DK3770271T3 - Målrettet berigelse af lange nukleotidsekvenser under anvendelse af mikrofluidpartitionering - Google Patents

Målrettet berigelse af lange nukleotidsekvenser under anvendelse af mikrofluidpartitionering Download PDF

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Publication number
DK3770271T3
DK3770271T3 DK20191574.1T DK20191574T DK3770271T3 DK 3770271 T3 DK3770271 T3 DK 3770271T3 DK 20191574 T DK20191574 T DK 20191574T DK 3770271 T3 DK3770271 T3 DK 3770271T3
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Denmark
Prior art keywords
nucleotide sequences
targeted enrichment
long nucleotide
microfluidic partitioning
microfluidic
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Application number
DK20191574.1T
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English (en)
Inventor
Marie Just Mikkelsen
Thomas Kvist
Esben Bjørn Madsen
Original Assignee
Samplix Aps
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Publication of DK3770271T3 publication Critical patent/DK3770271T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Dispersion Chemistry (AREA)
DK20191574.1T 2015-06-26 2016-06-24 Målrettet berigelse af lange nukleotidsekvenser under anvendelse af mikrofluidpartitionering DK3770271T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562185111P 2015-06-26 2015-06-26
DKPA201570449 2015-07-07
EP16732595.0A EP3314012B1 (en) 2015-06-26 2016-06-24 Targeted enrichment of long nucleotide sequences using microfluidic partitioning

Publications (1)

Publication Number Publication Date
DK3770271T3 true DK3770271T3 (da) 2023-05-08

Family

ID=57584736

Family Applications (1)

Application Number Title Priority Date Filing Date
DK20191574.1T DK3770271T3 (da) 2015-06-26 2016-06-24 Målrettet berigelse af lange nukleotidsekvenser under anvendelse af mikrofluidpartitionering

Country Status (7)

Country Link
US (2) US10738354B2 (da)
EP (2) EP3314012B1 (da)
JP (1) JP6936155B2 (da)
AU (1) AU2016282350A1 (da)
CA (1) CA2990092A1 (da)
DK (1) DK3770271T3 (da)
WO (1) WO2016207379A1 (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3155119B1 (en) * 2014-06-11 2018-09-26 Samplix ApS Nucleotide sequence exclusion enrichment by droplet sorting (needls)
JP2018203724A (ja) * 2017-05-31 2018-12-27 キヤノン株式会社 油性組成物、およびそれを用いた分析方法
DK201900926A1 (en) 2019-08-02 2021-07-01 Samplix Aps System and method for amplifying nucleic acids from single cells
WO2021146168A1 (en) * 2020-01-13 2021-07-22 Fluent Biosciences Inc. Methods and systems for amplifying low concentrations of nucleic acids
AU2021393736A1 (en) 2020-12-01 2023-06-22 Samplix Aps System and method for sorting of particles
DK202200230A1 (en) 2022-03-21 2023-12-11 Samplix Aps Targeted enrichment of large dna molecules for long-read sequencing using facs or microfluidic partitioning

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107267596A (zh) 2009-06-15 2017-10-20 考利达基因组股份有限公司 用于长片段阅读测序的方法和组合物
US20120164633A1 (en) 2010-12-27 2012-06-28 Ibis Biosciences, Inc. Digital droplet sequencing
AU2013366478A1 (en) 2012-12-21 2015-07-09 Samplix S.A.R.L. Probability-directed isolation of nucleotide sequences (pins)
US20140303005A1 (en) 2013-04-05 2014-10-09 Raindance Technologies, Inc. Rare cell analysis after negative selection
WO2014172373A2 (en) 2013-04-19 2014-10-23 Raindance Technologies, Inc Digital analyte analysis
EP3155119B1 (en) * 2014-06-11 2018-09-26 Samplix ApS Nucleotide sequence exclusion enrichment by droplet sorting (needls)
US10697007B2 (en) * 2014-06-27 2020-06-30 The Regents Of The University Of California PCR-activated sorting (PAS)

Also Published As

Publication number Publication date
US20180179589A1 (en) 2018-06-28
US20200332353A1 (en) 2020-10-22
CA2990092A1 (en) 2016-12-29
US10738354B2 (en) 2020-08-11
EP3770271A1 (en) 2021-01-27
EP3770271B1 (en) 2023-02-08
US11572584B2 (en) 2023-02-07
EP3314012A1 (en) 2018-05-02
JP6936155B2 (ja) 2021-09-15
AU2016282350A1 (en) 2018-01-18
WO2016207379A1 (en) 2016-12-29
EP3314012B1 (en) 2020-09-09
JP2018518968A (ja) 2018-07-19

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