SG11201804401VA - Determination of nucleic acid methylation - Google Patents
Determination of nucleic acid methylationInfo
- Publication number
- SG11201804401VA SG11201804401VA SG11201804401VA SG11201804401VA SG11201804401VA SG 11201804401V A SG11201804401V A SG 11201804401VA SG 11201804401V A SG11201804401V A SG 11201804401VA SG 11201804401V A SG11201804401V A SG 11201804401VA SG 11201804401V A SG11201804401V A SG 11201804401VA
- Authority
- SG
- Singapore
- Prior art keywords
- international
- methylation
- nucleic acid
- target
- singapore
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2523/00—Reactions characterised by treatment of reaction samples
- C12Q2523/10—Characterised by chemical treatment
- C12Q2523/125—Bisulfite(s)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/113—Modifications characterised by incorporating modified backbone
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/131—Allele specific probes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/163—Reactions characterised by the reaction format or use of a specific feature the purpose or use of blocking probe
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/155—Particles of a defined size, e.g. nanoparticles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/107—Alteration in the property of hybridised versus free label oligonucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Nanotechnology (AREA)
- General Chemical & Material Sciences (AREA)
Abstract
INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property ' Organization International Bureau res.. 00) (43) International Publication Date .... ...r .„„*./ 1 June 2017 (01.06.2017) WIPO I PCT (10) WO International 111111111111311111111111111111111111111111111111111111111111111111111311111111111111111 2017/091156 Publication Number Al (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12Q 1/68 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (21) International Application Number: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, PCT/SG2016/050583 DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (22) International Filing Date: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, 25 November 2016 (25.11.2016) KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (25) Filing Language: English OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (26) Publication Language: English SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, (30) Priority Data: ZW. 10201509792S 27 November 2015 (27.11.2015) SG (84) Designated States (unless otherwise indicated, for every (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY kind of regional protection available): ARIPO (BW, GH, AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, Connexis, Singapore 138632 (SG). TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (72) Inventors: ZU, Yanbing; do Institute of Bioengineering TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, and Nanotechnology, 31 Biopolis Way, The Nanos, #04- 01, Singapore 138669 (SG). YING, Jackie Y.; c/o Institute LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, of Bioengineering and Nanotechnology, 31 Biopolis Way, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). The Nanos, #04-01, Singapore 138669 (SG). Published: (74) Agent: VIERING, JENTSCHURA & PARTNER LLP; 21(3)) P.O. Box 1088, Rochor Post Office, Rochor Road, Singa- — with international search report (Art pore 911833 (SG). — with sequence listing part of description (Rule 5.2(a)) (54) Title: DETERMINATION OF NUCLEIC ACID METHYLATION Figure 1 \ lix yi s 'Iml kelp D 11 A.mt3lify .. `iEPT., \ Read A co, i Isasst s Lit A Jn AS 6.Ieekee p:pm:Pter PCP Futiuct Various Promega Modified Asymmetric IEN nanoprobe Color signals o commercial MethylEdgeT\" sequences PCR methods Bisulfite according to Conversion references ( - MS methylation-specific) System - 2 5 h t 2. , t mitt V: in 1 Li min IS :. <,‘,.. ‘ CIr.\" If , I :\"' \".: \" ' 3,wkau*nmlis-, 11 os Cr\ i3,:n.st(sft {flati tleikbti.,${ .\.... L ....' (57) : The invention relates to methods and ..::::; sample comprising (a) bisulfite treatment of the target el tion-specific primers, (c) contacting the amplicons Oation status based on melting temperature T„, of the ,-t particle covalently coupled to a morpholino oligonucleotide . kits for determining the methylation nucleic acid, (b) amplifying with a methylation-specific plasmonic hybrid probe and the target probe. Also claimed - the nucleic are methods Mr.;$ 141a/ m, status of a target treated target nucleic nanoprobe and (d) acid. In particular, and kits for nucleic acid molecule in a acid with methyla - deteiinining the methyl - a plasmonic gold nano - determining methylation status of the Septin 9 (SEPT9) gene promoter.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SG10201509792S | 2015-11-27 | ||
PCT/SG2016/050583 WO2017091156A1 (en) | 2015-11-27 | 2016-11-25 | Determination of nucleic acid methylation |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11201804401VA true SG11201804401VA (en) | 2018-06-28 |
Family
ID=58764356
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG10201912967YA SG10201912967YA (en) | 2015-11-27 | 2016-11-25 | Determination of nucleic acid methylation |
SG11201804401VA SG11201804401VA (en) | 2015-11-27 | 2016-11-25 | Determination of nucleic acid methylation |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG10201912967YA SG10201912967YA (en) | 2015-11-27 | 2016-11-25 | Determination of nucleic acid methylation |
Country Status (5)
Country | Link |
---|---|
US (2) | US11021743B2 (en) |
EP (1) | EP3380637B1 (en) |
CN (1) | CN108495937A (en) |
SG (2) | SG10201912967YA (en) |
WO (1) | WO2017091156A1 (en) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP7245164B2 (en) * | 2017-08-31 | 2023-03-23 | 公益財団法人がん研究会 | Highly sensitive and quantitative genetic testing method, primer set, and test kit |
CN108796080B (en) * | 2018-06-06 | 2022-06-07 | 苏州唯善生物科技有限公司 | Primer and probe set for colorectal cancer diagnosis, detection or screening |
CN109929919B (en) * | 2018-09-14 | 2020-02-14 | 深圳市晋百慧生物有限公司 | DNA methylation detection method and related application |
CN115786517A (en) * | 2019-02-14 | 2023-03-14 | 博尔诚(北京)科技有限公司 | Composition for screening cancers of intestinal cancers and precancers and application of composition |
CN109765371A (en) * | 2019-02-19 | 2019-05-17 | 吉林省爱诺德生物工程有限公司 | A kind of liquid phase nano colloid gold colorimetric method of quick detection neoplasm dna |
MX2022007434A (en) * | 2019-12-16 | 2022-09-19 | Epigenomics Ag | Methods for detecting colorectal cancer. |
CN112195243A (en) * | 2020-09-22 | 2021-01-08 | 北京华大吉比爱生物技术有限公司 | Kit for detecting polygene methylation and application thereof |
CN116949156B (en) * | 2023-09-19 | 2023-12-08 | 美迪西普亚医药科技(上海)有限公司 | Analysis method for detecting human T cells in general way based on nucleic acid variants |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5472881A (en) | 1992-11-12 | 1995-12-05 | University Of Utah Research Foundation | Thiol labeling of DNA for attachment to gold surfaces |
WO2003048769A1 (en) * | 2001-11-30 | 2003-06-12 | Nanosphere, Inc. | Real-time monitoring of pcr amplification using nanoparticle probes |
US20080241828A1 (en) * | 2007-03-30 | 2008-10-02 | Kai Wu | Detection of dna methylation using raman spectroscopy |
EP3101141B1 (en) * | 2007-06-08 | 2019-10-16 | Epigenomics AG | Method for methylation analysis |
EP2189538A1 (en) * | 2008-11-21 | 2010-05-26 | Epigenomics AG | An analysis molecule and method for the analysis of a nucleotide position in a nucleic acid |
CN102884204B (en) * | 2010-01-12 | 2015-03-25 | 新加坡科技研究局 | Sequence-selective recognition of nucleic acids using nanoparticle probes |
WO2014149356A1 (en) * | 2013-03-15 | 2014-09-25 | Abbott Molecular Inc. | Detection of bisulfite converted nucleotide sequences |
SG10201708046YA (en) * | 2013-04-11 | 2017-10-30 | Agency Science Tech & Res | Nanoprobe-Based Genetic Testing |
CN105722999A (en) * | 2013-09-17 | 2016-06-29 | 新加坡科技研究局 | Method for detection of a genetic variant |
CN103789429B (en) * | 2014-01-24 | 2015-12-30 | 武汉大学 | A kind of method of the 5-methylcytosine detected in DNA |
KR101612885B1 (en) * | 2014-03-27 | 2016-04-26 | 고려대학교 산학협력단 | Methods for Detection Methylation on Promoter and Methods for Detection and Tracking Epigenetics Alterations by DNA methylation Using Nanoplasmonic Biosensor |
-
2016
- 2016-11-25 SG SG10201912967YA patent/SG10201912967YA/en unknown
- 2016-11-25 CN CN201680079795.7A patent/CN108495937A/en active Pending
- 2016-11-25 SG SG11201804401VA patent/SG11201804401VA/en unknown
- 2016-11-25 US US15/778,876 patent/US11021743B2/en active Active
- 2016-11-25 EP EP16868998.2A patent/EP3380637B1/en active Active
- 2016-11-25 WO PCT/SG2016/050583 patent/WO2017091156A1/en active Application Filing
-
2021
- 2021-04-01 US US17/220,658 patent/US20210222237A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
WO2017091156A1 (en) | 2017-06-01 |
SG10201912967YA (en) | 2020-02-27 |
CN108495937A (en) | 2018-09-04 |
EP3380637B1 (en) | 2020-09-30 |
US11021743B2 (en) | 2021-06-01 |
EP3380637A1 (en) | 2018-10-03 |
EP3380637A4 (en) | 2019-05-01 |
US20190017110A1 (en) | 2019-01-17 |
US20210222237A1 (en) | 2021-07-22 |
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