JP2015526069A5 - - Google Patents
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- Publication number
- JP2015526069A5 JP2015526069A5 JP2015520511A JP2015520511A JP2015526069A5 JP 2015526069 A5 JP2015526069 A5 JP 2015526069A5 JP 2015520511 A JP2015520511 A JP 2015520511A JP 2015520511 A JP2015520511 A JP 2015520511A JP 2015526069 A5 JP2015526069 A5 JP 2015526069A5
- Authority
- JP
- Japan
- Prior art keywords
- inosine
- reverse transcriptase
- dna
- primers
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 13
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 claims 7
- 229920002477 rna polymer Polymers 0.000 claims 7
- 239000011541 reaction mixture Substances 0.000 claims 6
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 5
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 5
- 229930010555 Inosine Natural products 0.000 claims 5
- 229960003786 inosine Drugs 0.000 claims 5
- 108020004414 DNA Proteins 0.000 claims 4
- 102000053602 DNA Human genes 0.000 claims 4
- 102100031780 Endonuclease Human genes 0.000 claims 4
- 101710163270 Nuclease Proteins 0.000 claims 4
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 4
- 239000002299 complementary DNA Substances 0.000 claims 4
- 108010082610 Deoxyribonuclease (Pyrimidine Dimer) Proteins 0.000 claims 3
- 102100037696 Endonuclease V Human genes 0.000 claims 3
- 238000006073 displacement reaction Methods 0.000 claims 3
- 102000052510 DNA-Binding Proteins Human genes 0.000 claims 2
- 108700020911 DNA-Binding Proteins Proteins 0.000 claims 2
- 108060002716 Exonuclease Proteins 0.000 claims 2
- 108091093037 Peptide nucleic acid Proteins 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 2
- 230000002950 deficient Effects 0.000 claims 2
- 102000013165 exonuclease Human genes 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 238000010839 reverse transcription Methods 0.000 claims 2
- 108700014590 single-stranded DNA binding proteins Proteins 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 108010078851 HIV Reverse Transcriptase Proteins 0.000 claims 1
- 241000713869 Moloney murine leukemia virus Species 0.000 claims 1
- 238000000137 annealing Methods 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims 1
- 235000011178 triphosphate Nutrition 0.000 claims 1
- 239000001226 triphosphate Substances 0.000 claims 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/538,955 US9777319B2 (en) | 2012-06-29 | 2012-06-29 | Method for isothermal DNA amplification starting from an RNA template |
| US13/538,955 | 2012-06-29 | ||
| US13/538,962 US20140004509A1 (en) | 2012-06-29 | 2012-06-29 | Kit for isothermal dna amplification starting from an rna template |
| US13/538,962 | 2012-06-29 | ||
| PCT/US2013/048229 WO2014004852A2 (en) | 2012-06-29 | 2013-06-27 | Kit for isothermal dna amplification starting from an rna template |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015526069A JP2015526069A (ja) | 2015-09-10 |
| JP2015526069A5 true JP2015526069A5 (enExample) | 2016-08-12 |
| JP6382189B2 JP6382189B2 (ja) | 2018-08-29 |
Family
ID=49784019
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015520511A Active JP6382189B2 (ja) | 2012-06-29 | 2013-06-27 | Ｒｎａテンプレートから開始する等温ｄｎａ増幅用のキット |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP2867366B1 (enExample) |
| JP (1) | JP6382189B2 (enExample) |
| CN (1) | CN104583413B (enExample) |
| CA (1) | CA2877368C (enExample) |
| WO (1) | WO2014004852A2 (enExample) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130323795A1 (en) | 2007-01-10 | 2013-12-05 | General Electric Company | Endonuclase-assisted isothermal amplification using contamination-free reagents |
| BR112013004044A2 (pt) | 2010-08-13 | 2016-07-05 | Envirologix Inc | composições e métodos para quantificação de uma sequência de ácido nucleico em uma amostra. |
| KR102159818B1 (ko) | 2012-04-09 | 2020-09-25 | 엔바이로로직스 인크. | 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법 |
| EP3033431B1 (en) * | 2013-08-13 | 2019-10-02 | General Electric Company | Endonuclease-assisted isothermal amplification using contamination-free reagents |
| CN106164298B (zh) * | 2014-02-18 | 2020-02-21 | 伊鲁米那股份有限公司 | 用于dna谱系分析的方法及组合物 |
| US11505836B2 (en) | 2014-04-22 | 2022-11-22 | Envirologix Inc. | Compositions and methods for enhancing and/or predicting DNA amplification |
| AU2015336086C1 (en) * | 2014-10-20 | 2021-07-29 | Envirologix Inc. | Compositions and methods for detecting an RNA virus |
| EP3240906B1 (en) | 2014-12-31 | 2021-08-25 | Visby Medical, Inc. | Devices for molecular diagnostic testing |
| SG11201810209VA (en) * | 2016-05-17 | 2018-12-28 | Japan Science & Tech Agency | Method of amplifying circular dna |
| CN107400701A (zh) * | 2016-05-18 | 2017-11-28 | 上海德沛生物科技有限公司 | 环状rna分子的扩增和测序方法 |
| CN107541507A (zh) * | 2016-06-29 | 2018-01-05 | 厦门大学 | 一种rna反转录扩增方法 |
| WO2018005870A1 (en) * | 2016-06-30 | 2018-01-04 | Click Diagnostics, Inc. | Devices and methods for nucleic acid extraction |
| US10350307B2 (en) * | 2017-09-18 | 2019-07-16 | General Electric Company | In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides |
| WO2019094784A1 (en) | 2017-11-09 | 2019-05-16 | Click Diagnostics, Inc. | Portable molecular diagnostic device and methods for the detection of target viruses |
| JP7029138B2 (ja) * | 2018-03-05 | 2022-03-03 | 国立大学法人埼玉大学 | 連結核酸断片の製造方法、連結核酸断片、及び連結核酸断片から構成されるライブラリ |
| CN110819673B (zh) * | 2018-08-07 | 2021-07-13 | 华中农业大学 | 一种原核生物cDNA5′末端快速扩增方法 |
| WO2020132714A1 (en) * | 2018-12-24 | 2020-07-02 | Deakin University | Method for amplification of nucleic acids |
| CN114891860A (zh) * | 2022-03-31 | 2022-08-12 | 河南省人民医院 | 一种基于crispr和hrca技术的可视化靶dna检测方法 |
| CN114921528A (zh) * | 2022-04-22 | 2022-08-19 | 山东见微生物科技有限公司 | 一种核酸内切酶4介导的rna特异扩增方法 |
| CN116004681B (zh) * | 2022-08-15 | 2023-08-29 | 南京诺唯赞生物科技股份有限公司 | 一种提高topo克隆中载体连接效率的方法及试剂盒 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6977153B2 (en) * | 2002-12-31 | 2005-12-20 | Qiagen Gmbh | Rolling circle amplification of RNA |
| WO2006090073A2 (fr) * | 2005-02-25 | 2006-08-31 | Comissariat A L'energie Atomique | Procede et dispositif pour separer des cibles moleculaires dans un melange complexe |
| JP2008541705A (ja) * | 2005-05-26 | 2008-11-27 | ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド | 非標準塩基を含むプライマーを使用する等温鎖置換増幅 |
| JP4918409B2 (ja) * | 2006-07-26 | 2012-04-18 | 西川ゴム工業株式会社 | 核酸配列の増幅方法 |
| US8202972B2 (en) * | 2007-01-10 | 2012-06-19 | General Electric Company | Isothermal DNA amplification |
| AU2010206492B2 (en) * | 2009-01-21 | 2012-07-12 | Human Genetic Signatures Pty Ltd | Improved isothermal strand displacement amplification |
| JP2012080871A (ja) * | 2009-12-14 | 2012-04-26 | National Agriculture & Food Research Organization | Ｒｎａの直接検出法 |
| WO2014164479A1 (en) * | 2013-03-11 | 2014-10-09 | Elitech Holding B.V. | Methods for true isothermal strand displacement amplification |
-
2013
- 2013-06-27 WO PCT/US2013/048229 patent/WO2014004852A2/en not_active Ceased
- 2013-06-27 EP EP13809883.5A patent/EP2867366B1/en active Active
- 2013-06-27 CA CA2877368A patent/CA2877368C/en active Active
- 2013-06-27 CN CN201380044858.1A patent/CN104583413B/zh active Active
- 2013-06-27 JP JP2015520511A patent/JP6382189B2/ja active Active
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