JP2017508474A5 - - Google Patents
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- Publication number
- JP2017508474A5 JP2017508474A5 JP2016559274A JP2016559274A JP2017508474A5 JP 2017508474 A5 JP2017508474 A5 JP 2017508474A5 JP 2016559274 A JP2016559274 A JP 2016559274A JP 2016559274 A JP2016559274 A JP 2016559274A JP 2017508474 A5 JP2017508474 A5 JP 2017508474A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- nucleic acid
- acid template
- target nucleic
- atn
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 25
- 150000007523 nucleic acids Chemical class 0.000 claims 11
- 102000039446 nucleic acids Human genes 0.000 claims 11
- 108020004707 nucleic acids Proteins 0.000 claims 11
- 125000003729 nucleotide group Chemical group 0.000 claims 10
- 239000002773 nucleotide Substances 0.000 claims 7
- 150000003839 salts Chemical class 0.000 claims 7
- 239000011541 reaction mixture Substances 0.000 claims 6
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 4
- 238000006243 chemical reaction Methods 0.000 claims 4
- 239000003153 chemical reaction reagent Substances 0.000 claims 4
- 238000006073 displacement reaction Methods 0.000 claims 3
- YKBGVTZYEHREMT-KVQBGUIXSA-N 2'-deoxyguanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](CO)O1 YKBGVTZYEHREMT-KVQBGUIXSA-N 0.000 claims 2
- 239000002202 Polyethylene glycol Substances 0.000 claims 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 2
- 239000005549 deoxyribonucleoside Substances 0.000 claims 2
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 238000002844 melting Methods 0.000 claims 2
- 230000008018 melting Effects 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 229920001223 polyethylene glycol Polymers 0.000 claims 2
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims 2
- 235000011178 triphosphate Nutrition 0.000 claims 2
- 239000001226 triphosphate Substances 0.000 claims 2
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims 1
- NOLHIMIFXOBLFF-KVQBGUIXSA-N (2r,3s,5r)-5-(2,6-diaminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-ol Chemical group C12=NC(N)=NC(N)=C2N=CN1[C@H]1C[C@H](O)[C@@H](CO)O1 NOLHIMIFXOBLFF-KVQBGUIXSA-N 0.000 claims 1
- PISWNSOQFZRVJK-XLPZGREQSA-N 1-[(2r,4s,5r)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methyl-2-sulfanylidenepyrimidin-4-one Chemical compound S=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 PISWNSOQFZRVJK-XLPZGREQSA-N 0.000 claims 1
- CKTSBUTUHBMZGZ-SHYZEUOFSA-N 2'‐deoxycytidine Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-SHYZEUOFSA-N 0.000 claims 1
- CKTSBUTUHBMZGZ-UHFFFAOYSA-N Deoxycytidine Natural products O=C1N=C(N)C=CN1C1OC(CO)C(O)C1 CKTSBUTUHBMZGZ-UHFFFAOYSA-N 0.000 claims 1
- 229920001917 Ficoll Polymers 0.000 claims 1
- 239000008118 PEG 6000 Substances 0.000 claims 1
- 229920002565 Polyethylene Glycol 400 Polymers 0.000 claims 1
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 claims 1
- 229920002594 Polyethylene Glycol 8000 Polymers 0.000 claims 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 239000000539 dimer Substances 0.000 claims 1
- 238000011901 isothermal amplification Methods 0.000 claims 1
- JLFNLZLINWHATN-UHFFFAOYSA-N pentaethylene glycol Chemical compound OCCOCCOCCOCCOCCO JLFNLZLINWHATN-UHFFFAOYSA-N 0.000 claims 1
- 238000005096 rolling process Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14/225,887 US9587263B2 (en) | 2014-03-26 | 2014-03-26 | Isothermal amplification under low salt condition |
| US14/225,887 | 2014-03-26 | ||
| PCT/US2015/021272 WO2015148218A2 (en) | 2014-03-26 | 2015-03-18 | Isothermal amplification under low salt condition |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017508474A JP2017508474A (ja) | 2017-03-30 |
| JP2017508474A5 true JP2017508474A5 (enExample) | 2018-04-19 |
| JP7013069B2 JP7013069B2 (ja) | 2022-01-31 |
Family
ID=54189480
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016559274A Active JP7013069B2 (ja) | 2014-03-26 | 2015-03-18 | 低塩条件下での等温増幅 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US9587263B2 (enExample) |
| EP (1) | EP3122888B1 (enExample) |
| JP (1) | JP7013069B2 (enExample) |
| CN (1) | CN106460040B (enExample) |
| CA (1) | CA2939282C (enExample) |
| WO (1) | WO2015148218A2 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10443094B2 (en) * | 2014-03-26 | 2019-10-15 | General Electric Company | Solid phase isothermal amplification |
| WO2016011177A1 (en) * | 2014-07-18 | 2016-01-21 | Fluidigm Corporation | Reagents and kit compositions for single-cell whole genome amplification |
| CN111621548A (zh) * | 2016-04-26 | 2020-09-04 | 序康医疗科技（苏州）有限公司 | 扩增dna的方法 |
| TW201837184A (zh) * | 2016-12-19 | 2018-10-16 | 美商寬騰矽公司 | 分子載入樣品孔以供分析 |
| WO2019006321A1 (en) * | 2017-06-30 | 2019-01-03 | Circulomics, Inc. | PURIFICATION BY SIZE SELECTION USING THERMOPLASTIC SILICA NANOMATERIAL |
| US11230731B2 (en) * | 2018-04-02 | 2022-01-25 | Progenity, Inc. | Methods, systems, and compositions for counting nucleic acid molecules |
| GB201813429D0 (en) * | 2018-08-17 | 2018-10-03 | Touchlight Ip Ltd | Synthesis of DNA with improved yield |
| CN114174502B (zh) * | 2019-05-17 | 2025-09-19 | 4贝思生物有限公司 | 具有改进的引物识别的Phi29 DNA聚合酶突变体 |
| CN112899152B (zh) * | 2021-02-02 | 2023-11-17 | 中国科学院苏州纳米技术与纳米仿生研究所 | 核酸快速扩增和检测的微流控芯片、检测方法及系统 |
| CN114277103A (zh) * | 2022-01-21 | 2022-04-05 | 杭州飞时达生物科技有限公司 | 一种基于六聚体随机引物高效滚环扩增方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6096548A (en) | 1996-03-25 | 2000-08-01 | Maxygen, Inc. | Method for directing evolution of a virus |
| US6511803B1 (en) | 1997-10-10 | 2003-01-28 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
| DE19943374A1 (de) | 1999-09-10 | 2001-03-29 | Max Planck Gesellschaft | Verfahren zum Anbinden von Nukleinsäuren an eine Festphase |
| US7993839B2 (en) * | 2001-01-19 | 2011-08-09 | General Electric Company | Methods and kits for reducing non-specific nucleic acid amplification |
| US7662594B2 (en) * | 2002-09-20 | 2010-02-16 | New England Biolabs, Inc. | Helicase-dependent amplification of RNA |
| EP1583843B1 (en) * | 2002-12-20 | 2018-07-18 | QIAGEN GmbH | Single primer whole genome amplification |
| US7955795B2 (en) * | 2003-06-06 | 2011-06-07 | Qiagen Gmbh | Method of whole genome amplification with reduced artifact production |
| KR101077603B1 (ko) | 2004-01-28 | 2011-10-27 | 삼성전자주식회사 | 카르복실기 또는 아미노기로 코팅된 고체상 물질을 이용한핵산의 증폭 방법 |
| US8426126B2 (en) | 2004-03-18 | 2013-04-23 | Applied Biosystems, Llc | Modified surfaces as solid supports for nucleic acid purification |
| KR100785016B1 (ko) | 2006-05-22 | 2007-12-12 | 삼성전자주식회사 | 단일 마이크로 챔버에서 핵산의 농축 및 증폭을 수행하는방법 및 장치 |
| US20100184152A1 (en) * | 2006-10-23 | 2010-07-22 | Vladislav Sandler | Target-oriented whole genome amplification of nucleic acids |
| US20110195457A1 (en) * | 2010-02-09 | 2011-08-11 | General Electric Company | Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof |
| CN103003449A (zh) | 2010-05-06 | 2013-03-27 | 艾比斯生物科学公司 | 集成样品制备系统和稳定的酶混合物 |
| US9353393B2 (en) | 2012-02-15 | 2016-05-31 | General Electric Company | Methods and kits for reducing non-specific nucleic acid amplification |
-
2014
- 2014-03-26 US US14/225,887 patent/US9587263B2/en active Active
-
2015
- 2015-03-18 JP JP2016559274A patent/JP7013069B2/ja active Active
- 2015-03-18 EP EP15768311.1A patent/EP3122888B1/en active Active
- 2015-03-18 CA CA2939282A patent/CA2939282C/en active Active
- 2015-03-18 CN CN201580015896.3A patent/CN106460040B/zh active Active
- 2015-03-18 WO PCT/US2015/021272 patent/WO2015148218A2/en not_active Ceased
-
2017
- 2017-01-18 US US15/408,509 patent/US10597691B2/en active Active
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