AR090340A1 - Operaciones de cosecha mejoradas para proteinas recombinadas - Google Patents

Operaciones de cosecha mejoradas para proteinas recombinadas

Info

Publication number
AR090340A1
AR090340A1 ARP130100828A ARP130100828A AR090340A1 AR 090340 A1 AR090340 A1 AR 090340A1 AR P130100828 A ARP130100828 A AR P130100828A AR P130100828 A ARP130100828 A AR P130100828A AR 090340 A1 AR090340 A1 AR 090340A1
Authority
AR
Argentina
Prior art keywords
recombinant protein
host cell
prokaryotic host
filtered volume
dhna
Prior art date
Application number
ARP130100828A
Other languages
English (en)
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of AR090340A1 publication Critical patent/AR090340A1/es

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/42Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Métodos para la producción de una proteína recombinante que comprende (a) la fermentación de una célula huésped procariota, donde dicha célula huésped procariota ha sido transformada con un ácido nucleico que codifica dicha proteína recombinante, la cosecha de dicha proteína recombinante en condiciones donde los niveles de dO₂ son superiores a 0%, la purificación de dicha proteína recombinante hasta un volumen filtrado, donde dicho volumen filtrado no contiene una cantidad detectable de aducto de DHNA-proteína recombinante, medida por un ensayo de IEC a 310 nm. Además un método para la producción de una proteína recombinante que comprende la fermentación de una célula huésped procariota con deleción del gen menE, donde dicha célula huésped procariota ha sido transformada con un ácido nucleico que codifica dicha proteína recombinante, la cosecha de dicha proteína recombinante; y la purificación de dicha proteína recombinante hasta un volumen filtrado, donde dicho volumen filtrado no contiene una cantidad detectable de aducto de DHNA-proteína recombinante, medida por un ensayo de IEC a 310 nm, y donde el rendimiento de proteína recombinante se incrementa alrededor de 20% o más.
ARP130100828A 2012-03-27 2013-03-14 Operaciones de cosecha mejoradas para proteinas recombinadas AR090340A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261616297P 2012-03-27 2012-03-27

Publications (1)

Publication Number Publication Date
AR090340A1 true AR090340A1 (es) 2014-11-05

Family

ID=48040433

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP130100828A AR090340A1 (es) 2012-03-27 2013-03-14 Operaciones de cosecha mejoradas para proteinas recombinadas
ARP190102873A AR116615A2 (es) 2012-03-27 2019-10-09 Operaciones de cosecha mejoradas para proteínas recombinadas

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP190102873A AR116615A2 (es) 2012-03-27 2019-10-09 Operaciones de cosecha mejoradas para proteínas recombinadas

Country Status (21)

Country Link
US (8) US20140080180A1 (es)
EP (1) EP2831261B1 (es)
JP (4) JP6225162B2 (es)
KR (2) KR102096678B1 (es)
CN (2) CN104350156B (es)
AR (2) AR090340A1 (es)
AU (3) AU2013240280A1 (es)
BR (1) BR112014023176B1 (es)
CA (2) CA3086730A1 (es)
ES (1) ES2654424T3 (es)
HK (1) HK1207120A1 (es)
HR (1) HRP20171966T1 (es)
IL (1) IL234377B (es)
MX (3) MX365947B (es)
MY (1) MY171729A (es)
PL (1) PL2831261T3 (es)
RU (2) RU2636353C2 (es)
SG (4) SG10201912877SA (es)
SI (1) SI2831261T1 (es)
WO (1) WO2013148249A1 (es)
ZA (1) ZA201406434B (es)

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US20210024873A1 (en) * 2018-03-27 2021-01-28 Bristol-Myers Squibb Company Real-time monitoring of protein concentration using ultraviolet signal
WO2020150491A1 (en) 2019-01-16 2020-07-23 Regeneron Pharmaceuticals, Inc. Methods for characterizing disulfide bonds
CN109725088B (zh) * 2019-03-16 2021-05-18 丁立平 一种测定豆芽中2-萘甲酸及其衍生物的气相色谱-质谱联用法
CN110305903B (zh) * 2019-07-31 2021-10-01 江苏璟泽生物医药有限公司 重组人促卵泡激素及其制备方法
CN113155776A (zh) * 2021-04-29 2021-07-23 华东交通大学 一种柑桔最佳采收期预测方法
FR3146146A1 (fr) 2023-02-24 2024-08-30 Bgene Genetics Production d’acide 1-4-dihydroxy-2-naphtoïque par Escherichia coli

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Also Published As

Publication number Publication date
US9765379B2 (en) 2017-09-19
WO2013148249A1 (en) 2013-10-03
BR112014023176A2 (pt) 2017-06-20
ZA201406434B (en) 2017-08-30
US20240158825A1 (en) 2024-05-16
JP2019141049A (ja) 2019-08-29
CN104350156A (zh) 2015-02-11
JP2020202849A (ja) 2020-12-24
JP2015512259A (ja) 2015-04-27
PL2831261T3 (pl) 2018-04-30
US20140080180A1 (en) 2014-03-20
EP2831261A1 (en) 2015-02-04
MY171729A (en) 2019-10-25
CA3086730A1 (en) 2013-10-03
CA2866753A1 (en) 2013-10-03
BR112014023176B1 (pt) 2021-09-28
JP6225162B2 (ja) 2017-11-01
AU2019226192A1 (en) 2019-09-26
CN104350156B (zh) 2019-04-02
SI2831261T1 (en) 2018-01-31
IL234377B (en) 2021-01-31
AU2017202484B2 (en) 2019-06-13
US20230069966A1 (en) 2023-03-09
US20180291411A1 (en) 2018-10-11
US20160130624A1 (en) 2016-05-12
RU2636353C2 (ru) 2017-11-22
KR20140148392A (ko) 2014-12-31
ES2654424T3 (es) 2018-02-13
US20150225760A1 (en) 2015-08-13
MX365947B (es) 2019-06-19
AU2013240280A1 (en) 2014-10-16
RU2017139267A (ru) 2019-02-12
SG10201912877SA (en) 2020-02-27
SG10202007206WA (en) 2020-08-28
SG10201508420VA (en) 2015-11-27
AR116615A2 (es) 2021-05-26
AU2017202484A1 (en) 2017-05-04
KR20200037425A (ko) 2020-04-08
US20210171997A1 (en) 2021-06-10
KR102096678B1 (ko) 2020-04-02
HK1207120A1 (en) 2016-01-22
RU2014137554A (ru) 2016-05-20
MX2018007677A (es) 2020-11-12
SG11201406112TA (en) 2014-10-30
US20200199639A1 (en) 2020-06-25
MX2014011558A (es) 2014-11-14
EP2831261B1 (en) 2017-10-18
HRP20171966T1 (hr) 2018-02-09
CA2866753C (en) 2020-09-22
CN109880867A (zh) 2019-06-14
JP2018046825A (ja) 2018-03-29
MX2019007032A (es) 2019-08-16

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