TR201907221T4 - G-csf üretimine yönelik yöntem. - Google Patents

G-csf üretimine yönelik yöntem. Download PDF

Info

Publication number
TR201907221T4
TR201907221T4 TR2019/07221T TR201907221T TR201907221T4 TR 201907221 T4 TR201907221 T4 TR 201907221T4 TR 2019/07221 T TR2019/07221 T TR 2019/07221T TR 201907221 T TR201907221 T TR 201907221T TR 201907221 T4 TR201907221 T4 TR 201907221T4
Authority
TR
Turkey
Prior art keywords
temperature
host cell
cultivation
recombinant polypeptide
csf
Prior art date
Application number
TR2019/07221T
Other languages
English (en)
Inventor
Felföldi Ferenc
Olasz Katalin
Kozma József
Original Assignee
Richter Gedeon Nyrt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Richter Gedeon Nyrt filed Critical Richter Gedeon Nyrt
Publication of TR201907221T4 publication Critical patent/TR201907221T4/tr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

İnklüzyon gövdelerinden rekombinant polipeptitlerin üretimi için yeni yöntemler açıklanmaktadır. Hücre kültür koşullarının modülasyonu, aktif formda rekombinant polipeptit verimini olumlu bir şekilde etkilemektedir. Bir yönde yöntem, (a) bir birinci sıcaklıkta söz konusu mikrobiyal bir konak hücrenin kültive edilmesini, konak hücre bir rekombinant polipeptidi kodlayan bir nükleik asit içermektedir, (b) kültivasyon sıcaklığının birinci sıcaklıktan bir ikinci sıcaklığa düşürülmesini, ve (c) ikinci sıcaklıkta konak hücrenin kültive edilmesini içermektedir.
TR2019/07221T 2012-03-19 2013-03-18 G-csf üretimine yönelik yöntem. TR201907221T4 (tr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
HU1200171A HUP1200171A1 (hu) 2012-03-19 2012-03-19 Módszerek polipeptidek elõállítására

Publications (1)

Publication Number Publication Date
TR201907221T4 true TR201907221T4 (tr) 2019-06-21

Family

ID=89990648

Family Applications (1)

Application Number Title Priority Date Filing Date
TR2019/07221T TR201907221T4 (tr) 2012-03-19 2013-03-18 G-csf üretimine yönelik yöntem.

Country Status (15)

Country Link
US (1) US9416164B2 (tr)
EP (2) EP2828396B1 (tr)
JP (1) JP6154885B2 (tr)
KR (1) KR101949556B1 (tr)
CN (1) CN104603280A (tr)
CA (1) CA2867794C (tr)
DK (1) DK2828396T3 (tr)
ES (1) ES2732374T3 (tr)
HR (1) HRP20190846T1 (tr)
HU (2) HUP1200171A1 (tr)
LT (1) LT2828396T (tr)
PL (1) PL2828396T3 (tr)
SI (1) SI2828396T1 (tr)
TR (1) TR201907221T4 (tr)
WO (1) WO2013068602A2 (tr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUP1200172A2 (en) 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
HUP1200171A1 (hu) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására
JP6697383B2 (ja) * 2014-06-09 2020-05-20 株式会社カネカ 組換えブレビバチルス属細菌を用いた組換え蛋白質の製造方法
EP3902820A2 (en) * 2018-12-28 2021-11-03 Coherus Biosciences, Inc. Process for producing, isolating, and purifying modified recombinant proteins
WO2020234742A1 (en) * 2019-05-20 2020-11-26 Lupin Limited Granulocyte colony stimulating factor purification

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61227526A (ja) 1984-07-25 1986-10-09 Chugai Pharmaceut Co Ltd 新規なコロニー刺激因子
US4810643A (en) 1985-08-23 1989-03-07 Kirin- Amgen Inc. Production of pluripotent granulocyte colony-stimulating factor
DE3537708A1 (de) 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
JPH0618780B2 (ja) * 1986-01-22 1994-03-16 中外製薬株式会社 骨髄性白血病抑制剤
US5714581A (en) 1986-12-23 1998-02-03 Kyowa Hakko Kogyo Co., Ltd. Polypeptide derivatives of human granulocyte colony stimulating factor
EP0719860B1 (en) 1988-05-13 2009-12-16 Amgen Inc. Process for isolating and purifying G-CSF
DE3835350A1 (de) 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
CA2006596C (en) 1988-12-22 2000-09-05 Rika Ishikawa Chemically-modified g-csf
US5055555A (en) 1989-01-05 1991-10-08 Helmut Sassenfeld Purification of G-CSF
FR2669646A1 (fr) 1990-11-23 1992-05-29 Lorraine Laminage Procede de traitement d'un effluent aqueux issu d'un procede d'electrozingage, ainsi que procede et installation en comportant application.
DE4114575C1 (tr) 1991-05-04 1992-05-14 Fa. Carl Freudenberg, 6940 Weinheim, De
KR100241257B1 (ko) 1997-05-22 2000-02-01 한승수 인체 과립구 콜로니 자극 인자 활성물질 및 그것의 제조방법
AU4975699A (en) 1998-07-09 2000-02-01 University Technology Corporation High pressure refolding of protein aggregates and inclusion bodies
FR2796071B1 (fr) 1999-07-08 2001-09-07 Hoechst Marion Roussel Inc Procede de purification de facteur de stimulation de colonies de granulocytes
EP1284987B1 (en) 2000-05-16 2007-07-18 Bolder Biotechnology, Inc. Methods for refolding proteins containing free cysteine residues
SI21102A (sl) 2001-12-19 2003-06-30 LEK, tovarna farmacevtskih in kemi�nih izdelkov, d.d. Postopek za izolacijo biološko aktivnega granulocitne kolonije stimulirajočega dejavnika
AU2002345829A1 (en) 2002-06-24 2004-01-06 Dr. Reddy's Laboratories Ltd. Process for preparing g-csf
SI21273A (sl) 2002-07-31 2004-02-29 LEK farmacevtska dru�ba d.d. Priprava inkluzijskih teles z visokim deležem pravilno zvitega prekurzorja heterolognega proteina
KR100484653B1 (ko) * 2004-05-06 2005-04-20 주식회사 대웅 원핵세포에서 활성형의 가용성 단백질을 제조하는 방법 및 이를 위한 폴리시스트론 벡터
DE102004041639A1 (de) 2004-08-27 2006-03-02 Bioceuticals Arzneimittel Ag Verfahren zur Gewinnung von biologisch aktivem humanen G-CSF aus Inclusion Bodies
EP1869078A2 (en) 2005-03-17 2007-12-26 Zenotech Laboratories Limited Process for the purification of recombinant granulocyte-colony stimulating factor
KR100694994B1 (ko) 2005-06-13 2007-03-14 씨제이 주식회사 사람 과립구 콜로니 형성인자 동종체
GB0605684D0 (en) 2006-03-21 2006-05-03 Sicor Biotech Uab Method For Purifying Granulocyte-Colony Stimulating Factor
WO2008009368A1 (en) 2006-07-21 2008-01-24 Sony Service Centre (Europe) N.V. System and method of prioritising tasks
WO2008096370A2 (en) 2007-02-05 2008-08-14 Natco Pharma Limited An efficient and novel purification method of recombinant hg-csf
WO2008096368A2 (en) * 2007-02-05 2008-08-14 Natco Pharma Limited A novel process for production of recombinant human g-csf
CN101358196A (zh) 2008-08-29 2009-02-04 浙江理工大学 利用家蚕后部丝腺体外表达系统合成蛋白质的方法
CN101691560B (zh) 2008-12-19 2011-08-31 华南理工大学 大肠杆菌及其可溶性表达转谷氨酰胺酶酶原的方法
JP2012530131A (ja) 2009-06-16 2012-11-29 ルピン・リミテッド 組み換えヒト顆粒球コロニー刺激因子の精製のための方法
HUP1200171A1 (hu) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására
HUP1200172A2 (en) 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies

Also Published As

Publication number Publication date
JP2015514398A (ja) 2015-05-21
EP2828396B1 (en) 2019-04-24
KR20140144705A (ko) 2014-12-19
ES2732374T3 (es) 2019-11-22
KR101949556B1 (ko) 2019-02-18
HUP1200171A1 (hu) 2013-09-30
CA2867794A1 (en) 2013-05-16
EP2828396A2 (en) 2015-01-28
SI2828396T1 (sl) 2019-06-28
JP6154885B2 (ja) 2017-06-28
WO2013068602A2 (en) 2013-05-16
HUE045310T2 (hu) 2019-12-30
CN104603280A (zh) 2015-05-06
DK2828396T3 (da) 2019-05-27
US20150044721A1 (en) 2015-02-12
LT2828396T (lt) 2019-05-27
HRP20190846T1 (hr) 2019-08-09
EP3517621A1 (en) 2019-07-31
WO2013068602A3 (en) 2013-08-01
CA2867794C (en) 2020-06-09
PL2828396T3 (pl) 2019-08-30
US9416164B2 (en) 2016-08-16

Similar Documents

Publication Publication Date Title
BR112018004377A2 (pt) cepas de levedura para a expressão e secreção de proteínas heterólogas em temperaturas elevadas
CY1118863T1 (el) Καλλιεργεια κυτταρου θηλαστικου
MX2019004568A (es) Produccion mejorada de derivados de acidos grasos.
MX2019003427A (es) Composiciones para cultivo celular y metodos para la produccion de polipeptidos.
TR201907221T4 (tr) G-csf üretimine yönelik yöntem.
WO2011134919A3 (en) Cho cells cultivation process 0
EP3722438A3 (en) Method for production of moth pheromones in yeast
EA201500142A1 (ru) ШТАММ, ДЕФИЦИТНЫЙ ПО AgsE
BR112021021777A2 (pt) Métodos para a produção de ergotioneína
IN2014DN03419A (tr)
IN2014CN03210A (tr)
EP3916081A3 (en) Control of protein glycosylation by culture medium supplementation and cell culture process parameters
MX2015012875A (es) Composiciones de cultivo celular con antioxidantes y metodos para la produccion de polipeptidos.
BR112014015228A8 (pt) variante de celobiohidrolase, polinucleotídeo isolado, método de produção de uma variante, planta, parte de planta ou célula de planta transgênica, método para obtenção de uma variante de celobiohidrolase, processos para degradação ou conversão de um material celulósico, para produção de um produto de fermentação, e de fermentação de um material celulósico, formulação ou composição cultura celular de caldo inteiro, e, célula hospedeira microbiana transgênica microbiana transgênica
MX2016008258A (es) Celulas eucariotas novedosas y metodos para la expresion recombinante de un producto de interes.
MX368656B (es) Métodos mejorados para la producción de polipéptidos recombinantes.
MX2013009855A (es) Plantas que tienen rasgos relacionados con rendimiento mejorado y metodos para producir los mismos.
MX2020001357A (es) Produccion microbiana de aminas grasas.
MX2013012986A (es) Plantas que tienen uno o mas rasgos mejorados relacionados con el rendimento y un metodo para producirlas.
WO2013032999A3 (en) Polydnavirus vectors
WO2011085095A3 (en) Enhanced protein expression
WO2015077538A8 (en) Nucleotide sequence encoding homeobox-leucine zipper protein hat22 (hd-zip protein 22) from corchorus olitorius and corchorus capsularis and methods of use
EA201591723A1 (ru) Применение термофильных нуклеаз для разрушения нуклеиновых кислот
MX2013011188A (es) Metodos y materiales de ingenieria genetica para aumentar el rendimiento de las plantas.
WO2015117033A3 (en) Methods of increasing protein production in mammalian cells