AU2016282350A1 - Targeted enrichment of long nucleotide sequences using microfluidic partitioning - Google Patents

Targeted enrichment of long nucleotide sequences using microfluidic partitioning Download PDF

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Publication number
AU2016282350A1
AU2016282350A1 AU2016282350A AU2016282350A AU2016282350A1 AU 2016282350 A1 AU2016282350 A1 AU 2016282350A1 AU 2016282350 A AU2016282350 A AU 2016282350A AU 2016282350 A AU2016282350 A AU 2016282350A AU 2016282350 A1 AU2016282350 A1 AU 2016282350A1
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AU
Australia
Prior art keywords
dna
droplets
primer
target
target dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
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AU2016282350A
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English (en)
Inventor
Thomas Kvist
Esben Bjørn MADSEN
Marie Just Mikkelsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Samplix SARL
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Samplix SARL
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Publication date
Application filed by Samplix SARL filed Critical Samplix SARL
Publication of AU2016282350A1 publication Critical patent/AU2016282350A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Dispersion Chemistry (AREA)
AU2016282350A 2015-06-26 2016-06-24 Targeted enrichment of long nucleotide sequences using microfluidic partitioning Abandoned AU2016282350A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562185111P 2015-06-26 2015-06-26
US62/185,111 2015-06-26
DKPA201570449 2015-07-07
DKPA201570449 2015-07-07
PCT/EP2016/064719 WO2016207379A1 (en) 2015-06-26 2016-06-24 Targeted enrichment of long nucleotide sequences using microfluidic partitioning

Publications (1)

Publication Number Publication Date
AU2016282350A1 true AU2016282350A1 (en) 2018-01-18

Family

ID=57584736

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2016282350A Abandoned AU2016282350A1 (en) 2015-06-26 2016-06-24 Targeted enrichment of long nucleotide sequences using microfluidic partitioning

Country Status (7)

Country Link
US (2) US10738354B2 (enExample)
EP (2) EP3770271B1 (enExample)
JP (1) JP6936155B2 (enExample)
AU (1) AU2016282350A1 (enExample)
CA (1) CA2990092A1 (enExample)
DK (1) DK3770271T3 (enExample)
WO (1) WO2016207379A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015189336A1 (en) * 2014-06-11 2015-12-17 Samplix S.A.R.L. Nucleotide sequence exclusion enrichment by droplet sorting (needls)
JP2018203724A (ja) * 2017-05-31 2018-12-27 キヤノン株式会社 油性組成物、およびそれを用いた分析方法
DK182109B1 (en) 2019-08-02 2025-08-18 Samplix Aps System and method for amplifying nucleic acids from single cells
US20210214769A1 (en) * 2020-01-13 2021-07-15 Fluent Biosciences Inc. Methods and systems for amplifying low concentrations of nucleic acids
DK181994B1 (en) * 2021-09-28 2025-05-15 Samplix Aps Instrument for sorting of particles
EP4255632A1 (en) 2020-12-01 2023-10-11 Samplix ApS System and method for sorting of particles
DK181862B1 (en) * 2022-03-21 2025-02-24 Samplix Aps Method and kit for targeted enrichment of large dna molecules for long-read sequencing using facs or microfluidic partitioning

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107267596A (zh) 2009-06-15 2017-10-20 考利达基因组股份有限公司 用于长片段阅读测序的方法和组合物
US20120164633A1 (en) 2010-12-27 2012-06-28 Ibis Biosciences, Inc. Digital droplet sequencing
JP6522511B2 (ja) 2012-12-21 2019-05-29 サンプリクス アンパーツゼルスカブ ヌクレオチド配列の確率指向性単離(pins)
US20140303005A1 (en) 2013-04-05 2014-10-09 Raindance Technologies, Inc. Rare cell analysis after negative selection
WO2014172373A2 (en) 2013-04-19 2014-10-23 Raindance Technologies, Inc Digital analyte analysis
WO2015189336A1 (en) * 2014-06-11 2015-12-17 Samplix S.A.R.L. Nucleotide sequence exclusion enrichment by droplet sorting (needls)
EP3160654A4 (en) * 2014-06-27 2017-11-15 The Regents of The University of California Pcr-activated sorting (pas)

Also Published As

Publication number Publication date
EP3314012A1 (en) 2018-05-02
CA2990092A1 (en) 2016-12-29
US20200332353A1 (en) 2020-10-22
EP3770271B1 (en) 2023-02-08
EP3314012B1 (en) 2020-09-09
WO2016207379A1 (en) 2016-12-29
EP3770271A1 (en) 2021-01-27
US11572584B2 (en) 2023-02-07
JP6936155B2 (ja) 2021-09-15
JP2018518968A (ja) 2018-07-19
US20180179589A1 (en) 2018-06-28
DK3770271T3 (da) 2023-05-08
US10738354B2 (en) 2020-08-11

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Legal Events

Date Code Title Description
MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application