JP2018509896A5 - - Google Patents
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- JP2018509896A5 JP2018509896A5 JP2017542907A JP2017542907A JP2018509896A5 JP 2018509896 A5 JP2018509896 A5 JP 2018509896A5 JP 2017542907 A JP2017542907 A JP 2017542907A JP 2017542907 A JP2017542907 A JP 2017542907A JP 2018509896 A5 JP2018509896 A5 JP 2018509896A5
- Authority
- JP
- Japan
- Prior art keywords
- linker
- barcode
- umi
- capture
- nucleotide sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 239000003153 chemical reaction reagent Substances 0.000 claims 24
- 238000000034 method Methods 0.000 claims 24
- 239000002773 nucleotide Substances 0.000 claims 20
- 239000007795 chemical reaction product Substances 0.000 claims 16
- 150000007523 nucleic acids Chemical group 0.000 claims 12
- 230000001413 cellular effect Effects 0.000 claims 10
- 238000006243 chemical reaction Methods 0.000 claims 9
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 6
- 125000003729 nucleotide group Chemical group 0.000 claims 5
- 238000010839 reverse transcription Methods 0.000 claims 5
- 230000003321 amplification Effects 0.000 claims 4
- 239000011159 matrix material Substances 0.000 claims 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 108091034117 Oligonucleotide Proteins 0.000 claims 3
- 239000002299 complementary DNA Substances 0.000 claims 2
- 238000012800 visualization Methods 0.000 claims 2
- 230000004544 DNA amplification Effects 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000010348 incorporation Methods 0.000 claims 1
- 238000011084 recovery Methods 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562126349P | 2015-02-27 | 2015-02-27 | |
| US62/126,349 | 2015-02-27 | ||
| PCT/US2016/019952 WO2016138490A1 (en) | 2015-02-27 | 2016-02-26 | Single-cell nucleic acids for high-throughput studies |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2018509896A JP2018509896A (ja) | 2018-04-12 |
| JP2018509896A5 true JP2018509896A5 (enExample) | 2019-04-04 |
Family
ID=56789110
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017542907A Pending JP2018509896A (ja) | 2015-02-27 | 2016-02-26 | 高処理能力研究用の単一細胞核酸 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10190163B2 (enExample) |
| EP (1) | EP3262214B1 (enExample) |
| JP (1) | JP2018509896A (enExample) |
| CN (1) | CN107250445A (enExample) |
| CA (1) | CA2976681A1 (enExample) |
| SG (1) | SG11201706636PA (enExample) |
| WO (1) | WO2016138490A1 (enExample) |
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| CN111295245B (zh) | 2017-08-29 | 2021-03-16 | 伯乐实验室有限公司 | 用于分离和分析细胞的系统和方法 |
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| EP3755799A4 (en) * | 2018-02-23 | 2021-12-08 | Yale University | LYSIS BY FREEZE-DEFROST OF A SINGLE CELL |
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| WO2020036926A1 (en) | 2018-08-17 | 2020-02-20 | Cellecta, Inc. | Multiplex preparation of barcoded gene specific dna fragments |
| EP3861134B1 (en) * | 2018-10-01 | 2024-09-04 | Becton, Dickinson and Company | Determining 5' transcript sequences |
| CN113454234B (zh) | 2019-02-14 | 2025-03-18 | 贝克顿迪金森公司 | 杂合体靶向和全转录物组扩增 |
| US10633693B1 (en) | 2019-04-16 | 2020-04-28 | Celsee Diagnostics, Inc. | System and method for leakage control in a particle capture system |
| JP7413408B2 (ja) | 2019-05-07 | 2024-01-15 | バイオ－ラッド ラボラトリーズ インコーポレイテッド | 自動化された単一細胞処理のためのシステムおよび方法 |
| US11273439B2 (en) | 2019-05-07 | 2022-03-15 | Bio-Rad Laboratories, Inc. | System and method for target material retrieval from microwells |
| CN118291246A (zh) | 2019-06-14 | 2024-07-05 | 伯乐实验室有限公司 | 用于自动化单细胞处理和分析的系统和方法 |
| JP2022544101A (ja) * | 2019-08-08 | 2022-10-17 | アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル | 表現型の規定されたｂ細胞及びｔ細胞のサブセットにおける、ｂ細胞とｔ細胞のトランスクリプトームの分析のためのｒｎａシークエンス法 |
| WO2021046475A1 (en) * | 2019-09-06 | 2021-03-11 | 10X Genomics, Inc. | Systems and methods for barcoding cells and cell beads |
| EP4041916A4 (en) * | 2019-10-11 | 2023-11-01 | Blackhawk Genomics LLC | IDENTIFICATION OF GENOMIC STRUCTURAL VARIANTS USING LONG READ SEQUENCING |
| EP4055160B1 (en) | 2019-11-08 | 2024-04-10 | Becton Dickinson and Company | Using random priming to obtain full-length v(d)j information for immune repertoire sequencing |
| GB2589869A (en) * | 2019-12-09 | 2021-06-16 | Univ Oxford Innovation Ltd | Method for whole genome sequencing of picogram quantities of DNA |
| US12445148B2 (en) * | 2020-01-03 | 2025-10-14 | Koninklijke Philips N.V. | System and method for effective compression representation and decompression of diverse tabulated data |
| CN115279919A (zh) | 2020-01-13 | 2022-11-01 | 贝克顿迪金森公司 | 使用含有du的寡核苷酸的细胞捕获 |
| WO2021155057A1 (en) | 2020-01-29 | 2021-08-05 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| US12153043B2 (en) | 2020-02-25 | 2024-11-26 | Becton, Dickinson And Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
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| CN115803445A (zh) | 2020-06-02 | 2023-03-14 | 贝克顿迪金森公司 | 用于5撇基因表达测定的寡核苷酸和珠 |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| JP2023547732A (ja) * | 2020-11-03 | 2023-11-13 | エイシーティ・ジェノミックス（アイピー）リミテッド | 遺伝子改変を検出するための標的化シークエンシング方法およびそのキット |
| CN116685850A (zh) | 2020-12-15 | 2023-09-01 | 贝克顿迪金森公司 | 单细胞分泌组分析 |
| US20240401026A1 (en) | 2021-09-13 | 2024-12-05 | Ecole Polytechnique Federale De Lausanne (Epfl) | System and method for single cell phenotypical profiling and deterministic nanoliter-droplet encapsulation and deterministic droplet consortia assemblies |
| WO2023107539A1 (en) | 2021-12-10 | 2023-06-15 | Bio-Rad Laboratories, Inc. | Compositions, methods, and systems for sample processing with morphology-adjustable functionalized particles |
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| US5449602A (en) | 1988-01-13 | 1995-09-12 | Amoco Corporation | Template-directed photoligation |
| US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
| AU735440B2 (en) | 1996-02-09 | 2001-07-05 | Cornell Research Foundation Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| AU730633B2 (en) | 1996-05-29 | 2001-03-08 | Phillip Belgrader | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| US6312892B1 (en) | 1996-07-19 | 2001-11-06 | Cornell Research Foundation, Inc. | High fidelity detection of nucleic acid differences by ligase detection reaction |
| US6770748B2 (en) | 1997-03-07 | 2004-08-03 | Takeshi Imanishi | Bicyclonucleoside and oligonucleotide analogue |
| US6794499B2 (en) | 1997-09-12 | 2004-09-21 | Exiqon A/S | Oligonucleotide analogues |
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| WO2014031954A1 (en) * | 2012-08-23 | 2014-02-27 | Tufts University | Homopolymer mediated nucleic acid amplification |
| US20160122753A1 (en) * | 2013-06-12 | 2016-05-05 | Tarjei Mikkelsen | High-throughput rna-seq |
| GB201317301D0 (en) | 2013-09-30 | 2013-11-13 | Linnarsson Sten | Method for capturing and encoding nucleic acid from a plurality of single cells |
| CN107250445A (zh) | 2015-02-27 | 2017-10-13 | 富鲁达公司 | 用于高通量研究的单个细胞核酸 |
| CA3006994A1 (en) | 2015-12-16 | 2017-06-22 | Fluidigm Corporation | High-level multiplex amplification |
-
2016
- 2016-02-26 CN CN201680012542.8A patent/CN107250445A/zh active Pending
- 2016-02-26 JP JP2017542907A patent/JP2018509896A/ja active Pending
- 2016-02-26 CA CA2976681A patent/CA2976681A1/en active Pending
- 2016-02-26 SG SG11201706636PA patent/SG11201706636PA/en unknown
- 2016-02-26 EP EP16756523.3A patent/EP3262214B1/en active Active
- 2016-02-26 WO PCT/US2016/019952 patent/WO2016138490A1/en not_active Ceased
- 2016-02-26 US US15/055,252 patent/US10190163B2/en active Active
-
2018
- 2018-12-10 US US16/215,489 patent/US10954560B2/en active Active
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