JP2018518968A5 - - Google Patents
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- Publication number
- JP2018518968A5 JP2018518968A5 JP2017566728A JP2017566728A JP2018518968A5 JP 2018518968 A5 JP2018518968 A5 JP 2018518968A5 JP 2017566728 A JP2017566728 A JP 2017566728A JP 2017566728 A JP2017566728 A JP 2017566728A JP 2018518968 A5 JP2018518968 A5 JP 2018518968A5
- Authority
- JP
- Japan
- Prior art keywords
- dna molecules
- droplets
- target dna
- target
- molecules
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108020004414 DNA Proteins 0.000 claims 40
- 238000000034 method Methods 0.000 claims 17
- 230000003321 amplification Effects 0.000 claims 10
- 238000003199 nucleic acid amplification method Methods 0.000 claims 10
- 238000011895 specific detection Methods 0.000 claims 7
- 102000053602 DNA Human genes 0.000 claims 6
- 239000003153 chemical reaction reagent Substances 0.000 claims 6
- 239000007788 liquid Substances 0.000 claims 5
- 239000011541 reaction mixture Substances 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 3
- 102000039446 nucleic acids Human genes 0.000 claims 3
- 150000007523 nucleic acids Chemical class 0.000 claims 3
- 238000000338 in vitro Methods 0.000 claims 2
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 claims 1
- 239000012807 PCR reagent Substances 0.000 claims 1
- 230000000593 degrading effect Effects 0.000 claims 1
- 238000006073 displacement reaction Methods 0.000 claims 1
- 230000000415 inactivating effect Effects 0.000 claims 1
- 230000002779 inactivation Effects 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562185111P | 2015-06-26 | 2015-06-26 | |
| US62/185,111 | 2015-06-26 | ||
| DKPA201570449 | 2015-07-07 | ||
| DKPA201570449 | 2015-07-07 | ||
| PCT/EP2016/064719 WO2016207379A1 (en) | 2015-06-26 | 2016-06-24 | Targeted enrichment of long nucleotide sequences using microfluidic partitioning |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018518968A JP2018518968A (ja) | 2018-07-19 |
| JP2018518968A5 true JP2018518968A5 (enExample) | 2019-07-18 |
| JP6936155B2 JP6936155B2 (ja) | 2021-09-15 |
Family
ID=57584736
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017566728A Active JP6936155B2 (ja) | 2015-06-26 | 2016-06-24 | マイクロ流体分配を用いた長いヌクレオチド配列の標的富化 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10738354B2 (enExample) |
| EP (2) | EP3314012B1 (enExample) |
| JP (1) | JP6936155B2 (enExample) |
| AU (1) | AU2016282350A1 (enExample) |
| CA (1) | CA2990092A1 (enExample) |
| DK (1) | DK3770271T3 (enExample) |
| WO (1) | WO2016207379A1 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10704080B2 (en) * | 2014-06-11 | 2020-07-07 | Samplix Aps | Nucleotide sequence exclusion enrichment by droplet sorting (NEEDLS) |
| WO2018221513A1 (ja) * | 2017-05-31 | 2018-12-06 | キヤノン株式会社 | 油性組成物、およびそれを用いた分析方法 |
| DK182109B1 (en) | 2019-08-02 | 2025-08-18 | Samplix Aps | System and method for amplifying nucleic acids from single cells |
| US20210214769A1 (en) * | 2020-01-13 | 2021-07-15 | Fluent Biosciences Inc. | Methods and systems for amplifying low concentrations of nucleic acids |
| DK181994B1 (en) * | 2021-09-28 | 2025-05-15 | Samplix Aps | Instrument for sorting of particles |
| JP7734312B2 (ja) | 2020-12-01 | 2025-09-05 | サンプリックス エーピーエス | 粒子を分類するためのシステムおよび方法 |
| DK181862B1 (en) | 2022-03-21 | 2025-02-24 | Samplix Aps | Method and kit for targeted enrichment of large dna molecules for long-read sequencing using facs or microfluidic partitioning |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2977455B1 (en) | 2009-06-15 | 2020-04-15 | Complete Genomics, Inc. | Method for long fragment read sequencing |
| US20120164633A1 (en) | 2010-12-27 | 2012-06-28 | Ibis Biosciences, Inc. | Digital droplet sequencing |
| AU2013366478A1 (en) | 2012-12-21 | 2015-07-09 | Samplix S.A.R.L. | Probability-directed isolation of nucleotide sequences (pins) |
| US20140303005A1 (en) | 2013-04-05 | 2014-10-09 | Raindance Technologies, Inc. | Rare cell analysis after negative selection |
| WO2014172373A2 (en) | 2013-04-19 | 2014-10-23 | Raindance Technologies, Inc | Digital analyte analysis |
| US10704080B2 (en) * | 2014-06-11 | 2020-07-07 | Samplix Aps | Nucleotide sequence exclusion enrichment by droplet sorting (NEEDLS) |
| US10697007B2 (en) * | 2014-06-27 | 2020-06-30 | The Regents Of The University Of California | PCR-activated sorting (PAS) |
-
2016
- 2016-06-24 EP EP16732595.0A patent/EP3314012B1/en active Active
- 2016-06-24 DK DK20191574.1T patent/DK3770271T3/da active
- 2016-06-24 AU AU2016282350A patent/AU2016282350A1/en not_active Abandoned
- 2016-06-24 WO PCT/EP2016/064719 patent/WO2016207379A1/en not_active Ceased
- 2016-06-24 EP EP20191574.1A patent/EP3770271B1/en active Active
- 2016-06-24 CA CA2990092A patent/CA2990092A1/en active Pending
- 2016-06-24 JP JP2017566728A patent/JP6936155B2/ja active Active
- 2016-06-24 US US15/739,541 patent/US10738354B2/en active Active
-
2020
- 2020-07-06 US US16/921,103 patent/US11572584B2/en active Active
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