JP6320621B2 - プシコースエピマー化酵素及びこれを利用したプシコースの製造方法 - Google Patents

プシコースエピマー化酵素及びこれを利用したプシコースの製造方法 Download PDF

Info

Publication number
JP6320621B2
JP6320621B2 JP2017503758A JP2017503758A JP6320621B2 JP 6320621 B2 JP6320621 B2 JP 6320621B2 JP 2017503758 A JP2017503758 A JP 2017503758A JP 2017503758 A JP2017503758 A JP 2017503758A JP 6320621 B2 JP6320621 B2 JP 6320621B2
Authority
JP
Japan
Prior art keywords
psicose
enzyme
fructose
producing
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017503758A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017510302A (ja
JP2017510302A5 (cg-RX-API-DMAC7.html
Inventor
キム・テギュン
キム・ミンス
キム・テヨン
ソン・ウンボム
オ・ドクン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Daesang Corp
Original Assignee
Daesang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daesang Corp filed Critical Daesang Corp
Publication of JP2017510302A publication Critical patent/JP2017510302A/ja
Publication of JP2017510302A5 publication Critical patent/JP2017510302A5/ja
Application granted granted Critical
Publication of JP6320621B2 publication Critical patent/JP6320621B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90Isomerases (5.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/02Monosaccharides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/24Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y501/00Racemaces and epimerases (5.1)
    • C12Y501/03Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
JP2017503758A 2014-05-28 2015-05-22 プシコースエピマー化酵素及びこれを利用したプシコースの製造方法 Active JP6320621B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20140064651A KR101473918B1 (ko) 2014-05-28 2014-05-28 사이코스 에피머화 효소, 이의 제조방법 및 이를 이용한 사이코스의 제조방법
KR10-2014-0064651 2014-05-28
PCT/KR2015/005208 WO2015182937A1 (ko) 2014-05-28 2015-05-22 사이코스 에피머화 효소 및 이를 이용한 사이코스의 제조방법

Publications (3)

Publication Number Publication Date
JP2017510302A JP2017510302A (ja) 2017-04-13
JP2017510302A5 JP2017510302A5 (cg-RX-API-DMAC7.html) 2017-11-16
JP6320621B2 true JP6320621B2 (ja) 2018-05-09

Family

ID=52679228

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017503758A Active JP6320621B2 (ja) 2014-05-28 2015-05-22 プシコースエピマー化酵素及びこれを利用したプシコースの製造方法

Country Status (6)

Country Link
US (1) US9988618B2 (cg-RX-API-DMAC7.html)
EP (1) EP3135762B1 (cg-RX-API-DMAC7.html)
JP (1) JP6320621B2 (cg-RX-API-DMAC7.html)
KR (1) KR101473918B1 (cg-RX-API-DMAC7.html)
CN (1) CN106164265B (cg-RX-API-DMAC7.html)
WO (1) WO2015182937A1 (cg-RX-API-DMAC7.html)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2796749T3 (es) 2014-10-30 2020-11-30 Samyang Corp Sistema de expresión de psicosa epimerasa y producción de psicosa que utiliza la misma
KR101656063B1 (ko) * 2015-05-22 2016-09-08 주식회사 삼양사 사이코스 에퍼머화 효소의 발현 시스템 및 이를 이용한 사이코스의 생산
KR102087396B1 (ko) 2015-11-16 2020-03-10 주식회사 삼양사 과당-함유 기질로부터 사이코스를 생산하는 방법
KR101944103B1 (ko) * 2015-12-07 2019-01-30 주식회사 삼양사 마이크로박테리움 속 균주 및 이를 이용한 사이코스 생산방법
CN106434494B (zh) * 2016-12-02 2017-11-07 山东百龙创园生物科技股份有限公司 一株枯草芽孢杆菌及其培养方法与应用
CN106520746B (zh) 2016-12-02 2017-12-26 山东百龙创园生物科技股份有限公司 一种高纯度d‑阿洛酮糖的制备方法
KR102065155B1 (ko) 2016-12-08 2020-02-11 주식회사 삼양사 사이코스의 제조방법
EP3555282A4 (en) * 2016-12-14 2020-07-22 Bonumose LLC ENZYMATIC PRODUCTION OF D-ALLULOSE
CN108531527A (zh) * 2017-03-03 2018-09-14 上海立足生物科技有限公司 一种d-阿洛酮糖-3-差向异构酶的应用
KR101944104B1 (ko) * 2017-06-02 2019-01-30 주식회사 삼양사 마이크로박테리움 속 균주 및 이를 이용한 사이코스 생산방법
JP6973898B2 (ja) * 2017-07-31 2021-12-01 シージェイ チェイルジェダン コーポレーションCj Cheiljedang Corporation 新規のプシコース−6−リン酸脱リン酸酵素、前記酵素を含むプシコース生産用組成物、前記酵素を利用してプシコースを製造する方法
WO2019043088A1 (en) * 2017-08-31 2019-03-07 Novozymes A/S POLYPEPTIDES HAVING D-PSICOSE 3-EPIMERASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME
CN108588149B (zh) * 2017-10-29 2022-06-10 中国科学院天津工业生物技术研究所 一种阿果糖浆及其制备方法
KR101965509B1 (ko) * 2017-11-15 2019-04-03 씨제이제일제당 (주) 신규한 d-사이코스 3-에피머화 효소 및 이를 이용한 d-사이코스의 제조 방법
US11859228B2 (en) 2018-08-08 2024-01-02 Archer Daniels Midland Company Epimerase enzymes and their use
KR102068752B1 (ko) 2019-01-24 2020-01-21 주식회사 삼양사 마이크로박테리움 속 균주 및 이를 이용한 사이코스 생산방법
KR102054961B1 (ko) * 2019-01-24 2019-12-12 주식회사 삼양사 마이크로박테리움 속 균주 및 이를 이용한 사이코스 생산방법
KR102080886B1 (ko) * 2019-05-21 2020-02-24 씨제이제일제당 주식회사 부반응성이 낮은 리불로스-인산 3-에피머화 효소의 모티프 및 이를 포함하는 효소
CN111019928B (zh) * 2019-12-11 2022-08-16 吉林中粮生化有限公司 D-阿洛酮糖3-差向异构酶的编码基因、载体、重组细胞以及它们的应用
KR102254411B1 (ko) * 2019-12-19 2021-05-24 대상 주식회사 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법
KR102448351B1 (ko) * 2020-04-27 2022-09-28 대상 주식회사 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법
CN112831489B (zh) * 2020-09-18 2022-06-10 中国科学院天津工业生物技术研究所 一种阿洛酮糖3-差向异构酶固定化酶、其固定化方法与应用
CN113667707B (zh) * 2021-10-21 2022-04-15 中粮营养健康研究院有限公司 由葡萄糖生产d-阿洛酮糖的方法
KR102682846B1 (ko) 2021-11-19 2024-07-08 대상 주식회사 열 안정성이 우수한 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법
US20250075237A1 (en) * 2021-12-29 2025-03-06 Daesang Corporation Novel promoter variant for constitutive expression and use thereof
CN114262703A (zh) * 2021-12-31 2022-04-01 保龄宝生物股份有限公司 一种利用膜富集d-阿洛酮糖3-差向异构酶的方法及应用
CN114601745B (zh) * 2022-03-25 2023-06-27 上海龙殷生物科技有限公司 一种护肤品原料、化妆品、制备方法及应用
CN114561417B (zh) * 2022-04-27 2022-08-09 中国科学院天津工业生物技术研究所 用于制备阿洛酮糖的谷氨酸棒杆菌工程菌株及其应用
KR20250095788A (ko) 2023-12-19 2025-06-27 대상 주식회사 항시발현용 신규 프로모터, 이를 포함하는 목적 단백질 발현 시스템 및 이를 이용하여 알룰로스를 제조하는 방법
CN117965519A (zh) * 2024-04-02 2024-05-03 东晓生物科技股份有限公司 一种d-阿洛酮糖-3-差向异构酶固定化酶制剂的固定方法
CN119193635B (zh) * 2024-12-02 2025-03-25 中国水产科学研究院黄海水产研究所 南极来源d-阿洛酮糖3-差向异构酶基因的突变体及应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006129554A1 (en) 2005-05-30 2006-12-07 Fujifilm Corporation Album creating apparatus, album creating method and program
KR100744479B1 (ko) * 2005-06-01 2007-08-01 씨제이 주식회사 사이코스 에피머화 효소에 의한 사이코스의 생산 방법
JP5098086B2 (ja) 2005-11-15 2012-12-12 株式会社林原 ケトース3−エピメラーゼとその製造方法並びに用途
KR100832339B1 (ko) 2006-12-11 2008-05-26 솔젠트 (주) 과당을 사이코스로 전환하는 신규한 시노리조비움 속균주와 이를 이용한 사이코스 생산법
KR20110035805A (ko) 2009-09-30 2011-04-06 씨제이제일제당 (주) 사이코스-에피머화 효소의 고정화 및 이를 이용한 사이코스의 제조방법
KR101106253B1 (ko) 2009-10-16 2012-01-18 경상대학교산학협력단 사이코스 3-에피머라제 효소를 코딩하는 폴리뉴클레오티드를 포함하는 대장균 및 그를 이용하여 사이코스를 생산하는 방법
CN102373230A (zh) * 2010-08-27 2012-03-14 天津工业生物技术研究所 某种梭菌d-塔格糖3-差向异构酶的核苷酸序列及其应用
KR101203856B1 (ko) 2011-08-24 2012-11-21 씨제이제일제당 (주) 열 안정성이 향상된 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 연속적 생산
WO2014025235A1 (ko) * 2012-08-10 2014-02-13 주식회사 삼양제넥스 사이코스 에피머화 효소 및 이를 이용한 사이코스로 전환용 조성물
CN104769125B (zh) * 2012-08-10 2018-10-23 株式会社三养社 阿洛酮糖差向异构酶及使用其的用于转变为阿洛酮糖的组合物
GB2508586B (en) * 2012-09-27 2020-09-02 Tate & Lyle Ingredients Americas Llc A protein
KR101318422B1 (ko) 2013-04-09 2013-10-15 주식회사 삼양제넥스 D-사이코스 에피머화 효소, 및 이를 이용하는 사이코스 생산방법

Also Published As

Publication number Publication date
EP3135762A4 (en) 2017-09-20
WO2015182937A1 (ko) 2015-12-03
JP2017510302A (ja) 2017-04-13
US9988618B2 (en) 2018-06-05
KR101473918B1 (ko) 2014-12-17
EP3135762A1 (en) 2017-03-01
CN106164265B (zh) 2019-10-25
US20170101637A1 (en) 2017-04-13
CN106164265A (zh) 2016-11-23
EP3135762B1 (en) 2019-07-10

Similar Documents

Publication Publication Date Title
JP6320621B2 (ja) プシコースエピマー化酵素及びこれを利用したプシコースの製造方法
JP2017510302A5 (cg-RX-API-DMAC7.html)
US9938515B2 (en) Psicose epimerase mutant and method for preparing psicose by using same
JP6282746B2 (ja) プシコースエピメラーゼをコードするポリヌクレオチド、およびこれを用いるプシコース生産方法。
US11634740B2 (en) Allulose epimerase variant, method for preparing the same, and method for preparing allulose using the same
AU2007326238B2 (en) Arabinose isomerase expressed from corynebacterium genus and tagatose manufacturing method by using it
RU2727903C1 (ru) Новая d-псикозо-3-эпимераза и способ получения d-псикозы с ее использованием
US12098404B2 (en) Allulose epimerase variant, method of producing the same, and method of producing allulose using the same
KR101610911B1 (ko) L-리불로스 5-인산 4-에피머화 효소를 이용한 과당에서 타가토스 생산
JP7535192B2 (ja) 熱安定性に優れたアルロースエピマー化酵素変異体、その製造方法およびこれを用いたアルロースの製造方法
TWI707952B (zh) 新穎d-阿洛酮糖3-表異構酶及使用該d-阿洛酮糖3-表異構酶製造d-阿洛酮糖之方法
EP3865574B1 (en) Allulose epimerase variant, method for producing same, and method for producing allulose using same
KR101841267B1 (ko) 라이보스-5-인산 이성화효소, 이의 제조방법 및 이를 이용한 알로스의 제조방법

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20161004

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170718

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20171005

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20180109

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180216

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20180306

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20180403

R150 Certificate of patent or registration of utility model

Ref document number: 6320621

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250