IL261980B2 - Surface-based detection of nucleic acids in convection flow fluidic devices - Google Patents

Surface-based detection of nucleic acids in convection flow fluidic devices

Info

Publication number
IL261980B2
IL261980B2 IL261980A IL26198018A IL261980B2 IL 261980 B2 IL261980 B2 IL 261980B2 IL 261980 A IL261980 A IL 261980A IL 26198018 A IL26198018 A IL 26198018A IL 261980 B2 IL261980 B2 IL 261980B2
Authority
IL
Israel
Prior art keywords
oligonucleotide
sequence
reaction chamber
dna
dna sequence
Prior art date
Application number
IL261980A
Other languages
English (en)
Hebrew (he)
Other versions
IL261980A (en
IL261980B1 (en
Inventor
A Khodakov Dmitriy
Zhang David
Original Assignee
Univ Rice William M
A Khodakov Dmitriy
Zhang David
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rice William M, A Khodakov Dmitriy, Zhang David filed Critical Univ Rice William M
Publication of IL261980A publication Critical patent/IL261980A/en
Publication of IL261980B1 publication Critical patent/IL261980B1/en
Publication of IL261980B2 publication Critical patent/IL261980B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
IL261980A 2016-03-29 2018-09-26 Surface-based detection of nucleic acids in convection flow fluidic devices IL261980B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662314909P 2016-03-29 2016-03-29
PCT/US2017/024530 WO2017172760A1 (en) 2016-03-29 2017-03-28 Surface-based detection of nucleic acid in a convection flow fluidic device

Publications (3)

Publication Number Publication Date
IL261980A IL261980A (en) 2018-10-31
IL261980B1 IL261980B1 (en) 2023-04-01
IL261980B2 true IL261980B2 (en) 2023-08-01

Family

ID=59965134

Family Applications (1)

Application Number Title Priority Date Filing Date
IL261980A IL261980B2 (en) 2016-03-29 2018-09-26 Surface-based detection of nucleic acids in convection flow fluidic devices

Country Status (15)

Country Link
US (2) US20200324292A1 (enExample)
EP (2) EP4063014A1 (enExample)
JP (3) JP6998319B2 (enExample)
KR (2) KR102472071B1 (enExample)
CN (1) CN109477136A (enExample)
AU (2) AU2017241766A1 (enExample)
BR (1) BR112018070197A2 (enExample)
DK (1) DK3436198T3 (enExample)
ES (1) ES2920954T3 (enExample)
IL (1) IL261980B2 (enExample)
PH (1) PH12018502100A1 (enExample)
PT (1) PT3436198T (enExample)
RU (1) RU2018137800A (enExample)
SG (1) SG11201808493QA (enExample)
WO (1) WO2017172760A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12599908B2 (en) * 2018-09-14 2026-04-14 William Marsh Rice University Apparatus and methods for multiplexed amplification and detection of DNA using convectional heating and label-free microarray
CN113257351A (zh) * 2020-02-12 2021-08-13 赛纳生物科技(北京)有限公司 一种用于多碱基基因测序的基因文库及其构建方法
CN111879922A (zh) * 2020-07-13 2020-11-03 东南大学 适用于核酸及免疫同步检测集成纸基芯片结构及制造方法
DE102020212253A1 (de) * 2020-09-29 2022-03-31 SpinDiag GmbH Probenträger und Rotationsvorrichtung
EP4640315A1 (en) * 2024-04-26 2025-10-29 Appolon Bioteck Devices and methods for rapid nucleic acid amplification

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US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US6642000B1 (en) 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides
JP2003522963A (ja) * 2000-02-18 2003-07-29 アクララ バイオサイエンシーズ， インコーポレイテッド 複数部位反応デバイスおよび方法
US6586233B2 (en) 2001-03-09 2003-07-01 The Regents Of The University Of California Convectively driven PCR thermal-cycling
RU2216547C2 (ru) 2001-10-16 2003-11-20 Институт молекулярной биологии им. В.А. Энгельгардта РАН Способ полимеризационной иммобилизации биологических макромолекул и композиция для его осуществления
WO2004033728A2 (en) * 2002-10-11 2004-04-22 Erasmus Universiteit Rotterdam Nucleic acid amplification primers for pcr-based clonality studies
EP1616947B1 (en) * 2003-03-28 2012-05-09 Japan as represented by Director General of National Rehabilitation Center for Persons with Disabilities METHOD OF SYNTHESIZING cDNA
KR100647289B1 (ko) * 2004-09-15 2006-11-23 삼성전자주식회사 마랑고니 대류를 이용한 ｐｃｒ 장치 및 이를 이용한ｐｃｒ 방법
US20060223122A1 (en) * 2005-03-08 2006-10-05 Agnes Fogo Classifying and predicting glomerulosclerosis using a proteomics approach
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CN103805507B (zh) 2008-01-24 2016-04-13 基亚生物科技股份有限公司 热对流聚合酶连锁反应之方法及装置
KR20100025328A (ko) * 2008-08-27 2010-03-09 삼성전자주식회사 이중가닥 영역과 말단 단일가닥 영역을 포함하는 이중가닥 핵산 프로브가 고정된 마이크로어레이를 제조하는 방법
CN102725424B (zh) * 2010-01-25 2014-07-09 Rd生物科技公司 靶核酸的自折叠扩增
WO2011097424A1 (en) * 2010-02-05 2011-08-11 Siemens Healthcare Diagnostics Inc. Methods for increasing multiplex level by externalization of passive reference in polymerase chain reactions
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Also Published As

Publication number Publication date
US20200324292A1 (en) 2020-10-15
US20240278249A1 (en) 2024-08-22
KR20190021198A (ko) 2019-03-05
JP2022037151A (ja) 2022-03-08
RU2018137800A3 (enExample) 2020-06-18
EP3436198A4 (en) 2019-10-30
PT3436198T (pt) 2022-07-13
PH12018502100A1 (en) 2019-07-29
JP6998319B2 (ja) 2022-01-18
IL261980A (en) 2018-10-31
CA3019422A1 (en) 2017-10-05
RU2018137800A (ru) 2020-04-29
BR112018070197A2 (pt) 2019-01-29
EP3436198B1 (en) 2022-05-04
JP2019509751A (ja) 2019-04-11
EP4063014A1 (en) 2022-09-28
IL261980B1 (en) 2023-04-01
JP2023184748A (ja) 2023-12-28
AU2023203972A1 (en) 2023-07-13
ES2920954T3 (es) 2022-08-12
SG11201808493QA (en) 2018-10-30
KR102472071B1 (ko) 2022-11-29
AU2017241766A1 (en) 2018-10-25
WO2017172760A1 (en) 2017-10-05
KR20230004792A (ko) 2023-01-06
KR102789837B1 (ko) 2025-04-01
CN109477136A (zh) 2019-03-15
DK3436198T3 (en) 2022-06-27
EP3436198A1 (en) 2019-02-06

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