JP2015512259A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2015512259A5 JP2015512259A5 JP2015503300A JP2015503300A JP2015512259A5 JP 2015512259 A5 JP2015512259 A5 JP 2015512259A5 JP 2015503300 A JP2015503300 A JP 2015503300A JP 2015503300 A JP2015503300 A JP 2015503300A JP 2015512259 A5 JP2015512259 A5 JP 2015512259A5
- Authority
- JP
- Japan
- Prior art keywords
- recombinant protein
- maintained
- homogenization
- producing
- prokaryotic host
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims description 18
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims description 18
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 238000013019 agitation Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000003556 assay Methods 0.000 claims description 3
- 102000039446 nucleic acids Human genes 0.000 claims description 3
- 108020004707 nucleic acids Proteins 0.000 claims description 3
- 150000007523 nucleic acids Chemical class 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 238000000034 method Methods 0.000 claims 20
- VOJUXHHACRXLTD-UHFFFAOYSA-N 1,4-dihydroxy-2-naphthoic acid Chemical compound C1=CC=CC2=C(O)C(C(=O)O)=CC(O)=C21 VOJUXHHACRXLTD-UHFFFAOYSA-N 0.000 claims 4
- 238000000265 homogenisation Methods 0.000 claims 4
- 238000004255 ion exchange chromatography Methods 0.000 claims 4
- VOJUXHHACRXLTD-UHFFFAOYSA-M 1,4-dihydroxy-2-naphthoate Chemical compound C1=CC=C2C(O)=CC(C([O-])=O)=C(O)C2=C1 VOJUXHHACRXLTD-UHFFFAOYSA-M 0.000 claims 2
- 108010065780 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase Proteins 0.000 claims 2
- 108010089355 dihydroneopterin aldolase Proteins 0.000 claims 2
- 238000001914 filtration Methods 0.000 claims 2
- 238000004128 high performance liquid chromatography Methods 0.000 claims 2
- 241000589151 Azotobacter Species 0.000 claims 1
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 241000588914 Enterobacter Species 0.000 claims 1
- 241000588698 Erwinia Species 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 claims 1
- 241000588748 Klebsiella Species 0.000 claims 1
- 238000005481 NMR spectroscopy Methods 0.000 claims 1
- 241001057811 Paracoccus <mealybug> Species 0.000 claims 1
- 241000588769 Proteus <enterobacteria> Species 0.000 claims 1
- 241000589516 Pseudomonas Species 0.000 claims 1
- 241000589180 Rhizobium Species 0.000 claims 1
- 241000607142 Salmonella Species 0.000 claims 1
- 241000607720 Serratia Species 0.000 claims 1
- 241000607768 Shigella Species 0.000 claims 1
- 238000012801 analytical assay Methods 0.000 claims 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims 1
- 238000000855 fermentation Methods 0.000 claims 1
- 230000004151 fermentation Effects 0.000 claims 1
- 238000004949 mass spectrometry Methods 0.000 claims 1
- 101150087021 menE gene Proteins 0.000 claims 1
- 229910052760 oxygen Inorganic materials 0.000 claims 1
- 239000001301 oxygen Substances 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 claims 1
- 239000003755 preservative agent Substances 0.000 claims 1
- 230000002335 preservative effect Effects 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 238000004007 reversed phase HPLC Methods 0.000 claims 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261616297P | 2012-03-27 | 2012-03-27 | |
| US61/616,297 | 2012-03-27 | ||
| PCT/US2013/031383 WO2013148249A1 (en) | 2012-03-27 | 2013-03-14 | Improved harvest operations for recombinant proteins |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017195016A Division JP2018046825A (ja) | 2012-03-27 | 2017-10-05 | 組換えタンパク質の改良された収集操作 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015512259A JP2015512259A (ja) | 2015-04-27 |
| JP2015512259A5 true JP2015512259A5 (enExample) | 2016-05-12 |
| JP6225162B2 JP6225162B2 (ja) | 2017-11-01 |
Family
ID=48040433
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015503300A Active JP6225162B2 (ja) | 2012-03-27 | 2013-03-14 | 組換えタンパク質の改良された収集操作 |
| JP2017195016A Pending JP2018046825A (ja) | 2012-03-27 | 2017-10-05 | 組換えタンパク質の改良された収集操作 |
| JP2019049717A Withdrawn JP2019141049A (ja) | 2012-03-27 | 2019-03-18 | 組換えタンパク質の改良された収集操作 |
| JP2020137053A Withdrawn JP2020202849A (ja) | 2012-03-27 | 2020-08-14 | 組換えタンパク質の改良された収集操作 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017195016A Pending JP2018046825A (ja) | 2012-03-27 | 2017-10-05 | 組換えタンパク質の改良された収集操作 |
| JP2019049717A Withdrawn JP2019141049A (ja) | 2012-03-27 | 2019-03-18 | 組換えタンパク質の改良された収集操作 |
| JP2020137053A Withdrawn JP2020202849A (ja) | 2012-03-27 | 2020-08-14 | 組換えタンパク質の改良された収集操作 |
Country Status (20)
| Country | Link |
|---|---|
| US (9) | US20140080180A1 (enExample) |
| EP (1) | EP2831261B1 (enExample) |
| JP (4) | JP6225162B2 (enExample) |
| KR (2) | KR20200037425A (enExample) |
| CN (2) | CN109880867A (enExample) |
| AR (2) | AR090340A1 (enExample) |
| AU (3) | AU2013240280A1 (enExample) |
| BR (1) | BR112014023176B1 (enExample) |
| CA (2) | CA3086730A1 (enExample) |
| ES (1) | ES2654424T3 (enExample) |
| HR (1) | HRP20171966T1 (enExample) |
| IL (1) | IL234377B (enExample) |
| MX (3) | MX365947B (enExample) |
| MY (1) | MY171729A (enExample) |
| PL (1) | PL2831261T3 (enExample) |
| RU (2) | RU2017139267A (enExample) |
| SG (4) | SG10202007206WA (enExample) |
| SI (1) | SI2831261T1 (enExample) |
| WO (1) | WO2013148249A1 (enExample) |
| ZA (1) | ZA201406434B (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6201037B1 (en) | 1986-01-28 | 2001-03-13 | Ophthalmic Research Group International, Inc. | Plastic lens composition and method for the production thereof |
| EP3469091A1 (en) | 2016-06-10 | 2019-04-17 | Lonza Ltd | Method for stabilizing proteins |
| SG11201903605TA (en) * | 2016-11-07 | 2019-05-30 | Seattle Genetics Inc | Distribution of engineered-cysteine caps |
| KR102825164B1 (ko) * | 2018-03-27 | 2025-06-26 | 브리스톨-마이어스 스큅 컴퍼니 | 자외선 신호를 사용한 단백질 농도의 실시간 모니터링 |
| PL3857237T3 (pl) * | 2019-01-16 | 2023-07-10 | Regeneron Pharmaceuticals, Inc. | Sposoby charakteryzowania mostka disiarczkowego |
| CN109725088B (zh) * | 2019-03-16 | 2021-05-18 | 丁立平 | 一种测定豆芽中2-萘甲酸及其衍生物的气相色谱-质谱联用法 |
| CN110305903B (zh) * | 2019-07-31 | 2021-10-01 | 江苏璟泽生物医药有限公司 | 重组人促卵泡激素及其制备方法 |
| CN113155776A (zh) * | 2021-04-29 | 2021-07-23 | 华东交通大学 | 一种柑桔最佳采收期预测方法 |
| FR3146146A1 (fr) | 2023-02-24 | 2024-08-30 | Bgene Genetics | Production d’acide 1-4-dihydroxy-2-naphtoïque par Escherichia coli |
Family Cites Families (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2036891B (en) | 1978-12-05 | 1983-05-05 | Windsor Smith C | Change speed gear |
| US4515893A (en) | 1979-04-26 | 1985-05-07 | Ortho Pharmaceutical Corporation | Hybrid cell line for producing complement-fixing monoclonal antibody to human T cells |
| ZA811368B (en) | 1980-03-24 | 1982-04-28 | Genentech Inc | Bacterial polypedtide expression employing tryptophan promoter-operator |
| GB8308235D0 (en) | 1983-03-25 | 1983-05-05 | Celltech Ltd | Polypeptides |
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| US5672347A (en) | 1984-07-05 | 1997-09-30 | Genentech, Inc. | Tumor necrosis factor antagonists and their use |
| JPS6147500A (ja) | 1984-08-15 | 1986-03-07 | Res Dev Corp Of Japan | キメラモノクロ−ナル抗体及びその製造法 |
| EP0173494A3 (en) | 1984-08-27 | 1987-11-25 | The Board Of Trustees Of The Leland Stanford Junior University | Chimeric receptors by dna splicing and expression |
| GB8422238D0 (en) | 1984-09-03 | 1984-10-10 | Neuberger M S | Chimeric proteins |
| JPS61134325A (ja) | 1984-12-04 | 1986-06-21 | Teijin Ltd | ハイブリツド抗体遺伝子の発現方法 |
| EP0247091B1 (en) | 1985-11-01 | 1993-09-29 | Xoma Corporation | Modular assembly of antibody genes, antibodies prepared thereby and use |
| US5225539A (en) | 1986-03-27 | 1993-07-06 | Medical Research Council | Recombinant altered antibodies and methods of making altered antibodies |
| US4946778A (en) | 1987-09-21 | 1990-08-07 | Genex Corporation | Single polypeptide chain binding molecules |
| IL85035A0 (en) | 1987-01-08 | 1988-06-30 | Int Genetic Eng | Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same |
| US5091313A (en) | 1988-08-05 | 1992-02-25 | Tanox Biosystems, Inc. | Antigenic epitopes of IgE present on B cell but not basophil surface |
| US5720937A (en) | 1988-01-12 | 1998-02-24 | Genentech, Inc. | In vivo tumor detection assay |
| WO1989012463A1 (en) | 1988-06-21 | 1989-12-28 | Genentech, Inc. | Method and therapeutic compositions for the treatment of myocardial infarction |
| US5530101A (en) | 1988-12-28 | 1996-06-25 | Protein Design Labs, Inc. | Humanized immunoglobulins |
| DK0602126T3 (da) | 1991-08-14 | 2003-06-16 | Genentech Inc | Immunoglobulinvarianter til specifikke FC epsilon-receptorer |
| WO1993008829A1 (en) | 1991-11-04 | 1993-05-13 | The Regents Of The University Of California | Compositions that mediate killing of hiv-infected cells |
| US5288931A (en) | 1991-12-06 | 1994-02-22 | Genentech, Inc. | Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation |
| EP0656064B1 (en) | 1992-08-17 | 1997-03-05 | Genentech, Inc. | Bispecific immunoadhesins |
| AU687755B2 (en) | 1992-08-21 | 1998-03-05 | Genentech Inc. | Method for treating an LFA-1-mediated disorder |
| US5736137A (en) | 1992-11-13 | 1998-04-07 | Idec Pharmaceuticals Corporation | Therapeutic application of chimeric and radiolabeled antibodies to human B lymphocyte restricted differentiation antigen for treatment of B cell lymphoma |
| EP0733207B1 (en) | 1993-12-10 | 1997-08-27 | Genentech, Inc. | Methods for diagnosis of allergy and screening of anti-allergy therapeutics |
| JP3825798B2 (ja) | 1994-01-18 | 2006-09-27 | ジェネンテク，インコーポレイテッド | ＩｇＥアンタゴニストを用いる寄生虫感染症の治療法 |
| US5702946A (en) | 1994-03-03 | 1997-12-30 | Genentech, Inc. | Anti-IL-8 monoclonal antibodies for treatment of inflammatory disorders |
| US5639635A (en) | 1994-11-03 | 1997-06-17 | Genentech, Inc. | Process for bacterial production of polypeptides |
| IL117645A (en) | 1995-03-30 | 2005-08-31 | Genentech Inc | Vascular endothelial cell growth factor antagonists for use as medicaments in the treatment of age-related macular degeneration |
| JPH11507535A (ja) | 1995-06-07 | 1999-07-06 | イムクローン システムズ インコーポレイテッド | 腫瘍の成長を抑制する抗体および抗体フラグメント類 |
| ES2183131T3 (es) | 1996-01-23 | 2003-03-16 | Genentech Inc | Anticuerpos anti-cd18 utilizados contra el ictus cerebral. |
| US7147851B1 (en) | 1996-08-15 | 2006-12-12 | Millennium Pharmaceuticals, Inc. | Humanized immunoglobulin reactive with α4β7 integrin |
| DK0941344T3 (da) | 1996-11-27 | 2004-09-27 | Genentech Inc | Humaniserede anti-CD11a-antistoffer |
| IL132240A0 (en) | 1997-04-07 | 2001-03-19 | Genentech Inc | Anti-vegf antibodies |
| EP1860187B1 (en) | 1997-05-15 | 2011-07-13 | Genentech, Inc. | Apo-2 receptor |
| US6946129B1 (en) | 1999-06-08 | 2005-09-20 | Seattle Genetics, Inc. | Recombinant anti-CD40 antibody and uses thereof |
| BR0015224A (pt) | 1999-10-29 | 2002-07-09 | Genentech Inc | Anticorpo anti-antìgeno de célula estaminal prostática (psca) isolado, anticorpo monoclonal anti-psca, ácido nucleico isolado, vetor de expressão, célula, célula hospedeira, método de produção de anticorpo, composição método in vitro para matar uma célula cancerosa que expresse psca, uso de uma anticorpo monoclonal anti-psca e artigo industrializado |
| US6946271B2 (en) * | 2000-09-20 | 2005-09-20 | Degussa Ag | Nucleotide sequences which code for the menE gene |
| US6979556B2 (en) | 2000-12-14 | 2005-12-27 | Genentech, Inc. | Separate-cistron contructs for secretion of aglycosylated antibodies from prokaryotes |
| EP1944320A1 (en) | 2002-12-16 | 2008-07-16 | Genentech, Inc. | Immunoglobulin variants and uses thereof |
| NZ543200A (en) * | 2003-03-26 | 2009-01-31 | Meiji Dairies Corp | Method for stabilizing 1,4-dihydroxy-2-naphthoic acid |
| ATE353969T1 (de) * | 2003-08-13 | 2007-03-15 | Sandoz Ag | Expressionsvektoren, transformierte wirtszellen und fermentationsverfahren zur herstellung rekombinanter polypeptide |
| JP4972412B2 (ja) * | 2004-02-04 | 2012-07-11 | メルク・シャープ・エンド・ドーム・コーポレイション | Ｅ．コリ発酵によるプラスミドｄｎａの大規模生産のための方法 |
| US7361764B2 (en) * | 2004-07-27 | 2008-04-22 | Sgx Pharmaceuticals, Inc. | Pyrrolo-pyridine kinase modulators |
| CN101115832A (zh) * | 2004-11-26 | 2008-01-30 | 协和发酵工业株式会社 | 工业上有用的微生物 |
| MX2008014150A (es) * | 2006-05-05 | 2009-02-04 | Gangagen Inc | Actividades antimicrobianas derivadas de fago. |
| JP5305615B2 (ja) * | 2006-06-05 | 2013-10-02 | 株式会社Ａｄｅｋａ | メナキノンの新規生合成経路に関与するタンパク質の遺伝子群を利用したメナキノンの製造方法 |
| SG162834A1 (en) * | 2006-07-14 | 2010-07-29 | Genentech Inc | Refolding of recombinant proteins |
| HUE037162T2 (hu) * | 2007-07-09 | 2018-08-28 | Genentech Inc | Diszulfidkötés redukciójának megelõzése polipeptidek rekombináns elõállítása során |
| CN101665798B (zh) * | 2008-09-06 | 2012-11-21 | 浙江我武生物科技股份有限公司 | 一种制备重组人血清白蛋白与干扰素α融合蛋白的方法 |
| CN101503470A (zh) * | 2008-09-27 | 2009-08-12 | 上海交通大学 | rhDSL重组蛋白及其制备方法 |
| JP2010088387A (ja) * | 2008-10-10 | 2010-04-22 | Waseda Univ | 放線菌内で目的タンパク質を誘導発現可能なプロモーター |
-
2013
- 2013-03-14 RU RU2017139267A patent/RU2017139267A/ru not_active Application Discontinuation
- 2013-03-14 AR ARP130100828A patent/AR090340A1/es active IP Right Grant
- 2013-03-14 SG SG10202007206WA patent/SG10202007206WA/en unknown
- 2013-03-14 SI SI201330894T patent/SI2831261T1/en unknown
- 2013-03-14 MY MYPI2014002775A patent/MY171729A/en unknown
- 2013-03-14 WO PCT/US2013/031383 patent/WO2013148249A1/en not_active Ceased
- 2013-03-14 US US13/826,166 patent/US20140080180A1/en not_active Abandoned
- 2013-03-14 CA CA3086730A patent/CA3086730A1/en not_active Abandoned
- 2013-03-14 EP EP13713295.7A patent/EP2831261B1/en active Active
- 2013-03-14 ES ES13713295.7T patent/ES2654424T3/es active Active
- 2013-03-14 CA CA2866753A patent/CA2866753C/en active Active
- 2013-03-14 BR BR112014023176-1A patent/BR112014023176B1/pt active IP Right Grant
- 2013-03-14 KR KR1020207008828A patent/KR20200037425A/ko not_active Ceased
- 2013-03-14 KR KR1020147026772A patent/KR102096678B1/ko active Active
- 2013-03-14 JP JP2015503300A patent/JP6225162B2/ja active Active
- 2013-03-14 HR HRP20171966TT patent/HRP20171966T1/hr unknown
- 2013-03-14 MX MX2014011558A patent/MX365947B/es active IP Right Grant
- 2013-03-14 RU RU2014137554A patent/RU2636353C2/ru active
- 2013-03-14 AU AU2013240280A patent/AU2013240280A1/en not_active Abandoned
- 2013-03-14 CN CN201910197577.9A patent/CN109880867A/zh active Pending
- 2013-03-14 SG SG10201508420VA patent/SG10201508420VA/en unknown
- 2013-03-14 CN CN201380027623.1A patent/CN104350156B/zh active Active
- 2013-03-14 SG SG11201406112TA patent/SG11201406112TA/en unknown
- 2013-03-14 SG SG10201912877SA patent/SG10201912877SA/en unknown
- 2013-03-14 PL PL13713295T patent/PL2831261T3/pl unknown
-
2014
- 2014-08-31 IL IL234377A patent/IL234377B/en active IP Right Grant
- 2014-09-02 ZA ZA2014/06434A patent/ZA201406434B/en unknown
- 2014-09-25 MX MX2018007677A patent/MX2018007677A/es unknown
- 2014-09-25 MX MX2019007032A patent/MX2019007032A/es unknown
- 2014-09-26 US US14/497,964 patent/US9765379B2/en active Active
-
2015
- 2015-06-09 US US14/734,848 patent/US20160130624A1/en not_active Abandoned
-
2017
- 2017-04-13 AU AU2017202484A patent/AU2017202484B2/en active Active
- 2017-10-05 JP JP2017195016A patent/JP2018046825A/ja active Pending
-
2018
- 2018-02-28 US US15/908,526 patent/US20180291411A1/en not_active Abandoned
-
2019
- 2019-02-07 US US16/270,494 patent/US20200199639A1/en not_active Abandoned
- 2019-03-18 JP JP2019049717A patent/JP2019141049A/ja not_active Withdrawn
- 2019-09-05 AU AU2019226192A patent/AU2019226192A1/en not_active Abandoned
- 2019-10-09 AR ARP190102873A patent/AR116615A2/es unknown
-
2020
- 2020-08-14 JP JP2020137053A patent/JP2020202849A/ja not_active Withdrawn
- 2020-10-09 US US17/067,059 patent/US20210171997A1/en not_active Abandoned
-
2022
- 2022-07-13 US US17/864,255 patent/US20230069966A1/en not_active Abandoned
-
2024
- 2024-01-22 US US18/419,368 patent/US20240158825A1/en not_active Abandoned
- 2024-12-20 US US18/990,600 patent/US20250137027A1/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Cocolin et al. | Culture independent methods to assess the diversity and dynamics of microbiota during food fermentation | |
| Yang et al. | Highly efficient production of soluble proteins from insoluble inclusion bodies by a two-step-denaturing and refolding method | |
| An et al. | Expression of a functional recombinant human basic fibroblast growth factor from transgenic rice seeds | |
| BR112015022971A2 (pt) | métodos de fabricação para controlar o teor de lisina c-terminal, galactose e ácido siálico em proteínas recombinantes | |
| JP2017513479A5 (enExample) | ||
| SI2831261T1 (en) | A process for the production of low-level recombinant proteins DHNA (1,4-dihydroxy-2-naphthoate) | |
| Readi et al. | On the isolation of single basic amino acids with electrodialysis for the production of biobased chemicals | |
| Olvera-Rosales et al. | ACE-inhibitory activity of whey proteins fractions derived of fermentation by Lacticaseibacillus rhamnosus GG and Streptococcus thermophilus SY-102 | |
| Hui et al. | Biosynthesis of putrescine from L-arginine using engineered Escherichia coli whole cells | |
| MY187130A (en) | Variants of gal2 transporter and their uses | |
| Levante et al. | Expression of DinJ-YafQ System of Lactobacillus casei group strains in response to food processing stresses | |
| Borges et al. | Bioactive peptides from milk proteins with antioxidant, anti-inflammatory, and antihypertensive activities | |
| Huang et al. | Gene expression and epigenetic modification of aromatase during sex reversal and gonadal development in blotched snakehead (Channa maculata) | |
| Bonnaud et al. | Haloarchaea as Promising Chassis to Green Chemistry | |
| Sitzia et al. | Unveiling IL-33/ST2 pathway unbalance in cardiac remodeling due to obesity in Zucker fatty rats | |
| CN102586202A (zh) | 一种硫氧还蛋白及其制备和应用 | |
| CN106928315A (zh) | 基于氧化石墨烯的核酸结合蛋白提取方法 | |
| CN103059123B (zh) | 一种重组猪干扰素β1及其编码基因和表达方法 | |
| CN109777807B (zh) | 一种在线粒体中定位表达外源蛋白的方法 | |
| CN103266105B (zh) | 通过翻译暂停序列理性重设计改善蛋白质可溶性表达的方法 | |
| He et al. | Genomic, probiotic, and functional properties of bacteroides dorei RX2020 isolated from gut microbiota | |
| JP6499510B2 (ja) | 遺伝子組み換え酵母及びそれを用いた５−アミノレブリン酸の製造法 | |
| Polumahanthi et al. | Tissue culture, molecular and genetic approaches to sorghum crop improvement–A review | |
| Li et al. | Cold Shock Proteins Mediate Transcription of Ribosomal RNA in Escherichia coli Under Cold-Stress Conditions | |
| Chen et al. | Functional analysis of the HbREF1 promoter from Hevea brasiliensis and its response to phytohormones |