JP2020523034A5 - - Google Patents
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- JP2020523034A5 JP2020523034A5 JP2020515835A JP2020515835A JP2020523034A5 JP 2020523034 A5 JP2020523034 A5 JP 2020523034A5 JP 2020515835 A JP2020515835 A JP 2020515835A JP 2020515835 A JP2020515835 A JP 2020515835A JP 2020523034 A5 JP2020523034 A5 JP 2020523034A5
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- JP
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- Prior art keywords
- polynucleotide
- polynucleotides
- barcoded
- vessel
- adapter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108091033319 polynucleotide Proteins 0.000 claims description 411
- 239000002157 polynucleotide Substances 0.000 claims description 411
- 102000040430 polynucleotide Human genes 0.000 claims description 411
- 238000000034 method Methods 0.000 claims description 208
- 210000004027 cell Anatomy 0.000 claims description 108
- 230000000295 complement effect Effects 0.000 claims description 90
- 108091034117 Oligonucleotide Proteins 0.000 claims description 89
- 239000013615 primer Substances 0.000 claims description 77
- 230000037452 priming Effects 0.000 claims description 59
- 125000003729 nucleotide group Chemical group 0.000 claims description 46
- 239000002773 nucleotide Substances 0.000 claims description 45
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 36
- 108091093088 Amplicon Proteins 0.000 claims description 20
- 108091008874 T cell receptors Proteins 0.000 claims description 18
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 claims description 18
- 102100034343 Integrase Human genes 0.000 claims description 17
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 17
- 238000012163 sequencing technique Methods 0.000 claims description 17
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 16
- 108060003951 Immunoglobulin Proteins 0.000 claims description 15
- 230000003321 amplification Effects 0.000 claims description 15
- 102000018358 immunoglobulin Human genes 0.000 claims description 15
- 108020004635 Complementary DNA Proteins 0.000 claims description 12
- 238000010804 cDNA synthesis Methods 0.000 claims description 12
- 239000002299 complementary DNA Substances 0.000 claims description 12
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 10
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical group CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 9
- 229930024421 Adenine Natural products 0.000 claims description 5
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 claims description 5
- 229960000643 adenine Drugs 0.000 claims description 5
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 5
- 210000004698 lymphocyte Anatomy 0.000 claims description 5
- 229940113082 thymine Drugs 0.000 claims description 5
- 108091028664 Ribonucleotide Proteins 0.000 claims description 4
- 210000003719 b-lymphocyte Anatomy 0.000 claims description 4
- 230000002934 lysing effect Effects 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 238000010839 reverse transcription Methods 0.000 claims description 4
- 239000002336 ribonucleotide Substances 0.000 claims description 4
- 125000002652 ribonucleotide group Chemical group 0.000 claims description 4
- 238000011222 transcriptome analysis Methods 0.000 claims description 4
- 238000000137 annealing Methods 0.000 claims description 3
- 230000020411 cell activation Effects 0.000 claims description 3
- 230000003915 cell function Effects 0.000 claims description 3
- 230000002062 proliferating effect Effects 0.000 claims description 3
- 108091026890 Coding region Proteins 0.000 claims description 2
- 239000003155 DNA primer Substances 0.000 claims description 2
- 150000001413 amino acids Chemical class 0.000 claims description 2
- 230000001413 cellular effect Effects 0.000 claims description 2
- 230000009977 dual effect Effects 0.000 claims description 2
- 239000000839 emulsion Substances 0.000 claims description 2
- 238000009396 hybridization Methods 0.000 claims description 2
- 239000003094 microcapsule Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 229920001184 polypeptide Polymers 0.000 claims description 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 2
- 102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 239000000523 sample Substances 0.000 description 13
- 210000003071 memory t lymphocyte Anatomy 0.000 description 6
- 102100033215 DNA nucleotidylexotransferase Human genes 0.000 description 4
- 210000002865 immune cell Anatomy 0.000 description 4
- 210000000265 leukocyte Anatomy 0.000 description 4
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 4
- 239000011324 bead Substances 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 239000012636 effector Substances 0.000 description 2
- 210000003162 effector t lymphocyte Anatomy 0.000 description 2
- 239000008240 homogeneous mixture Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 210000003289 regulatory T cell Anatomy 0.000 description 2
- 241000271566 Aves Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 206010017533 Fungal infection Diseases 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 210000000612 antigen-presenting cell Anatomy 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- -1 droplet Substances 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 210000001806 memory b lymphocyte Anatomy 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 208000025113 myeloid leukemia Diseases 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762511949P | 2017-05-26 | 2017-05-26 | |
| US62/511,949 | 2017-05-26 | ||
| PCT/US2018/034768 WO2018218222A1 (en) | 2017-05-26 | 2018-05-25 | High-throughput polynucleotide library sequencing and transcriptome analysis |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020523034A JP2020523034A (ja) | 2020-08-06 |
| JP2020523034A5 true JP2020523034A5 (enExample) | 2021-07-26 |
| JP7308187B2 JP7308187B2 (ja) | 2023-07-13 |
Family
ID=62621068
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020515835A Active JP7308187B2 (ja) | 2017-05-26 | 2018-05-25 | ハイスループットポリヌクレオチドライブラリーシーケンシングおよびトランスクリプトーム解析法 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US12049667B2 (enExample) |
| EP (1) | EP3631012B1 (enExample) |
| JP (1) | JP7308187B2 (enExample) |
| KR (1) | KR102550778B1 (enExample) |
| CN (1) | CN111148849A (enExample) |
| AU (1) | AU2018273999A1 (enExample) |
| CA (1) | CA3064205A1 (enExample) |
| ES (1) | ES2926722T3 (enExample) |
| MA (1) | MA49352A (enExample) |
| WO (1) | WO2018218222A1 (enExample) |
Families Citing this family (55)
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| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| CA2865575C (en) | 2012-02-27 | 2024-01-16 | Cellular Research, Inc. | Compositions and kits for molecular counting |
| ES2857908T3 (es) | 2013-08-28 | 2021-09-29 | Becton Dickinson Co | Análisis masivamente paralelo de células individuales |
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| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| KR102363716B1 (ko) | 2016-09-26 | 2022-02-18 | 셀룰러 리서치, 인크. | 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정 |
| US11319583B2 (en) | 2017-02-01 | 2022-05-03 | Becton, Dickinson And Company | Selective amplification using blocking oligonucleotides |
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| WO2018226293A1 (en) | 2017-06-05 | 2018-12-13 | Becton, Dickinson And Company | Sample indexing for single cells |
| WO2019213294A1 (en) | 2018-05-03 | 2019-11-07 | Becton, Dickinson And Company | High throughput multiomics sample analysis |
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| EP3894552B1 (en) | 2018-12-13 | 2026-01-28 | Becton, Dickinson and Company | Selective extension in single cell whole transcriptome analysis |
| JP2022515466A (ja) * | 2018-12-28 | 2022-02-18 | ナショナル ユニヴァーシティー オブ シンガポール | 標的化された相補的ｄｎａ富化のための方法 |
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| CN114051534B (zh) | 2019-07-22 | 2025-02-21 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
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| EP4041295A4 (en) * | 2019-10-09 | 2024-04-10 | Edward Fritsch | MULTI-DOMAIN PROTEIN VACCINE |
| CN114729350A (zh) * | 2019-11-08 | 2022-07-08 | 贝克顿迪金森公司 | 使用随机引发获得用于免疫组库测序的全长v(d)j信息 |
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- 2018-05-25 AU AU2018273999A patent/AU2018273999A1/en not_active Abandoned
- 2018-05-25 CA CA3064205A patent/CA3064205A1/en active Pending
- 2018-05-25 MA MA049352A patent/MA49352A/fr unknown
- 2018-05-25 KR KR1020197038441A patent/KR102550778B1/ko active Active
- 2018-05-25 US US16/616,424 patent/US12049667B2/en active Active
- 2018-05-25 CN CN201880049800.9A patent/CN111148849A/zh active Pending
- 2018-05-25 WO PCT/US2018/034768 patent/WO2018218222A1/en not_active Ceased
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