DK3770271T3 - Målrettet berigelse af lange nukleotidsekvenser under anvendelse af mikrofluidpartitionering - Google Patents
Målrettet berigelse af lange nukleotidsekvenser under anvendelse af mikrofluidpartitionering Download PDFInfo
- Publication number
- DK3770271T3 DK3770271T3 DK20191574.1T DK20191574T DK3770271T3 DK 3770271 T3 DK3770271 T3 DK 3770271T3 DK 20191574 T DK20191574 T DK 20191574T DK 3770271 T3 DK3770271 T3 DK 3770271T3
- Authority
- DK
- Denmark
- Prior art keywords
- nucleotide sequences
- targeted enrichment
- long nucleotide
- microfluidic partitioning
- microfluidic
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Dispersion Chemistry (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562185111P | 2015-06-26 | 2015-06-26 | |
DKPA201570449 | 2015-07-07 | ||
EP16732595.0A EP3314012B1 (en) | 2015-06-26 | 2016-06-24 | Targeted enrichment of long nucleotide sequences using microfluidic partitioning |
Publications (1)
Publication Number | Publication Date |
---|---|
DK3770271T3 true DK3770271T3 (da) | 2023-05-08 |
Family
ID=57584736
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK20191574.1T DK3770271T3 (da) | 2015-06-26 | 2016-06-24 | Målrettet berigelse af lange nukleotidsekvenser under anvendelse af mikrofluidpartitionering |
Country Status (7)
Country | Link |
---|---|
US (2) | US10738354B2 (da) |
EP (2) | EP3314012B1 (da) |
JP (1) | JP6936155B2 (da) |
AU (1) | AU2016282350A1 (da) |
CA (1) | CA2990092A1 (da) |
DK (1) | DK3770271T3 (da) |
WO (1) | WO2016207379A1 (da) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3155119B1 (en) * | 2014-06-11 | 2018-09-26 | Samplix ApS | Nucleotide sequence exclusion enrichment by droplet sorting (needls) |
JP2018203724A (ja) * | 2017-05-31 | 2018-12-27 | キヤノン株式会社 | 油性組成物、およびそれを用いた分析方法 |
DK201900926A1 (en) | 2019-08-02 | 2021-07-01 | Samplix Aps | System and method for amplifying nucleic acids from single cells |
WO2021146168A1 (en) * | 2020-01-13 | 2021-07-22 | Fluent Biosciences Inc. | Methods and systems for amplifying low concentrations of nucleic acids |
AU2021393736A1 (en) | 2020-12-01 | 2023-06-22 | Samplix Aps | System and method for sorting of particles |
DK202200230A1 (en) | 2022-03-21 | 2023-12-11 | Samplix Aps | Targeted enrichment of large dna molecules for long-read sequencing using facs or microfluidic partitioning |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107267596A (zh) | 2009-06-15 | 2017-10-20 | 考利达基因组股份有限公司 | 用于长片段阅读测序的方法和组合物 |
US20120164633A1 (en) | 2010-12-27 | 2012-06-28 | Ibis Biosciences, Inc. | Digital droplet sequencing |
AU2013366478A1 (en) | 2012-12-21 | 2015-07-09 | Samplix S.A.R.L. | Probability-directed isolation of nucleotide sequences (pins) |
US20140303005A1 (en) | 2013-04-05 | 2014-10-09 | Raindance Technologies, Inc. | Rare cell analysis after negative selection |
WO2014172373A2 (en) | 2013-04-19 | 2014-10-23 | Raindance Technologies, Inc | Digital analyte analysis |
EP3155119B1 (en) * | 2014-06-11 | 2018-09-26 | Samplix ApS | Nucleotide sequence exclusion enrichment by droplet sorting (needls) |
US10697007B2 (en) * | 2014-06-27 | 2020-06-30 | The Regents Of The University Of California | PCR-activated sorting (PAS) |
-
2016
- 2016-06-24 AU AU2016282350A patent/AU2016282350A1/en not_active Abandoned
- 2016-06-24 JP JP2017566728A patent/JP6936155B2/ja active Active
- 2016-06-24 DK DK20191574.1T patent/DK3770271T3/da active
- 2016-06-24 WO PCT/EP2016/064719 patent/WO2016207379A1/en active Application Filing
- 2016-06-24 US US15/739,541 patent/US10738354B2/en active Active
- 2016-06-24 EP EP16732595.0A patent/EP3314012B1/en active Active
- 2016-06-24 EP EP20191574.1A patent/EP3770271B1/en active Active
- 2016-06-24 CA CA2990092A patent/CA2990092A1/en active Pending
-
2020
- 2020-07-06 US US16/921,103 patent/US11572584B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
US20180179589A1 (en) | 2018-06-28 |
US20200332353A1 (en) | 2020-10-22 |
CA2990092A1 (en) | 2016-12-29 |
US10738354B2 (en) | 2020-08-11 |
EP3770271A1 (en) | 2021-01-27 |
EP3770271B1 (en) | 2023-02-08 |
US11572584B2 (en) | 2023-02-07 |
EP3314012A1 (en) | 2018-05-02 |
JP6936155B2 (ja) | 2021-09-15 |
AU2016282350A1 (en) | 2018-01-18 |
WO2016207379A1 (en) | 2016-12-29 |
EP3314012B1 (en) | 2020-09-09 |
JP2018518968A (ja) | 2018-07-19 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DK3445850T3 (da) | Fremgangsmåder til tilvejebringelse af enkeltstrenget rna | |
DK3334841T3 (da) | Fremgangsmåde til undersøgelse af nukleinsyrer | |
DK3448990T3 (da) | Forbedrede fremgangsmåder til modificering af målnukleinsyrer | |
DK3433368T3 (da) | Transreplikerende rna | |
DK3512850T3 (da) | Inhibitorer af menin-mll-interaktionen | |
DK3512959T3 (da) | Fremgangsmåder til udførelse af multipleks PCR | |
DK3337902T3 (da) | 3'-utr-sekvenser til stabilisering af rna | |
DK3468966T3 (da) | Inhibitorer af menin-mll-interaktionen | |
DK3341482T3 (da) | Kunstige nukleinsyremolekyler | |
DK3500682T3 (da) | Fremstilling af lukket, lineært dna | |
DK3161154T3 (da) | Modificerede polymeraser til forbedret inkorporering af nukleotidanaloger | |
DK3213084T4 (da) | Fremgangsmåde til berigelse af cirkulerende tumor dna | |
DK3292134T3 (da) | Metode til dyrkning af akkermansia | |
DK3374525T3 (da) | Højeffektiv konstruktion af dna-biblioteker | |
DK3134506T3 (da) | Fremgangsmåder til oprensning af messenger rna | |
DK3180331T3 (da) | Polymorfer af selinexor | |
DK3122897T3 (da) | Detektering af målnukleinsyresekvenser under anvendelse af forskellige detekteringstemperaturer | |
DK3307271T3 (da) | Fremgangsmåder til anvendelse af pyruvatkinase-aktivatorer | |
DK3201361T3 (da) | Fremgangsmåder til kvantificering af cellefrit DNA | |
DK3325443T3 (da) | Fremgangsmåde til fremstilling af pridopidin | |
DK3770271T3 (da) | Målrettet berigelse af lange nukleotidsekvenser under anvendelse af mikrofluidpartitionering | |
DK3344805T3 (da) | Fremgangsmåde til udvælgelse af aptamerer til ubundne mål | |
DK3186244T3 (da) | Dioxolananaloger af uridin til behandling af cancer | |
DK3351633T3 (da) | Antisense-nukleinsyre | |
DK3458100T3 (da) | Selektiv reduktion af cystein-manipulerede antistoffer |