JP2018509896A - 高処理能力研究用の単一細胞核酸 - Google Patents

高処理能力研究用の単一細胞核酸 Download PDF

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JP2018509896A
JP2018509896A JP2017542907A JP2017542907A JP2018509896A JP 2018509896 A JP2018509896 A JP 2018509896A JP 2017542907 A JP2017542907 A JP 2017542907A JP 2017542907 A JP2017542907 A JP 2017542907A JP 2018509896 A JP2018509896 A JP 2018509896A
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linker
barcode
primer
sequence
capture
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JP2018509896A5 (enExample
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ジー コナント キャロリン
ジー コナント キャロリン
ホウェ チャーン ズー
ホウェ チャーン ズー
エイ エイ ウェスト ジェイソン
エイ エイ ウェスト ジェイソン
ワーン シャオフィ
ワーン シャオフィ
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フリューダイム・コーポレイション
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Fluid Mechanics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2017542907A 2015-02-27 2016-02-26 高処理能力研究用の単一細胞核酸 Pending JP2018509896A (ja)

Applications Claiming Priority (3)

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US201562126349P 2015-02-27 2015-02-27
US62/126,349 2015-02-27
PCT/US2016/019952 WO2016138490A1 (en) 2015-02-27 2016-02-26 Single-cell nucleic acids for high-throughput studies

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JP2018509896A true JP2018509896A (ja) 2018-04-12
JP2018509896A5 JP2018509896A5 (enExample) 2019-04-04

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US (2) US10190163B2 (enExample)
EP (1) EP3262214B1 (enExample)
JP (1) JP2018509896A (enExample)
CN (1) CN107250445A (enExample)
CA (1) CA2976681A1 (enExample)
SG (1) SG11201706636PA (enExample)
WO (1) WO2016138490A1 (enExample)

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JP2022511398A (ja) * 2018-10-01 2022-01-31 ベクトン・ディキンソン・アンド・カンパニー ５’転写物配列の決定
JP2022551551A (ja) * 2019-10-11 2022-12-12 ブラックホーク ジェノミクス エルエルシー ロングリードシークエンシングを用いたゲノム構造バリアントの同定
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US11993814B2 (en) 2009-12-15 2024-05-28 Becton, Dickinson And Company Digital counting of individual molecules by stochastic attachment of diverse labels
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
US12084712B2 (en) 2017-06-05 2024-09-10 Becton, Dickinson And Company Sample indexing for single cells
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US12188010B2 (en) 2020-01-29 2025-01-07 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US12331351B2 (en) 2016-05-31 2025-06-17 Becton, Dickinson And Company Error correction in amplification of samples
US12378594B2 (en) 2020-05-14 2025-08-05 Becton, Dickinson And Company Primers for immune repertoire profiling
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
US12421548B2 (en) 2018-05-03 2025-09-23 Becton, Dickinson And Company High throughput multiomics sample analysis
US12606859B2 (en) 2020-01-13 2026-04-21 Becton, Dickinson And Company Cell capture using DU-containing oligonucleotides
US12630874B1 (en) 2019-11-08 2026-05-19 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing

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CN110312825A (zh) 2016-10-24 2019-10-08 吉内恩福赛克公司 隐藏核酸内存在的信息
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JP2020528740A (ja) * 2017-06-20 2020-10-01 プソマーゲン， インコーポレイテッドＰｓｏｍａｇｅｎ， Ｉｎｃ． 固有分子識別子を用いてライブラリーを調製するための方法及びシステム
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JP7413408B2 (ja) 2019-05-07 2024-01-15 バイオ－ラッド ラボラトリーズ インコーポレイテッド 自動化された単一細胞処理のためのシステムおよび方法
US11273439B2 (en) 2019-05-07 2022-03-15 Bio-Rad Laboratories, Inc. System and method for target material retrieval from microwells
CN118291246A (zh) 2019-06-14 2024-07-05 伯乐实验室有限公司 用于自动化单细胞处理和分析的系统和方法
JP2022544101A (ja) * 2019-08-08 2022-10-17 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル 表現型の規定されたｂ細胞及びｔ細胞のサブセットにおける、ｂ細胞とｔ細胞のトランスクリプトームの分析のためのｒｎａシークエンス法
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JP2023547732A (ja) * 2020-11-03 2023-11-13 エイシーティ・ジェノミックス（アイピー）リミテッド 遺伝子改変を検出するための標的化シークエンシング方法およびそのキット
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Cited By (19)

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Publication number Priority date Publication date Assignee Title
US12060607B2 (en) 2009-12-15 2024-08-13 Becton, Dickinson And Company Digital counting of individual molecules by stochastic attachment of diverse labels
US12571038B2 (en) 2009-12-15 2026-03-10 Becton, Dickinson And Company Digital counting of individual molecules by stochastic attachment of diverse labels
US11993814B2 (en) 2009-12-15 2024-05-28 Becton, Dickinson And Company Digital counting of individual molecules by stochastic attachment of diverse labels
US12331351B2 (en) 2016-05-31 2025-06-17 Becton, Dickinson And Company Error correction in amplification of samples
US12371729B2 (en) 2017-06-05 2025-07-29 Becton, Dickinson And Company Sample indexing for single cells
US12084712B2 (en) 2017-06-05 2024-09-10 Becton, Dickinson And Company Sample indexing for single cells
US12421548B2 (en) 2018-05-03 2025-09-23 Becton, Dickinson And Company High throughput multiomics sample analysis
JP7670607B2 (ja) 2018-10-01 2025-04-30 ベクトン・ディキンソン・アンド・カンパニー ５’転写物配列の決定
JP2022511398A (ja) * 2018-10-01 2022-01-31 ベクトン・ディキンソン・アンド・カンパニー ５’転写物配列の決定
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
JP2022551551A (ja) * 2019-10-11 2022-12-12 ブラックホーク ジェノミクス エルエルシー ロングリードシークエンシングを用いたゲノム構造バリアントの同定
US12630874B1 (en) 2019-11-08 2026-05-19 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
US12606859B2 (en) 2020-01-13 2026-04-21 Becton, Dickinson And Company Cell capture using DU-containing oligonucleotides
US12188010B2 (en) 2020-01-29 2025-01-07 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US12378594B2 (en) 2020-05-14 2025-08-05 Becton, Dickinson And Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis

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US10954560B2 (en) 2021-03-23
EP3262214B1 (en) 2023-10-25
US10190163B2 (en) 2019-01-29
WO2016138490A1 (en) 2016-09-01
SG11201706636PA (en) 2017-09-28
CN107250445A (zh) 2017-10-13
CA2976681A1 (en) 2016-09-01
US20190185929A1 (en) 2019-06-20
EP3262214A4 (en) 2018-07-25
EP3262214A1 (en) 2018-01-03
US20160251714A1 (en) 2016-09-01

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