CA2698232C - Detection of toxigenic strains of clostridium difficile - Google Patents
Detection of toxigenic strains of clostridium difficile Download PDFInfo
- Publication number
- CA2698232C CA2698232C CA2698232A CA2698232A CA2698232C CA 2698232 C CA2698232 C CA 2698232C CA 2698232 A CA2698232 A CA 2698232A CA 2698232 A CA2698232 A CA 2698232A CA 2698232 C CA2698232 C CA 2698232C
- Authority
- CA
- Canada
- Prior art keywords
- pcr
- primer
- sequence
- seq
- primers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2561/00—Nucleic acid detection characterised by assay method
- C12Q2561/101—Taqman
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2561/00—Nucleic acid detection characterised by assay method
- C12Q2561/113—Real time assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US97049207P | 2007-09-06 | 2007-09-06 | |
| US60/970,492 | 2007-09-06 | ||
| PCT/CA2008/001564 WO2009030031A1 (en) | 2007-09-06 | 2008-09-05 | Detection of toxigenic strains of clostridium difficile |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2698232A1 CA2698232A1 (en) | 2009-03-12 |
| CA2698232C true CA2698232C (en) | 2018-06-12 |
Family
ID=40428396
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2698232A Active CA2698232C (en) | 2007-09-06 | 2008-09-05 | Detection of toxigenic strains of clostridium difficile |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US9096638B2 (enExample) |
| EP (1) | EP2195433B1 (enExample) |
| JP (2) | JP5753381B2 (enExample) |
| AU (1) | AU2008295396C1 (enExample) |
| CA (1) | CA2698232C (enExample) |
| WO (1) | WO2009030031A1 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9096638B2 (en) | 2007-09-06 | 2015-08-04 | Geneohm Sciences Canada, Inc. | Detection of toxigenic strains of Clostridium difficile |
| CN102378817B (zh) * | 2009-04-07 | 2015-09-30 | 皇家飞利浦电子股份有限公司 | 用于检测和表征产毒性艰难梭菌菌株的方法 |
| WO2011008942A2 (en) * | 2009-07-17 | 2011-01-20 | Brown University | Simultaneous quantitative multiple primer detection of clostridium difficile |
| US20110256535A1 (en) * | 2010-02-11 | 2011-10-20 | Intelligent Medical Devices, Inc. | Optimized oligonucleotides and methods of using same for the detection, isolation, amplification, quantification, monitoring, screening and sequencing of clostridium difficile genes encoding toxin b, and/or toxin a and/or binary toxin |
| MX391236B (es) | 2011-04-22 | 2025-03-21 | Wyeth Llc | Composiciones relacionadas con una toxina de clostridium difficile mutante y sus metodos. |
| CN103764850B (zh) * | 2011-09-01 | 2015-11-25 | 株式会社益力多本社 | 毒素产生性艰难梭菌的检测方法 |
| ES2948896T3 (es) * | 2012-03-29 | 2023-09-21 | Becton Dickinson Co | Acidos nucleicos para la amplificación de ácidos nucleicos |
| KR101955329B1 (ko) | 2012-06-08 | 2019-03-07 | 삼성전자주식회사 | 클로스트리디움 디피실리 균주 검출용 조성물과 키트 및 이를 이용한 검출 방법 |
| US9080217B2 (en) * | 2012-09-21 | 2015-07-14 | Roche Molecular Systems, Inc. | Methods for detection of Clostridium difficile |
| BR122016023101B1 (pt) | 2012-10-21 | 2022-03-22 | Pfizer Inc | Polipeptídeo, composição imunogênica que o compreende, bem como célula recombinante derivada de clostridium difficile |
| CN102952886A (zh) * | 2012-11-28 | 2013-03-06 | 中华人民共和国张家港出入境检验检疫局 | 艰难梭菌肠毒素a、b双重荧光定量pcr检测方法及检测用试剂盒 |
| US10227660B2 (en) | 2013-04-25 | 2019-03-12 | Orion Diagnostica Oy | Strand-invasion based DNA amplification method |
| JP7176266B2 (ja) * | 2018-07-18 | 2022-11-22 | 東洋紡株式会社 | トキシンＢ遺伝子（ｔｃｄＢ）を検出するためのオリゴヌクレオチドプローブ |
| US11597961B2 (en) | 2019-04-22 | 2023-03-07 | Qualitic Biotechnology LLC | Rapid colorimetric diagnostic test for C. difficile |
| CN115948584A (zh) * | 2022-12-16 | 2023-04-11 | 新疆维吾尔自治区疾病预防控制中心 | 利用CRISPR-Cas12a蛋白检测艰难梭菌的方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2388461C (en) * | 1999-09-28 | 2013-06-11 | Infectio Diagnostic (I.D.I.) Inc. | Highly conserved genes and their use to generate probes and primers for the detection of microorganisms |
| JP4899009B2 (ja) | 2005-06-02 | 2012-03-21 | 財団法人ヒューマンサイエンス振興財団 | ＬＡＭＰ法を用いたクロストリディウム・ディフィシルｔｏｘｉｎＢ遺伝子検出方法およびこの方法に用いるプライマーセット |
| US20100075305A1 (en) | 2006-10-03 | 2010-03-25 | Gifu University | Detection of bacterium by utilizing dnaj gene and use thereof |
| US9096638B2 (en) | 2007-09-06 | 2015-08-04 | Geneohm Sciences Canada, Inc. | Detection of toxigenic strains of Clostridium difficile |
-
2008
- 2008-09-03 US US12/203,694 patent/US9096638B2/en active Active
- 2008-09-05 AU AU2008295396A patent/AU2008295396C1/en active Active
- 2008-09-05 WO PCT/CA2008/001564 patent/WO2009030031A1/en not_active Ceased
- 2008-09-05 CA CA2698232A patent/CA2698232C/en active Active
- 2008-09-05 JP JP2010523247A patent/JP5753381B2/ja active Active
- 2008-09-05 EP EP08800272.0A patent/EP2195433B1/en active Active
-
2014
- 2014-11-28 JP JP2014242047A patent/JP5981979B2/ja active Active
-
2015
- 2015-06-26 US US14/752,586 patent/US9863007B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20150292004A1 (en) | 2015-10-15 |
| AU2008295396B2 (en) | 2015-01-29 |
| JP2015096066A (ja) | 2015-05-21 |
| AU2008295396A1 (en) | 2009-03-12 |
| CA2698232A1 (en) | 2009-03-12 |
| US9863007B2 (en) | 2018-01-09 |
| JP5981979B2 (ja) | 2016-08-31 |
| US20090208948A1 (en) | 2009-08-20 |
| AU2008295396C1 (en) | 2015-07-02 |
| EP2195433A1 (en) | 2010-06-16 |
| WO2009030031A1 (en) | 2009-03-12 |
| AU2008295396A2 (en) | 2010-05-13 |
| JP5753381B2 (ja) | 2015-07-22 |
| JP2010537648A (ja) | 2010-12-09 |
| EP2195433A4 (en) | 2011-05-18 |
| US9096638B2 (en) | 2015-08-04 |
| EP2195433B1 (en) | 2014-12-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20130806 |