JP6382189B2 - Ｒｎａテンプレートから開始する等温ｄｎａ増幅用のキット - Google Patents

Ｒｎａテンプレートから開始する等温ｄｎａ増幅用のキット Download PDF

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Publication number
JP6382189B2
JP6382189B2 JP2015520511A JP2015520511A JP6382189B2 JP 6382189 B2 JP6382189 B2 JP 6382189B2 JP 2015520511 A JP2015520511 A JP 2015520511A JP 2015520511 A JP2015520511 A JP 2015520511A JP 6382189 B2 JP6382189 B2 JP 6382189B2
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Prior art keywords
dna
rna
cdna
template
primer
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Japanese (ja)
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JP2015526069A5 (enExample
JP2015526069A (ja
Inventor
ネルソン，ジョン・リチャード
デュシー，ロバート・スコット
グロスマン，グレゴリー・アンドリュー
ヘラー，ライアン・チャールズ
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General Electric Co
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General Electric Co
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Priority claimed from US13/538,955 external-priority patent/US9777319B2/en
Priority claimed from US13/538,962 external-priority patent/US20140004509A1/en
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Publication of JP2015526069A publication Critical patent/JP2015526069A/ja
Publication of JP2015526069A5 publication Critical patent/JP2015526069A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2015520511A 2012-06-29 2013-06-27 Ｒｎａテンプレートから開始する等温ｄｎａ増幅用のキット Active JP6382189B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US13/538,955 US9777319B2 (en) 2012-06-29 2012-06-29 Method for isothermal DNA amplification starting from an RNA template
US13/538,955 2012-06-29
US13/538,962 US20140004509A1 (en) 2012-06-29 2012-06-29 Kit for isothermal dna amplification starting from an rna template
US13/538,962 2012-06-29
PCT/US2013/048229 WO2014004852A2 (en) 2012-06-29 2013-06-27 Kit for isothermal dna amplification starting from an rna template

Publications (3)

Publication Number Publication Date
JP2015526069A JP2015526069A (ja) 2015-09-10
JP2015526069A5 JP2015526069A5 (enExample) 2016-08-12
JP6382189B2 true JP6382189B2 (ja) 2018-08-29

Family

ID=49784019

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015520511A Active JP6382189B2 (ja) 2012-06-29 2013-06-27 Ｒｎａテンプレートから開始する等温ｄｎａ増幅用のキット

Country Status (5)

Country Link
EP (1) EP2867366B1 (enExample)
JP (1) JP6382189B2 (enExample)
CN (1) CN104583413B (enExample)
CA (1) CA2877368C (enExample)
WO (1) WO2014004852A2 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130323795A1 (en) 2007-01-10 2013-12-05 General Electric Company Endonuclase-assisted isothermal amplification using contamination-free reagents
BR112013004044A2 (pt) 2010-08-13 2016-07-05 Envirologix Inc composições e métodos para quantificação de uma sequência de ácido nucleico em uma amostra.
KR102159818B1 (ko) 2012-04-09 2020-09-25 엔바이로로직스 인크. 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법
EP3033431B1 (en) * 2013-08-13 2019-10-02 General Electric Company Endonuclease-assisted isothermal amplification using contamination-free reagents
CN106164298B (zh) * 2014-02-18 2020-02-21 伊鲁米那股份有限公司 用于dna谱系分析的方法及组合物
US11505836B2 (en) 2014-04-22 2022-11-22 Envirologix Inc. Compositions and methods for enhancing and/or predicting DNA amplification
AU2015336086C1 (en) * 2014-10-20 2021-07-29 Envirologix Inc. Compositions and methods for detecting an RNA virus
EP3240906B1 (en) 2014-12-31 2021-08-25 Visby Medical, Inc. Devices for molecular diagnostic testing
SG11201810209VA (en) * 2016-05-17 2018-12-28 Japan Science & Tech Agency Method of amplifying circular dna
CN107400701A (zh) * 2016-05-18 2017-11-28 上海德沛生物科技有限公司 环状rna分子的扩增和测序方法
CN107541507A (zh) * 2016-06-29 2018-01-05 厦门大学 一种rna反转录扩增方法
WO2018005870A1 (en) * 2016-06-30 2018-01-04 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
US10350307B2 (en) * 2017-09-18 2019-07-16 General Electric Company In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides
WO2019094784A1 (en) 2017-11-09 2019-05-16 Click Diagnostics, Inc. Portable molecular diagnostic device and methods for the detection of target viruses
JP7029138B2 (ja) * 2018-03-05 2022-03-03 国立大学法人埼玉大学 連結核酸断片の製造方法、連結核酸断片、及び連結核酸断片から構成されるライブラリ
CN110819673B (zh) * 2018-08-07 2021-07-13 华中农业大学 一种原核生物cDNA5′末端快速扩增方法
WO2020132714A1 (en) * 2018-12-24 2020-07-02 Deakin University Method for amplification of nucleic acids
CN114891860A (zh) * 2022-03-31 2022-08-12 河南省人民医院 一种基于crispr和hrca技术的可视化靶dna检测方法
CN114921528A (zh) * 2022-04-22 2022-08-19 山东见微生物科技有限公司 一种核酸内切酶4介导的rna特异扩增方法
CN116004681B (zh) * 2022-08-15 2023-08-29 南京诺唯赞生物科技股份有限公司 一种提高topo克隆中载体连接效率的方法及试剂盒

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6977153B2 (en) * 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
WO2006090073A2 (fr) * 2005-02-25 2006-08-31 Comissariat A L'energie Atomique Procede et dispositif pour separer des cibles moleculaires dans un melange complexe
JP2008541705A (ja) * 2005-05-26 2008-11-27 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド 非標準塩基を含むプライマーを使用する等温鎖置換増幅
JP4918409B2 (ja) * 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
US8202972B2 (en) * 2007-01-10 2012-06-19 General Electric Company Isothermal DNA amplification
AU2010206492B2 (en) * 2009-01-21 2012-07-12 Human Genetic Signatures Pty Ltd Improved isothermal strand displacement amplification
JP2012080871A (ja) * 2009-12-14 2012-04-26 National Agriculture & Food Research Organization Ｒｎａの直接検出法
WO2014164479A1 (en) * 2013-03-11 2014-10-09 Elitech Holding B.V. Methods for true isothermal strand displacement amplification

Also Published As

Publication number Publication date
CA2877368A1 (en) 2014-01-03
EP2867366A2 (en) 2015-05-06
WO2014004852A3 (en) 2014-04-24
CN104583413B (zh) 2018-10-19
CN104583413A (zh) 2015-04-29
EP2867366A4 (en) 2016-04-20
JP2015526069A (ja) 2015-09-10
EP2867366B1 (en) 2018-05-16
WO2014004852A2 (en) 2014-01-03
CA2877368C (en) 2021-07-13

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