JP2017510302A5 - - Google Patents
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- JP2017510302A5 JP2017510302A5 JP2017503758A JP2017503758A JP2017510302A5 JP 2017510302 A5 JP2017510302 A5 JP 2017510302A5 JP 2017503758 A JP2017503758 A JP 2017503758A JP 2017503758 A JP2017503758 A JP 2017503758A JP 2017510302 A5 JP2017510302 A5 JP 2017510302A5
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- Prior art keywords
- psicose
- enzyme
- fructose
- recombinant
- activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20140064651A KR101473918B1 (ko) | 2014-05-28 | 2014-05-28 | 사이코스 에피머화 효소, 이의 제조방법 및 이를 이용한 사이코스의 제조방법 |
| KR10-2014-0064651 | 2014-05-28 | ||
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Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2796749T3 (es) | 2014-10-30 | 2020-11-30 | Samyang Corp | Sistema de expresión de psicosa epimerasa y producción de psicosa que utiliza la misma |
| KR101656063B1 (ko) * | 2015-05-22 | 2016-09-08 | 주식회사 삼양사 | 사이코스 에퍼머화 효소의 발현 시스템 및 이를 이용한 사이코스의 생산 |
| KR102087396B1 (ko) | 2015-11-16 | 2020-03-10 | 주식회사 삼양사 | 과당-함유 기질로부터 사이코스를 생산하는 방법 |
| KR101944103B1 (ko) * | 2015-12-07 | 2019-01-30 | 주식회사 삼양사 | 마이크로박테리움 속 균주 및 이를 이용한 사이코스 생산방법 |
| CN106434494B (zh) * | 2016-12-02 | 2017-11-07 | 山东百龙创园生物科技股份有限公司 | 一株枯草芽孢杆菌及其培养方法与应用 |
| CN106520746B (zh) | 2016-12-02 | 2017-12-26 | 山东百龙创园生物科技股份有限公司 | 一种高纯度d‑阿洛酮糖的制备方法 |
| KR102065155B1 (ko) | 2016-12-08 | 2020-02-11 | 주식회사 삼양사 | 사이코스의 제조방법 |
| EP3555282A4 (en) * | 2016-12-14 | 2020-07-22 | Bonumose LLC | ENZYMATIC PRODUCTION OF D-ALLULOSE |
| CN108531527A (zh) * | 2017-03-03 | 2018-09-14 | 上海立足生物科技有限公司 | 一种d-阿洛酮糖-3-差向异构酶的应用 |
| KR101944104B1 (ko) * | 2017-06-02 | 2019-01-30 | 주식회사 삼양사 | 마이크로박테리움 속 균주 및 이를 이용한 사이코스 생산방법 |
| JP6973898B2 (ja) * | 2017-07-31 | 2021-12-01 | シージェイ チェイルジェダン コーポレーションCj Cheiljedang Corporation | 新規のプシコース−6−リン酸脱リン酸酵素、前記酵素を含むプシコース生産用組成物、前記酵素を利用してプシコースを製造する方法 |
| WO2019043088A1 (en) * | 2017-08-31 | 2019-03-07 | Novozymes A/S | POLYPEPTIDES HAVING D-PSICOSE 3-EPIMERASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME |
| CN108588149B (zh) * | 2017-10-29 | 2022-06-10 | 中国科学院天津工业生物技术研究所 | 一种阿果糖浆及其制备方法 |
| KR101965509B1 (ko) * | 2017-11-15 | 2019-04-03 | 씨제이제일제당 (주) | 신규한 d-사이코스 3-에피머화 효소 및 이를 이용한 d-사이코스의 제조 방법 |
| US11859228B2 (en) | 2018-08-08 | 2024-01-02 | Archer Daniels Midland Company | Epimerase enzymes and their use |
| KR102068752B1 (ko) | 2019-01-24 | 2020-01-21 | 주식회사 삼양사 | 마이크로박테리움 속 균주 및 이를 이용한 사이코스 생산방법 |
| KR102054961B1 (ko) * | 2019-01-24 | 2019-12-12 | 주식회사 삼양사 | 마이크로박테리움 속 균주 및 이를 이용한 사이코스 생산방법 |
| KR102080886B1 (ko) * | 2019-05-21 | 2020-02-24 | 씨제이제일제당 주식회사 | 부반응성이 낮은 리불로스-인산 3-에피머화 효소의 모티프 및 이를 포함하는 효소 |
| CN111019928B (zh) * | 2019-12-11 | 2022-08-16 | 吉林中粮生化有限公司 | D-阿洛酮糖3-差向异构酶的编码基因、载体、重组细胞以及它们的应用 |
| KR102254411B1 (ko) * | 2019-12-19 | 2021-05-24 | 대상 주식회사 | 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법 |
| KR102448351B1 (ko) * | 2020-04-27 | 2022-09-28 | 대상 주식회사 | 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법 |
| CN112831489B (zh) * | 2020-09-18 | 2022-06-10 | 中国科学院天津工业生物技术研究所 | 一种阿洛酮糖3-差向异构酶固定化酶、其固定化方法与应用 |
| CN113667707B (zh) * | 2021-10-21 | 2022-04-15 | 中粮营养健康研究院有限公司 | 由葡萄糖生产d-阿洛酮糖的方法 |
| KR102682846B1 (ko) | 2021-11-19 | 2024-07-08 | 대상 주식회사 | 열 안정성이 우수한 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법 |
| US20250075237A1 (en) * | 2021-12-29 | 2025-03-06 | Daesang Corporation | Novel promoter variant for constitutive expression and use thereof |
| CN114262703A (zh) * | 2021-12-31 | 2022-04-01 | 保龄宝生物股份有限公司 | 一种利用膜富集d-阿洛酮糖3-差向异构酶的方法及应用 |
| CN114601745B (zh) * | 2022-03-25 | 2023-06-27 | 上海龙殷生物科技有限公司 | 一种护肤品原料、化妆品、制备方法及应用 |
| CN114561417B (zh) * | 2022-04-27 | 2022-08-09 | 中国科学院天津工业生物技术研究所 | 用于制备阿洛酮糖的谷氨酸棒杆菌工程菌株及其应用 |
| KR20250095788A (ko) | 2023-12-19 | 2025-06-27 | 대상 주식회사 | 항시발현용 신규 프로모터, 이를 포함하는 목적 단백질 발현 시스템 및 이를 이용하여 알룰로스를 제조하는 방법 |
| CN117965519A (zh) * | 2024-04-02 | 2024-05-03 | 东晓生物科技股份有限公司 | 一种d-阿洛酮糖-3-差向异构酶固定化酶制剂的固定方法 |
| CN119193635B (zh) * | 2024-12-02 | 2025-03-25 | 中国水产科学研究院黄海水产研究所 | 南极来源d-阿洛酮糖3-差向异构酶基因的突变体及应用 |
Family Cites Families (12)
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| WO2006129554A1 (en) | 2005-05-30 | 2006-12-07 | Fujifilm Corporation | Album creating apparatus, album creating method and program |
| KR100744479B1 (ko) * | 2005-06-01 | 2007-08-01 | 씨제이 주식회사 | 사이코스 에피머화 효소에 의한 사이코스의 생산 방법 |
| JP5098086B2 (ja) | 2005-11-15 | 2012-12-12 | 株式会社林原 | ケトース3−エピメラーゼとその製造方法並びに用途 |
| KR100832339B1 (ko) | 2006-12-11 | 2008-05-26 | 솔젠트 (주) | 과당을 사이코스로 전환하는 신규한 시노리조비움 속균주와 이를 이용한 사이코스 생산법 |
| KR20110035805A (ko) | 2009-09-30 | 2011-04-06 | 씨제이제일제당 (주) | 사이코스-에피머화 효소의 고정화 및 이를 이용한 사이코스의 제조방법 |
| KR101106253B1 (ko) | 2009-10-16 | 2012-01-18 | 경상대학교산학협력단 | 사이코스 3-에피머라제 효소를 코딩하는 폴리뉴클레오티드를 포함하는 대장균 및 그를 이용하여 사이코스를 생산하는 방법 |
| CN102373230A (zh) * | 2010-08-27 | 2012-03-14 | 天津工业生物技术研究所 | 某种梭菌d-塔格糖3-差向异构酶的核苷酸序列及其应用 |
| KR101203856B1 (ko) | 2011-08-24 | 2012-11-21 | 씨제이제일제당 (주) | 열 안정성이 향상된 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 연속적 생산 |
| WO2014025235A1 (ko) * | 2012-08-10 | 2014-02-13 | 주식회사 삼양제넥스 | 사이코스 에피머화 효소 및 이를 이용한 사이코스로 전환용 조성물 |
| CN104769125B (zh) * | 2012-08-10 | 2018-10-23 | 株式会社三养社 | 阿洛酮糖差向异构酶及使用其的用于转变为阿洛酮糖的组合物 |
| GB2508586B (en) * | 2012-09-27 | 2020-09-02 | Tate & Lyle Ingredients Americas Llc | A protein |
| KR101318422B1 (ko) | 2013-04-09 | 2013-10-15 | 주식회사 삼양제넥스 | D-사이코스 에피머화 효소, 및 이를 이용하는 사이코스 생산방법 |
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2014
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- 2015-05-22 EP EP15800046.3A patent/EP3135762B1/en active Active
- 2015-05-22 JP JP2017503758A patent/JP6320621B2/ja active Active
- 2015-05-22 US US15/128,812 patent/US9988618B2/en active Active
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