JP2018509896A5 - - Google Patents
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- JP2018509896A5 JP2018509896A5 JP2017542907A JP2017542907A JP2018509896A5 JP 2018509896 A5 JP2018509896 A5 JP 2018509896A5 JP 2017542907 A JP2017542907 A JP 2017542907A JP 2017542907 A JP2017542907 A JP 2017542907A JP 2018509896 A5 JP2018509896 A5 JP 2018509896A5
- Authority
- JP
- Japan
- Prior art keywords
- linker
- barcode
- umi
- capture
- nucleotide sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 239000003153 chemical reaction reagent Substances 0.000 claims 24
- 238000000034 method Methods 0.000 claims 24
- 239000002773 nucleotide Substances 0.000 claims 20
- 239000007795 chemical reaction product Substances 0.000 claims 16
- 150000007523 nucleic acids Chemical group 0.000 claims 12
- 230000001413 cellular effect Effects 0.000 claims 10
- 238000006243 chemical reaction Methods 0.000 claims 9
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 6
- 125000003729 nucleotide group Chemical group 0.000 claims 5
- 238000010839 reverse transcription Methods 0.000 claims 5
- 230000003321 amplification Effects 0.000 claims 4
- 239000011159 matrix material Substances 0.000 claims 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 108091034117 Oligonucleotide Proteins 0.000 claims 3
- 239000002299 complementary DNA Substances 0.000 claims 2
- 238000012800 visualization Methods 0.000 claims 2
- 230000004544 DNA amplification Effects 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000010348 incorporation Methods 0.000 claims 1
- 238000011084 recovery Methods 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562126349P | 2015-02-27 | 2015-02-27 | |
| US62/126,349 | 2015-02-27 | ||
| PCT/US2016/019952 WO2016138490A1 (en) | 2015-02-27 | 2016-02-26 | Single-cell nucleic acids for high-throughput studies |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2018509896A JP2018509896A (ja) | 2018-04-12 |
| JP2018509896A5 true JP2018509896A5 (enExample) | 2019-04-04 |
Family
ID=56789110
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017542907A Pending JP2018509896A (ja) | 2015-02-27 | 2016-02-26 | 高処理能力研究用の単一細胞核酸 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10190163B2 (enExample) |
| EP (1) | EP3262214B1 (enExample) |
| JP (1) | JP2018509896A (enExample) |
| CN (1) | CN107250445A (enExample) |
| CA (1) | CA2976681A1 (enExample) |
| SG (1) | SG11201706636PA (enExample) |
| WO (1) | WO2016138490A1 (enExample) |
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| US9707562B2 (en) | 2013-03-13 | 2017-07-18 | Denovo Sciences, Inc. | System for capturing and analyzing cells |
| US10391490B2 (en) | 2013-05-31 | 2019-08-27 | Celsee Diagnostics, Inc. | System and method for isolating and analyzing cells |
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| WO2017079593A1 (en) * | 2015-11-04 | 2017-05-11 | Atreca, Inc. | Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells |
| WO2017106777A1 (en) | 2015-12-16 | 2017-06-22 | Fluidigm Corporation | High-level multiplex amplification |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| IL266197B2 (en) | 2016-10-24 | 2024-03-01 | Geneinfosec Inc | Hiding information contained within nucleic acids |
| CN110520541B (zh) * | 2017-02-13 | 2024-03-26 | 耶鲁大学 | 高通量单细胞多组学 |
| CA3059559A1 (en) | 2017-06-05 | 2018-12-13 | Becton, Dickinson And Company | Sample indexing for single cells |
| AU2018288849B2 (en) * | 2017-06-20 | 2024-08-22 | Psomagen, Inc | Method and system for library preparation with unique molecular identifiers |
| US10391493B2 (en) | 2017-08-29 | 2019-08-27 | Celsee Diagnostics, Inc. | System and method for isolating and analyzing cells |
| CN111094587B (zh) * | 2017-08-30 | 2024-08-06 | 卡帕生物系统公司 | 转座酶组合物、制备方法和筛选方法 |
| EP3755799A4 (en) * | 2018-02-23 | 2021-12-08 | Yale University | LYSIS BY FREEZE-DEFROST OF A SINGLE CELL |
| EP3788171B1 (en) | 2018-05-03 | 2023-04-05 | Becton, Dickinson and Company | High throughput multiomics sample analysis |
| WO2020036926A1 (en) | 2018-08-17 | 2020-02-20 | Cellecta, Inc. | Multiplex preparation of barcoded gene specific dna fragments |
| CN112805389B (zh) * | 2018-10-01 | 2024-08-06 | 贝克顿迪金森公司 | 确定5’转录物序列 |
| US12071617B2 (en) | 2019-02-14 | 2024-08-27 | Becton, Dickinson And Company | Hybrid targeted and whole transcriptome amplification |
| US10633693B1 (en) | 2019-04-16 | 2020-04-28 | Celsee Diagnostics, Inc. | System and method for leakage control in a particle capture system |
| US11273439B2 (en) | 2019-05-07 | 2022-03-15 | Bio-Rad Laboratories, Inc. | System and method for target material retrieval from microwells |
| US11578322B2 (en) | 2019-05-07 | 2023-02-14 | Bio-Rad Laboratories, Inc. | System and method for automated single cell processing |
| EP3982716A4 (en) | 2019-06-14 | 2023-09-06 | Bio-Rad Laboratories, Inc. | System and method for automated single cell processing and analyses |
| US20220333194A1 (en) * | 2019-08-08 | 2022-10-20 | Inserm (Institutnationaldelasanteetdelarecherchemedicale) | Rna sequencing method for the analysis of b and t cell transcriptome in phenotypically defined b and t cell subsets |
| EP4025709A1 (en) * | 2019-09-06 | 2022-07-13 | 10X Genomics, Inc. | Systems and methods for barcoding cells and cell beads |
| CN114555824A (zh) * | 2019-10-11 | 2022-05-27 | 布莱克霍克基因组学有限责任公司 | 使用长读测序鉴定基因组结构变体 |
| GB2589869A (en) * | 2019-12-09 | 2021-06-16 | Univ Oxford Innovation Ltd | Method for whole genome sequencing of picogram quantities of DNA |
| US12445148B2 (en) * | 2020-01-03 | 2025-10-14 | Koninklijke Philips N.V. | System and method for effective compression representation and decompression of diverse tabulated data |
| CN115335520A (zh) | 2020-01-29 | 2022-11-11 | 贝克顿迪金森公司 | 用于通过测序对单细胞进行空间映射的条形码化的孔 |
| US12153043B2 (en) | 2020-02-25 | 2024-11-26 | Becton, Dickinson And Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| US11504719B2 (en) | 2020-03-12 | 2022-11-22 | Bio-Rad Laboratories, Inc. | System and method for receiving and delivering a fluid for sample processing |
| US11661625B2 (en) | 2020-05-14 | 2023-05-30 | Becton, Dickinson And Company | Primers for immune repertoire profiling |
| EP4407030B1 (en) | 2020-06-02 | 2025-12-17 | Becton, Dickinson and Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| US20230399693A1 (en) * | 2020-11-03 | 2023-12-14 | Act Genomics (Ip) Co., Limited | Targeted sequencing method and kit thereof for detecting gene alteration |
| US12392771B2 (en) | 2020-12-15 | 2025-08-19 | Becton, Dickinson And Company | Single cell secretome analysis |
| WO2023037334A1 (en) | 2021-09-13 | 2023-03-16 | Ecole Polytechnique Federale De Lausanne (Epfl) | System and method for single cell phenotypical profiling and deterministic nanoliter-droplet encapsulation and deterministic droplet consortia assemblies |
| CN118369438A (zh) | 2021-12-10 | 2024-07-19 | 伯乐实验室有限公司 | 用形态可调节功能化颗粒进行样品处理的组合物、方法和系统 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US5449602A (en) | 1988-01-13 | 1995-09-12 | Amoco Corporation | Template-directed photoligation |
| US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
| EP2573101B1 (en) | 1996-02-09 | 2016-04-20 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| WO1997045559A1 (en) | 1996-05-29 | 1997-12-04 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| US6312892B1 (en) | 1996-07-19 | 2001-11-06 | Cornell Research Foundation, Inc. | High fidelity detection of nucleic acid differences by ligase detection reaction |
| US6770748B2 (en) | 1997-03-07 | 2004-08-03 | Takeshi Imanishi | Bicyclonucleoside and oligonucleotide analogue |
| US6794499B2 (en) | 1997-09-12 | 2004-09-21 | Exiqon A/S | Oligonucleotide analogues |
| US6027998A (en) | 1997-12-17 | 2000-02-22 | Advanced Micro Devices, Inc. | Method for fully planarized conductive line for a stack gate |
| US6506594B1 (en) | 1999-03-19 | 2003-01-14 | Cornell Res Foundation Inc | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| US6262490B1 (en) | 1999-11-05 | 2001-07-17 | Advanced Semiconductor Engineering, Inc. | Substrate strip for use in packaging semiconductor chips |
| JP2004507226A (ja) | 2000-05-30 | 2004-03-11 | ピーイー コーポレイション （エヌワイ） | ライゲーションおよび増幅の組み合わせを用いて標的核酸を検出するための方法 |
| US6605451B1 (en) | 2000-06-06 | 2003-08-12 | Xtrana, Inc. | Methods and devices for multiplexing amplification reactions |
| EP1334347A1 (en) | 2000-09-15 | 2003-08-13 | California Institute Of Technology | Microfabricated crossflow devices and methods |
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| CN102015522B (zh) | 2008-02-22 | 2013-07-03 | 弗卢迪格姆公司 | 用于微流体器件的集成载体 |
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| CN102348811A (zh) | 2009-01-13 | 2012-02-08 | 弗卢伊蒂格姆公司 | 单细胞核酸分析 |
| WO2010088288A2 (en) | 2009-01-28 | 2010-08-05 | Fluidigm Corporation | Determination of copy number differences by amplification |
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| EP3495498B1 (en) * | 2009-03-30 | 2021-10-27 | Illumina, Inc. | Gene expression analysis in single cells |
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| EP2569447A4 (en) | 2010-05-14 | 2013-11-27 | Fluidigm Corp | ASSAYS FOR THE DETECTION OF GENOTYPES, MUTATIONS OR ANEUPLOIDY |
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| WO2012162267A2 (en) * | 2011-05-20 | 2012-11-29 | Fluidigm Corporation | Nucleic acid encoding reactions |
| US9506845B2 (en) * | 2012-02-29 | 2016-11-29 | Fluidigm Corporation | Methods, systems and devices for multiple single-cell capturing and processing using microfluidics |
| CN104471077B (zh) | 2012-05-21 | 2017-05-24 | 富鲁达公司 | 颗粒群的单颗粒分析 |
| WO2014031954A1 (en) * | 2012-08-23 | 2014-02-27 | Tufts University | Homopolymer mediated nucleic acid amplification |
| US20160122753A1 (en) * | 2013-06-12 | 2016-05-05 | Tarjei Mikkelsen | High-throughput rna-seq |
| GB201317301D0 (en) | 2013-09-30 | 2013-11-13 | Linnarsson Sten | Method for capturing and encoding nucleic acid from a plurality of single cells |
| EP3262214B1 (en) | 2015-02-27 | 2023-10-25 | Standard BioTools Inc. | Microfluidic device |
| WO2017106777A1 (en) | 2015-12-16 | 2017-06-22 | Fluidigm Corporation | High-level multiplex amplification |
-
2016
- 2016-02-26 EP EP16756523.3A patent/EP3262214B1/en active Active
- 2016-02-26 JP JP2017542907A patent/JP2018509896A/ja active Pending
- 2016-02-26 CN CN201680012542.8A patent/CN107250445A/zh active Pending
- 2016-02-26 CA CA2976681A patent/CA2976681A1/en active Pending
- 2016-02-26 WO PCT/US2016/019952 patent/WO2016138490A1/en not_active Ceased
- 2016-02-26 US US15/055,252 patent/US10190163B2/en active Active
- 2016-02-26 SG SG11201706636PA patent/SG11201706636PA/en unknown
-
2018
- 2018-12-10 US US16/215,489 patent/US10954560B2/en active Active
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