatomy 0.000 abstract 1
- 231100000673 dose–response relationship Toxicity 0.000 abstract 1
- 210000003734 kidney Anatomy 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 238000010801 machine learning Methods 0.000 abstract 1
- 230000008520 organization Effects 0.000 abstract 1
- 230000002685 pulmonary effect Effects 0.000 abstract 1
- 238000007637 random forest analysis Methods 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/0002—Inspection of images, e.g. flaw detection
- G06T7/0012—Biomedical image inspection
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/10—Segmentation; Edge detection
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/10—Image acquisition modality
- G06T2207/10056—Microscopic image
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/10—Image acquisition modality
- G06T2207/10064—Fluorescence image
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/30—Subject of image; Context of image processing
- G06T2207/30004—Biomedical image processing
- G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Theoretical Computer Science (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Toxicology (AREA)
- Biotechnology (AREA)
- Medical Informatics (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- Radiology & Medical Imaging (AREA)
- Quality & Reliability (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Computational Biology (AREA)
- Evolutionary Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) CORRECTED VERSION (19) World Intellectual Property Organization International Bureau .9 . si ww..0.7,..d ei (43) International Publication Date issor 1 June 2017 (01.06.2017) WIPO I PCT ID Hit (10) WO International 2017/091147 111111111111111111111111111111111111111111111111111111111111111t111111111111111 Publication A8 Number (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every G01N 33/50 (2006.01) G06T 7/00 (2017.01) kind of national protection available): AE, AG, AL, AM, G06F 19/00 (2011.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, (21) International Application Number: DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, PCT/SG2016/050554 HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, (22) International Filing Date: KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, 9 November 2016 (09.11.2016) MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (25) Filing Language: English SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, (26) Publication Language: English TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: 10201509598X 20 November 2015 (20.11.2015) SG (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, Connexis, Singapore 138632 (SG). TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, (72) Inventors: LOO, Lit-Hsin; c/o Bioinformatics Institute, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, 30 Biopolis St, Matrix, #07-01, Singapore 138671 (SG). TR), BJ, SM, OAPI (BF, CF, CG, CI, CM, GA, GN, GQ, LEE, Jia Ying; c/o Bioinfoiinatics Institute, 30 Biopolis GW, KM, ML, MR, NE, SN, TD, TG). St, Matrix, #07-01, Singapore 138671 (SG). SU, Ran; c/o Bioinfoiinatics Institute, 30 Biopolis St, Matrix, #07-01, Published: Singapore 138671 (SG). ZINK, Daniele; c/o Institute of with international search report (Art 21(3)) Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, #04-01, Singapore 138669 (SG). XIONG, (48) Date of publication of this corrected version: Sijing; c/o Institute of Bioengineering and Nanotechno- 29 June 2017 logy, 31 Biopolis Way, The Nanos, #04-01, Singapore (15) Information about Correction: 138669 (SG). see Notice of 29 June 2017 Agent: MATTEUCCI, Gianfranco; Marks & Clerk (74) Singapore LLP, Tanjong Pagar, P.O. Box 636, Singapore 910816 (SG). GC .4 t IN it 1-1 (54) Title: HIGH-THROUGHPUT IMAGING-BASED METHODS FOR PREDICTING CELL-TYPE-SPECIFIC TOXICITY OF Il cp \ XENOBIOTICS WITH DIVERSE CHEMICAL STRUCTURES 0 ---- (57) : The present invention provides methods for the prediction of in vivo cell-specific toxicity of a compound that com - I N biases high-throughput imaging of cultured cells, quantitative phenotypic profiling, and machine learning methods. More particularly, Il c:::, the invention provides a method for the prediction of in vivo renal proximal tubular-, bronchial-epithelial-, and alveolar-cell-specific N toxicities of a soluble or particulate compound that comprises contacting cultured human kidney and pulmonary cells with the com- 0 pound at a range of concentrations, then labelling the cells with DNA, yH2AX and actin markers and obtaining textural features, spatial correlation features, ratios of the markers, intensity features, cell count and morphology, estimating dose response curves and performing automatic classification of the compound using a random-forest algorithm.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SG10201509598X | 2015-11-20 | ||
| PCT/SG2016/050554 WO2017091147A1 (en) | 2015-11-20 | 2016-11-09 | High-throughput imaging-based methods for predicting cell-type-specific toxicity of xenobiotics with diverse chemical structures |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201804144UA true SG11201804144UA (en) | 2018-06-28 |
Family
ID=58764375
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201804144UA SG11201804144UA (en) | 2015-11-20 | 2016-11-09 | High-throughput imaging-based methods for predicting cell-type-specific toxicity of xenobiotics with diverse chemical structures |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20200309767A1 (en) |
| EP (1) | EP3377896A4 (en) |
| KR (1) | KR20180086438A (en) |
| CN (1) | CN108885204B (en) |
| SG (1) | SG11201804144UA (en) |
| WO (1) | WO2017091147A1 (en) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108388860B (en) * | 2018-02-12 | 2020-04-28 | 大连理工大学 | Aero-engine rolling bearing fault diagnosis method based on power entropy spectrum-random forest |
| JP6558786B1 (en) * | 2018-09-28 | 2019-08-14 | 学校法人東北工業大学 | Method, computer system, and program for predicting target characteristics |
| EP3928250A1 (en) | 2019-02-20 | 2021-12-29 | Bluerock Therapeutics LP | Detecting cells of interest in large image datasets using artificial intelligence |
| CN110992320B (en) * | 2019-11-22 | 2023-03-21 | 电子科技大学 | Medical image segmentation network based on double interleaving |
| KR102369717B1 (en) * | 2019-12-19 | 2022-03-03 | 인제대학교 산학협력단 | Multi-features classification of prostate carcinoma observed in histological sections |
| CN113192553B (en) * | 2020-01-14 | 2022-09-09 | 北京大学 | Method for predicting cell spatial relationship based on single cell transcriptome sequencing data |
| CN111751263A (en) * | 2020-06-08 | 2020-10-09 | 西安交通大学 | Mark-free cell two-dimensional scattering image inversion method based on gray level co-occurrence matrix |
| CN112395747B (en) * | 2020-11-04 | 2023-05-09 | 南京信息工程大学 | Method for predicting toxicity of mixed pollutants in complex combined action mode |
| CN115142160B (en) * | 2022-08-22 | 2023-12-19 | 无锡物联网创新中心有限公司 | Identification method and related device for strong weak ring of yarn |
| US11874934B1 (en) | 2023-01-19 | 2024-01-16 | Citibank, N.A. | Providing user-induced variable identification of end-to-end computing system security impact information systems and methods |
| US11748491B1 (en) * | 2023-01-19 | 2023-09-05 | Citibank, N.A. | Determining platform-specific end-to-end security vulnerabilities for a software application via a graphical user interface (GUI) systems and methods |
| CN116595427B (en) * | 2023-07-18 | 2023-09-19 | 青岛海关技术中心 | Chemical potential long-term health hazard classification prediction method, medium and system |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003286697A1 (en) * | 2002-10-25 | 2004-05-13 | Norak Biosciences, Inc. | Methods of identifying reduced internalization transmembrane receptor agonists |
| GB2435923A (en) * | 2006-03-09 | 2007-09-12 | Cytokinetics Inc | Cellular predictive models for toxicities |
| GB2435925A (en) * | 2006-03-09 | 2007-09-12 | Cytokinetics Inc | Cellular predictive models for toxicities |
| US20080195322A1 (en) * | 2007-02-12 | 2008-08-14 | The Board Of Regents Of The University Of Texas System | Quantification of the Effects of Perturbations on Biological Samples |
| US8818070B2 (en) * | 2011-02-28 | 2014-08-26 | Cellomics, Inc. | Predicting toxicity of a compound over a range of concentrations |
| DK2773955T3 (en) * | 2011-11-04 | 2018-09-24 | Inregen | PHARMACEUTICAL SCREENING AND STRENGTH ASSAYS |
| WO2014013329A1 (en) * | 2012-07-20 | 2014-01-23 | Agency For Science, Technology And Research | In vitro assay for predicting renal proximal tubular cell toxicity |
| JP6491671B2 (en) * | 2014-03-17 | 2019-03-27 | エージェンシー フォー サイエンス， テクノロジー アンド リサーチ | Method for predicting compound toxicity based on nuclear factor-κB translocation |
-
2016
- 2016-11-09 WO PCT/SG2016/050554 patent/WO2017091147A1/en active Application Filing
- 2016-11-09 EP EP16868991.7A patent/EP3377896A4/en not_active Withdrawn
- 2016-11-09 SG SG11201804144UA patent/SG11201804144UA/en unknown
- 2016-11-09 CN CN201680079245.5A patent/CN108885204B/en not_active Expired - Fee Related
- 2016-11-09 KR KR1020187017042A patent/KR20180086438A/en not_active Application Discontinuation
- 2016-11-09 US US15/777,653 patent/US20200309767A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP3377896A4 (en) | 2019-04-10 |
| KR20180086438A (en) | 2018-07-31 |
| CN108885204A (en) | 2018-11-23 |
| EP3377896A1 (en) | 2018-09-26 |
| WO2017091147A8 (en) | 2017-06-29 |
| WO2017091147A1 (en) | 2017-06-01 |
| US20200309767A1 (en) | 2020-10-01 |
| CN108885204B (en) | 2021-10-26 |
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