MX2018007677A - Operaciones de cosecha mejoradas para proteinas recombinadas. - Google Patents

Operaciones de cosecha mejoradas para proteinas recombinadas.

Info

Publication number
MX2018007677A
MX2018007677A MX2018007677A MX2018007677A MX2018007677A MX 2018007677 A MX2018007677 A MX 2018007677A MX 2018007677 A MX2018007677 A MX 2018007677A MX 2018007677 A MX2018007677 A MX 2018007677A MX 2018007677 A MX2018007677 A MX 2018007677A
Authority
MX
Mexico
Prior art keywords
recombinant protein
host cell
prokaryotic host
filtered bulk
recombinant proteins
Prior art date
Application number
MX2018007677A
Other languages
English (en)
Inventor
Michael W Laird
John Richard St
Jane V Gunson
Kim Kaleas
Deepa Nadarajah
Rachel Adams
Bradley R Snedecor
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of MX2018007677A publication Critical patent/MX2018007677A/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/42Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención contempla un método para la producción de una proteína recombinada que comprende (a) la fermentación de una célula huésped procariota, donde dicha célula huésped procariota ha sido transformada con un ácido nucleico que codifica dicha proteína recombinada, y (b) la cosecha de dicha proteína recombinada en condiciones donde los niveles de dO2 son superiores a 0%, y (c) la purificación de dicha proteína recombinada hasta un volumen filtrado, donde dicho volumen filtrado no contiene una cantidad detectable de aducto de DHNA-proteína recombinada, medida por un ensayo de IEC a 310 nm. La presente invención además contempla un método para la producción de una proteína recombinada que comprende (a) la fermentación de una célula huésped procariota con deleción del gen menE, donde dicha célula huésped procariota ha sido transformada con un ácido nucleico que codifica dicha proteína recombinada, (b) la cosecha de dicha proteína recombinada; y (c) la purificación de dicha proteína recombinada hasta un volumen filtrado, donde dicho volumen filtrado no contiene una cantidad detectable de aducto de DHNA-proteína recombinada, medida por un ensayo de IEC a 310 nm, y donde el rendimiento de proteína recombinada se incrementa alrededor de 20% o más.
MX2018007677A 2012-03-27 2014-09-25 Operaciones de cosecha mejoradas para proteinas recombinadas. MX2018007677A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261616297P 2012-03-27 2012-03-27

Publications (1)

Publication Number Publication Date
MX2018007677A true MX2018007677A (es) 2020-11-12

Family

ID=48040433

Family Applications (3)

Application Number Title Priority Date Filing Date
MX2014011558A MX365947B (es) 2012-03-27 2013-03-14 Operaciones de cosecha mejoradas para proteinas recombinadas.
MX2018007677A MX2018007677A (es) 2012-03-27 2014-09-25 Operaciones de cosecha mejoradas para proteinas recombinadas.
MX2019007032A MX2019007032A (es) 2012-03-27 2014-09-25 Operaciones de cosecha mejoradas para proteinas recombinadas.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2014011558A MX365947B (es) 2012-03-27 2013-03-14 Operaciones de cosecha mejoradas para proteinas recombinadas.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2019007032A MX2019007032A (es) 2012-03-27 2014-09-25 Operaciones de cosecha mejoradas para proteinas recombinadas.

Country Status (21)

Country Link
US (8) US20140080180A1 (es)
EP (1) EP2831261B1 (es)
JP (4) JP6225162B2 (es)
KR (2) KR20200037425A (es)
CN (2) CN104350156B (es)
AR (2) AR090340A1 (es)
AU (3) AU2013240280A1 (es)
BR (1) BR112014023176B1 (es)
CA (2) CA3086730A1 (es)
ES (1) ES2654424T3 (es)
HK (1) HK1207120A1 (es)
HR (1) HRP20171966T1 (es)
IL (1) IL234377B (es)
MX (3) MX365947B (es)
MY (1) MY171729A (es)
PL (1) PL2831261T3 (es)
RU (2) RU2636353C2 (es)
SG (4) SG10201508420VA (es)
SI (1) SI2831261T1 (es)
WO (1) WO2013148249A1 (es)
ZA (1) ZA201406434B (es)

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EA201892820A1 (ru) 2016-06-10 2019-10-31 Способ стабилизации белков
TWI763732B (zh) 2016-11-07 2022-05-11 美商西雅圖遺傳學公司 工程化半胱胺酸帽之分佈
JP2021519298A (ja) * 2018-03-27 2021-08-10 ブリストル−マイヤーズ スクイブ カンパニーBristol−Myers Squibb Company 紫外線シグナルを使用した力価のリアルタイムモニタリング
CN109725088B (zh) * 2019-03-16 2021-05-18 丁立平 一种测定豆芽中2-萘甲酸及其衍生物的气相色谱-质谱联用法
CN110305903B (zh) * 2019-07-31 2021-10-01 江苏璟泽生物医药有限公司 重组人促卵泡激素及其制备方法
CN113155776A (zh) * 2021-04-29 2021-07-23 华东交通大学 一种柑桔最佳采收期预测方法

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Also Published As

Publication number Publication date
AU2017202484A1 (en) 2017-05-04
MY171729A (en) 2019-10-25
US20150225760A1 (en) 2015-08-13
CN104350156A (zh) 2015-02-11
SG10202007206WA (en) 2020-08-28
JP2019141049A (ja) 2019-08-29
EP2831261B1 (en) 2017-10-18
US20200199639A1 (en) 2020-06-25
AU2019226192A1 (en) 2019-09-26
CA3086730A1 (en) 2013-10-03
HK1207120A1 (en) 2016-01-22
US20180291411A1 (en) 2018-10-11
US20140080180A1 (en) 2014-03-20
SI2831261T1 (en) 2018-01-31
AR090340A1 (es) 2014-11-05
KR20140148392A (ko) 2014-12-31
PL2831261T3 (pl) 2018-04-30
JP6225162B2 (ja) 2017-11-01
MX2014011558A (es) 2014-11-14
JP2020202849A (ja) 2020-12-24
CA2866753A1 (en) 2013-10-03
MX365947B (es) 2019-06-19
CN109880867A (zh) 2019-06-14
RU2017139267A (ru) 2019-02-12
SG10201912877SA (en) 2020-02-27
US9765379B2 (en) 2017-09-19
CN104350156B (zh) 2019-04-02
AU2013240280A1 (en) 2014-10-16
HRP20171966T1 (hr) 2018-02-09
BR112014023176B1 (pt) 2021-09-28
US20210171997A1 (en) 2021-06-10
MX2019007032A (es) 2019-08-16
RU2636353C2 (ru) 2017-11-22
US20160130624A1 (en) 2016-05-12
EP2831261A1 (en) 2015-02-04
AR116615A2 (es) 2021-05-26
US20240158825A1 (en) 2024-05-16
ZA201406434B (en) 2017-08-30
AU2017202484B2 (en) 2019-06-13
SG11201406112TA (en) 2014-10-30
CA2866753C (en) 2020-09-22
JP2018046825A (ja) 2018-03-29
KR102096678B1 (ko) 2020-04-02
ES2654424T3 (es) 2018-02-13
JP2015512259A (ja) 2015-04-27
RU2014137554A (ru) 2016-05-20
US20230069966A1 (en) 2023-03-09
IL234377B (en) 2021-01-31
BR112014023176A2 (pt) 2017-06-20
WO2013148249A1 (en) 2013-10-03
SG10201508420VA (en) 2015-11-27
KR20200037425A (ko) 2020-04-08

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