RU2018137800A - Обнаружение нуклеиновых кислот на поверхности в устройствах с конвекционным потоком жидкости - Google Patents

Обнаружение нуклеиновых кислот на поверхности в устройствах с конвекционным потоком жидкости Download PDF

Info

Publication number
RU2018137800A
RU2018137800A RU2018137800A RU2018137800A RU2018137800A RU 2018137800 A RU2018137800 A RU 2018137800A RU 2018137800 A RU2018137800 A RU 2018137800A RU 2018137800 A RU2018137800 A RU 2018137800A RU 2018137800 A RU2018137800 A RU 2018137800A
Authority
RU
Russia
Prior art keywords
reaction chamber
oligonucleotide
sequence
liquid reaction
nucleotides
Prior art date
Application number
RU2018137800A
Other languages
English (en)
Russian (ru)
Other versions
RU2018137800A3 (enExample
Inventor
Дмитрий А. ХОДАКОВ
Дэвид ЧЖАН
Original Assignee
Уильям Марш Райс Юниверсити
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Уильям Марш Райс Юниверсити filed Critical Уильям Марш Райс Юниверсити
Publication of RU2018137800A publication Critical patent/RU2018137800A/ru
Publication of RU2018137800A3 publication Critical patent/RU2018137800A3/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
RU2018137800A 2016-03-29 2017-03-28 Обнаружение нуклеиновых кислот на поверхности в устройствах с конвекционным потоком жидкости RU2018137800A (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662314909P 2016-03-29 2016-03-29
US62/314,909 2016-03-29
PCT/US2017/024530 WO2017172760A1 (en) 2016-03-29 2017-03-28 Surface-based detection of nucleic acid in a convection flow fluidic device

Publications (2)

Publication Number Publication Date
RU2018137800A true RU2018137800A (ru) 2020-04-29
RU2018137800A3 RU2018137800A3 (enExample) 2020-06-18

Family

ID=59965134

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2018137800A RU2018137800A (ru) 2016-03-29 2017-03-28 Обнаружение нуклеиновых кислот на поверхности в устройствах с конвекционным потоком жидкости

Country Status (15)

Country Link
US (2) US20200324292A1 (enExample)
EP (2) EP4063014A1 (enExample)
JP (3) JP6998319B2 (enExample)
KR (2) KR102472071B1 (enExample)
CN (1) CN109477136A (enExample)
AU (2) AU2017241766A1 (enExample)
BR (1) BR112018070197A2 (enExample)
DK (1) DK3436198T3 (enExample)
ES (1) ES2920954T3 (enExample)
IL (1) IL261980B2 (enExample)
PH (1) PH12018502100A1 (enExample)
PT (1) PT3436198T (enExample)
RU (1) RU2018137800A (enExample)
SG (1) SG11201808493QA (enExample)
WO (1) WO2017172760A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12599908B2 (en) * 2018-09-14 2026-04-14 William Marsh Rice University Apparatus and methods for multiplexed amplification and detection of DNA using convectional heating and label-free microarray
CN113257351A (zh) * 2020-02-12 2021-08-13 赛纳生物科技(北京)有限公司 一种用于多碱基基因测序的基因文库及其构建方法
CN111879922A (zh) * 2020-07-13 2020-11-03 东南大学 适用于核酸及免疫同步检测集成纸基芯片结构及制造方法
DE102020212253A1 (de) * 2020-09-29 2022-03-31 SpinDiag GmbH Probenträger und Rotationsvorrichtung
EP4640315A1 (en) * 2024-04-26 2025-10-29 Appolon Bioteck Devices and methods for rapid nucleic acid amplification

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027880A (en) 1995-08-02 2000-02-22 Affymetrix, Inc. Arrays of nucleic acid probes and methods of using the same for detecting cystic fibrosis
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US6642000B1 (en) 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides
JP2003522963A (ja) * 2000-02-18 2003-07-29 アクララ バイオサイエンシーズ， インコーポレイテッド 複数部位反応デバイスおよび方法
US6586233B2 (en) 2001-03-09 2003-07-01 The Regents Of The University Of California Convectively driven PCR thermal-cycling
RU2216547C2 (ru) 2001-10-16 2003-11-20 Институт молекулярной биологии им. В.А. Энгельгардта РАН Способ полимеризационной иммобилизации биологических макромолекул и композиция для его осуществления
WO2004033728A2 (en) * 2002-10-11 2004-04-22 Erasmus Universiteit Rotterdam Nucleic acid amplification primers for pcr-based clonality studies
EP1616947B1 (en) * 2003-03-28 2012-05-09 Japan as represented by Director General of National Rehabilitation Center for Persons with Disabilities METHOD OF SYNTHESIZING cDNA
KR100647289B1 (ko) * 2004-09-15 2006-11-23 삼성전자주식회사 마랑고니 대류를 이용한 ｐｃｒ 장치 및 이를 이용한ｐｃｒ 방법
US20060223122A1 (en) * 2005-03-08 2006-10-05 Agnes Fogo Classifying and predicting glomerulosclerosis using a proteomics approach
GB2424946A (en) * 2005-04-05 2006-10-11 Stratec Biomedical Systems Ag A detection system for substance binding using up-converting fluorescent probes
EP1943348B1 (en) * 2005-10-03 2013-01-02 Life Technologies Corporation Compositions, methods, and kits for amplifying nucleic acids
US8114636B2 (en) * 2006-02-10 2012-02-14 Life Technologies Corporation Labeling and detection of nucleic acids
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
US8735103B2 (en) 2006-12-05 2014-05-27 Electronics And Telecommunications Research Institute Natural convection-driven PCR apparatus and method using disposable polymer chip
US8110353B2 (en) 2007-02-05 2012-02-07 California Institute Of Technology Engineered toehold reactions and networks
US8043810B2 (en) 2007-05-02 2011-10-25 Eagle Eye Research, Inc. Analyte detection using autocatalytic chain reactions
EP2610007B1 (en) 2007-07-23 2018-12-05 CLONDIAG GmbH Assays
CN103805507B (zh) 2008-01-24 2016-04-13 基亚生物科技股份有限公司 热对流聚合酶连锁反应之方法及装置
KR20100025328A (ko) * 2008-08-27 2010-03-09 삼성전자주식회사 이중가닥 영역과 말단 단일가닥 영역을 포함하는 이중가닥 핵산 프로브가 고정된 마이크로어레이를 제조하는 방법
CN102725424B (zh) * 2010-01-25 2014-07-09 Rd生物科技公司 靶核酸的自折叠扩增
WO2011097424A1 (en) * 2010-02-05 2011-08-11 Siemens Healthcare Diagnostics Inc. Methods for increasing multiplex level by externalization of passive reference in polymerase chain reactions
EP2363502B1 (en) * 2010-03-04 2017-02-15 miacom Diagnostics GmbH Enhanced multiplex FISH
EP2577534A4 (en) * 2010-05-27 2017-04-26 Emerald Therapeutics, Inc. System and method for propagating information using modified nucleic acids
CN103328654B (zh) 2010-10-27 2017-07-11 哈佛学院院长等 立足点引物双链体的组合物及其使用方法
WO2012162161A1 (en) * 2011-05-20 2012-11-29 Phthisis Diagnostics Microsporidia detection system and method
US20140302486A1 (en) 2011-09-02 2014-10-09 President And Fellows Of Harvard College Systems and methods for detecting biomarkers of interest
JP6427753B2 (ja) * 2013-09-11 2018-11-28 国立大学法人大阪大学 熱対流生成用チップ、熱対流生成装置、及び熱対流生成方法
ES2771773T3 (es) 2013-12-16 2020-07-07 Univ Rice William M Sondas de hibridación de ácido nucleico ultraespecíficas de ajuste fino
CN108463560B (zh) * 2016-01-15 2022-09-20 昆塔波尔公司 具有降低的背景的基于光学的纳米孔分析

Also Published As

Publication number Publication date
US20200324292A1 (en) 2020-10-15
US20240278249A1 (en) 2024-08-22
KR20190021198A (ko) 2019-03-05
JP2022037151A (ja) 2022-03-08
RU2018137800A3 (enExample) 2020-06-18
EP3436198A4 (en) 2019-10-30
PT3436198T (pt) 2022-07-13
PH12018502100A1 (en) 2019-07-29
JP6998319B2 (ja) 2022-01-18
IL261980A (en) 2018-10-31
CA3019422A1 (en) 2017-10-05
BR112018070197A2 (pt) 2019-01-29
EP3436198B1 (en) 2022-05-04
JP2019509751A (ja) 2019-04-11
EP4063014A1 (en) 2022-09-28
IL261980B1 (en) 2023-04-01
JP2023184748A (ja) 2023-12-28
AU2023203972A1 (en) 2023-07-13
ES2920954T3 (es) 2022-08-12
SG11201808493QA (en) 2018-10-30
KR102472071B1 (ko) 2022-11-29
AU2017241766A1 (en) 2018-10-25
WO2017172760A1 (en) 2017-10-05
KR20230004792A (ko) 2023-01-06
KR102789837B1 (ko) 2025-04-01
IL261980B2 (en) 2023-08-01
CN109477136A (zh) 2019-03-15
DK3436198T3 (en) 2022-06-27
EP3436198A1 (en) 2019-02-06

Similar Documents

Publication Publication Date Title
Yuan et al. Picoinjection-enabled multitarget loop-mediated isothermal amplification for detection of foodborne pathogens
Atceken et al. Point‐of‐care diagnostic platforms for loop‐mediated isothermal amplification
JP5731066B2 (ja) 核酸のための周期変動性増幅反応
US6303343B1 (en) Inefficient fast PCR
CA2384528C (en) Reaction system for performing in the amplification of nucleic acids
CN102625850A (zh) 多重核酸检测方法和系统
Shu et al. Segmented continuous-flow multiplex polymerase chain reaction microfluidics for high-throughput and rapid foodborne pathogen detection
Tröger et al. Isothermal amplification and quantification of nucleic acids and its use in microsystems
Zhang et al. Nanolitre droplet array for real time reverse transcription polymerase chain reaction
CN102428189A (zh) 反应筒内多重核酸序列的检测
EP3006938B1 (en) Real time quantitative and qualitative analysis method for biosubstance
JP2019509751A5 (enExample)
Dey Polymerase chain reaction: Principle, technique and applications in pathology
IL261980B2 (en) Surface-based detection of nucleic acids in convection flow fluidic devices
US20200063189A1 (en) System and method for purifying and amplifying nucleic acids
Guo et al. An integrated microfluidic chip for the detection of bacteria–A proof of concept
AU2011315926A1 (en) Quantitative multiplexed identification of nucleic acid targets
Wang et al. Towards a portable microchip system with integrated thermal control and polymer waveguides for real‐time PCR
AU2001235856B2 (en) Method for analysing the length of a nucleic acid molecule
JP2019532647A5 (enExample)
Batten et al. Real-time reverse transcriptase PCR for the detection of bluetongue virus
CN113699033A (zh) 一种基于熔解曲线的多重数字核酸分析装置和分析方法
WO2008080254A1 (en) Reaction buffer for microarray
Nilsson et al. Single‐Nucleotide Sequence Discrimination In Situ Using Padlock Probes
Yetisen et al. Nazente Atceken, Muhammad Munzer Alseed, Sajjad Rahmani Dabbagh

Legal Events

Date Code Title Description
FA92 Acknowledgement of application withdrawn (lack of supplementary materials submitted)

Effective date: 20210716