JP2015096066A5 - - Google Patents
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- Publication number
- JP2015096066A5 JP2015096066A5 JP2014242047A JP2014242047A JP2015096066A5 JP 2015096066 A5 JP2015096066 A5 JP 2015096066A5 JP 2014242047 A JP2014242047 A JP 2014242047A JP 2014242047 A JP2014242047 A JP 2014242047A JP 2015096066 A5 JP2015096066 A5 JP 2015096066A5
- Authority
- JP
- Japan
- Prior art keywords
- assay mixture
- seq
- sequence
- biological sample
- primer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000003556 assay Methods 0.000 claims 13
- 239000000203 mixture Substances 0.000 claims 13
- 239000012472 biological sample Substances 0.000 claims 5
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 4
- 239000002751 oligonucleotide probe Substances 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 3
- 150000007523 nucleic acids Chemical class 0.000 claims 3
- 102000039446 nucleic acids Human genes 0.000 claims 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 206010036790 Productive cough Diseases 0.000 claims 1
- 108010006785 Taq Polymerase Proteins 0.000 claims 1
- 101710182223 Toxin B Proteins 0.000 claims 1
- 210000000416 exudates and transudate Anatomy 0.000 claims 1
- 210000001165 lymph node Anatomy 0.000 claims 1
- 210000005259 peripheral blood Anatomy 0.000 claims 1
- 239000011886 peripheral blood Substances 0.000 claims 1
- 210000002381 plasma Anatomy 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 230000000241 respiratory effect Effects 0.000 claims 1
- 239000000523 sample Substances 0.000 claims 1
- 210000002966 serum Anatomy 0.000 claims 1
- 210000003802 sputum Anatomy 0.000 claims 1
- 208000024794 sputum Diseases 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US97049207P | 2007-09-06 | 2007-09-06 | |
| US60/970,492 | 2007-09-06 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010523247A Division JP5753381B2 (ja) | 2007-09-06 | 2008-09-05 | クロストリジウム・ディフィシレの毒素原性菌株の検出 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015096066A JP2015096066A (ja) | 2015-05-21 |
| JP2015096066A5 true JP2015096066A5 (enExample) | 2015-09-03 |
| JP5981979B2 JP5981979B2 (ja) | 2016-08-31 |
Family
ID=40428396
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010523247A Active JP5753381B2 (ja) | 2007-09-06 | 2008-09-05 | クロストリジウム・ディフィシレの毒素原性菌株の検出 |
| JP2014242047A Active JP5981979B2 (ja) | 2007-09-06 | 2014-11-28 | クロストリジウム・ディフィシレの毒素原性菌株の検出 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010523247A Active JP5753381B2 (ja) | 2007-09-06 | 2008-09-05 | クロストリジウム・ディフィシレの毒素原性菌株の検出 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US9096638B2 (enExample) |
| EP (1) | EP2195433B1 (enExample) |
| JP (2) | JP5753381B2 (enExample) |
| AU (1) | AU2008295396C1 (enExample) |
| CA (1) | CA2698232C (enExample) |
| WO (1) | WO2009030031A1 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9096638B2 (en) | 2007-09-06 | 2015-08-04 | Geneohm Sciences Canada, Inc. | Detection of toxigenic strains of Clostridium difficile |
| CN102378817B (zh) * | 2009-04-07 | 2015-09-30 | 皇家飞利浦电子股份有限公司 | 用于检测和表征产毒性艰难梭菌菌株的方法 |
| WO2011008942A2 (en) * | 2009-07-17 | 2011-01-20 | Brown University | Simultaneous quantitative multiple primer detection of clostridium difficile |
| US20110256535A1 (en) * | 2010-02-11 | 2011-10-20 | Intelligent Medical Devices, Inc. | Optimized oligonucleotides and methods of using same for the detection, isolation, amplification, quantification, monitoring, screening and sequencing of clostridium difficile genes encoding toxin b, and/or toxin a and/or binary toxin |
| MX391236B (es) | 2011-04-22 | 2025-03-21 | Wyeth Llc | Composiciones relacionadas con una toxina de clostridium difficile mutante y sus metodos. |
| CN103764850B (zh) * | 2011-09-01 | 2015-11-25 | 株式会社益力多本社 | 毒素产生性艰难梭菌的检测方法 |
| ES2948896T3 (es) * | 2012-03-29 | 2023-09-21 | Becton Dickinson Co | Acidos nucleicos para la amplificación de ácidos nucleicos |
| KR101955329B1 (ko) | 2012-06-08 | 2019-03-07 | 삼성전자주식회사 | 클로스트리디움 디피실리 균주 검출용 조성물과 키트 및 이를 이용한 검출 방법 |
| US9080217B2 (en) * | 2012-09-21 | 2015-07-14 | Roche Molecular Systems, Inc. | Methods for detection of Clostridium difficile |
| BR122016023101B1 (pt) | 2012-10-21 | 2022-03-22 | Pfizer Inc | Polipeptídeo, composição imunogênica que o compreende, bem como célula recombinante derivada de clostridium difficile |
| CN102952886A (zh) * | 2012-11-28 | 2013-03-06 | 中华人民共和国张家港出入境检验检疫局 | 艰难梭菌肠毒素a、b双重荧光定量pcr检测方法及检测用试剂盒 |
| US10227660B2 (en) | 2013-04-25 | 2019-03-12 | Orion Diagnostica Oy | Strand-invasion based DNA amplification method |
| JP7176266B2 (ja) * | 2018-07-18 | 2022-11-22 | 東洋紡株式会社 | トキシンＢ遺伝子（ｔｃｄＢ）を検出するためのオリゴヌクレオチドプローブ |
| US11597961B2 (en) | 2019-04-22 | 2023-03-07 | Qualitic Biotechnology LLC | Rapid colorimetric diagnostic test for C. difficile |
| CN115948584A (zh) * | 2022-12-16 | 2023-04-11 | 新疆维吾尔自治区疾病预防控制中心 | 利用CRISPR-Cas12a蛋白检测艰难梭菌的方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2388461C (en) * | 1999-09-28 | 2013-06-11 | Infectio Diagnostic (I.D.I.) Inc. | Highly conserved genes and their use to generate probes and primers for the detection of microorganisms |
| JP4899009B2 (ja) | 2005-06-02 | 2012-03-21 | 財団法人ヒューマンサイエンス振興財団 | ＬＡＭＰ法を用いたクロストリディウム・ディフィシルｔｏｘｉｎＢ遺伝子検出方法およびこの方法に用いるプライマーセット |
| US20100075305A1 (en) | 2006-10-03 | 2010-03-25 | Gifu University | Detection of bacterium by utilizing dnaj gene and use thereof |
| US9096638B2 (en) | 2007-09-06 | 2015-08-04 | Geneohm Sciences Canada, Inc. | Detection of toxigenic strains of Clostridium difficile |
-
2008
- 2008-09-03 US US12/203,694 patent/US9096638B2/en active Active
- 2008-09-05 AU AU2008295396A patent/AU2008295396C1/en active Active
- 2008-09-05 WO PCT/CA2008/001564 patent/WO2009030031A1/en not_active Ceased
- 2008-09-05 CA CA2698232A patent/CA2698232C/en active Active
- 2008-09-05 JP JP2010523247A patent/JP5753381B2/ja active Active
- 2008-09-05 EP EP08800272.0A patent/EP2195433B1/en active Active
-
2014
- 2014-11-28 JP JP2014242047A patent/JP5981979B2/ja active Active
-
2015
- 2015-06-26 US US14/752,586 patent/US9863007B2/en active Active
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