CA2877368C - Kit for isothermal dna amplification starting from an rna template - Google Patents
Kit for isothermal dna amplification starting from an rna template Download PDFInfo
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- CA2877368C CA2877368C CA2877368A CA2877368A CA2877368C CA 2877368 C CA2877368 C CA 2877368C CA 2877368 A CA2877368 A CA 2877368A CA 2877368 A CA2877368 A CA 2877368A CA 2877368 C CA2877368 C CA 2877368C
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- dna
- rna
- template
- primer
- inosine
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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| US13/538,955 US9777319B2 (en) | 2012-06-29 | 2012-06-29 | Method for isothermal DNA amplification starting from an RNA template |
| US13/538,955 | 2012-06-29 | ||
| PCT/US2013/048229 WO2014004852A2 (en) | 2012-06-29 | 2013-06-27 | Kit for isothermal dna amplification starting from an rna template |
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| US20130323795A1 (en) | 2007-01-10 | 2013-12-05 | General Electric Company | Endonuclase-assisted isothermal amplification using contamination-free reagents |
| CA2810856C (en) | 2010-08-13 | 2022-05-17 | Envirologix Inc. | Compositions and methods for quantifying a nucleic acid sequence in a sample |
| BR112014025262A8 (pt) | 2012-04-09 | 2021-06-29 | Envirologix Inc | métodos para quantificar um produto específico em uma reação de amplificação de extensão e corte, para detectar uma pluralidade de produtos de reação distintos produzidos ao longo de uma única reação, para monitorar em tempo real uma reação de amplificação de extensão e corte, para monitorar em tempo real uma molécula-alvo de ácido nucleico em uma reação de amplificação de extensão e corte, para monitorar em tempo real uma molécula-alvo de ácido nucleico em uma amostra de teste, e para amplificar um polinucleotídeo, kit, e, oligonucleotídeo isolado |
| WO2015022359A1 (en) * | 2013-08-13 | 2015-02-19 | General Electric Company | Endonuclease-assisted isothermal amplification using contamination-free reagents |
| RS61976B1 (sr) * | 2014-02-18 | 2021-07-30 | Illumina Inc | Postupci i kompozicije za određivanje dnk profila |
| WO2015164494A1 (en) | 2014-04-22 | 2015-10-29 | Envirologix, Inc. | Compositions and methods for enhancing and/or predicting dna amplification |
| US10793922B2 (en) | 2014-10-20 | 2020-10-06 | Envirologix Inc. | Compositions and methods for detecting an RNA virus |
| AU2015373998A1 (en) | 2014-12-31 | 2017-06-29 | Visby Medical, Inc. | Devices and methods for molecular diagnostic testing |
| US12157915B2 (en) * | 2016-05-17 | 2024-12-03 | Moderna Enzymatics Co., Ltd. | Method of amplifying circular DNA |
| CN107400701A (zh) * | 2016-05-18 | 2017-11-28 | 上海德沛生物科技有限公司 | 环状rna分子的扩增和测序方法 |
| CN107541507A (zh) * | 2016-06-29 | 2018-01-05 | 厦门大学 | 一种rna反转录扩增方法 |
| EP3478417A4 (en) * | 2016-06-30 | 2020-01-15 | Click Diagnostics, Inc. | DEVICES AND METHOD FOR NUCLEIC ACID EXTRACTION |
| US10350307B2 (en) * | 2017-09-18 | 2019-07-16 | General Electric Company | In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides |
| JP7239568B2 (ja) | 2017-11-09 | 2023-03-14 | ビスビュー メディカル，インコーポレイテッド | 携帯型分子診断デバイスおよび標的ウイルスの検出方法 |
| JP7029138B2 (ja) * | 2018-03-05 | 2022-03-03 | 国立大学法人埼玉大学 | 連結核酸断片の製造方法、連結核酸断片、及び連結核酸断片から構成されるライブラリ |
| CN110819673B (zh) * | 2018-08-07 | 2021-07-13 | 华中农业大学 | 一种原核生物cDNA5′末端快速扩增方法 |
| WO2020132714A1 (en) * | 2018-12-24 | 2020-07-02 | Deakin University | Method for amplification of nucleic acids |
| CN114891860A (zh) * | 2022-03-31 | 2022-08-12 | 河南省人民医院 | 一种基于crispr和hrca技术的可视化靶dna检测方法 |
| CN114921528A (zh) * | 2022-04-22 | 2022-08-19 | 山东见微生物科技有限公司 | 一种核酸内切酶4介导的rna特异扩增方法 |
| CN116004681B (zh) * | 2022-08-15 | 2023-08-29 | 南京诺唯赞生物科技股份有限公司 | 一种提高topo克隆中载体连接效率的方法及试剂盒 |
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| US6977153B2 (en) * | 2002-12-31 | 2005-12-20 | Qiagen Gmbh | Rolling circle amplification of RNA |
| EP1851331B1 (fr) * | 2005-02-25 | 2016-02-24 | Commissariat à l'Énergie Atomique et aux Énergies Alternatives | Procede et dispositif pour separer des cibles moleculaires dans un melange complexe |
| WO2006125267A1 (en) * | 2005-05-26 | 2006-11-30 | Human Genetic Signatures Pty Ltd | Isothermal strand displacement amplification using primers containing a non-regular base |
| JP4918409B2 (ja) * | 2006-07-26 | 2012-04-18 | 西川ゴム工業株式会社 | 核酸配列の増幅方法 |
| US8202972B2 (en) * | 2007-01-10 | 2012-06-19 | General Electric Company | Isothermal DNA amplification |
| EP2379719A4 (en) * | 2009-01-21 | 2012-10-03 | Human Genetic Signatures Pty | IMPROVED ISOTHERMAL STRANDSHIFT REINFORCEMENT |
| JP2012080871A (ja) * | 2009-12-14 | 2012-04-26 | National Agriculture & Food Research Organization | Ｒｎａの直接検出法 |
| US20140255928A1 (en) * | 2013-03-11 | 2014-09-11 | Elitech Holding B.V. | Methods for true isothermal strand displacement amplification |
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| EP2867366A2 (en) | 2015-05-06 |
| EP2867366B1 (en) | 2018-05-16 |
| JP2015526069A (ja) | 2015-09-10 |
| JP6382189B2 (ja) | 2018-08-29 |
| WO2014004852A2 (en) | 2014-01-03 |
| EP2867366A4 (en) | 2016-04-20 |
| WO2014004852A3 (en) | 2014-04-24 |
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