CN120098956A - 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶 - Google Patents

用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶 Download PDF

Info

Publication number
CN120098956A
CN120098956A CN202510139371.6A CN202510139371A CN120098956A CN 120098956 A CN120098956 A CN 120098956A CN 202510139371 A CN202510139371 A CN 202510139371A CN 120098956 A CN120098956 A CN 120098956A
Authority
CN
China
Prior art keywords
transposase
dna
library
tag
tagged
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202510139371.6A
Other languages
English (en)
Chinese (zh)
Inventor
克里斯蒂安·格勒克纳
阿米尔阿里·基亚
艾琳·博马蒂
莫利·何
海缨·李·格鲁内瓦德
斯科特·库尔斯腾
特瑞纳·法耶·奥索斯普拉罗普
达林·哈斯金斯
约书亚·伯吉斯
阿奴巴玛·康纳
丹尼尔·施凌曼
拉梅什·威德亚纳森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of CN120098956A publication Critical patent/CN120098956A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202510139371.6A 2014-04-15 2015-04-15 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶 Pending CN120098956A (zh)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201461979871P 2014-04-15 2014-04-15
US61/979,871 2014-04-15
US201462062006P 2014-10-09 2014-10-09
US62/062,006 2014-10-09
US201462080882P 2014-11-17 2014-11-17
US62/080,882 2014-11-17
PCT/US2015/025889 WO2015160895A2 (en) 2014-04-15 2015-04-15 Modified transposases for improved insertion sequence bias and increased dna input tolerance
CN201580027103.XA CN106507677B (zh) 2014-04-15 2015-04-15 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201580027103.XA Division CN106507677B (zh) 2014-04-15 2015-04-15 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶

Publications (1)

Publication Number Publication Date
CN120098956A true CN120098956A (zh) 2025-06-06

Family

ID=53008897

Family Applications (3)

Application Number Title Priority Date Filing Date
CN201580027103.XA Active CN106507677B (zh) 2014-04-15 2015-04-15 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶
CN202110295229.2A Active CN113005108B (zh) 2014-04-15 2015-04-15 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶
CN202510139371.6A Pending CN120098956A (zh) 2014-04-15 2015-04-15 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶

Family Applications Before (2)

Application Number Title Priority Date Filing Date
CN201580027103.XA Active CN106507677B (zh) 2014-04-15 2015-04-15 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶
CN202110295229.2A Active CN113005108B (zh) 2014-04-15 2015-04-15 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶

Country Status (9)

Country Link
US (4) US9790476B2 (enExample)
EP (2) EP3845640A3 (enExample)
JP (1) JP6490710B2 (enExample)
CN (3) CN106507677B (enExample)
AU (3) AU2015247779B2 (enExample)
CA (1) CA2946046A1 (enExample)
SG (1) SG11201608585RA (enExample)
TW (1) TWI682997B (enExample)
WO (1) WO2015160895A2 (enExample)

Families Citing this family (133)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
BR112015019159A2 (pt) 2013-02-08 2017-07-18 10X Genomics Inc geração de código de barras de polinucleotídeos
EP4234716A3 (en) 2013-06-25 2023-12-06 Prognosys Biosciences, Inc. Methods for determining spatial patterns of biological targets in a sample
CN106413896B (zh) 2014-04-10 2019-07-05 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
EP3845640A3 (en) 2014-04-15 2021-09-01 Illumina, Inc. Modified tranposases for improved insertion sequence bias and increased dna input tolerence
CA3172086A1 (en) 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
JP2017522866A (ja) 2014-06-26 2017-08-17 １０エックス ジェノミクス， インコーポレイテッド 核酸配列の分析
ES2713153T3 (es) 2014-06-30 2019-05-20 Illumina Inc Métodos y composiciones que utilizan la transposición unilateral
US20160122817A1 (en) 2014-10-29 2016-05-05 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
EP4239067A3 (en) * 2014-11-05 2023-10-25 Illumina, Inc. Transposase compositions for reduction of insertion bias
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
US10988805B2 (en) 2015-02-20 2021-04-27 The Regents Of The University Of California Methods related to DNA sequencing
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
CN115651972A (zh) 2015-02-24 2023-01-31 10X 基因组学有限公司 用于靶向核酸序列覆盖的方法
EP4119677B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US10640809B2 (en) 2015-05-29 2020-05-05 Epicentre Technologies Corporation Methods of analyzing nucleic acids
CN115369161A (zh) 2015-12-04 2022-11-22 10X 基因组学有限公司 用于核酸分析的方法和组合物
SG11201807444PA (en) * 2016-03-10 2018-09-27 Univ Leland Stanford Junior Transposase-mediated imaging of the accessible genome
US11932847B2 (en) * 2016-04-19 2024-03-19 Kapa Biosystems, Inc. Transposase competitor control system
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
CN106222164B (zh) * 2016-07-28 2020-04-21 元码基因科技（北京）股份有限公司 利用转座子酶进行体外核酸单向扩增的方法、组合物和试剂盒
CN113151423B (zh) 2016-08-05 2024-09-10 生物辐射实验室股份有限公司 第二链引导
ES2922281T3 (es) * 2016-12-07 2022-09-12 Mgi Tech Co Ltd Método para construir una biblioteca de secuenciación de una célula individual y uso del mismo
CN110139932B (zh) 2016-12-19 2024-05-17 生物辐射实验室股份有限公司 液滴加标的相邻性保留的标签化dna
CN106754811B (zh) * 2016-12-21 2019-05-14 南京诺唯赞生物科技有限公司 一种突变型Tn5转座酶及其制备方法和应用
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN106801044B (zh) * 2017-01-09 2021-03-23 北京全式金生物技术有限公司 一种环状转座子复合物及其应用
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10920219B2 (en) 2017-02-21 2021-02-16 Illumina, Inc. Tagmentation using immobilized transposomes with linkers
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
CN111094587B (zh) * 2017-08-30 2024-08-06 卡帕生物系统公司 转座酶组合物、制备方法和筛选方法
US11733248B2 (en) 2017-09-25 2023-08-22 Fred Hutchinson Cancer Center High efficiency targeted in situ genome-wide profiling
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN118186055A (zh) 2017-11-02 2024-06-14 生物辐射实验室股份有限公司 基于转座酶的基因组分析
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CN112004920B (zh) 2018-02-05 2024-08-02 斯坦福大学托管董事会 用于单细胞和集合细胞的多重测量的系统和方法
CN108285494B (zh) * 2018-02-11 2021-09-07 北京大学 一种融合蛋白、试剂盒以及CHIP-seq检测方法
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
WO2019184044A1 (zh) * 2018-03-27 2019-10-03 上海欣百诺生物科技有限公司 一种转座酶-抗体结合蛋白的融合蛋白及其制备与应用
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
CN108913712B (zh) * 2018-07-17 2021-04-02 厦门生命互联科技有限公司 一种重组Tn5转座酶的表达纯化方法
CN112703252B (zh) 2018-08-03 2024-09-10 10X基因组学有限公司 用于最小化条形码交换的方法和系统
US11479816B2 (en) 2018-08-20 2022-10-25 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
BR112021006183A2 (pt) 2018-11-30 2021-06-29 Illumina, Inc. análise de múltiplos analitos com o uso de um único ensaio
US12385083B2 (en) 2018-12-10 2025-08-12 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
EP3899028A1 (en) 2018-12-17 2021-10-27 Illumina, Inc. Methods and means for preparing a library for sequencing
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
KR20210114918A (ko) 2019-01-11 2021-09-24 일루미나 케임브리지 리미티드 복합체 표면-결합 트랜스포좀 복합체
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
EP3924505B1 (en) 2019-02-12 2025-12-17 10X Genomics, Inc. Methods for processing nucleic acid molecules
BR112021006173A2 (pt) 2019-04-29 2021-11-16 Illumina Inc Métodos para a identificação e análise de micro-organismos viáveis e/ou proliferativos e para determinação da eficácia de um agente antimicrobiano na modulação do crescimento e da proliferação de micro-organismos em uma amostra
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
JP7603586B2 (ja) 2019-07-12 2024-12-20 イルミナ ケンブリッジ リミテッド 電気泳動を用いた核酸ライブラリの調製
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
CN110938610B (zh) * 2019-12-16 2020-12-01 广东菲鹏生物有限公司 一种转座酶突变体、融合蛋白、其制备方法和应用
WO2021133849A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
WO2021133842A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
EP4549582A3 (en) 2020-04-22 2025-06-18 10x Genomics, Inc. Methods for spatial analysis using targeted rna depletion
EP4553168A3 (en) 2020-05-22 2025-08-27 10x Genomics, Inc. Spatial analysis to detect sequence variants
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
ES2981265T3 (es) 2020-06-08 2024-10-08 10X Genomics Inc Métodos para determinar un margen quirúrgico y métodos de uso del mismo
EP4450639B1 (en) 2020-06-25 2025-10-15 10X Genomics, Inc. Spatial analysis of dna methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
WO2022031955A1 (en) 2020-08-06 2022-02-10 Illumina, Inc. Preparation of rna and dna sequencing libraries using bead-linked transposomes
CN116323971A (zh) 2020-08-18 2023-06-23 Illumina公司 核酸的序列特异性靶向转座和选择以及分选
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
CN112164419A (zh) * 2020-09-04 2021-01-01 云舟生物科技（广州）有限公司 在orf中插入蛋白标签的方法以及计算机存储介质
CA3191834A1 (en) * 2020-09-11 2022-03-17 Steven Henikoff Improved high efficiency targeted in situ genome-wide profiling
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
AU2021366658A1 (en) 2020-10-21 2023-06-22 Illumina Cambridge Limited Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4121555A1 (en) 2020-12-21 2023-01-25 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20240093290A1 (en) 2021-01-29 2024-03-21 10X Genomics, Inc. Method for transposase mediated spatial tagging and analyzing genomic dna in a biological sample
EP4288534A1 (en) * 2021-02-05 2023-12-13 Ospedale San Raffaele S.r.l. Engineered transposase and uses thereof
CN112795563A (zh) * 2021-03-23 2021-05-14 上海欣百诺生物科技有限公司 生物素化的转座体在回收CUT&Tag或ATAC-seq产物中的用途及方法
JP2024511766A (ja) 2021-03-29 2024-03-15 イルミナ インコーポレイテッド 改善されたライブラリ調製方法
MX2023010495A (es) 2021-03-30 2023-09-18 Illumina Inc Metodos mejorados de preparacion de genoteca y adn complementarios isotermicos.
IL307164A (en) 2021-03-31 2023-11-01 Illumina Inc Methods for preparing sequence libraries for directional labeling using transposon-based technology with unique molecular identifiers for error correction
WO2022221425A1 (en) 2021-04-14 2022-10-20 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4334442A1 (en) * 2021-05-03 2024-03-13 European Molecular Biology Laboratory Methods for isolating and/or obtaining captured polynucleotide fragments
WO2022236054A1 (en) 2021-05-06 2022-11-10 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
EP4582555A3 (en) 2021-06-03 2025-10-22 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
EP4196605B1 (en) 2021-09-01 2024-12-04 10X Genomics, Inc. Methods for blocking a capture probe on a spatial array
EP4419707A1 (en) 2021-11-10 2024-08-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
CN114807084B (zh) * 2022-04-26 2023-05-16 翌圣生物科技(上海)股份有限公司 突变型Tn5转座酶及试剂盒
WO2023225519A1 (en) 2022-05-17 2023-11-23 10X Genomics, Inc. Modified transposons, compositions and uses thereof
CN115927244A (zh) * 2022-05-26 2023-04-07 上海墨卓生物科技有限公司 一种基于微流控技术的单细胞ChIP-seq文库的构建方法
CN115948363B (zh) * 2022-08-26 2024-02-27 武汉影子基因科技有限公司 Tn5转座酶突变体及其制备方法和应用
CN115785283B (zh) * 2022-11-02 2024-05-31 武汉影子基因科技有限公司 PAG-Tn5突变体及其应用
WO2024137934A1 (en) * 2022-12-21 2024-06-27 The Trustees Of Columbia University In The City Of New York Use of pa-tn5 to generate dna libraries
WO2025137479A1 (en) 2023-12-22 2025-06-26 Illumina, Inc. Reusable flow cells and methods of using them for nucleic acid sequencing
WO2025244841A1 (en) 2024-05-23 2025-11-27 Illumina, Inc. Methods and systems for quantifying targeted enrichment efficiency

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US5925545A (en) 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US6159736A (en) * 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
JP2003505104A (ja) * 1999-08-02 2003-02-12 ウイスコンシン アラムニ リサーチ ファンデーション 変異体ｔｎ５トランスポザーゼ酵素及びその使用方法
US7262056B2 (en) * 2001-11-08 2007-08-28 Mirus Bio Corporation Enhancing intermolecular integration of nucleic acids using integrator complexes
US7083980B2 (en) 2003-04-17 2006-08-01 Wisconsin Alumni Research Foundation Tn5 transposase mutants and the use thereof
US7608434B2 (en) * 2004-08-04 2009-10-27 Wisconsin Alumni Research Foundation Mutated Tn5 transposase proteins and the use thereof
KR100890187B1 (ko) 2007-09-06 2009-03-25 고려대학교 산학협력단 Ｔｓｆ를 융합파트너로 이용한 재조합 단백질의 제조방법
DK2376517T3 (da) * 2008-10-24 2013-02-11 Epict Technologies Corp Transposon-ende-sammensætninger og fremgangsmåder til at modificere nukleinsyrer
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9005935B2 (en) 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
WO2013085918A1 (en) 2011-12-05 2013-06-13 The Regents Of The University Of California Methods and compostions for generating polynucleic acid fragments
EP3845640A3 (en) 2014-04-15 2021-09-01 Illumina, Inc. Modified tranposases for improved insertion sequence bias and increased dna input tolerence

Also Published As

Publication number Publication date
JP2017513479A (ja) 2017-06-01
CN113005108B (zh) 2025-02-11
US10544403B2 (en) 2020-01-28
WO2015160895A3 (en) 2016-01-14
US20150291942A1 (en) 2015-10-15
US20190359955A1 (en) 2019-11-28
US10035992B2 (en) 2018-07-31
CN106507677B (zh) 2021-03-12
SG11201608585RA (en) 2016-11-29
AU2021236488A1 (en) 2021-10-21
TWI682997B (zh) 2020-01-21
US20180171311A1 (en) 2018-06-21
AU2021236488B2 (en) 2024-06-20
CN106507677A (zh) 2017-03-15
EP3845640A2 (en) 2021-07-07
AU2024205075A1 (en) 2024-10-03
JP6490710B2 (ja) 2019-03-27
CN113005108A (zh) 2021-06-22
EP3845640A3 (en) 2021-09-01
US10385323B2 (en) 2019-08-20
US9790476B2 (en) 2017-10-17
EP3132029A2 (en) 2017-02-22
AU2015247779A1 (en) 2016-11-03
AU2015247779B2 (en) 2021-06-24
TW201621048A (zh) 2016-06-16
WO2015160895A2 (en) 2015-10-22
CA2946046A1 (en) 2015-10-22
US20180298356A1 (en) 2018-10-18

Similar Documents

Publication Publication Date Title
CN113005108B (zh) 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶
CN113811608B (zh) 毛螺菌科细菌nd2006 cas12a突变型基因和由其编码的多肽
JP6397612B2 (ja) Ｄｎａ断片化および標的化のための固定化されたトランスポザーゼ複合体
Garinot-Schneider et al. Identification of putative active-site residues in the DNase domain of colicin E9 by random mutagenesis
EP1913149A2 (en) Targeted integration and expression of exogenous nucleic acid sequences
JP6879519B2 (ja) リコンビナーゼ変異体
WO2020068196A2 (en) Proteins that inhibit cas12a (cpf1), a crispr-cas nuclease
CA2837503C (en) Sequence-specific engineered ribonuclease h and the method for determining the sequence preference of dna-rna hybrid binding proteins
HK40055417A (en) Modified transposases for improved insertion sequence bias and increased dna input tolerance
HK40055417B (zh) 用於改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶
Lawrie et al. A high throughput approach for the generation of orthogonally interacting protein pairs
HK40049185A (en) Modified transposases for improved insertion sequence bias and increased dna input tolerance
US6818410B2 (en) Methods of isolating RNA-binding proteins and compositions related to the same
WO2025049563A1 (en) A programmable gene correction technology using cas-polymerase constructs
US20140080738A1 (en) Engineered transposon for facile construction of a random protein domain insertion library
HK1235066B (en) Recombinase mutants
HK1235066A1 (en) Recombinase mutants

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination