JP2018509915A5 - - Google Patents

Info

Publication number

JP2018509915A5

JP2018509915A5 JP2017549390A JP2017549390A JP2018509915A5 JP 2018509915 A5 JP2018509915 A5 JP 2018509915A5 JP 2017549390 A JP2017549390 A JP 2017549390A JP 2017549390 A JP2017549390 A JP 2017549390A JP 2018509915 A5 JP2018509915 A5 JP 2018509915A5

Authority

JP

Japan

Prior art keywords

barcode

barcodes

combinatorial

linker

label

Prior art date

2016-03-29

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
• Discuss

• 238000000034 method Methods 0.000 claims description 89
• 239000000203 mixture Substances 0.000 claims description 66
• 108091034117 Oligonucleotide Proteins 0.000 claims description 30
• 238000005192 partition Methods 0.000 claims description 30
• 239000000047 product Substances 0.000 claims description 14
• 108020004414 DNA Proteins 0.000 claims description 11
• 238000012163 sequencing technique Methods 0.000 claims description 11
• 239000000758 substrate Substances 0.000 claims description 11
• 230000003321 amplification Effects 0.000 claims description 10
• 238000003199 nucleic acid amplification method Methods 0.000 claims description 10
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 6
• 108020004999 messenger RNA Proteins 0.000 claims description 5
• 108020004707 nucleic acids Proteins 0.000 claims description 5
• 102000039446 nucleic acids Human genes 0.000 claims description 5
• 150000007523 nucleic acids Chemical class 0.000 claims description 5
• 238000011176 pooling Methods 0.000 claims description 5
• 239000007787 solid Substances 0.000 claims description 5
• 108020004459 Small interfering RNA Proteins 0.000 claims description 4
• 239000003153 chemical reaction reagent Substances 0.000 claims description 4
• 230000000295 complement effect Effects 0.000 claims description 4
• 239000004055 small Interfering RNA Substances 0.000 claims description 4
• 108091093088 Amplicon Proteins 0.000 claims description 3
• 238000003491 array Methods 0.000 claims description 3
• 108700011259 MicroRNAs Proteins 0.000 claims description 2
• 108091036407 Polyadenylation Proteins 0.000 claims description 2
• 239000007857 degradation product Substances 0.000 claims description 2
• 230000002934 lysing effect Effects 0.000 claims description 2
• 239000002679 microRNA Substances 0.000 claims description 2
• 108091033319 polynucleotide Proteins 0.000 claims 7
• 102000040430 polynucleotide Human genes 0.000 claims 7
• 239000002157 polynucleotide Substances 0.000 claims 7
• 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 4
• 239000011324 bead Substances 0.000 claims 2
• 239000002773 nucleotide Substances 0.000 claims 2
• 125000003729 nucleotide group Chemical group 0.000 claims 2
• 239000002245 particle Substances 0.000 claims 2
• 238000006243 chemical reaction Methods 0.000 claims 1
• -1 closed droplets Substances 0.000 claims 1
• 239000007863 gel particle Substances 0.000 claims 1
• 239000012510 hollow fiber Substances 0.000 claims 1
• 238000009396 hybridization Methods 0.000 claims 1
• 239000007788 liquid Substances 0.000 claims 1
• 108090000623 proteins and genes Proteins 0.000 claims 1
• 238000000926 separation method Methods 0.000 claims 1
• 210000004027 cell Anatomy 0.000 description 26
• 239000000523 sample Substances 0.000 description 23
• 239000012634 fragment Substances 0.000 description 14
• 230000002759 chromosomal effect Effects 0.000 description 8
• 229920002477 rna polymer Polymers 0.000 description 5
• 238000000151 deposition Methods 0.000 description 4
• 239000002299 complementary DNA Substances 0.000 description 3
• 102000054766 genetic haplotypes Human genes 0.000 description 3
• 238000012512 characterization method Methods 0.000 description 2
• 210000000349 chromosome Anatomy 0.000 description 2
• 238000006073 displacement reaction Methods 0.000 description 2
• 238000013507 mapping Methods 0.000 description 2
• 238000010186 staining Methods 0.000 description 2
• 210000001519 tissue Anatomy 0.000 description 2
• 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 1
• 206010028980 Neoplasm Diseases 0.000 description 1
• 239000013614 RNA sample Substances 0.000 description 1
• 210000004958 brain cell Anatomy 0.000 description 1
• 201000011510 cancer Diseases 0.000 description 1
• 239000013553 cell monolayer Substances 0.000 description 1
• 210000003040 circulating cell Anatomy 0.000 description 1
• SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
• SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
• 210000002919 epithelial cell Anatomy 0.000 description 1
• 238000002073 fluorescence micrograph Methods 0.000 description 1
• 239000007850 fluorescent dye Substances 0.000 description 1
• 238000013467 fragmentation Methods 0.000 description 1
• 238000006062 fragmentation reaction Methods 0.000 description 1
• 210000002064 heart cell Anatomy 0.000 description 1
• 238000010438 heat treatment Methods 0.000 description 1
• 229920001519 homopolymer Polymers 0.000 description 1
• 239000000017 hydrogel Substances 0.000 description 1
• 238000011532 immunohistochemical staining Methods 0.000 description 1
• 230000008774 maternal effect Effects 0.000 description 1
• 239000011159 matrix material Substances 0.000 description 1
• 230000001394 metastastic effect Effects 0.000 description 1
• 206010061289 metastatic neoplasm Diseases 0.000 description 1
• 210000000056 organ Anatomy 0.000 description 1
• 230000008775 paternal effect Effects 0.000 description 1
• 229920000642 polymer Polymers 0.000 description 1
• 230000000750 progressive effect Effects 0.000 description 1
• 239000004094 surface-active agent Substances 0.000 description 1
• 238000012070 whole genome sequencing analysis Methods 0.000 description 1