CA2946046A1 - Modified transposases for improved insertion sequence bias and increased dna input tolerance - Google Patents

Modified transposases for improved insertion sequence bias and increased dna input tolerance Download PDF

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Publication number
CA2946046A1
CA2946046A1 CA2946046A CA2946046A CA2946046A1 CA 2946046 A1 CA2946046 A1 CA 2946046A1 CA 2946046 A CA2946046 A CA 2946046A CA 2946046 A CA2946046 A CA 2946046A CA 2946046 A1 CA2946046 A1 CA 2946046A1
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transposase
mutation
mutant
dna
substitution
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French (fr)
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Christian Gloeckner
Amirali Kia
Erin BOMATI
Molly He
Haiying Li Grunenwald
Scott Kuersten
Trina Faye Osothprarop
Darin Haskins
Joshua Burgess
Anupama Khanna
Daniel Schlingman
Ramesh Vaidyanathan
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Illumina Inc
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Illumina Inc
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1068Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

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  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
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  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2946046A 2014-04-15 2015-04-15 Modified transposases for improved insertion sequence bias and increased dna input tolerance Pending CA2946046A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201461979871P 2014-04-15 2014-04-15
US61/979,871 2014-04-15
US201462062006P 2014-10-09 2014-10-09
US62/062,006 2014-10-09
US201462080882P 2014-11-17 2014-11-17
US62/080,882 2014-11-17
PCT/US2015/025889 WO2015160895A2 (en) 2014-04-15 2015-04-15 Modified transposases for improved insertion sequence bias and increased dna input tolerance

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CA2946046A1 true CA2946046A1 (en) 2015-10-22

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US (4) US9790476B2 (enExample)
EP (2) EP3845640A3 (enExample)
JP (1) JP6490710B2 (enExample)
CN (3) CN106507677B (enExample)
AU (3) AU2015247779B2 (enExample)
CA (1) CA2946046A1 (enExample)
SG (1) SG11201608585RA (enExample)
TW (1) TWI682997B (enExample)
WO (1) WO2015160895A2 (enExample)

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