CN104583413B - 用于自rna模板开始的等温dna扩增试剂盒 - Google Patents

用于自rna模板开始的等温dna扩增试剂盒 Download PDF

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Publication number
CN104583413B
CN104583413B CN201380044858.1A CN201380044858A CN104583413B CN 104583413 B CN104583413 B CN 104583413B CN 201380044858 A CN201380044858 A CN 201380044858A CN 104583413 B CN104583413 B CN 104583413B
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dna
rna
template
primer
amplification
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Chinese (zh)
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CN104583413A (zh
Inventor
J.R.尼尔森
R.S.杜蒂
G.A.格罗斯曼
R.C.赫勒
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GE Healthcare UK Ltd
Global Life Sciences Solutions Operations UK Ltd
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General Electric Co
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Priority claimed from US13/538,955 external-priority patent/US9777319B2/en
Priority claimed from US13/538,962 external-priority patent/US20140004509A1/en
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Publication of CN104583413A publication Critical patent/CN104583413A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201380044858.1A 2012-06-29 2013-06-27 用于自rna模板开始的等温dna扩增试剂盒 Active CN104583413B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US13/538962 2012-06-29
US13/538,955 US9777319B2 (en) 2012-06-29 2012-06-29 Method for isothermal DNA amplification starting from an RNA template
US13/538,962 US20140004509A1 (en) 2012-06-29 2012-06-29 Kit for isothermal dna amplification starting from an rna template
US13/538955 2012-06-29
PCT/US2013/048229 WO2014004852A2 (en) 2012-06-29 2013-06-27 Kit for isothermal dna amplification starting from an rna template

Publications (2)

Publication Number Publication Date
CN104583413A CN104583413A (zh) 2015-04-29
CN104583413B true CN104583413B (zh) 2018-10-19

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CN201380044858.1A Active CN104583413B (zh) 2012-06-29 2013-06-27 用于自rna模板开始的等温dna扩增试剂盒

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EP (1) EP2867366B1 (enExample)
JP (1) JP6382189B2 (enExample)
CN (1) CN104583413B (enExample)
CA (1) CA2877368C (enExample)
WO (1) WO2014004852A2 (enExample)

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US20130323795A1 (en) 2007-01-10 2013-12-05 General Electric Company Endonuclase-assisted isothermal amplification using contamination-free reagents
CA2810856C (en) 2010-08-13 2022-05-17 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample
NZ723570A (en) 2012-04-09 2018-06-29 Envirologix Inc Compositions and methods for quantifying a nucleic acid sequence in a sample
WO2015022359A1 (en) * 2013-08-13 2015-02-19 General Electric Company Endonuclease-assisted isothermal amplification using contamination-free reagents
SI3108009T1 (sl) * 2014-02-18 2021-09-30 Illumina, Inc. Postopki in sestavki za profiliranje DNK
CN106460071B (zh) 2014-04-22 2021-09-10 一龙公司 增强和/或预测dna扩增的组合物和方法
BR112017008082A2 (pt) * 2014-10-20 2017-12-26 Envirologix Inc composições e métodos para detectar um vírus de rna
US9623415B2 (en) 2014-12-31 2017-04-18 Click Diagnostics, Inc. Devices and methods for molecular diagnostic testing
KR102378346B1 (ko) * 2016-05-17 2022-03-25 오리시로 제노믹스 가부시키가이샤 환상 dna의 증폭 방법
CN107400701A (zh) * 2016-05-18 2017-11-28 上海德沛生物科技有限公司 环状rna分子的扩增和测序方法
CN107541507A (zh) * 2016-06-29 2018-01-05 厦门大学 一种rna反转录扩增方法
CN109661273B (zh) * 2016-06-30 2022-11-04 维斯比医学公司 用于核酸提取的装置和方法
US10350307B2 (en) * 2017-09-18 2019-07-16 General Electric Company In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides
MX2020004783A (es) 2017-11-09 2020-08-13 Visby Medical Inc Dispositivo portatil para el diagnostico molecular y metodos para la deteccion de virus objetivo.
JP7029138B2 (ja) * 2018-03-05 2022-03-03 国立大学法人埼玉大学 連結核酸断片の製造方法、連結核酸断片、及び連結核酸断片から構成されるライブラリ
CN110819673B (zh) * 2018-08-07 2021-07-13 华中农业大学 一种原核生物cDNA5′末端快速扩增方法
WO2020132714A1 (en) * 2018-12-24 2020-07-02 Deakin University Method for amplification of nucleic acids
CA3214584A1 (en) * 2021-03-30 2022-10-06 Illumina, Inc. Improved methods of isothermal complementary dna and library preparation
CN114891860A (zh) * 2022-03-31 2022-08-12 河南省人民医院 一种基于crispr和hrca技术的可视化靶dna检测方法
CN114921528A (zh) * 2022-04-22 2022-08-19 山东见微生物科技有限公司 一种核酸内切酶4介导的rna特异扩增方法
CN116004681B (zh) * 2022-08-15 2023-08-29 南京诺唯赞生物科技股份有限公司 一种提高topo克隆中载体连接效率的方法及试剂盒

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102282258A (zh) * 2009-01-21 2011-12-14 人类遗传标记控股有限公司 改良的等温链置换扩增

Family Cites Families (7)

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Publication number Priority date Publication date Assignee Title
US6977153B2 (en) * 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
CA2598508A1 (fr) * 2005-02-25 2006-08-31 Commissariat A L'energie Atomique Procede et dispositif pour separer des cibles moleculaires dans un melange complexe
EP1883707B1 (en) * 2005-05-26 2014-04-09 Human Genetic Signatures PTY Ltd Isothermal strand displacement amplification using primers containing a non-regular base
JP4918409B2 (ja) * 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
US8202972B2 (en) * 2007-01-10 2012-06-19 General Electric Company Isothermal DNA amplification
JP2012080871A (ja) * 2009-12-14 2012-04-26 National Agriculture & Food Research Organization Ｒｎａの直接検出法
WO2014164479A1 (en) * 2013-03-11 2014-10-09 Elitech Holding B.V. Methods for true isothermal strand displacement amplification

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102282258A (zh) * 2009-01-21 2011-12-14 人类遗传标记控股有限公司 改良的等温链置换扩增

Also Published As

Publication number Publication date
WO2014004852A2 (en) 2014-01-03
CA2877368C (en) 2021-07-13
CA2877368A1 (en) 2014-01-03
CN104583413A (zh) 2015-04-29
WO2014004852A3 (en) 2014-04-24
JP2015526069A (ja) 2015-09-10
JP6382189B2 (ja) 2018-08-29
EP2867366B1 (en) 2018-05-16
EP2867366A4 (en) 2016-04-20
EP2867366A2 (en) 2015-05-06

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Patentee before: General Electric Co.

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