JP2018509896A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2018509896A5 JP2018509896A5 JP2017542907A JP2017542907A JP2018509896A5 JP 2018509896 A5 JP2018509896 A5 JP 2018509896A5 JP 2017542907 A JP2017542907 A JP 2017542907A JP 2017542907 A JP2017542907 A JP 2017542907A JP 2018509896 A5 JP2018509896 A5 JP 2018509896A5
- Authority
- JP
- Japan
- Prior art keywords
- linker
- barcode
- umi
- capture
- nucleotide sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003153 chemical reaction reagent Substances 0.000 claims 24
- 238000000034 method Methods 0.000 claims 24
- 239000002773 nucleotide Substances 0.000 claims 20
- 239000007795 chemical reaction product Substances 0.000 claims 16
- 150000007523 nucleic acids Chemical group 0.000 claims 12
- 230000001413 cellular effect Effects 0.000 claims 10
- 238000006243 chemical reaction Methods 0.000 claims 9
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 6
- 125000003729 nucleotide group Chemical group 0.000 claims 5
- 238000010839 reverse transcription Methods 0.000 claims 5
- 230000003321 amplification Effects 0.000 claims 4
- 239000011159 matrix material Substances 0.000 claims 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 108091034117 Oligonucleotide Proteins 0.000 claims 3
- 239000002299 complementary DNA Substances 0.000 claims 2
- 238000012800 visualization Methods 0.000 claims 2
- 230000004544 DNA amplification Effects 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000010348 incorporation Methods 0.000 claims 1
- 238000011084 recovery Methods 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562126349P | 2015-02-27 | 2015-02-27 | |
| US62/126,349 | 2015-02-27 | ||
| PCT/US2016/019952 WO2016138490A1 (en) | 2015-02-27 | 2016-02-26 | Single-cell nucleic acids for high-throughput studies |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2018509896A JP2018509896A (ja) | 2018-04-12 |
| JP2018509896A5 true JP2018509896A5 (enExample) | 2019-04-04 |
Family
ID=56789110
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017542907A Pending JP2018509896A (ja) | 2015-02-27 | 2016-02-26 | 高処理能力研究用の単一細胞核酸 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10190163B2 (enExample) |
| EP (1) | EP3262214B1 (enExample) |
| JP (1) | JP2018509896A (enExample) |
| CN (1) | CN107250445A (enExample) |
| CA (1) | CA2976681A1 (enExample) |
| SG (1) | SG11201706636PA (enExample) |
| WO (1) | WO2016138490A1 (enExample) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2789694A1 (en) | 2009-04-02 | 2014-10-15 | Fluidigm Corporation | Microfluidic device with reaction product recovery system |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US9074204B2 (en) | 2011-05-20 | 2015-07-07 | Fluidigm Corporation | Nucleic acid encoding reactions |
| ES2797448T3 (es) | 2011-08-01 | 2020-12-02 | Bio Rad Laboratories | Sistema de captura de células |
| US9404864B2 (en) | 2013-03-13 | 2016-08-02 | Denovo Sciences, Inc. | System for imaging captured cells |
| US10466160B2 (en) | 2011-08-01 | 2019-11-05 | Celsee Diagnostics, Inc. | System and method for retrieving and analyzing particles |
| US9752181B2 (en) | 2013-01-26 | 2017-09-05 | Denovo Sciences, Inc. | System and method for capturing and analyzing cells |
| US9707562B2 (en) | 2013-03-13 | 2017-07-18 | Denovo Sciences, Inc. | System for capturing and analyzing cells |
| US10391490B2 (en) | 2013-05-31 | 2019-08-27 | Celsee Diagnostics, Inc. | System and method for isolating and analyzing cells |
| US9856535B2 (en) | 2013-05-31 | 2018-01-02 | Denovo Sciences, Inc. | System for isolating cells |
| US10190163B2 (en) | 2015-02-27 | 2019-01-29 | Fluidigm Corporation | Single cell nucleic acids for high-throughput studies |
| JP7064439B2 (ja) * | 2015-11-04 | 2022-05-10 | アトレカ インコーポレイテッド | 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット |
| US11117113B2 (en) | 2015-12-16 | 2021-09-14 | Fluidigm Corporation | High-level multiplex amplification |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| ES2870639T3 (es) | 2016-10-24 | 2021-10-27 | Geneinfosec Inc | Ocultación de información presente en los ácidos nucleicos |
| US11753743B2 (en) | 2017-02-13 | 2023-09-12 | Yale University | High-throughput single-cell polyomics |
| WO2018226293A1 (en) | 2017-06-05 | 2018-12-13 | Becton, Dickinson And Company | Sample indexing for single cells |
| WO2018237092A1 (en) * | 2017-06-20 | 2018-12-27 | uBiome, Inc. | Method and system for library preparation with unique molecular identifiers |
| CN111295245B (zh) | 2017-08-29 | 2021-03-16 | 伯乐实验室有限公司 | 用于分离和分析细胞的系统和方法 |
| US20200347441A1 (en) * | 2017-08-30 | 2020-11-05 | Kapa Biosystems, Inc. | Transposase compositions, methods of making, and methods of screening |
| US20210087549A1 (en) * | 2018-02-23 | 2021-03-25 | Yale University | Single-cell freeze-thaw lysis |
| WO2019213294A1 (en) | 2018-05-03 | 2019-11-07 | Becton, Dickinson And Company | High throughput multiomics sample analysis |
| WO2020036926A1 (en) * | 2018-08-17 | 2020-02-20 | Cellecta, Inc. | Multiplex preparation of barcoded gene specific dna fragments |
| CN118853827A (zh) * | 2018-10-01 | 2024-10-29 | 贝克顿迪金森公司 | 确定5’转录物序列 |
| EP3924506A1 (en) | 2019-02-14 | 2021-12-22 | Becton Dickinson and Company | Hybrid targeted and whole transcriptome amplification |
| US10633693B1 (en) | 2019-04-16 | 2020-04-28 | Celsee Diagnostics, Inc. | System and method for leakage control in a particle capture system |
| US11273439B2 (en) | 2019-05-07 | 2022-03-15 | Bio-Rad Laboratories, Inc. | System and method for target material retrieval from microwells |
| JP7413408B2 (ja) | 2019-05-07 | 2024-01-15 | バイオ－ラッド ラボラトリーズ インコーポレイテッド | 自動化された単一細胞処理のためのシステムおよび方法 |
| JP7356519B2 (ja) | 2019-06-14 | 2023-10-04 | バイオ－ラッド ラボラトリーズ インコーポレイテッド | 自動化された単一細胞処理及び解析のためのシステム及び方法 |
| WO2021023853A1 (en) * | 2019-08-08 | 2021-02-11 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Rna sequencing method for the analysis of b and t cell transcriptome in phenotypically defined b and t cell subsets |
| EP4025709A1 (en) * | 2019-09-06 | 2022-07-13 | 10X Genomics, Inc. | Systems and methods for barcoding cells and cell beads |
| CN114555824A (zh) * | 2019-10-11 | 2022-05-27 | 布莱克霍克基因组学有限责任公司 | 使用长读测序鉴定基因组结构变体 |
| GB2589869A (en) * | 2019-12-09 | 2021-06-16 | Univ Oxford Innovation Ltd | Method for whole genome sequencing of picogram quantities of DNA |
| US12445148B2 (en) * | 2020-01-03 | 2025-10-14 | Koninklijke Philips N.V. | System and method for effective compression representation and decompression of diverse tabulated data |
| ES2993319T3 (en) | 2020-01-29 | 2024-12-27 | Becton Dickinson Co | Barcoded wells for spatial mapping of single cells through sequencing |
| EP4111168A1 (en) | 2020-02-25 | 2023-01-04 | Becton Dickinson and Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| US11504719B2 (en) | 2020-03-12 | 2022-11-22 | Bio-Rad Laboratories, Inc. | System and method for receiving and delivering a fluid for sample processing |
| CN115605614A (zh) | 2020-05-14 | 2023-01-13 | 贝克顿迪金森公司（Us） | 用于免疫组库谱分析的引物 |
| WO2021247593A1 (en) | 2020-06-02 | 2021-12-09 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| US20230399693A1 (en) * | 2020-11-03 | 2023-12-14 | Act Genomics (Ip) Co., Limited | Targeted sequencing method and kit thereof for detecting gene alteration |
| EP4263859A1 (en) | 2020-12-15 | 2023-10-25 | Becton, Dickinson and Company | Single cell secretome analysis |
| US20240401026A1 (en) | 2021-09-13 | 2024-12-05 | Ecole Polytechnique Federale De Lausanne (Epfl) | System and method for single cell phenotypical profiling and deterministic nanoliter-droplet encapsulation and deterministic droplet consortia assemblies |
| EP4444910A4 (en) | 2021-12-10 | 2025-11-05 | Bio Rad Laboratories Inc | Compositions, processes and systems for the treatment of samples with functionalized particles of adjustable morphology |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5449602A (en) | 1988-01-13 | 1995-09-12 | Amoco Corporation | Template-directed photoligation |
| US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
| CA2244891C (en) | 1996-02-09 | 2008-12-30 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| JP4468488B2 (ja) | 1996-05-29 | 2010-05-26 | コーネル・リサーチ・ファンデーション・インコーポレイテッド | 組み合せたリガーゼ検出およびポリメラーゼ連鎖反応を用いる核酸配列相違の検出 |
| US6312892B1 (en) | 1996-07-19 | 2001-11-06 | Cornell Research Foundation, Inc. | High fidelity detection of nucleic acid differences by ligase detection reaction |
| US6770748B2 (en) | 1997-03-07 | 2004-08-03 | Takeshi Imanishi | Bicyclonucleoside and oligonucleotide analogue |
| US6794499B2 (en) | 1997-09-12 | 2004-09-21 | Exiqon A/S | Oligonucleotide analogues |
| US6027998A (en) | 1997-12-17 | 2000-02-22 | Advanced Micro Devices, Inc. | Method for fully planarized conductive line for a stack gate |
| US6506594B1 (en) | 1999-03-19 | 2003-01-14 | Cornell Res Foundation Inc | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| US6262490B1 (en) | 1999-11-05 | 2001-07-17 | Advanced Semiconductor Engineering, Inc. | Substrate strip for use in packaging semiconductor chips |
| WO2001092579A2 (en) | 2000-05-30 | 2001-12-06 | Pe Corporation (Ny) | Methods for detecting target nucleic acids using coupled ligation and amplification |
| US6605451B1 (en) | 2000-06-06 | 2003-08-12 | Xtrana, Inc. | Methods and devices for multiplexing amplification reactions |
| EP1334347A1 (en) | 2000-09-15 | 2003-08-13 | California Institute Of Technology | Microfabricated crossflow devices and methods |
| US20100022414A1 (en) | 2008-07-18 | 2010-01-28 | Raindance Technologies, Inc. | Droplet Libraries |
| EP2477029A1 (en) | 2005-06-02 | 2012-07-18 | Fluidigm Corporation | Analysis using microfluidic partitioning devices |
| US9440231B2 (en) | 2007-08-14 | 2016-09-13 | Fluidigm Corporation | Polymer microfluidic biochip fabrication |
| WO2009105609A1 (en) | 2008-02-22 | 2009-08-27 | Fluidigm Corporation | Integrated carrier for microfluidic device |
| WO2010027870A2 (en) | 2008-08-26 | 2010-03-11 | Fluidigm Corporation | Assay methods for increased throughput of samples and/or targets |
| US8628923B2 (en) | 2009-01-13 | 2014-01-14 | Fluidigm Corporation | Single cell nucleic acid analysis |
| US8450063B2 (en) | 2009-01-28 | 2013-05-28 | Fluidigm Corporation | Determination of copy number differences by amplification |
| US8528589B2 (en) | 2009-03-23 | 2013-09-10 | Raindance Technologies, Inc. | Manipulation of microfluidic droplets |
| ES2706227T3 (es) * | 2009-03-30 | 2019-03-27 | Illumina Inc | Análisis de expresión génica en células individuales |
| EP2789694A1 (en) | 2009-04-02 | 2014-10-15 | Fluidigm Corporation | Microfluidic device with reaction product recovery system |
| US20100285537A1 (en) | 2009-04-02 | 2010-11-11 | Fluidigm Corporation | Selective tagging of short nucleic acid fragments and selective protection of target sequences from degradation |
| US20140186827A1 (en) | 2010-05-14 | 2014-07-03 | Fluidigm, Inc. | Assays for the detection of genotype, mutations, and/or aneuploidy |
| WO2011143659A2 (en) | 2010-05-14 | 2011-11-17 | Fluidigm Corporation | Nucleic acid isolation methods |
| US9074204B2 (en) | 2011-05-20 | 2015-07-07 | Fluidigm Corporation | Nucleic acid encoding reactions |
| US20130302884A1 (en) * | 2012-02-29 | 2013-11-14 | Fluidigm Corporation | Methods, systems and devices for multiple single-cell capturing and processing using microfluidics |
| JP2015519900A (ja) | 2012-05-21 | 2015-07-16 | フリューダイム・コーポレイション | 粒子集団の単粒子解析方法及び単粒子単離方法 |
| US9914950B2 (en) * | 2012-08-23 | 2018-03-13 | Tufts University | Homopolymer mediated nucleic acid amplification |
| US20160122753A1 (en) * | 2013-06-12 | 2016-05-05 | Tarjei Mikkelsen | High-throughput rna-seq |
| GB201317301D0 (en) | 2013-09-30 | 2013-11-13 | Linnarsson Sten | Method for capturing and encoding nucleic acid from a plurality of single cells |
| US10190163B2 (en) | 2015-02-27 | 2019-01-29 | Fluidigm Corporation | Single cell nucleic acids for high-throughput studies |
| US11117113B2 (en) | 2015-12-16 | 2021-09-14 | Fluidigm Corporation | High-level multiplex amplification |
-
2016
- 2016-02-26 US US15/055,252 patent/US10190163B2/en active Active
- 2016-02-26 SG SG11201706636PA patent/SG11201706636PA/en unknown
- 2016-02-26 WO PCT/US2016/019952 patent/WO2016138490A1/en not_active Ceased
- 2016-02-26 CA CA2976681A patent/CA2976681A1/en active Pending
- 2016-02-26 EP EP16756523.3A patent/EP3262214B1/en active Active
- 2016-02-26 CN CN201680012542.8A patent/CN107250445A/zh active Pending
- 2016-02-26 JP JP2017542907A patent/JP2018509896A/ja active Pending
-
2018
- 2018-12-10 US US16/215,489 patent/US10954560B2/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2018509896A5 (enExample) | ||
| JP2019528762A5 (enExample) | ||
| EP3253491B1 (en) | System and method for single cell genetic analysis | |
| HRP20161056T1 (hr) | Metode za odabir i umnožavanje polinukleotida | |
| JP2017532042A5 (enExample) | ||
| JP2014504153A5 (enExample) | ||
| US20150050657A1 (en) | Targeted enrichment and amplification of nucleic acids on a support | |
| US20160251712A1 (en) | Sequential Sequencing | |
| US20170058342A1 (en) | Materials and methods to analyze rna isoforms in transcriptomes | |
| JP2020523034A5 (enExample) | ||
| CN105861487A (zh) | 用于靶向核酸序列富集和高效文库产生的组合物和方法 | |
| EP2531610B1 (en) | Complexitiy reduction method | |
| CN108603224B (zh) | 高水平多路复用扩增 | |
| HRP20201744T1 (hr) | Novi postupci proizvodnje oligonukleotida | |
| WO2004039953A3 (en) | Array oligomer synthesis and use. | |
| JP2018514230A5 (enExample) | ||
| JP2011507488A5 (enExample) | ||
| JP2019523652A5 (enExample) | ||
| JP2019514360A (ja) | 超並列シークエンシングのためのｄｎａライブラリーを生成する方法及びキット | |
| KR101600899B1 (ko) | 초병렬적 핵산 합성법을 통한 dna 라이브러리의 동시 합성 방법 | |
| JP2023004952A5 (enExample) | ||
| JP2018518968A5 (enExample) | ||
| WO2019116800A1 (ja) | 単一細胞の網羅的３'末端遺伝子発現解析法 | |
| Tang et al. | New tools for cost-effective DNA synthesis | |
| CN101942431A (zh) | 从生物样品中选择性捕获和扩增外显子或靶标基因组区域的方法 |