WO2011000933A3 - Variants ameliores d'une phytase - Google Patents

Variants ameliores d'une phytase Download PDF

Info

Publication number
WO2011000933A3
WO2011000933A3 PCT/EP2010/059413 EP2010059413W WO2011000933A3 WO 2011000933 A3 WO2011000933 A3 WO 2011000933A3 EP 2010059413 W EP2010059413 W EP 2010059413W WO 2011000933 A3 WO2011000933 A3 WO 2011000933A3
Authority
WO
WIPO (PCT)
Prior art keywords
variant
phytase
relates
enhanced
field
Prior art date
Application number
PCT/EP2010/059413
Other languages
English (en)
Other versions
WO2011000933A2 (fr
Inventor
Stéphane BLESA
Hélène CHAUTARD
Marc Delcourt
Laurent Mesta
Bruno Winter
Original Assignee
Biomethodes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomethodes filed Critical Biomethodes
Priority to US13/380,694 priority Critical patent/US9109210B2/en
Priority to EP10737804A priority patent/EP2449100A2/fr
Priority to CN201080029857.6A priority patent/CN102597227B/zh
Priority to CA2766704A priority patent/CA2766704C/fr
Publication of WO2011000933A2 publication Critical patent/WO2011000933A2/fr
Publication of WO2011000933A3 publication Critical patent/WO2011000933A3/fr
Priority to HK13100502.6A priority patent/HK1173185A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03008—3-Phytase (3.1.3.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03026—4-Phytase (3.1.3.26), i.e. 6-phytase

Abstract

La présente invention relève du domaine de l'amélioration des protéines et en particulier celui des protéines améliorées par évolution moléculaire. Elle porte sur un variant d'une phytase, dit amélioré, en ce qu'il présente une thermostabilité et / ou une activité supérieures à la phytase d'origine. L'invention porte également sur un acide nucléique codant pour ce variant, une cassette ou un vecteur d'expression contenant ce variant, une cellule hôte exprimant ce variant, une composition comprenant ce variant ainsi que les utilisations de ceux-ci principalement dans la préparation d'additifs alimentaires et l'alimentation animale.
PCT/EP2010/059413 2009-07-01 2010-07-01 Variants ameliores d'une phytase WO2011000933A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US13/380,694 US9109210B2 (en) 2009-07-01 2010-07-01 Enhanced phytase variants
EP10737804A EP2449100A2 (fr) 2009-07-01 2010-07-01 Variants ameliores d'une phytase
CN201080029857.6A CN102597227B (zh) 2009-07-01 2010-07-01 增强的肌醇六磷酸酶变体
CA2766704A CA2766704C (fr) 2009-07-01 2010-07-01 Variants ameliores d'une phytase
HK13100502.6A HK1173185A1 (zh) 2009-07-01 2013-01-11 增强的肌醇六磷酸酶變體

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0954490 2009-07-01
FR0954490A FR2947563B1 (fr) 2009-07-01 2009-07-01 Variants ameliores d'une phytase

Publications (2)

Publication Number Publication Date
WO2011000933A2 WO2011000933A2 (fr) 2011-01-06
WO2011000933A3 true WO2011000933A3 (fr) 2011-03-17

Family

ID=41819699

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2010/059413 WO2011000933A2 (fr) 2009-07-01 2010-07-01 Variants ameliores d'une phytase

Country Status (7)

Country Link
US (1) US9109210B2 (fr)
EP (1) EP2449100A2 (fr)
CN (1) CN102597227B (fr)
CA (1) CA2766704C (fr)
FR (1) FR2947563B1 (fr)
HK (1) HK1173185A1 (fr)
WO (1) WO2011000933A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2553090T3 (pl) 2010-03-26 2019-08-30 Novozymes A/S Termostabilne warianty fitazy
WO2015070372A1 (fr) * 2013-11-12 2015-05-21 中国农业科学院饲料研究所 Procédé de production d'un variant de phytase présentant une stabilité thermique améliorée et variant de phytase et son utilisation
EP3608402A1 (fr) * 2018-08-08 2020-02-12 Clariant Produkte (Deutschland) GmbH 3-phytase optimisée et son utilisation dans la production d'aliments pour humains ou animaux
CN109750015B (zh) * 2019-03-27 2023-05-23 云南师范大学 一种热稳性提高的木聚糖酶突变体及其应用
CN114807088B (zh) * 2022-06-28 2022-09-27 中国农业科学院北京畜牧兽医研究所 一种提高植酸酶热稳定性的方法及突变体APPAmut6和应用
CN114807087B (zh) * 2022-06-28 2022-09-27 中国农业科学院北京畜牧兽医研究所 一种提高植酸酶热稳定性的方法及突变体和应用
CN114807089B (zh) * 2022-06-29 2022-09-27 中国农业科学院北京畜牧兽医研究所 一种提高植酸酶热稳定性的方法及突变体APPAmut7和应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007128160A1 (fr) * 2006-04-30 2007-11-15 Feed Research Institute Chinese Academy Of Agricultural Sciences Clonage et expression d'une nouvelle phytase

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2886943B1 (fr) * 2005-06-10 2007-09-07 Biomethodes Sa Methode de selection de proteines stables dans des conditions physico-chimiques non standard
JP5643083B2 (ja) * 2007-03-26 2014-12-17 ノボザイムス アクティーゼルスカブ ハフニア・フィターゼ

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007128160A1 (fr) * 2006-04-30 2007-11-15 Feed Research Institute Chinese Academy Of Agricultural Sciences Clonage et expression d'une nouvelle phytase

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DATABASE UniProt [online] 16 December 2008 (2008-12-16), "SubName: Full=Phytase;", XP002574150, retrieved from EBI accession no. UNIPROT:B6RGT1 Database accession no. B6RGT1 *
DATABASE UniProt [online] 23 September 2008 (2008-09-23), "SubName: Full=Phytase; EC=<A HREF="http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?[enzyme-ECNumber:3.1.3.26]+-e">3.1.3.26</A>;", XP002574169, retrieved from EBI accession no. UNIPROT:B4X9S4 Database accession no. B4X9S4 *
DATABASE UniProt [online] 6 February 2007 (2007-02-06), "SubName: Full=Probable histidine acid phosphatase; Flags: Precursor;", XP002574094, retrieved from EBI accession no. UNIPROT:A1JTE2 Database accession no. A1JTE2 *
FU ET AL: "A highly pH-stable phytase from Yersinia kristeensenii: Cloning, expression, and characterization", ENZYME AND MICROBIAL TECHNOLOGY, STONEHAM, MA, US, vol. 42, no. 6, 2 February 2008 (2008-02-02), pages 499 - 505, XP022573926, ISSN: 0141-0229 *
HUANG H ET AL: "A novel phytase with preferable characteristics from Yersinia intermedia", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 350, no. 4, 1 December 2006 (2006-12-01), pages 884 - 889, XP024924729, ISSN: 0006-291X, [retrieved on 20061201] *
HUOQING HUANG ET AL: "A novel phytase from Yersinia rohdei with high phytate hydrolysis activity under low pH and strong pepsin conditions", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 80, no. 3, 12 June 2008 (2008-06-12), pages 417 - 426, XP019623709, ISSN: 1432-0614 *
See also references of EP2449100A2 *

Also Published As

Publication number Publication date
CN102597227A (zh) 2012-07-18
CA2766704A1 (fr) 2011-01-06
EP2449100A2 (fr) 2012-05-09
US9109210B2 (en) 2015-08-18
US20130122567A1 (en) 2013-05-16
FR2947563B1 (fr) 2015-08-21
HK1173185A1 (zh) 2013-05-10
CA2766704C (fr) 2018-06-12
FR2947563A1 (fr) 2011-01-07
CN102597227B (zh) 2016-02-10
WO2011000933A2 (fr) 2011-01-06

Similar Documents

Publication Publication Date Title
WO2011000933A3 (fr) Variants ameliores d&#39;une phytase
PH12015500213A1 (en) Production of fmdv-resistant livestock by allele substitution
WO2013006953A8 (fr) Gènes et protéines pour la synthèse d&#39;alcanoyl-coa
EP3805348A3 (fr) Polypeptides avant une activite d activation cellulolytique et polynucleotides codant pour ceux-ci
MX2012003473A (es) Polipeptidos que tienen actividad celulitica mejorada y polinucleotidos que codifican para los mismos.
WO2011005867A8 (fr) Polypeptides ayant une activité cellulolytique améliorée et polynucléotides codant pour ceux-ci
MX2013007997A (es) Polipeptidos que tienen actividad de incremento celulolitico y polinucleotidos que codifican para los mismos.
EP2735611A3 (fr) Polypeptides dotés d&#39;activité d&#39;amélioration cellulolytique et polynucléotides les codant
WO2012146171A9 (fr) Polypeptides capables de favoriser l&#39;activité cellulolytique et polynucléotides codant pour ceux-ci
MX351916B (es) Polipeptidos que tienen actividad de lisozima y polinucleotdos que los codifican.
WO2013029013A8 (fr) Souches de bacillus produisant une enzyme
MX369726B (es) Polipéptidos con actividad proteasa.
MX337985B (es) Polipeptidos que tienen actividad de celobiohidrolasa y polinucleotidos que codifican los mismos.
MX337919B (es) Polipeptidos que tienen actividad de celobiohidrolasa y polinucleotidos que codifican los mismos.
MX362430B (es) Enzima de enfermedad del almacenamiento lisosomico.
MX2015013033A (es) Metodo para preparar harina de grano entero altamente dispersable con un contenido mejorado de avenantramida.
MX337913B (es) Polipeptidos que tienen actividad celobiohidrolasa y polinucleotidos que codifican los mismos.
MX2013003237A (es) Variantes de polipeptidos que tienen actividad potenciadora celulolitica y polinucleotidos que codifican a los mismos.
MX2013003236A (es) Variantes de polipeptidos que tienen actividad potenciadora celulolitica y polinucleotidos que codifican a los mismos.
MX358963B (es) Polipeptidos con actividad proteasa.
WO2011048046A3 (fr) Variantes de phytase synthétiques
NZ702681A (en) Yeast-containing silage inoculants for the enhancement of silage digestion and fermentation in the rumen
MX2014001594A (es) Polipeptidos que tienen actividad de celulasa y polinucleotidos que los codifican.
MX349517B (es) Polipeptidos que tienen actividad de proteasa y polinucleotidos que codifican los mismos.
WO2013189972A3 (fr) Utilisation de polypeptides ayant une activité protéase dans des aliments pour animaux et des détergents

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 201080029857.6

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 10737804

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 2574/MUMNP/2011

Country of ref document: IN

WWE Wipo information: entry into national phase

Ref document number: 2766704

Country of ref document: CA

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2010737804

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 13380694

Country of ref document: US