SG11201906553TA - Methods and reagents for synthesising polynucleotide molecules - Google Patents

Methods and reagents for synthesising polynucleotide molecules

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Publication number
SG11201906553TA
SG11201906553TA SG11201906553TA SG11201906553TA SG11201906553TA SG 11201906553T A SG11201906553T A SG 11201906553TA SG 11201906553T A SG11201906553T A SG 11201906553TA SG 11201906553T A SG11201906553T A SG 11201906553TA SG 11201906553T A SG11201906553T A SG 11201906553TA
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Singapore
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international
village
suite
science
methods
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SG11201906553TA
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John Milton
Sobia Nayyar
Jan Riedl
Ryosuke Ogaki
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Oxford Nanopore Tech Ltd
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Publication of SG11201906553TA publication Critical patent/SG11201906553TA/en

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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12N15/09—Recombinant DNA-technology
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    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
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    • B01J2219/00718—Type of compounds synthesised
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    • B01J2219/00722—Nucleotides
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
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    • C12Q2521/531—Glycosylase
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/301—Hairpin oligonucleotides
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    • C12Q2537/162—Helper probe
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Abstract

VoodooI Method . Depcoixetlon (106,110) 1. Provision of scaffold two b Un 2. Incorporation (102, 107) w UnX wow s Support strand bls tossarksior gm* b Synthesis skied (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 26 July 2018 (26.07.2018) WIP0 I PCT omit IIl °nolo Oil IIII 1111101 ois (10) International Publication Number WO 2018/134616 Al (51) International Patent Classification: BO1J 19/00 (2006.01) C12Q 1/6844 (2018.01) (21) International Application Number: PCT/GB2018/050165 (22) International Filing Date: 19 January 2018 (19.01.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 1700937.4 19 January 2017 (19.01.2017) GB (71) Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED [GB/GB]; Suite 14 Science Village, Chester- ford Research Park, Cambridge Cambridgeshire CB10 1XL (GB). (72) Inventors: MILTON, John; Oxford Nanopore Technolo- gies Ltd., Suite 14 Science Village, Chesterford Research Park, Cambridge Cambridgeshire CB10 1XL (GB). NAY- YAR, Sobia; Oxford Nanopore Technologies Ltd., Suite 14 Science Village, Chesterford Research Park, Cambridge Cambridgeshire CB10 1XL (GB). RIEDL, Jan; Oxford Nanopore Technologies Ltd., Suite 14 Science Village, Chesterford Research Park, Cambridge Cambridgeshire CB10 1XL (GB). OGAKI, Ryosuke; Oxford Nanopore Technologies Ltd., Suite 14 Science Village, Chesterford Research Park, Cambridge Cambridgeshire CB10 1XL (GB). (74) Agent: WILKINSON, Marc George; J A Kemp, 14 South Square, Gray's Inn, London Greater London WC1R 5JJ (GB). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (54) Title: METHODS AND REAGENTS FOR SYNTHESISING POLYNUCLEOTIDE MOLECULES (57) : The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide 0 sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis, as well as systems \" and kits for performing the synthesis and/or assembly methods. C [Continued on next page] WO 2018/134616 Al MIDEDIMOMOIDEIREEMOMMIONHOI111111111110111111111111 GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a))
SG11201906553TA 2017-01-19 2018-01-19 Methods and reagents for synthesising polynucleotide molecules SG11201906553TA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1700937.4A GB2559117B (en) 2017-01-19 2017-01-19 Double stranded polynucleotide synthesis method, kit and system
PCT/GB2018/050165 WO2018134616A1 (en) 2017-01-19 2018-01-19 Methods and reagents for synthesising polynucleotide molecules

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US (2) US10927394B2 (en)
EP (2) EP3570972B1 (en)
JP (1) JP7295021B2 (en)
KR (1) KR102618717B1 (en)
CN (1) CN110461463B (en)
AU (1) AU2018209365B2 (en)
CA (1) CA3050822C (en)
GB (1) GB2559117B (en)
SG (1) SG11201906553TA (en)
WO (1) WO2018134616A1 (en)

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GB201811811D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
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