CA2877368C - Kit for isothermal dna amplification starting from an rna template - Google Patents

Kit for isothermal dna amplification starting from an rna template Download PDF

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Publication number
CA2877368C
CA2877368C CA2877368A CA2877368A CA2877368C CA 2877368 C CA2877368 C CA 2877368C CA 2877368 A CA2877368 A CA 2877368A CA 2877368 A CA2877368 A CA 2877368A CA 2877368 C CA2877368 C CA 2877368C
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CA
Canada
Prior art keywords
dna
rna
template
primer
inosine
Prior art date
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Active
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CA2877368A
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English (en)
French (fr)
Other versions
CA2877368A1 (en
Inventor
John Richard Nelson
Robert Scott Duthie
Gregory Andrew Grossmann
Ryan Charles HELLER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions Operations UK Ltd
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Global Life Sciences Solutions Operations UK Ltd
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Filing date
Publication date
Priority claimed from US13/538,955 external-priority patent/US9777319B2/en
Priority claimed from US13/538,962 external-priority patent/US20140004509A1/en
Application filed by Global Life Sciences Solutions Operations UK Ltd filed Critical Global Life Sciences Solutions Operations UK Ltd
Publication of CA2877368A1 publication Critical patent/CA2877368A1/en
Application granted granted Critical
Publication of CA2877368C publication Critical patent/CA2877368C/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2877368A 2012-06-29 2013-06-27 Kit for isothermal dna amplification starting from an rna template Active CA2877368C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US13/538,955 US9777319B2 (en) 2012-06-29 2012-06-29 Method for isothermal DNA amplification starting from an RNA template
US13/538,962 US20140004509A1 (en) 2012-06-29 2012-06-29 Kit for isothermal dna amplification starting from an rna template
US13/538,962 2012-06-29
US13/538,955 2012-06-29
PCT/US2013/048229 WO2014004852A2 (en) 2012-06-29 2013-06-27 Kit for isothermal dna amplification starting from an rna template

Publications (2)

Publication Number Publication Date
CA2877368A1 CA2877368A1 (en) 2014-01-03
CA2877368C true CA2877368C (en) 2021-07-13

Family

ID=49784019

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2877368A Active CA2877368C (en) 2012-06-29 2013-06-27 Kit for isothermal dna amplification starting from an rna template

Country Status (5)

Country Link
EP (1) EP2867366B1 (enExample)
JP (1) JP6382189B2 (enExample)
CN (1) CN104583413B (enExample)
CA (1) CA2877368C (enExample)
WO (1) WO2014004852A2 (enExample)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130323795A1 (en) 2007-01-10 2013-12-05 General Electric Company Endonuclase-assisted isothermal amplification using contamination-free reagents
CA2810856C (en) 2010-08-13 2022-05-17 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample
NZ723570A (en) 2012-04-09 2018-06-29 Envirologix Inc Compositions and methods for quantifying a nucleic acid sequence in a sample
WO2015022359A1 (en) * 2013-08-13 2015-02-19 General Electric Company Endonuclease-assisted isothermal amplification using contamination-free reagents
SI3108009T1 (sl) * 2014-02-18 2021-09-30 Illumina, Inc. Postopki in sestavki za profiliranje DNK
CN106460071B (zh) 2014-04-22 2021-09-10 一龙公司 增强和/或预测dna扩增的组合物和方法
BR112017008082A2 (pt) * 2014-10-20 2017-12-26 Envirologix Inc composições e métodos para detectar um vírus de rna
US9623415B2 (en) 2014-12-31 2017-04-18 Click Diagnostics, Inc. Devices and methods for molecular diagnostic testing
KR102378346B1 (ko) * 2016-05-17 2022-03-25 오리시로 제노믹스 가부시키가이샤 환상 dna의 증폭 방법
CN107400701A (zh) * 2016-05-18 2017-11-28 上海德沛生物科技有限公司 环状rna分子的扩增和测序方法
CN107541507A (zh) * 2016-06-29 2018-01-05 厦门大学 一种rna反转录扩增方法
CN109661273B (zh) * 2016-06-30 2022-11-04 维斯比医学公司 用于核酸提取的装置和方法
US10350307B2 (en) * 2017-09-18 2019-07-16 General Electric Company In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides
MX2020004783A (es) 2017-11-09 2020-08-13 Visby Medical Inc Dispositivo portatil para el diagnostico molecular y metodos para la deteccion de virus objetivo.
JP7029138B2 (ja) * 2018-03-05 2022-03-03 国立大学法人埼玉大学 連結核酸断片の製造方法、連結核酸断片、及び連結核酸断片から構成されるライブラリ
CN110819673B (zh) * 2018-08-07 2021-07-13 华中农业大学 一种原核生物cDNA5′末端快速扩增方法
WO2020132714A1 (en) * 2018-12-24 2020-07-02 Deakin University Method for amplification of nucleic acids
CA3214584A1 (en) * 2021-03-30 2022-10-06 Illumina, Inc. Improved methods of isothermal complementary dna and library preparation
CN114891860A (zh) * 2022-03-31 2022-08-12 河南省人民医院 一种基于crispr和hrca技术的可视化靶dna检测方法
CN114921528A (zh) * 2022-04-22 2022-08-19 山东见微生物科技有限公司 一种核酸内切酶4介导的rna特异扩增方法
CN116004681B (zh) * 2022-08-15 2023-08-29 南京诺唯赞生物科技股份有限公司 一种提高topo克隆中载体连接效率的方法及试剂盒

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6977153B2 (en) * 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
CA2598508A1 (fr) * 2005-02-25 2006-08-31 Commissariat A L'energie Atomique Procede et dispositif pour separer des cibles moleculaires dans un melange complexe
EP1883707B1 (en) * 2005-05-26 2014-04-09 Human Genetic Signatures PTY Ltd Isothermal strand displacement amplification using primers containing a non-regular base
JP4918409B2 (ja) * 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
US8202972B2 (en) * 2007-01-10 2012-06-19 General Electric Company Isothermal DNA amplification
EP2379719A4 (en) * 2009-01-21 2012-10-03 Human Genetic Signatures Pty IMPROVED ISOTHERMAL STRANDSHIFT REINFORCEMENT
JP2012080871A (ja) * 2009-12-14 2012-04-26 National Agriculture & Food Research Organization Ｒｎａの直接検出法
WO2014164479A1 (en) * 2013-03-11 2014-10-09 Elitech Holding B.V. Methods for true isothermal strand displacement amplification

Also Published As

Publication number Publication date
WO2014004852A2 (en) 2014-01-03
CA2877368A1 (en) 2014-01-03
CN104583413A (zh) 2015-04-29
CN104583413B (zh) 2018-10-19
WO2014004852A3 (en) 2014-04-24
JP2015526069A (ja) 2015-09-10
JP6382189B2 (ja) 2018-08-29
EP2867366B1 (en) 2018-05-16
EP2867366A4 (en) 2016-04-20
EP2867366A2 (en) 2015-05-06

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