SI2831261T1 - A process for the production of low-level recombinant proteins DHNA (1,4-dihydroxy-2-naphthoate) - Google Patents
A process for the production of low-level recombinant proteins DHNA (1,4-dihydroxy-2-naphthoate) Download PDFInfo
- Publication number
- SI2831261T1 SI2831261T1 SI201330894T SI201330894T SI2831261T1 SI 2831261 T1 SI2831261 T1 SI 2831261T1 SI 201330894 T SI201330894 T SI 201330894T SI 201330894 T SI201330894 T SI 201330894T SI 2831261 T1 SI2831261 T1 SI 2831261T1
- Authority
- SI
- Slovenia
- Prior art keywords
- recombinant protein
- dhna
- level
- naphthoate
- dihydroxy
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title claims abstract 17
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title claims abstract 17
- VOJUXHHACRXLTD-UHFFFAOYSA-M 1,4-dihydroxy-2-naphthoate Chemical compound C1=CC=C2C(O)=CC(C([O-])=O)=C(O)C2=C1 VOJUXHHACRXLTD-UHFFFAOYSA-M 0.000 title claims 4
- 108010065780 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase Proteins 0.000 title claims 4
- 108010089355 dihydroneopterin aldolase Proteins 0.000 title claims 4
- VOJUXHHACRXLTD-UHFFFAOYSA-N 1,4-dihydroxy-2-naphthoic acid Chemical compound C1=CC=CC2=C(O)C(C(=O)O)=CC(O)=C21 VOJUXHHACRXLTD-UHFFFAOYSA-N 0.000 title claims 3
- 238000004519 manufacturing process Methods 0.000 title claims 3
- 238000000034 method Methods 0.000 claims abstract 14
- 102000039446 nucleic acids Human genes 0.000 claims abstract 3
- 108020004707 nucleic acids Proteins 0.000 claims abstract 3
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 3
- 238000000265 homogenisation Methods 0.000 claims 4
- 239000011248 coating agent Substances 0.000 claims 3
- 238000000576 coating method Methods 0.000 claims 3
- 238000002156 mixing Methods 0.000 claims 3
- 241000588724 Escherichia coli Species 0.000 claims 2
- 238000004128 high performance liquid chromatography Methods 0.000 claims 2
- 238000004255 ion exchange chromatography Methods 0.000 claims 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 239000013049 sediment Substances 0.000 claims 2
- 238000005481 NMR spectroscopy Methods 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 1
- 230000002045 lasting effect Effects 0.000 claims 1
- 238000004949 mass spectrometry Methods 0.000 claims 1
- 229910052760 oxygen Inorganic materials 0.000 claims 1
- 239000001301 oxygen Substances 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 claims 1
- 238000004007 reversed phase HPLC Methods 0.000 claims 1
- 230000002459 sustained effect Effects 0.000 claims 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 claims 1
- 238000003556 assay Methods 0.000 abstract 2
- 238000003306 harvesting Methods 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 241001214257 Mene Species 0.000 abstract 1
- 101150087021 menE gene Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Sustainable Development (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261616297P | 2012-03-27 | 2012-03-27 | |
| PCT/US2013/031383 WO2013148249A1 (en) | 2012-03-27 | 2013-03-14 | Improved harvest operations for recombinant proteins |
| EP13713295.7A EP2831261B1 (en) | 2012-03-27 | 2013-03-14 | Method for producing recombinant proteins with low levels of DHNA (1,4-dihydroxy-2-naphthoate) |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SI2831261T1 true SI2831261T1 (en) | 2018-01-31 |
Family
ID=48040433
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SI201330894T SI2831261T1 (en) | 2012-03-27 | 2013-03-14 | A process for the production of low-level recombinant proteins DHNA (1,4-dihydroxy-2-naphthoate) |
Country Status (20)
| Country | Link |
|---|---|
| US (9) | US20140080180A1 (enExample) |
| EP (1) | EP2831261B1 (enExample) |
| JP (4) | JP6225162B2 (enExample) |
| KR (2) | KR102096678B1 (enExample) |
| CN (2) | CN109880867A (enExample) |
| AR (2) | AR090340A1 (enExample) |
| AU (3) | AU2013240280A1 (enExample) |
| BR (1) | BR112014023176B1 (enExample) |
| CA (2) | CA3086730A1 (enExample) |
| ES (1) | ES2654424T3 (enExample) |
| HR (1) | HRP20171966T1 (enExample) |
| IL (1) | IL234377B (enExample) |
| MX (3) | MX365947B (enExample) |
| MY (1) | MY171729A (enExample) |
| PL (1) | PL2831261T3 (enExample) |
| RU (2) | RU2017139267A (enExample) |
| SG (4) | SG11201406112TA (enExample) |
| SI (1) | SI2831261T1 (enExample) |
| WO (1) | WO2013148249A1 (enExample) |
| ZA (1) | ZA201406434B (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6201037B1 (en) | 1986-01-28 | 2001-03-13 | Ophthalmic Research Group International, Inc. | Plastic lens composition and method for the production thereof |
| JP2019517272A (ja) * | 2016-06-10 | 2019-06-24 | ロンザ リミテッドＬｏｎｚａ Ｌｉｍｉｔｅｄ | タンパク質の安定化方法 |
| KR102595048B1 (ko) * | 2016-11-07 | 2023-10-26 | 씨젠 인크. | 조작된-시스테인 캡의 분포 |
| US20210024873A1 (en) * | 2018-03-27 | 2021-01-28 | Bristol-Myers Squibb Company | Real-time monitoring of protein concentration using ultraviolet signal |
| FI3857237T3 (fi) * | 2019-01-16 | 2023-05-03 | Regeneron Pharma | Disulfidisidosten luonnehtimismenetelmiä |
| CN109725088B (zh) * | 2019-03-16 | 2021-05-18 | 丁立平 | 一种测定豆芽中2-萘甲酸及其衍生物的气相色谱-质谱联用法 |
| CN110305903B (zh) * | 2019-07-31 | 2021-10-01 | 江苏璟泽生物医药有限公司 | 重组人促卵泡激素及其制备方法 |
| CN113155776A (zh) * | 2021-04-29 | 2021-07-23 | 华东交通大学 | 一种柑桔最佳采收期预测方法 |
| FR3146146A1 (fr) | 2023-02-24 | 2024-08-30 | Bgene Genetics | Production d’acide 1-4-dihydroxy-2-naphtoïque par Escherichia coli |
Family Cites Families (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2036891B (en) | 1978-12-05 | 1983-05-05 | Windsor Smith C | Change speed gear |
| US4515893A (en) | 1979-04-26 | 1985-05-07 | Ortho Pharmaceutical Corporation | Hybrid cell line for producing complement-fixing monoclonal antibody to human T cells |
| ZA811368B (en) | 1980-03-24 | 1982-04-28 | Genentech Inc | Bacterial polypedtide expression employing tryptophan promoter-operator |
| GB8308235D0 (en) | 1983-03-25 | 1983-05-05 | Celltech Ltd | Polypeptides |
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| US5672347A (en) | 1984-07-05 | 1997-09-30 | Genentech, Inc. | Tumor necrosis factor antagonists and their use |
| JPS6147500A (ja) | 1984-08-15 | 1986-03-07 | Res Dev Corp Of Japan | キメラモノクロ−ナル抗体及びその製造法 |
| EP0173494A3 (en) | 1984-08-27 | 1987-11-25 | The Board Of Trustees Of The Leland Stanford Junior University | Chimeric receptors by dna splicing and expression |
| GB8422238D0 (en) | 1984-09-03 | 1984-10-10 | Neuberger M S | Chimeric proteins |
| JPS61134325A (ja) | 1984-12-04 | 1986-06-21 | Teijin Ltd | ハイブリツド抗体遺伝子の発現方法 |
| WO1987002671A1 (en) | 1985-11-01 | 1987-05-07 | International Genetic Engineering, Inc. | Modular assembly of antibody genes, antibodies prepared thereby and use |
| US5225539A (en) | 1986-03-27 | 1993-07-06 | Medical Research Council | Recombinant altered antibodies and methods of making altered antibodies |
| US4946778A (en) | 1987-09-21 | 1990-08-07 | Genex Corporation | Single polypeptide chain binding molecules |
| IL85035A0 (en) | 1987-01-08 | 1988-06-30 | Int Genetic Eng | Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same |
| US5091313A (en) | 1988-08-05 | 1992-02-25 | Tanox Biosystems, Inc. | Antigenic epitopes of IgE present on B cell but not basophil surface |
| US5720937A (en) | 1988-01-12 | 1998-02-24 | Genentech, Inc. | In vivo tumor detection assay |
| WO1989012463A1 (en) | 1988-06-21 | 1989-12-28 | Genentech, Inc. | Method and therapeutic compositions for the treatment of myocardial infarction |
| US5530101A (en) | 1988-12-28 | 1996-06-25 | Protein Design Labs, Inc. | Humanized immunoglobulins |
| ES2193136T3 (es) | 1991-08-14 | 2003-11-01 | Genentech Inc | Variantes de inmunoglubina para receptores especificos de fc epsilon. |
| WO1993008829A1 (en) | 1991-11-04 | 1993-05-13 | The Regents Of The University Of California | Compositions that mediate killing of hiv-infected cells |
| US5288931A (en) | 1991-12-06 | 1994-02-22 | Genentech, Inc. | Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation |
| ATE149570T1 (de) | 1992-08-17 | 1997-03-15 | Genentech Inc | Bispezifische immunoadhesine |
| EP0656789B1 (en) | 1992-08-21 | 1997-12-17 | Genentech, Inc. | Method for treating a lfa-1-mediated disorder |
| US5736137A (en) | 1992-11-13 | 1998-04-07 | Idec Pharmaceuticals Corporation | Therapeutic application of chimeric and radiolabeled antibodies to human B lymphocyte restricted differentiation antigen for treatment of B cell lymphoma |
| US5714338A (en) | 1993-12-10 | 1998-02-03 | Genentech, Inc. | Methods for diagnosis of allergy |
| EP0739214B1 (en) | 1994-01-18 | 1998-03-18 | Genentech, Inc. | A METHOD OF TREATMENT OF PARASITIC INFECTION USING IgE ANTAGONISTS |
| EP0749488A1 (en) | 1994-03-03 | 1996-12-27 | Genentech, Inc. | Anti-il-8 monoclonal antibodies for treatment of inflammatory disorders |
| US5639635A (en) | 1994-11-03 | 1997-06-17 | Genentech, Inc. | Process for bacterial production of polypeptides |
| IL117645A (en) | 1995-03-30 | 2005-08-31 | Genentech Inc | Vascular endothelial cell growth factor antagonists for use as medicaments in the treatment of age-related macular degeneration |
| AU6267896A (en) | 1995-06-07 | 1996-12-30 | Imclone Systems Incorporated | Antibody and antibody fragments for inhibiting the growth oftumors |
| EP1297847A3 (en) | 1996-01-23 | 2003-05-07 | Genentech, Inc. | Anti-CD18 antibodies in stroke |
| US7147851B1 (en) | 1996-08-15 | 2006-12-12 | Millennium Pharmaceuticals, Inc. | Humanized immunoglobulin reactive with α4β7 integrin |
| KR100532178B1 (ko) | 1996-11-27 | 2005-12-01 | 제넨테크, 인크. | 인간화 항-ＣＤ11ａ 항체 |
| ES2236634T3 (es) | 1997-04-07 | 2005-07-16 | Genentech, Inc. | Anticuerpos anti-vegf. |
| ES2293682T5 (es) | 1997-05-15 | 2011-11-17 | Genentech, Inc. | Anticuerpo anti-apo2. |
| US6946129B1 (en) | 1999-06-08 | 2005-09-20 | Seattle Genetics, Inc. | Recombinant anti-CD40 antibody and uses thereof |
| DK1226177T3 (da) | 1999-10-29 | 2008-10-06 | Genentech Inc | Antistofsammensætninger mod anti-prostata stamcelle-antigen (PSCA) og anvendelser deraf |
| US6946271B2 (en) * | 2000-09-20 | 2005-09-20 | Degussa Ag | Nucleotide sequences which code for the menE gene |
| US6979556B2 (en) | 2000-12-14 | 2005-12-27 | Genentech, Inc. | Separate-cistron contructs for secretion of aglycosylated antibodies from prokaryotes |
| PT1572744E (pt) | 2002-12-16 | 2010-09-07 | Genentech Inc | Variantes de imunoglobulina e utilizações destas |
| KR101003344B1 (ko) * | 2003-03-26 | 2010-12-23 | 메이지 데어리스 코포레이션 | 1,4-디히드록시-2-나프토산의 안정화 방법 |
| US7785830B2 (en) * | 2003-08-13 | 2010-08-31 | Sandoz Ag | Expression vectors, transformed host cells and fermentation process for the production of recombinant polypeptides |
| WO2005078115A1 (en) * | 2004-02-04 | 2005-08-25 | Merck & Co., Inc. | Process for large scale production of plasmid dna by e. coli fermentation |
| US7361764B2 (en) * | 2004-07-27 | 2008-04-22 | Sgx Pharmaceuticals, Inc. | Pyrrolo-pyridine kinase modulators |
| CN101115832A (zh) * | 2004-11-26 | 2008-01-30 | 协和发酵工业株式会社 | 工业上有用的微生物 |
| JP5383481B2 (ja) * | 2006-05-05 | 2014-01-08 | ガンガゲン インコーポレーテッド | ファージに由来する抗微生物活性 |
| JP5305615B2 (ja) * | 2006-06-05 | 2013-10-02 | 株式会社Ａｄｅｋａ | メナキノンの新規生合成経路に関与するタンパク質の遺伝子群を利用したメナキノンの製造方法 |
| WO2008008975A2 (en) * | 2006-07-14 | 2008-01-17 | Genentech, Inc. | Refolding of recombinant proteins |
| WO2009009523A2 (en) * | 2007-07-09 | 2009-01-15 | Genentech, Inc. | Prevention of disulfide bond reduction during recombinant production of polypeptides |
| CN101665798B (zh) * | 2008-09-06 | 2012-11-21 | 浙江我武生物科技股份有限公司 | 一种制备重组人血清白蛋白与干扰素α融合蛋白的方法 |
| CN101503470A (zh) * | 2008-09-27 | 2009-08-12 | 上海交通大学 | rhDSL重组蛋白及其制备方法 |
| JP2010088387A (ja) * | 2008-10-10 | 2010-04-22 | Waseda Univ | 放線菌内で目的タンパク質を誘導発現可能なプロモーター |
-
2013
- 2013-03-14 SG SG11201406112TA patent/SG11201406112TA/en unknown
- 2013-03-14 CN CN201910197577.9A patent/CN109880867A/zh active Pending
- 2013-03-14 RU RU2017139267A patent/RU2017139267A/ru not_active Application Discontinuation
- 2013-03-14 ES ES13713295.7T patent/ES2654424T3/es active Active
- 2013-03-14 AR ARP130100828A patent/AR090340A1/es active IP Right Grant
- 2013-03-14 AU AU2013240280A patent/AU2013240280A1/en not_active Abandoned
- 2013-03-14 KR KR1020147026772A patent/KR102096678B1/ko active Active
- 2013-03-14 CA CA3086730A patent/CA3086730A1/en not_active Abandoned
- 2013-03-14 JP JP2015503300A patent/JP6225162B2/ja active Active
- 2013-03-14 EP EP13713295.7A patent/EP2831261B1/en active Active
- 2013-03-14 BR BR112014023176-1A patent/BR112014023176B1/pt active IP Right Grant
- 2013-03-14 MX MX2014011558A patent/MX365947B/es active IP Right Grant
- 2013-03-14 PL PL13713295T patent/PL2831261T3/pl unknown
- 2013-03-14 SG SG10201508420VA patent/SG10201508420VA/en unknown
- 2013-03-14 SI SI201330894T patent/SI2831261T1/en unknown
- 2013-03-14 SG SG10202007206WA patent/SG10202007206WA/en unknown
- 2013-03-14 CN CN201380027623.1A patent/CN104350156B/zh active Active
- 2013-03-14 MY MYPI2014002775A patent/MY171729A/en unknown
- 2013-03-14 HR HRP20171966TT patent/HRP20171966T1/hr unknown
- 2013-03-14 CA CA2866753A patent/CA2866753C/en active Active
- 2013-03-14 KR KR1020207008828A patent/KR20200037425A/ko not_active Ceased
- 2013-03-14 SG SG10201912877SA patent/SG10201912877SA/en unknown
- 2013-03-14 US US13/826,166 patent/US20140080180A1/en not_active Abandoned
- 2013-03-14 WO PCT/US2013/031383 patent/WO2013148249A1/en not_active Ceased
- 2013-03-14 RU RU2014137554A patent/RU2636353C2/ru active
-
2014
- 2014-08-31 IL IL234377A patent/IL234377B/en active IP Right Grant
- 2014-09-02 ZA ZA2014/06434A patent/ZA201406434B/en unknown
- 2014-09-25 MX MX2018007677A patent/MX2018007677A/es unknown
- 2014-09-25 MX MX2019007032A patent/MX2019007032A/es unknown
- 2014-09-26 US US14/497,964 patent/US9765379B2/en active Active
-
2015
- 2015-06-09 US US14/734,848 patent/US20160130624A1/en not_active Abandoned
-
2017
- 2017-04-13 AU AU2017202484A patent/AU2017202484B2/en active Active
- 2017-10-05 JP JP2017195016A patent/JP2018046825A/ja active Pending
-
2018
- 2018-02-28 US US15/908,526 patent/US20180291411A1/en not_active Abandoned
-
2019
- 2019-02-07 US US16/270,494 patent/US20200199639A1/en not_active Abandoned
- 2019-03-18 JP JP2019049717A patent/JP2019141049A/ja not_active Withdrawn
- 2019-09-05 AU AU2019226192A patent/AU2019226192A1/en not_active Abandoned
- 2019-10-09 AR ARP190102873A patent/AR116615A2/es unknown
-
2020
- 2020-08-14 JP JP2020137053A patent/JP2020202849A/ja not_active Withdrawn
- 2020-10-09 US US17/067,059 patent/US20210171997A1/en not_active Abandoned
-
2022
- 2022-07-13 US US17/864,255 patent/US20230069966A1/en not_active Abandoned
-
2024
- 2024-01-22 US US18/419,368 patent/US20240158825A1/en not_active Abandoned
- 2024-12-20 US US18/990,600 patent/US20250137027A1/en active Pending
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SI2831261T1 (en) | A process for the production of low-level recombinant proteins DHNA (1,4-dihydroxy-2-naphthoate) | |
| De Luca et al. | Downstream processing of therapeutic peptides by means of preparative liquid chromatography | |
| Read et al. | Process analytical technology (PAT) for biopharmaceutical products: Part I. Concepts and applications | |
| McCarthy et al. | Carbamylation of Proteins in 2-D Electrophoresis Myth or Reality? | |
| Low et al. | Separation of human erythrocyte membrane associated proteins with one‐dimensional and two‐dimensional gel electrophoresis followed by identification with matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry | |
| Jiang et al. | Reutilization of food waste: One-step extration, purification and characterization of ovalbumin from salted egg white by aqueous two-phase flotation | |
| Humer et al. | Wanted: more monitoring and control during inclusion body processing | |
| JP2015512259A5 (enExample) | ||
| Lavoie et al. | Targeted capture of Chinese hamster ovary host cell proteins: Peptide ligand discovery | |
| Wei et al. | In vitro production and identification of angiotensin converting enzyme (ACE) inhibitory peptides derived from distilled spent grain prolamin isolate | |
| CN101043899A (zh) | 重折叠转化型生长因子beta家族蛋白 | |
| Jiang et al. | Eco-innovation in reusing food by-products: Separation of ovalbumin from salted egg white using aqueous two-phase system of PEG 1000/(NH4) 2SO4 | |
| Andrade et al. | An integrated approach to aggregate control for therapeutic bispecific antibodies using an improved three column mab platform‐like purification process | |
| Mielczarek et al. | Hemorphins—From discovery to functions and pharmacology | |
| Liu et al. | Challenges and strategies for a thorough characterization of antibody acidic charge variants | |
| Song et al. | Absence of Dx2 at Glu-D1 locus weakens gluten quality potentially regulated by expression of nitrogen metabolism enzymes and glutenin-related genes in wheat | |
| Ribeiro et al. | Proteogenomic characterization of novel x-type high molecular weight glutenin subunit 1Ax1. 1 | |
| ATE474922T1 (de) | Fluoreszenzproteine aus copepoda-spezies und verfahren zur verwendung davon | |
| Fitriani et al. | Impact of gastrointestinal digestion simulation on the formation of Angiotensin-I-Converting Enzyme Inhibitory (ACE-I) peptides from germinated lamtoro gung flour | |
| Matsumoto et al. | Chemical characterization of the water-soluble organic nitrogen in the maritime aerosol | |
| ATE509946T1 (de) | Fluoreszenzproteine und chromoproteine aus nicht- aequorea-hydrozoa-spezies sowie verfahren zur verwendung davon | |
| Dempsey et al. | Solution NMR structure and X-ray absorption analysis of the C-terminal zinc-binding domain of the SecA ATPase | |
| van Schaick et al. | Native liquid chromatography and mass spectrometry to structurally and functionally characterize endo-xylanase proteoforms | |
| Paradela et al. | Evaluation of isotope-coded protein labeling (ICPL) in the quantitative analysis of complex proteomes | |
| Pan et al. | Engineering batch and pulse refolding with transition of aggregation kinetics: an investigation using green fluorescent protein (GFP) |