KR102472071B1 - 대류 유체 디바이스에서의 핵산의 표면-기반 검출 - Google Patents

대류 유체 디바이스에서의 핵산의 표면-기반 검출 Download PDF

Info

Publication number
KR102472071B1
KR102472071B1 KR1020187030052A KR20187030052A KR102472071B1 KR 102472071 B1 KR102472071 B1 KR 102472071B1 KR 1020187030052 A KR1020187030052 A KR 1020187030052A KR 20187030052 A KR20187030052 A KR 20187030052A KR 102472071 B1 KR102472071 B1 KR 102472071B1
Authority
KR
South Korea
Prior art keywords
delete delete
oligonucleotide
sequence
reaction chamber
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020187030052A
Other languages
English (en)
Korean (ko)
Other versions
KR20190021198A (ko
Inventor
드미트리 에이 코다코브
데이비드 장
Original Assignee
윌리엄 마쉬 라이스 유니버시티
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 윌리엄 마쉬 라이스 유니버시티 filed Critical 윌리엄 마쉬 라이스 유니버시티
Priority to KR1020227041286A priority Critical patent/KR102789837B1/ko
Publication of KR20190021198A publication Critical patent/KR20190021198A/ko
Application granted granted Critical
Publication of KR102472071B1 publication Critical patent/KR102472071B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020187030052A 2016-03-29 2017-03-28 대류 유체 디바이스에서의 핵산의 표면-기반 검출 Active KR102472071B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020227041286A KR102789837B1 (ko) 2016-03-29 2017-03-28 대류 유체 디바이스에서의 핵산의 표면-기반 검출

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662314909P 2016-03-29 2016-03-29
US62/314,909 2016-03-29
PCT/US2017/024530 WO2017172760A1 (en) 2016-03-29 2017-03-28 Surface-based detection of nucleic acid in a convection flow fluidic device

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020227041286A Division KR102789837B1 (ko) 2016-03-29 2017-03-28 대류 유체 디바이스에서의 핵산의 표면-기반 검출

Publications (2)

Publication Number Publication Date
KR20190021198A KR20190021198A (ko) 2019-03-05
KR102472071B1 true KR102472071B1 (ko) 2022-11-29

Family

ID=59965134

Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020187030052A Active KR102472071B1 (ko) 2016-03-29 2017-03-28 대류 유체 디바이스에서의 핵산의 표면-기반 검출
KR1020227041286A Active KR102789837B1 (ko) 2016-03-29 2017-03-28 대류 유체 디바이스에서의 핵산의 표면-기반 검출

Family Applications After (1)

Application Number Title Priority Date Filing Date
KR1020227041286A Active KR102789837B1 (ko) 2016-03-29 2017-03-28 대류 유체 디바이스에서의 핵산의 표면-기반 검출

Country Status (16)

Country Link
US (2) US20200324292A1 (enExample)
EP (2) EP4063014A1 (enExample)
JP (3) JP6998319B2 (enExample)
KR (2) KR102472071B1 (enExample)
CN (1) CN109477136A (enExample)
AU (2) AU2017241766A1 (enExample)
BR (1) BR112018070197A2 (enExample)
CA (1) CA3019422A1 (enExample)
DK (1) DK3436198T3 (enExample)
ES (1) ES2920954T3 (enExample)
IL (1) IL261980B2 (enExample)
PH (1) PH12018502100A1 (enExample)
PT (1) PT3436198T (enExample)
RU (1) RU2018137800A (enExample)
SG (1) SG11201808493QA (enExample)
WO (1) WO2017172760A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112996601B (zh) * 2018-09-14 2024-10-29 威廉马歇莱思大学 使用对流加热和无标记微阵列对dna进行多重扩增和检测的装置和方法
CN113257351A (zh) * 2020-02-12 2021-08-13 赛纳生物科技(北京)有限公司 一种用于多碱基基因测序的基因文库及其构建方法
CN111879922A (zh) * 2020-07-13 2020-11-03 东南大学 适用于核酸及免疫同步检测集成纸基芯片结构及制造方法
DE102020212253A1 (de) * 2020-09-29 2022-03-31 SpinDiag GmbH Probenträger und Rotationsvorrichtung
EP4640315A1 (en) * 2024-04-26 2025-10-29 Appolon Bioteck Devices and methods for rapid nucleic acid amplification

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100056397A1 (en) * 2008-08-27 2010-03-04 Joo-Won Rhee Method of Producing Microarray Having Immobilized Double-Stranded Nucleic Acid Probe Including Double-Stranded Region and Single-Stranded Region

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027880A (en) 1995-08-02 2000-02-22 Affymetrix, Inc. Arrays of nucleic acid probes and methods of using the same for detecting cystic fibrosis
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US6642000B1 (en) 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides
AU2001238325A1 (en) * 2000-02-18 2001-08-27 Aclara Biosciences, Inc. Multiple-site reaction device and method
US6586233B2 (en) 2001-03-09 2003-07-01 The Regents Of The University Of California Convectively driven PCR thermal-cycling
US7846656B2 (en) 2001-10-16 2010-12-07 Institut Molekulyarnoi Biologii Im.V.A. Engelgardta Rossiiskoi Akademii Nauk Composition for polymerizing immobilization of biological molecules and method for producing said composition
JP4708029B2 (ja) * 2002-10-11 2011-06-22 エラスマス・ユニバーシティー・ロッテルダム Ｐｃｒベースのクローン性研究のための核酸増幅プライマー
US8067205B2 (en) * 2003-03-28 2011-11-29 Japan As Represented By Director General Of National Rehabilitation Center For Persons With Disabilities Method of synthesizing cDNA
KR100647289B1 (ko) * 2004-09-15 2006-11-23 삼성전자주식회사 마랑고니 대류를 이용한 ｐｃｒ 장치 및 이를 이용한ｐｃｒ 방법
US20060223122A1 (en) * 2005-03-08 2006-10-05 Agnes Fogo Classifying and predicting glomerulosclerosis using a proteomics approach
GB2424946A (en) * 2005-04-05 2006-10-11 Stratec Biomedical Systems Ag A detection system for substance binding using up-converting fluorescent probes
EP1943348B1 (en) * 2005-10-03 2013-01-02 Life Technologies Corporation Compositions, methods, and kits for amplifying nucleic acids
US8114636B2 (en) * 2006-02-10 2012-02-14 Life Technologies Corporation Labeling and detection of nucleic acids
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
US8735103B2 (en) 2006-12-05 2014-05-27 Electronics And Telecommunications Research Institute Natural convection-driven PCR apparatus and method using disposable polymer chip
WO2008097929A2 (en) 2007-02-05 2008-08-14 California Institute Of Technology Engineered toehold reactions and networks
US8043810B2 (en) 2007-05-02 2011-10-25 Eagle Eye Research, Inc. Analyte detection using autocatalytic chain reactions
EP2610007B1 (en) * 2007-07-23 2018-12-05 CLONDIAG GmbH Assays
AU2009206216B2 (en) 2008-01-24 2014-11-13 Medigen Biotechnology Corp. Methods and apparatuses for convective polymerase chain reaction (PCR)
WO2011091393A1 (en) * 2010-01-25 2011-07-28 Rd Biosciences, Inc. Self-folding amplification of target nucleic acid
WO2011097424A1 (en) * 2010-02-05 2011-08-11 Siemens Healthcare Diagnostics Inc. Methods for increasing multiplex level by externalization of passive reference in polymerase chain reactions
ES2623859T3 (es) * 2010-03-04 2017-07-12 Miacom Diagnostics Gmbh FISH múltiple mejorada
EP2577534A4 (en) * 2010-05-27 2017-04-26 Emerald Therapeutics, Inc. System and method for propagating information using modified nucleic acids
EP2633071B1 (en) * 2010-10-27 2016-10-12 President and Fellows of Harvard College Compositions of toehold primer duplexes and methods of use
WO2012162161A1 (en) * 2011-05-20 2012-11-29 Phthisis Diagnostics Microsporidia detection system and method
US20140302486A1 (en) * 2011-09-02 2014-10-09 President And Fellows Of Harvard College Systems and methods for detecting biomarkers of interest
JP6427753B2 (ja) * 2013-09-11 2018-11-28 国立大学法人大阪大学 熱対流生成用チップ、熱対流生成装置、及び熱対流生成方法
EP3084051B1 (en) * 2013-12-16 2020-01-01 William Marsh Rice University Fine-tuned ultraspecific nucleic acid hybridization probes
CN108463560B (zh) * 2016-01-15 2022-09-20 昆塔波尔公司 具有降低的背景的基于光学的纳米孔分析

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100056397A1 (en) * 2008-08-27 2010-03-04 Joo-Won Rhee Method of Producing Microarray Having Immobilized Double-Stranded Nucleic Acid Probe Including Double-Stranded Region and Single-Stranded Region

Also Published As

Publication number Publication date
SG11201808493QA (en) 2018-10-30
WO2017172760A1 (en) 2017-10-05
US20240278249A1 (en) 2024-08-22
PT3436198T (pt) 2022-07-13
BR112018070197A2 (pt) 2019-01-29
KR20230004792A (ko) 2023-01-06
KR20190021198A (ko) 2019-03-05
IL261980B1 (en) 2023-04-01
JP2023184748A (ja) 2023-12-28
US20200324292A1 (en) 2020-10-15
JP2019509751A (ja) 2019-04-11
AU2023203972A1 (en) 2023-07-13
IL261980B2 (en) 2023-08-01
JP2022037151A (ja) 2022-03-08
AU2017241766A1 (en) 2018-10-25
EP3436198B1 (en) 2022-05-04
RU2018137800A3 (enExample) 2020-06-18
CA3019422A1 (en) 2017-10-05
KR102789837B1 (ko) 2025-04-01
ES2920954T3 (es) 2022-08-12
EP3436198A4 (en) 2019-10-30
EP4063014A1 (en) 2022-09-28
RU2018137800A (ru) 2020-04-29
CN109477136A (zh) 2019-03-15
JP6998319B2 (ja) 2022-01-18
EP3436198A1 (en) 2019-02-06
IL261980A (en) 2018-10-31
DK3436198T3 (en) 2022-06-27
PH12018502100A1 (en) 2019-07-29

Similar Documents

Publication Publication Date Title
US20240278249A1 (en) Surface-based detection of nucleic acid in a convection flow fluidic device
EP3458597B1 (en) Quantitative real time pcr amplification using an electrowetting-based device
US11293053B2 (en) Bifunctional oligonucleotide probe for universal real time multianalyte detection
JP2012509078A (ja) 二本鎖核酸特異色素を用いる固体表面でのリアルタイムマルチプレックスｐｃｒ検出
US8017327B2 (en) Single nucleotide polymorphism genotyping detection via the real-time invader assay microarray platform
SK12212000A3 (sk) Metóda na detekciu cieľovej nukleovej kyseliny pomocou PCR
CN102428189A (zh) 反应筒内多重核酸序列的检测
CN115515626A (zh) 用于检测冠状病毒的组合物和方法
KR102323375B1 (ko) 다중 프로브
JP4297966B2 (ja) 標識ヌクレオチドを利用することによる核酸の新規測定方法
JP2025060794A (ja) 液相及び固相ｄｎａ増幅の組合せ
JP4276897B2 (ja) 標識ヌクレオチドを利用することによる核酸の新規測定方法
KR20120045909A (ko) 복수 종의 표적 핵산을 실시간으로 검출하는 장치 및 방법
JP2007014266A (ja) 核酸の解析法

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

R17-X000 Change to representative recorded

St.27 status event code: A-3-3-R10-R17-oth-X000

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

GRNT Written decision to grant
PA0104 Divisional application for international application

St.27 status event code: A-0-1-A10-A18-div-PA0104

St.27 status event code: A-0-1-A10-A16-div-PA0104

PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-OTH-PR1001 (AS PROVIDED BY THE NATIONAL OFFICE)

Year of fee payment: 4