CN109477136A - 对流流动的流体装置中核酸基于表面的检测 - Google Patents

对流流动的流体装置中核酸基于表面的检测 Download PDF

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Publication number
CN109477136A
CN109477136A CN201780026551.7A CN201780026551A CN109477136A CN 109477136 A CN109477136 A CN 109477136A CN 201780026551 A CN201780026551 A CN 201780026551A CN 109477136 A CN109477136 A CN 109477136A
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China
Prior art keywords
reaction chamber
dna
sequence
oligonucleotide
nucleotides
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CN201780026551.7A
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English (en)
Chinese (zh)
Inventor
德米特里·A·霍达科夫
大卫·张
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William Marsh Rice University
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William Marsh Rice University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201780026551.7A 2016-03-29 2017-03-28 对流流动的流体装置中核酸基于表面的检测 Pending CN109477136A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662314909P 2016-03-29 2016-03-29
US62/314,909 2016-03-29
PCT/US2017/024530 WO2017172760A1 (en) 2016-03-29 2017-03-28 Surface-based detection of nucleic acid in a convection flow fluidic device

Publications (1)

Publication Number Publication Date
CN109477136A true CN109477136A (zh) 2019-03-15

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CN201780026551.7A Pending CN109477136A (zh) 2016-03-29 2017-03-28 对流流动的流体装置中核酸基于表面的检测

Country Status (16)

Country Link
US (2) US20200324292A1 (enExample)
EP (2) EP4063014A1 (enExample)
JP (3) JP6998319B2 (enExample)
KR (2) KR102472071B1 (enExample)
CN (1) CN109477136A (enExample)
AU (2) AU2017241766A1 (enExample)
BR (1) BR112018070197A2 (enExample)
CA (1) CA3019422A1 (enExample)
DK (1) DK3436198T3 (enExample)
ES (1) ES2920954T3 (enExample)
IL (1) IL261980B2 (enExample)
PH (1) PH12018502100A1 (enExample)
PT (1) PT3436198T (enExample)
RU (1) RU2018137800A (enExample)
SG (1) SG11201808493QA (enExample)
WO (1) WO2017172760A1 (enExample)

Cited By (2)

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CN111879922A (zh) * 2020-07-13 2020-11-03 东南大学 适用于核酸及免疫同步检测集成纸基芯片结构及制造方法
CN113257351A (zh) * 2020-02-12 2021-08-13 赛纳生物科技(北京)有限公司 一种用于多碱基基因测序的基因文库及其构建方法

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* Cited by examiner, † Cited by third party
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CN112996601B (zh) * 2018-09-14 2024-10-29 威廉马歇莱思大学 使用对流加热和无标记微阵列对dna进行多重扩增和检测的装置和方法
DE102020212253A1 (de) * 2020-09-29 2022-03-31 SpinDiag GmbH Probenträger und Rotationsvorrichtung
EP4640315A1 (en) * 2024-04-26 2025-10-29 Appolon Bioteck Devices and methods for rapid nucleic acid amplification

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Publication number Priority date Publication date Assignee Title
CN113257351A (zh) * 2020-02-12 2021-08-13 赛纳生物科技(北京)有限公司 一种用于多碱基基因测序的基因文库及其构建方法
CN111879922A (zh) * 2020-07-13 2020-11-03 东南大学 适用于核酸及免疫同步检测集成纸基芯片结构及制造方法

Also Published As

Publication number Publication date
SG11201808493QA (en) 2018-10-30
WO2017172760A1 (en) 2017-10-05
US20240278249A1 (en) 2024-08-22
PT3436198T (pt) 2022-07-13
BR112018070197A2 (pt) 2019-01-29
KR20230004792A (ko) 2023-01-06
KR20190021198A (ko) 2019-03-05
IL261980B1 (en) 2023-04-01
JP2023184748A (ja) 2023-12-28
US20200324292A1 (en) 2020-10-15
JP2019509751A (ja) 2019-04-11
AU2023203972A1 (en) 2023-07-13
IL261980B2 (en) 2023-08-01
JP2022037151A (ja) 2022-03-08
AU2017241766A1 (en) 2018-10-25
EP3436198B1 (en) 2022-05-04
RU2018137800A3 (enExample) 2020-06-18
CA3019422A1 (en) 2017-10-05
KR102789837B1 (ko) 2025-04-01
KR102472071B1 (ko) 2022-11-29
ES2920954T3 (es) 2022-08-12
EP3436198A4 (en) 2019-10-30
EP4063014A1 (en) 2022-09-28
RU2018137800A (ru) 2020-04-29
JP6998319B2 (ja) 2022-01-18
EP3436198A1 (en) 2019-02-06
IL261980A (en) 2018-10-31
DK3436198T3 (en) 2022-06-27
PH12018502100A1 (en) 2019-07-29

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