SG11201910195WA - Methods for trapping and barcoding discrete biological units in hydrogel - Google Patents

Methods for trapping and barcoding discrete biological units in hydrogel

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Publication number
SG11201910195WA
SG11201910195WA SG11201910195WA SG11201910195WA SG 11201910195W A SG11201910195W A SG 11201910195WA SG 11201910195W A SG11201910195W A SG 11201910195WA SG 11201910195W A SG11201910195W A SG 11201910195WA
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Singapore
Prior art keywords
fr
international
methods
biological units
barcoding
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Inventor
Stuart J Edelstein
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Scipio Bioscience
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Publication date
Priority to US201762502180P priority Critical
Application filed by Scipio Bioscience filed Critical Scipio Bioscience
Priority to PCT/IB2018/000612 priority patent/WO2018203141A1/en
Publication of SG11201910195WA publication Critical patent/SG11201910195WA/en

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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6903Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being semi-solid, e.g. an ointment, a gel, a hydrogel or a solidifying gel
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q2547/00Reactions characterised by the features used to prevent contamination
    • C12Q2547/10Reactions characterised by the features used to prevent contamination the purpose being preventing contamination
    • C12Q2547/107Use of permeable barriers, e.g. waxes
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    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149Particles, e.g. beads
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    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
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    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
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    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 08 November 2018 (08.11.2018) WIPO I PCT omit VIII °nolo H01°1111111 olowliflo oimIE (10) International Publication Number WO 2018/203141 Al (51) International Patent Classification: C12Q 1/6806 (2018.01) Cl 2N 5/00 (2006.01) C12Q 1/6874 (2018.01) (21) International Application Number: PCT/IB2018/000612 (22) International Filing Date: 04 May 2018 (04.05.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/502,180 05 May 2017 (05.05.2017) US (71) Applicant: SCIPIO BIOSCIENCE [FR/FR]; 17 rue de la Vanne, 92120 Montrouge (FR). (72) Inventor: EDELSTEIN, Stuart J.; 94 rue Montmartre, 75002 Paris (FR). (74) Agent: ICOSA; 83 avenue Denfert-Rochereau, 75014 Paris (FR). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: of inventorship (Rule 4.17(iv)) Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) 1-1 O 71' 1-1 ! (54) Title: METHODS FOR TRAPPING AND BARCODING DISCRETE BIOLOGICAL UNITS IN HYDROGEL (57) : The present invention relates to methods for trapping and barcoding discrete biological units in a hydrogel. It further 0 relates to methods for analyzing gene expression, genotype, haplotype or epigenome in discrete biological units. The present invention \" also relates to kits for implementing the methods of the present disclosure.
SG11201910195W 2017-05-05 2018-05-04 Methods for trapping and barcoding discrete biological units in hydrogel SG11201910195WA (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US201762502180P true 2017-05-05 2017-05-05
PCT/IB2018/000612 WO2018203141A1 (en) 2017-05-05 2018-05-04 Methods for trapping and barcoding discrete biological units in hydrogel

Publications (1)

Publication Number Publication Date
SG11201910195WA true SG11201910195WA (en) 2019-11-28

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SG11201910195W SG11201910195WA (en) 2017-05-05 2018-05-04 Methods for trapping and barcoding discrete biological units in hydrogel

Country Status (8)

Country Link
US (1) US20180320173A1 (en)
EP (1) EP3619325A1 (en)
KR (1) KR20200004335A (en)
AU (1) AU2018262331A1 (en)
CA (1) CA3062248A1 (en)
GB (1) GB2577214A (en)
SG (1) SG11201910195WA (en)
WO (1) WO2018203141A1 (en)

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WO2005003304A2 (en) 2003-06-20 2005-01-13 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
AU2013359165B2 (en) 2012-12-14 2019-09-12 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20150119047A (en) 2013-02-08 2015-10-23 10엑스 제노믹스, 인크. Polynucleotide barcode generation
CN105339503A (en) 2013-05-23 2016-02-17 斯坦福大学托管董事会 Transposition into native chromatin for personal epigenomics
GB2525104B (en) 2013-08-28 2016-09-28 Cellular Res Inc Massively Parallel Single Cell Nucleic Acid Analysis
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
JP6366719B2 (en) 2013-12-20 2018-08-01 イルミナ インコーポレイテッド Preservation of genomic connectivity information in fragmented genomic DNA samples
US10422002B2 (en) 2014-02-18 2019-09-24 Illumina, Inc. Methods and compositions for DNA profiling
US20160060621A1 (en) 2014-06-24 2016-03-03 Bio-Rad Laboratories, Inc. Digital pcr barcoding
EP3161162A4 (en) 2014-06-26 2018-01-10 10X Genomics, Inc. Analysis of nucleic acid sequences
JP2017522867A (en) 2014-06-26 2017-08-17 10エックス ジェノミクス, インコーポレイテッド Methods for analyzing nucleic acids from individual cells or cell populations
EP3161152B1 (en) 2014-06-30 2018-12-26 Illumina, Inc. Methods and compositions using one-sided transposition
JP2018501776A (en) 2014-10-17 2018-01-25 イルミナ ケンブリッジ リミテッド Dislocations that maintain continuity
CN107407691A (en) * 2015-01-22 2017-11-28 贝克顿迪金森公司 Device and system for the molecular bar code of unicellular amplifying nucleic acid target
BR112017017025A2 (en) 2015-02-10 2018-04-10 Illumina Inc methods and compositions for analyzing cellular components
WO2017075265A1 (en) * 2015-10-28 2017-05-04 The Broad Institute, Inc. Multiplex analysis of single cell constituents

Also Published As

Publication number Publication date
AU2018262331A8 (en) 2019-12-12
KR20200004335A (en) 2020-01-13
GB2577214A (en) 2020-03-18
US20180320173A1 (en) 2018-11-08
CA3062248A1 (en) 2018-11-08
AU2018262331A1 (en) 2019-11-21
EP3619325A1 (en) 2020-03-11
GB201917721D0 (en) 2020-01-15
WO2018203141A1 (en) 2018-11-08

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