JP2014513534A5 - - Google Patents
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- Publication number
- JP2014513534A5 JP2014513534A5 JP2014506607A JP2014506607A JP2014513534A5 JP 2014513534 A5 JP2014513534 A5 JP 2014513534A5 JP 2014506607 A JP2014506607 A JP 2014506607A JP 2014506607 A JP2014506607 A JP 2014506607A JP 2014513534 A5 JP2014513534 A5 JP 2014513534A5
- Authority
- JP
- Japan
- Prior art keywords
- temperature
- dna polymerase
- nucleic acid
- template
- target sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000000034 method Methods 0.000 claims 51
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 18
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 18
- 108020004707 nucleic acids Proteins 0.000 claims 17
- 102000039446 nucleic acids Human genes 0.000 claims 17
- 150000007523 nucleic acids Chemical class 0.000 claims 17
- 230000003321 amplification Effects 0.000 claims 10
- 238000003199 nucleic acid amplification method Methods 0.000 claims 10
- 239000011541 reaction mixture Substances 0.000 claims 9
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims 8
- 150000001768 cations Chemical class 0.000 claims 8
- 102100034343 Integrase Human genes 0.000 claims 6
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 6
- 102000023732 binding proteins Human genes 0.000 claims 6
- 108091008324 binding proteins Proteins 0.000 claims 6
- 238000006243 chemical reaction Methods 0.000 claims 5
- 230000000694 effects Effects 0.000 claims 5
- 150000003839 salts Chemical class 0.000 claims 5
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 claims 4
- 239000003795 chemical substances by application Substances 0.000 claims 4
- 230000000368 destabilizing effect Effects 0.000 claims 4
- 238000002844 melting Methods 0.000 claims 4
- 230000008018 melting Effects 0.000 claims 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 3
- 108020004414 DNA Proteins 0.000 claims 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims 2
- 102000053602 DNA Human genes 0.000 claims 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 2
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 claims 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 2
- 108010010677 Phosphodiesterase I Proteins 0.000 claims 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 2
- 108010006785 Taq Polymerase Proteins 0.000 claims 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims 2
- 229910052792 caesium Inorganic materials 0.000 claims 2
- TVFDJXOCXUVLDH-UHFFFAOYSA-N caesium atom Chemical compound [Cs] TVFDJXOCXUVLDH-UHFFFAOYSA-N 0.000 claims 2
- 230000000295 complement effect Effects 0.000 claims 2
- 229910052802 copper Inorganic materials 0.000 claims 2
- 239000010949 copper Substances 0.000 claims 2
- 238000006073 displacement reaction Methods 0.000 claims 2
- 229910052744 lithium Inorganic materials 0.000 claims 2
- 239000011777 magnesium Substances 0.000 claims 2
- 229910052749 magnesium Inorganic materials 0.000 claims 2
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims 2
- 229910052700 potassium Inorganic materials 0.000 claims 2
- 239000011591 potassium Substances 0.000 claims 2
- 229910052701 rubidium Inorganic materials 0.000 claims 2
- IGLNJRXAVVLDKE-UHFFFAOYSA-N rubidium atom Chemical compound [Rb] IGLNJRXAVVLDKE-UHFFFAOYSA-N 0.000 claims 2
- 229910052708 sodium Inorganic materials 0.000 claims 2
- 239000011734 sodium Substances 0.000 claims 2
- 239000011701 zinc Substances 0.000 claims 2
- 229910052725 zinc Inorganic materials 0.000 claims 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 1
- 108020004682 Single-Stranded DNA Proteins 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161477437P | 2011-04-20 | 2011-04-20 | |
| US201161477357P | 2011-04-20 | 2011-04-20 | |
| US61/477,437 | 2011-04-20 | ||
| US61/477,357 | 2011-04-20 | ||
| PCT/US2012/034589 WO2012145725A2 (en) | 2011-04-20 | 2012-04-20 | Oscillating amplification reaction for nucleic acids |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014513534A JP2014513534A (ja) | 2014-06-05 |
| JP2014513534A5 true JP2014513534A5 (enExample) | 2014-12-04 |
| JP5731066B2 JP5731066B2 (ja) | 2015-06-10 |
Family
ID=47042196
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014506611A Active JP5879667B2 (ja) | 2011-04-20 | 2012-04-20 | 核酸の検出および同定のための一体型デバイス |
| JP2014506607A Active JP5731066B2 (ja) | 2011-04-20 | 2012-04-20 | 核酸のための周期変動性増幅反応 |
| JP2015185214A Active JP6014227B2 (ja) | 2011-04-20 | 2015-09-18 | 核酸の検出および同定のための一体型デバイス |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014506611A Active JP5879667B2 (ja) | 2011-04-20 | 2012-04-20 | 核酸の検出および同定のための一体型デバイス |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015185214A Active JP6014227B2 (ja) | 2011-04-20 | 2015-09-18 | 核酸の検出および同定のための一体型デバイス |
Country Status (10)
| Country | Link |
|---|---|
| US (9) | US9428781B2 (enExample) |
| EP (2) | EP2699698B9 (enExample) |
| JP (3) | JP5879667B2 (enExample) |
| KR (3) | KR20140044323A (enExample) |
| CN (2) | CN103649333B (enExample) |
| CA (2) | CA2853615C (enExample) |
| DK (2) | DK2699698T4 (enExample) |
| ES (2) | ES2621390T5 (enExample) |
| PT (1) | PT2699698T (enExample) |
| WO (2) | WO2012145730A2 (enExample) |
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