JP2017513479A5 - - Google Patents

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JP2017513479A5
JP2017513479A5 JP2016562588A JP2016562588A JP2017513479A5 JP 2017513479 A5 JP2017513479 A5 JP 2017513479A5 JP 2016562588 A JP2016562588 A JP 2016562588A JP 2016562588 A JP2016562588 A JP 2016562588A JP 2017513479 A5 JP2017513479 A5 JP 2017513479A5
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transposase
mutation
mutant
seq
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JP2016562588A 2014-04-15 2015-04-15 改善された挿入配列バイアスおよび拡大されたdnaインプット許容差のための修正トランスポザーゼ Active JP6490710B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201461979871P 2014-04-15 2014-04-15
US61/979,871 2014-04-15
US201462062006P 2014-10-09 2014-10-09
US62/062,006 2014-10-09
US201462080882P 2014-11-17 2014-11-17
US62/080,882 2014-11-17
PCT/US2015/025889 WO2015160895A2 (en) 2014-04-15 2015-04-15 Modified transposases for improved insertion sequence bias and increased dna input tolerance

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JP2017513479A JP2017513479A (ja) 2017-06-01
JP2017513479A5 true JP2017513479A5 (enExample) 2018-05-31
JP6490710B2 JP6490710B2 (ja) 2019-03-27

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US (4) US9790476B2 (enExample)
EP (2) EP3132029A2 (enExample)
JP (1) JP6490710B2 (enExample)
CN (3) CN120098956A (enExample)
AU (3) AU2015247779B2 (enExample)
CA (1) CA2946046A1 (enExample)
SG (1) SG11201608585RA (enExample)
TW (1) TWI682997B (enExample)
WO (1) WO2015160895A2 (enExample)

Families Citing this family (133)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
EP2885418A4 (en) 2012-08-14 2016-03-02 10X Genomics Inc MICROCAPSE COMPOSITIONS AND METHOD THEREFOR
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
CN108753766A (zh) 2013-02-08 2018-11-06 10X基因组学有限公司 多核苷酸条形码生成
CN111500680B (zh) 2013-06-25 2024-04-16 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
KR102596508B1 (ko) 2014-04-10 2023-10-30 10엑스 제노믹스, 인크. 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용
TWI682997B (zh) 2014-04-15 2020-01-21 美商伊路米納有限公司 用於改善插入序列傾向及增加dna輸入耐受度之經修飾的轉位酶
CN106715693A (zh) 2014-06-13 2017-05-24 伊卢米纳剑桥有限公司 用于制备序列文库的方法和组合物
CA2953469A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
EP4574974A3 (en) 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CN112430641A (zh) 2014-06-30 2021-03-02 亿明达股份有限公司 使用单侧转座的方法和组合物
KR20170073667A (ko) 2014-10-29 2017-06-28 10엑스 제노믹스, 인크. 표적화 핵산 서열 분석을 위한 방법 및 조성물
EP3215618B1 (en) * 2014-11-05 2019-07-24 Illumina, Inc. Transposase compositions for reduction of insertion bias
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
US10221436B2 (en) 2015-01-12 2019-03-05 10X Genomics, Inc. Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same
EP3259355B1 (en) 2015-02-20 2020-11-25 The Regents of the University of California Methods related to dna sequencing
MX2017010857A (es) 2015-02-24 2017-12-11 10X Genomics Inc Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana.
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
EP4321627A3 (en) 2015-04-10 2024-04-17 10x Genomics Sweden AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
EP3303584B1 (en) 2015-05-29 2019-10-09 Epicentre Technologies Corporation Methods of analyzing nucleic acids
SG10202108763UA (en) 2015-12-04 2021-09-29 10X Genomics Inc Methods and compositions for nucleic acid analysis
EP3426774A4 (en) * 2016-03-10 2019-08-14 The Board of Trustees of the Leland Stanford Junior University TRANSPOSASE-MEDIATED ILLUSTRATION OF ACCESSIBLE GENOMS
JP7022699B2 (ja) * 2016-04-19 2022-02-18 カパ バイオシステムズ, インコーポレイテッド トランスポザーゼ競合物制御系
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
CN106222164B (zh) * 2016-07-28 2020-04-21 元码基因科技(北京)股份有限公司 利用转座子酶进行体外核酸单向扩增的方法、组合物和试剂盒
WO2018027048A1 (en) 2016-08-05 2018-02-08 Bio-Rad Laboratories, Inc. Second strand direct
US11434483B2 (en) * 2016-12-07 2022-09-06 Mgi Tech Co., Ltd. Method for constructing single cell sequencing library and use thereof
CN118441026A (zh) 2016-12-19 2024-08-06 生物辐射实验室股份有限公司 液滴加标的相邻性保留的标签化dna
CN106754811B (zh) * 2016-12-21 2019-05-14 南京诺唯赞生物科技有限公司 一种突变型Tn5转座酶及其制备方法和应用
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN106801044B (zh) * 2017-01-09 2021-03-23 北京全式金生物技术有限公司 一种环状转座子复合物及其应用
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4628582A2 (en) 2017-01-30 2025-10-08 10x Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10920219B2 (en) 2017-02-21 2021-02-16 Illumina, Inc. Tagmentation using immobilized transposomes with linkers
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3445876B1 (en) 2017-05-26 2023-07-05 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN111094587B (zh) * 2017-08-30 2024-08-06 卡帕生物系统公司 转座酶组合物、制备方法和筛选方法
US11733248B2 (en) 2017-09-25 2023-08-22 Fred Hutchinson Cancer Center High efficiency targeted in situ genome-wide profiling
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
EP3704247B1 (en) 2017-11-02 2023-01-04 Bio-Rad Laboratories, Inc. Transposase-based genomic analysis
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US12377396B2 (en) 2018-02-05 2025-08-05 The Board Of Trustees Of The Leland Stanford Junior University Systems and methods for multiplexed measurements in single and ensemble cells
CN108285494B (zh) * 2018-02-11 2021-09-07 北京大学 一种融合蛋白、试剂盒以及CHIP-seq检测方法
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
WO2019184044A1 (zh) * 2018-03-27 2019-10-03 上海欣百诺生物科技有限公司 一种转座酶-抗体结合蛋白的融合蛋白及其制备与应用
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
CN108913712B (zh) * 2018-07-17 2021-04-02 厦门生命互联科技有限公司 一种重组Tn5转座酶的表达纯化方法
US12163179B2 (en) 2018-08-03 2024-12-10 10X Gemomics, Inc. Methods and systems to minimize barcode exchange
US11479816B2 (en) 2018-08-20 2022-10-25 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US12104281B2 (en) 2018-11-30 2024-10-01 Illumina, Inc. Analysis of multiple analytes using a single assay
US20220033888A1 (en) 2018-12-10 2022-02-03 10X Genomics, Inc. Generating capture probes for spatial analysis
MX2021003769A (es) 2018-12-17 2021-05-27 Illumina Inc Sistemas y metodos para controlar reacciones enzimaticas.
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
CN112930405B (zh) 2019-01-11 2025-01-28 Illumina剑桥有限公司 复合表面结合的转座体复合物
US11584953B2 (en) 2019-02-12 2023-02-21 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020223259A1 (en) 2019-04-29 2020-11-05 Illumina, Inc. Identification and analysis of microbial samples by rapid incubation and nucleic acid enrichment
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
KR20220031539A (ko) 2019-07-12 2022-03-11 일루미나 케임브리지 리미티드 전기영동을 이용한 핵산 라이브러리 제조
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
CN110938610B (zh) * 2019-12-16 2020-12-01 广东菲鹏生物有限公司 一种转座酶突变体、融合蛋白、其制备方法和应用
ES2946357T3 (es) 2019-12-23 2023-07-17 10X Genomics Inc Métodos para el análisis espacial usando ligación con molde de ARN
WO2021133842A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
ES2981265T3 (es) 2020-06-08 2024-10-08 10X Genomics Inc Métodos para determinar un margen quirúrgico y métodos de uso del mismo
EP4450639B1 (en) 2020-06-25 2025-10-15 10X Genomics, Inc. Spatial analysis of dna methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
AU2021320307A1 (en) 2020-08-06 2023-02-16 Illumina Cambridge Limited Preparation of RNA and DNA sequencing libraries using bead-linked transposomes
KR20230051508A (ko) 2020-08-18 2023-04-18 일루미나, 인코포레이티드 핵산의 서열-특이적 표적화된 전위 및 선택과 분류
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
CN112164419A (zh) * 2020-09-04 2021-01-01 云舟生物科技(广州)有限公司 在orf中插入蛋白标签的方法以及计算机存储介质
EP4211238A4 (en) * 2020-09-11 2024-09-18 Fred Hutchinson Cancer Center IMPROVED HIGHLY EFFICIENT TARGETED GENOME-WIDE IN-SITU PROFILING
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
KR20230091116A (ko) 2020-10-21 2023-06-22 일루미나, 인코포레이티드 다수의 삽입체를 포함하는 시퀀싱 주형, 및 시퀀싱 처리량을 개선하기 위한 조성물 및 방법
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022164615A1 (en) 2021-01-29 2022-08-04 10X Genomics, Inc. Method for transposase mediated spatial tagging and analyzing genomic dna in a biological sample
EP4288534A1 (en) * 2021-02-05 2023-12-13 Ospedale San Raffaele S.r.l. Engineered transposase and uses thereof
CN112795563A (zh) * 2021-03-23 2021-05-14 上海欣百诺生物科技有限公司 生物素化的转座体在回收CUT&Tag或ATAC-seq产物中的用途及方法
MX2023010814A (es) 2021-03-29 2023-09-27 Illumina Inc Metodos mejorados de preparacion de genotecas.
IL307172A (en) 2021-03-30 2023-11-01 Illumina Inc Improved methods of isothermal complementary DNA and library preparation
MX2023011218A (es) 2021-03-31 2023-10-02 Illumina Inc Métodos de preparación de genotecas de secuenciación de tagmentación direccional usando tecnología basada en transposón con identificadores moleculares únicos para la corrección de errores.
EP4428246A3 (en) 2021-04-14 2024-11-27 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
WO2022233821A1 (en) * 2021-05-03 2022-11-10 European Molecular Biology Laboratory Methods for isolating and/or obtaining captured polynucleotide fragments
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
EP4347879B1 (en) 2021-06-03 2025-02-19 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
CN114807084B (zh) * 2022-04-26 2023-05-16 翌圣生物科技(上海)股份有限公司 突变型Tn5转座酶及试剂盒
WO2023225519A1 (en) 2022-05-17 2023-11-23 10X Genomics, Inc. Modified transposons, compositions and uses thereof
CN115927244A (zh) * 2022-05-26 2023-04-07 上海墨卓生物科技有限公司 一种基于微流控技术的单细胞ChIP-seq文库的构建方法
CN115948363B (zh) * 2022-08-26 2024-02-27 武汉影子基因科技有限公司 Tn5转座酶突变体及其制备方法和应用
CN115785283B (zh) * 2022-11-02 2024-05-31 武汉影子基因科技有限公司 PAG-Tn5突变体及其应用
WO2024137934A1 (en) * 2022-12-21 2024-06-27 The Trustees Of Columbia University In The City Of New York Use of pa-tn5 to generate dna libraries
US20250207193A1 (en) 2023-12-22 2025-06-26 Illumina, Inc. Reusable flow cells and methods of using them for nucleic acid sequencing
WO2025244841A1 (en) 2024-05-23 2025-11-27 Illumina, Inc. Methods and systems for quantifying targeted enrichment efficiency

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US5925545A (en) 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US6159736A (en) * 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
EP1198583A1 (en) * 1999-08-02 2002-04-24 Wisconsin Alumni Research Foundation Mutant tn5 transposase enzymes and method for their use
US7262056B2 (en) * 2001-11-08 2007-08-28 Mirus Bio Corporation Enhancing intermolecular integration of nucleic acids using integrator complexes
US7083980B2 (en) 2003-04-17 2006-08-01 Wisconsin Alumni Research Foundation Tn5 transposase mutants and the use thereof
US7608434B2 (en) * 2004-08-04 2009-10-27 Wisconsin Alumni Research Foundation Mutated Tn5 transposase proteins and the use thereof
KR100890187B1 (ko) 2007-09-06 2009-03-25 고려대학교 산학협력단 Tsf를 융합파트너로 이용한 재조합 단백질의 제조방법
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
HUE027961T2 (en) * 2008-10-24 2016-11-28 Epicentre Tech Corp Transposon final preparations and methods for modifying nucleic acids
US9005935B2 (en) 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
WO2013085918A1 (en) 2011-12-05 2013-06-13 The Regents Of The University Of California Methods and compostions for generating polynucleic acid fragments
TWI682997B (zh) 2014-04-15 2020-01-21 美商伊路米納有限公司 用於改善插入序列傾向及增加dna輸入耐受度之經修飾的轉位酶

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