SG11201608585RA - Modified transposases for improved insertion sequence bias and increased dna input tolerance - Google Patents

Modified transposases for improved insertion sequence bias and increased dna input tolerance

Info

Publication number
SG11201608585RA
SG11201608585RA SG11201608585RA SG11201608585RA SG11201608585RA SG 11201608585R A SG11201608585R A SG 11201608585RA SG 11201608585R A SG11201608585R A SG 11201608585RA SG 11201608585R A SG11201608585R A SG 11201608585RA SG 11201608585R A SG11201608585R A SG 11201608585RA
Authority
SG
Singapore
Prior art keywords
insertion sequence
increased dna
dna input
improved insertion
sequence bias
Prior art date
Application number
SG11201608585RA
Inventor
Christian Gloeckner
Amirali Kia
Erin Bomati
Molly He
Haiying Li Grunenwald
Scott Kuersten
Trina Faye Osothprarop
Darin Haskins
Joshua Burgess
Anupama Khanna
Daniel Schlingman
Ramesh Vaidyanathan
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of SG11201608585RA publication Critical patent/SG11201608585RA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11201608585RA 2014-04-15 2015-04-15 Modified transposases for improved insertion sequence bias and increased dna input tolerance SG11201608585RA (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201461979871P 2014-04-15 2014-04-15
US201462062006P 2014-10-09 2014-10-09
US201462080882P 2014-11-17 2014-11-17
PCT/US2015/025889 WO2015160895A2 (en) 2014-04-15 2015-04-15 Modified transposases for improved insertion sequence bias and increased dna input tolerance

Publications (1)

Publication Number Publication Date
SG11201608585RA true SG11201608585RA (en) 2016-11-29

Family

ID=53008897

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201608585RA SG11201608585RA (en) 2014-04-15 2015-04-15 Modified transposases for improved insertion sequence bias and increased dna input tolerance

Country Status (9)

Country Link
US (4) US9790476B2 (en)
EP (2) EP3845640A3 (en)
JP (1) JP6490710B2 (en)
CN (2) CN106507677B (en)
AU (2) AU2015247779B2 (en)
CA (1) CA2946046A1 (en)
SG (1) SG11201608585RA (en)
TW (1) TWI682997B (en)
WO (1) WO2015160895A2 (en)

Families Citing this family (105)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140155295A1 (en) 2012-08-14 2014-06-05 10X Technologies, Inc. Capsule array devices and methods of use
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014124338A1 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation
CN118240918A (en) 2013-06-25 2024-06-25 普罗格诺西斯生物科学公司 Spatially encoded bioanalytical analysis using microfluidic devices
WO2015157567A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
WO2015160895A2 (en) 2014-04-15 2015-10-22 Illumina, Inc. Modified transposases for improved insertion sequence bias and increased dna input tolerance
CA2952058A1 (en) 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
MX2016016902A (en) 2014-06-26 2017-03-27 10X Genomics Inc Methods of analyzing nucleic acids from individual cells or cell populations.
SG11201610910QA (en) 2014-06-30 2017-01-27 Illumina Inc Methods and compositions using one-sided transposition
CN114807307A (en) 2014-10-29 2022-07-29 10X 基因组学有限公司 Methods and compositions for sequencing target nucleic acids
ES2947757T3 (en) * 2014-11-05 2023-08-18 Illumina Inc Transposase compositions to reduce insertion bias
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
CN107427808B (en) 2015-01-12 2020-10-23 10X基因组学有限公司 Method and system for preparing nucleic acid sequencing library and library prepared by using same
WO2016134218A1 (en) 2015-02-20 2016-08-25 The Regents Of The University Of California Methods related to dna sequencing
WO2016138148A1 (en) 2015-02-24 2016-09-01 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
EP4119677B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US10640809B2 (en) 2015-05-29 2020-05-05 Epicentre Technologies Corporation Methods of analyzing nucleic acids
ES2926495T3 (en) 2015-12-04 2022-10-26 10X Genomics Inc Methods and compositions for nucleic acid analysis
SG11201807444PA (en) * 2016-03-10 2018-09-27 Univ Leland Stanford Junior Transposase-mediated imaging of the accessible genome
EP4194563A1 (en) * 2016-04-19 2023-06-14 Kapa Biosystems, Inc. Transposase competitor control system
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
CN106222164B (en) * 2016-07-28 2020-04-21 元码基因科技（北京）股份有限公司 Methods, compositions and kits for unidirectional amplification of nucleic acids in vitro using transposase
WO2018027048A1 (en) 2016-08-05 2018-02-08 Bio-Rad Laboratories, Inc. Second strand direct
EP3553180B1 (en) * 2016-12-07 2022-05-04 MGI Tech Co., Ltd. Method for constructing single cell sequencing library and use thereof
CN110139932B (en) 2016-12-19 2024-05-17 生物辐射实验室股份有限公司 Drop-on labeled DNA with maintained adjacency
CN106754811B (en) * 2016-12-21 2019-05-14 南京诺唯赞生物科技有限公司 A kind of saltant type Tn5 transposase and its preparation method and application
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN106801044B (en) * 2017-01-09 2021-03-23 北京全式金生物技术有限公司 Cyclic transposon complex and application thereof
CN117512066A (en) 2017-01-30 2024-02-06 10X基因组学有限公司 Method and system for droplet-based single cell bar coding
IL263118B2 (en) 2017-02-21 2023-12-01 Illumina Inc Tagmentation using immobilized transposomes with linkers
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN109526228B (en) 2017-05-26 2022-11-25 10X基因组学有限公司 Single cell analysis of transposase accessible chromatin
CN111094587A (en) * 2017-08-30 2020-05-01 卡帕生物系统公司 Transposase compositions, methods of preparation and screening methods
JP7241069B2 (en) 2017-09-25 2023-03-16 フレッド ハッチンソン キャンサー センター Highly efficient targeted in situ genome-wide profiling
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods and systems for nuclecic acid preparation and chromatin analysis
CN118186055A (en) 2017-11-02 2024-06-14 生物辐射实验室股份有限公司 Transposase-based genomic analysis
WO2019099751A1 (en) 2017-11-15 2019-05-23 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CN108285494B (en) * 2018-02-11 2021-09-07 北京大学 Fusion protein, kit and CHIP-seq detection method
EP3752832A1 (en) 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
WO2019184044A1 (en) * 2018-03-27 2019-10-03 上海欣百诺生物科技有限公司 Fusion protein of transposase-antibody binding protein and preparation and application thereof
EP3775271A1 (en) 2018-04-06 2021-02-17 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
CN108913712B (en) * 2018-07-17 2021-04-02 厦门生命互联科技有限公司 Expression and purification method of recombinant Tn5 transposase
CN113166807B (en) 2018-08-20 2024-06-04 生物辐射实验室股份有限公司 Nucleotide sequence generation by co-localization of barcode beads in partitions
EP4293126A3 (en) 2018-11-30 2024-01-17 Illumina, Inc. Analysis of multiple analytes using a single assay
US20220064630A1 (en) 2018-12-10 2022-03-03 10X Genomics, Inc. Resolving spatial arrays using deconvolution
CN112867800B (en) 2018-12-17 2024-07-12 Illumina公司 Methods and means for preparing sequencing libraries
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
BR112021006038A2 (en) 2019-01-11 2021-10-26 Illumina Cambridge Limited STRAPOSOME COMPLEXES CONNECTED TO THE SURFACE OF THE COMPLEX
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
EP3963087A1 (en) 2019-04-29 2022-03-09 Illumina Inc Identification and analysis of microbial samples by rapid incubation and nucleic acid enrichment
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
AU2020312787A1 (en) 2019-07-12 2021-06-17 Illumina Cambridge Limited Nucleic acid library preparation using electrophoresis
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
CN110938610B (en) * 2019-12-16 2020-12-01 广东菲鹏生物有限公司 Transposase mutant, fusion protein, preparation method and application thereof
ES2946357T3 (en) 2019-12-23 2023-07-17 10X Genomics Inc Methods for spatial analysis using RNA template ligation
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
ES2965354T3 (en) 2020-04-22 2024-04-12 10X Genomics Inc Methods for spatial analysis using targeted RNA deletion
EP4153775A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
KR20230041725A (en) 2020-08-06 2023-03-24 일루미나, 인코포레이티드 Construction of RNA and DNA sequencing libraries using bead-linked transposomes
KR20230051508A (en) 2020-08-18 2023-04-18 일루미나, 인코포레이티드 Sequence-specific targeted translocation and selection and sorting of nucleic acids
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
CN112164419A (en) * 2020-09-04 2021-01-01 云舟生物科技（广州）有限公司 Method for inserting protein tag into ORF and computer storage medium
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
IL302207A (en) 2020-10-21 2023-06-01 Illumina Inc Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20240093290A1 (en) 2021-01-29 2024-03-21 10X Genomics, Inc. Method for transposase mediated spatial tagging and analyzing genomic dna in a biological sample
WO2022167665A1 (en) * 2021-02-05 2022-08-11 Ospedale San Raffaele S.R.L. Engineered transposase and uses thereof
CN112795563A (en) * 2021-03-23 2021-05-14 上海欣百诺生物科技有限公司 Use and method of biotinylated transposomes for recovering CUT & Tag or ATAC-seq products
EP4314279A1 (en) 2021-03-29 2024-02-07 Illumina, Inc. Improved methods of library preparation
KR20230161955A (en) 2021-03-30 2023-11-28 일루미나, 인코포레이티드 Improved methods for isothermal complementary DNA and library preparation
JP2024511760A (en) 2021-03-31 2024-03-15 イルミナ インコーポレイテッド Method for preparing directional tagmentation sequencing libraries using transposon-based technology with unique molecular identifiers for error correction
EP4334442A1 (en) * 2021-05-03 2024-03-13 European Molecular Biology Laboratory Methods for isolating and/or obtaining captured polynucleotide fragments
EP4196605A1 (en) 2021-09-01 2023-06-21 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
CN114807084B (en) * 2022-04-26 2023-05-16 翌圣生物科技(上海)股份有限公司 Mutant Tn5 transposase and kit
WO2023225519A1 (en) 2022-05-17 2023-11-23 10X Genomics, Inc. Modified transposons, compositions and uses thereof
CN115948363B (en) * 2022-08-26 2024-02-27 武汉影子基因科技有限公司 Tn5 transposase mutant and preparation method and application thereof
CN115785283B (en) * 2022-11-02 2024-05-31 武汉影子基因科技有限公司 PAG-Tn5 mutant and application thereof

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US5925545A (en) 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US6159736A (en) * 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
EP1198583A1 (en) * 1999-08-02 2002-04-24 Wisconsin Alumni Research Foundation Mutant tn5 transposase enzymes and method for their use
US7262056B2 (en) * 2001-11-08 2007-08-28 Mirus Bio Corporation Enhancing intermolecular integration of nucleic acids using integrator complexes
US7083980B2 (en) * 2003-04-17 2006-08-01 Wisconsin Alumni Research Foundation Tn5 transposase mutants and the use thereof
US7608434B2 (en) * 2004-08-04 2009-10-27 Wisconsin Alumni Research Foundation Mutated Tn5 transposase proteins and the use thereof
KR100890187B1 (en) 2007-09-06 2009-03-25 고려대학교 산학협력단 PREPARATION METHOD OF RECOMBINANT PROTEIN BY USE OF Tsf AS A FUSION EXPRESSION PARTNER
EP2787565B1 (en) * 2008-10-24 2015-09-16 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9005935B2 (en) 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
WO2013085918A1 (en) 2011-12-05 2013-06-13 The Regents Of The University Of California Methods and compostions for generating polynucleic acid fragments
WO2015160895A2 (en) 2014-04-15 2015-10-22 Illumina, Inc. Modified transposases for improved insertion sequence bias and increased dna input tolerance

Also Published As

Publication number Publication date
AU2021236488B2 (en) 2024-06-20
EP3132029A2 (en) 2017-02-22
US10035992B2 (en) 2018-07-31
CA2946046A1 (en) 2015-10-22
AU2015247779A1 (en) 2016-11-03
AU2015247779B2 (en) 2021-06-24
WO2015160895A2 (en) 2015-10-22
CN106507677A (en) 2017-03-15
JP6490710B2 (en) 2019-03-27
US9790476B2 (en) 2017-10-17
TW201621048A (en) 2016-06-16
US10385323B2 (en) 2019-08-20
TWI682997B (en) 2020-01-21
AU2021236488A1 (en) 2021-10-21
CN113005108A (en) 2021-06-22
JP2017513479A (en) 2017-06-01
US20150291942A1 (en) 2015-10-15
US20190359955A1 (en) 2019-11-28
US10544403B2 (en) 2020-01-28
WO2015160895A3 (en) 2016-01-14
EP3845640A2 (en) 2021-07-07
US20180298356A1 (en) 2018-10-18
CN106507677B (en) 2021-03-12
EP3845640A3 (en) 2021-09-01
US20180171311A1 (en) 2018-06-21

Similar Documents

Publication Publication Date Title
SG11201608585RA (en) Modified transposases for improved insertion sequence bias and increased dna input tolerance
IL280941A (en) Modified double-stranded rna agents
HK1257676A1 (en) Engineered crispr-cas9 nucleases
IL286785A (en) Enzymes and applications thereof
IL249461A0 (en) Processes and systems for nucleic acid sequence assembly
EP3157573A4 (en) Alternative nucleic acid molecules and uses thereof
EP3157572A4 (en) Alternative nucleic acid molecules and uses thereof
PL3094731T3 (en) Saccharide-modified nucleic acid molecules
GB201408841D0 (en) Nucleic acid processing
EP3324275A4 (en) Operation-sensation imparting input device
HK1250682A1 (en) Nucleic acid amplification
SG11201707256VA (en) Treatment-instrument insertion aid
IL257500A (en) Modified cullin1 gene
GB201517241D0 (en) DNA modification
GB2538064B (en) Improved coupling and method thereof
PL3180295T3 (en) Process for fast humification and biostabilization
SG11201707305PA (en) Treatment-instrument insertion aid
GB201409786D0 (en) Nucleic acid amplification system
GB2538193B (en) Alphabet input system
GB2554258B (en) Improved input device
GB201409709D0 (en) Improved deoxyribonuclease enzymes
GB201509037D0 (en) Novel conjugation process and reagents
GB201520696D0 (en) Pen shafts
GB201418989D0 (en) Novel conjugation process and reagents
GB201418186D0 (en) Novel conjugation process and reagents