JP6936155B2 - マイクロ流体分配を用いた長いヌクレオチド配列の標的富化 - Google Patents

マイクロ流体分配を用いた長いヌクレオチド配列の標的富化 Download PDF

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JP6936155B2
JP6936155B2 JP2017566728A JP2017566728A JP6936155B2 JP 6936155 B2 JP6936155 B2 JP 6936155B2 JP 2017566728 A JP2017566728 A JP 2017566728A JP 2017566728 A JP2017566728 A JP 2017566728A JP 6936155 B2 JP6936155 B2 JP 6936155B2
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droplets
dna
target
target dna
droplet
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JP2018518968A (ja
JP2018518968A5 (enExample
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ユスト ミッケルセン マリー
ユスト ミッケルセン マリー
クビスト トマス
クビスト トマス
ビャアン マスン エスペン
ビャアン マスン エスペン
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サンプリクス アンパーツゼルスカブ
サンプリクス アンパーツゼルスカブ
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Dispersion Chemistry (AREA)
JP2017566728A 2015-06-26 2016-06-24 マイクロ流体分配を用いた長いヌクレオチド配列の標的富化 Active JP6936155B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562185111P 2015-06-26 2015-06-26
US62/185,111 2015-06-26
DKPA201570449 2015-07-07
DKPA201570449 2015-07-07
PCT/EP2016/064719 WO2016207379A1 (en) 2015-06-26 2016-06-24 Targeted enrichment of long nucleotide sequences using microfluidic partitioning

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JP2018518968A JP2018518968A (ja) 2018-07-19
JP2018518968A5 JP2018518968A5 (enExample) 2019-07-18
JP6936155B2 true JP6936155B2 (ja) 2021-09-15

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JP2017566728A Active JP6936155B2 (ja) 2015-06-26 2016-06-24 マイクロ流体分配を用いた長いヌクレオチド配列の標的富化

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US (2) US10738354B2 (enExample)
EP (2) EP3314012B1 (enExample)
JP (1) JP6936155B2 (enExample)
AU (1) AU2016282350A1 (enExample)
CA (1) CA2990092A1 (enExample)
DK (1) DK3770271T3 (enExample)
WO (1) WO2016207379A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10704080B2 (en) * 2014-06-11 2020-07-07 Samplix Aps Nucleotide sequence exclusion enrichment by droplet sorting (NEEDLS)
WO2018221513A1 (ja) * 2017-05-31 2018-12-06 キヤノン株式会社 油性組成物、およびそれを用いた分析方法
DK182109B1 (en) 2019-08-02 2025-08-18 Samplix Aps System and method for amplifying nucleic acids from single cells
US20210214769A1 (en) * 2020-01-13 2021-07-15 Fluent Biosciences Inc. Methods and systems for amplifying low concentrations of nucleic acids
DK181994B1 (en) * 2021-09-28 2025-05-15 Samplix Aps Instrument for sorting of particles
JP7734312B2 (ja) 2020-12-01 2025-09-05 サンプリックス エーピーエス 粒子を分類するためのシステムおよび方法
DK181862B1 (en) 2022-03-21 2025-02-24 Samplix Aps Method and kit for targeted enrichment of large dna molecules for long-read sequencing using facs or microfluidic partitioning

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2977455B1 (en) 2009-06-15 2020-04-15 Complete Genomics, Inc. Method for long fragment read sequencing
US20120164633A1 (en) 2010-12-27 2012-06-28 Ibis Biosciences, Inc. Digital droplet sequencing
AU2013366478A1 (en) 2012-12-21 2015-07-09 Samplix S.A.R.L. Probability-directed isolation of nucleotide sequences (pins)
US20140303005A1 (en) 2013-04-05 2014-10-09 Raindance Technologies, Inc. Rare cell analysis after negative selection
WO2014172373A2 (en) 2013-04-19 2014-10-23 Raindance Technologies, Inc Digital analyte analysis
US10704080B2 (en) * 2014-06-11 2020-07-07 Samplix Aps Nucleotide sequence exclusion enrichment by droplet sorting (NEEDLS)
US10697007B2 (en) * 2014-06-27 2020-06-30 The Regents Of The University Of California PCR-activated sorting (PAS)

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Publication number Publication date
EP3314012A1 (en) 2018-05-02
JP2018518968A (ja) 2018-07-19
US10738354B2 (en) 2020-08-11
US20200332353A1 (en) 2020-10-22
WO2016207379A1 (en) 2016-12-29
EP3770271B1 (en) 2023-02-08
US20180179589A1 (en) 2018-06-28
EP3314012B1 (en) 2020-09-09
CA2990092A1 (en) 2016-12-29
EP3770271A1 (en) 2021-01-27
US11572584B2 (en) 2023-02-07
AU2016282350A1 (en) 2018-01-18
DK3770271T3 (da) 2023-05-08

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