RU2017139267A - Усовершенствованные способы выделения рекомбинантных белков - Google Patents
Усовершенствованные способы выделения рекомбинантных белков Download PDFInfo
- Publication number
- RU2017139267A RU2017139267A RU2017139267A RU2017139267A RU2017139267A RU 2017139267 A RU2017139267 A RU 2017139267A RU 2017139267 A RU2017139267 A RU 2017139267A RU 2017139267 A RU2017139267 A RU 2017139267A RU 2017139267 A RU2017139267 A RU 2017139267A
- Authority
- RU
- Russia
- Prior art keywords
- level
- recombinant protein
- host cell
- prokaryotic host
- homogenization
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims 21
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title claims 13
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title claims 13
- 238000000265 homogenisation Methods 0.000 claims 5
- 238000004255 ion exchange chromatography Methods 0.000 claims 4
- 238000002156 mixing Methods 0.000 claims 3
- 238000002360 preparation method Methods 0.000 claims 3
- VOJUXHHACRXLTD-UHFFFAOYSA-M 1,4-dihydroxy-2-naphthoate Chemical compound C1=CC=C2C(O)=CC(C([O-])=O)=C(O)C2=C1 VOJUXHHACRXLTD-UHFFFAOYSA-M 0.000 claims 2
- 108010065780 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase Proteins 0.000 claims 2
- 241000588724 Escherichia coli Species 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 108010089355 dihydroneopterin aldolase Proteins 0.000 claims 2
- 238000004128 high performance liquid chromatography Methods 0.000 claims 2
- 238000002955 isolation Methods 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 238000000746 purification Methods 0.000 claims 2
- VOJUXHHACRXLTD-UHFFFAOYSA-N 1,4-dihydroxy-2-naphthoic acid Chemical compound C1=CC=CC2=C(O)C(C(=O)O)=CC(O)=C21 VOJUXHHACRXLTD-UHFFFAOYSA-N 0.000 claims 1
- 241000589151 Azotobacter Species 0.000 claims 1
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 241000588914 Enterobacter Species 0.000 claims 1
- 241000588698 Erwinia Species 0.000 claims 1
- 241000588748 Klebsiella Species 0.000 claims 1
- 238000005481 NMR spectroscopy Methods 0.000 claims 1
- 241001057811 Paracoccus <mealybug> Species 0.000 claims 1
- 241000588769 Proteus <enterobacteria> Species 0.000 claims 1
- 241000589516 Pseudomonas Species 0.000 claims 1
- 241000607142 Salmonella Species 0.000 claims 1
- 241000607720 Serratia Species 0.000 claims 1
- 241000607768 Shigella Species 0.000 claims 1
- 241000863000 Vitreoscilla Species 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims 1
- 238000012217 deletion Methods 0.000 claims 1
- 230000037430 deletion Effects 0.000 claims 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 1
- 238000004949 mass spectrometry Methods 0.000 claims 1
- 101150087021 menE gene Proteins 0.000 claims 1
- 229910052760 oxygen Inorganic materials 0.000 claims 1
- 239000001301 oxygen Substances 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 238000004007 reversed phase HPLC Methods 0.000 claims 1
- 238000003860 storage Methods 0.000 claims 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Sustainable Development (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261616297P | 2012-03-27 | 2012-03-27 | |
| US61/616,297 | 2012-03-27 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2014137554A Division RU2636353C2 (ru) | 2012-03-27 | 2013-03-14 | Усовершенствованные способы выделения рекобинантных белков |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2017139267A true RU2017139267A (ru) | 2019-02-12 |
Family
ID=48040433
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2017139267A RU2017139267A (ru) | 2012-03-27 | 2013-03-14 | Усовершенствованные способы выделения рекомбинантных белков |
| RU2014137554A RU2636353C2 (ru) | 2012-03-27 | 2013-03-14 | Усовершенствованные способы выделения рекобинантных белков |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2014137554A RU2636353C2 (ru) | 2012-03-27 | 2013-03-14 | Усовершенствованные способы выделения рекобинантных белков |
Country Status (20)
| Country | Link |
|---|---|
| US (9) | US20140080180A1 (enExample) |
| EP (1) | EP2831261B1 (enExample) |
| JP (4) | JP6225162B2 (enExample) |
| KR (2) | KR102096678B1 (enExample) |
| CN (2) | CN109880867A (enExample) |
| AR (2) | AR090340A1 (enExample) |
| AU (3) | AU2013240280A1 (enExample) |
| BR (1) | BR112014023176B1 (enExample) |
| CA (2) | CA3086730A1 (enExample) |
| ES (1) | ES2654424T3 (enExample) |
| HR (1) | HRP20171966T1 (enExample) |
| IL (1) | IL234377B (enExample) |
| MX (3) | MX365947B (enExample) |
| MY (1) | MY171729A (enExample) |
| PL (1) | PL2831261T3 (enExample) |
| RU (2) | RU2017139267A (enExample) |
| SG (4) | SG11201406112TA (enExample) |
| SI (1) | SI2831261T1 (enExample) |
| WO (1) | WO2013148249A1 (enExample) |
| ZA (1) | ZA201406434B (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6201037B1 (en) | 1986-01-28 | 2001-03-13 | Ophthalmic Research Group International, Inc. | Plastic lens composition and method for the production thereof |
| JP2019517272A (ja) * | 2016-06-10 | 2019-06-24 | ロンザ リミテッドＬｏｎｚａ Ｌｉｍｉｔｅｄ | タンパク質の安定化方法 |
| KR102595048B1 (ko) * | 2016-11-07 | 2023-10-26 | 씨젠 인크. | 조작된-시스테인 캡의 분포 |
| US20210024873A1 (en) * | 2018-03-27 | 2021-01-28 | Bristol-Myers Squibb Company | Real-time monitoring of protein concentration using ultraviolet signal |
| FI3857237T3 (fi) * | 2019-01-16 | 2023-05-03 | Regeneron Pharma | Disulfidisidosten luonnehtimismenetelmiä |
| CN109725088B (zh) * | 2019-03-16 | 2021-05-18 | 丁立平 | 一种测定豆芽中2-萘甲酸及其衍生物的气相色谱-质谱联用法 |
| CN110305903B (zh) * | 2019-07-31 | 2021-10-01 | 江苏璟泽生物医药有限公司 | 重组人促卵泡激素及其制备方法 |
| CN113155776A (zh) * | 2021-04-29 | 2021-07-23 | 华东交通大学 | 一种柑桔最佳采收期预测方法 |
| FR3146146A1 (fr) | 2023-02-24 | 2024-08-30 | Bgene Genetics | Production d’acide 1-4-dihydroxy-2-naphtoïque par Escherichia coli |
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| ZA811368B (en) | 1980-03-24 | 1982-04-28 | Genentech Inc | Bacterial polypedtide expression employing tryptophan promoter-operator |
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-
2013
- 2013-03-14 SG SG11201406112TA patent/SG11201406112TA/en unknown
- 2013-03-14 CN CN201910197577.9A patent/CN109880867A/zh active Pending
- 2013-03-14 RU RU2017139267A patent/RU2017139267A/ru not_active Application Discontinuation
- 2013-03-14 ES ES13713295.7T patent/ES2654424T3/es active Active
- 2013-03-14 AR ARP130100828A patent/AR090340A1/es active IP Right Grant
- 2013-03-14 AU AU2013240280A patent/AU2013240280A1/en not_active Abandoned
- 2013-03-14 KR KR1020147026772A patent/KR102096678B1/ko active Active
- 2013-03-14 CA CA3086730A patent/CA3086730A1/en not_active Abandoned
- 2013-03-14 JP JP2015503300A patent/JP6225162B2/ja active Active
- 2013-03-14 EP EP13713295.7A patent/EP2831261B1/en active Active
- 2013-03-14 BR BR112014023176-1A patent/BR112014023176B1/pt active IP Right Grant
- 2013-03-14 MX MX2014011558A patent/MX365947B/es active IP Right Grant
- 2013-03-14 PL PL13713295T patent/PL2831261T3/pl unknown
- 2013-03-14 SG SG10201508420VA patent/SG10201508420VA/en unknown
- 2013-03-14 SI SI201330894T patent/SI2831261T1/en unknown
- 2013-03-14 SG SG10202007206WA patent/SG10202007206WA/en unknown
- 2013-03-14 CN CN201380027623.1A patent/CN104350156B/zh active Active
- 2013-03-14 MY MYPI2014002775A patent/MY171729A/en unknown
- 2013-03-14 HR HRP20171966TT patent/HRP20171966T1/hr unknown
- 2013-03-14 CA CA2866753A patent/CA2866753C/en active Active
- 2013-03-14 KR KR1020207008828A patent/KR20200037425A/ko not_active Ceased
- 2013-03-14 SG SG10201912877SA patent/SG10201912877SA/en unknown
- 2013-03-14 US US13/826,166 patent/US20140080180A1/en not_active Abandoned
- 2013-03-14 WO PCT/US2013/031383 patent/WO2013148249A1/en not_active Ceased
- 2013-03-14 RU RU2014137554A patent/RU2636353C2/ru active
-
2014
- 2014-08-31 IL IL234377A patent/IL234377B/en active IP Right Grant
- 2014-09-02 ZA ZA2014/06434A patent/ZA201406434B/en unknown
- 2014-09-25 MX MX2018007677A patent/MX2018007677A/es unknown
- 2014-09-25 MX MX2019007032A patent/MX2019007032A/es unknown
- 2014-09-26 US US14/497,964 patent/US9765379B2/en active Active
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2015
- 2015-06-09 US US14/734,848 patent/US20160130624A1/en not_active Abandoned
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2017
- 2017-04-13 AU AU2017202484A patent/AU2017202484B2/en active Active
- 2017-10-05 JP JP2017195016A patent/JP2018046825A/ja active Pending
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2018
- 2018-02-28 US US15/908,526 patent/US20180291411A1/en not_active Abandoned
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2019
- 2019-02-07 US US16/270,494 patent/US20200199639A1/en not_active Abandoned
- 2019-03-18 JP JP2019049717A patent/JP2019141049A/ja not_active Withdrawn
- 2019-09-05 AU AU2019226192A patent/AU2019226192A1/en not_active Abandoned
- 2019-10-09 AR ARP190102873A patent/AR116615A2/es unknown
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2020
- 2020-08-14 JP JP2020137053A patent/JP2020202849A/ja not_active Withdrawn
- 2020-10-09 US US17/067,059 patent/US20210171997A1/en not_active Abandoned
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2022
- 2022-07-13 US US17/864,255 patent/US20230069966A1/en not_active Abandoned
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2024
- 2024-01-22 US US18/419,368 patent/US20240158825A1/en not_active Abandoned
- 2024-12-20 US US18/990,600 patent/US20250137027A1/en active Pending
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