JP2018514230A5 - - Google Patents

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JP2018514230A5
JP2018514230A5 JP2018506808A JP2018506808A JP2018514230A5 JP 2018514230 A5 JP2018514230 A5 JP 2018514230A5 JP 2018506808 A JP2018506808 A JP 2018506808A JP 2018506808 A JP2018506808 A JP 2018506808A JP 2018514230 A5 JP2018514230 A5 JP 2018514230A5
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mainstream
primer
nucleotide
reverse
binding site
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JP6966681B2 (ja
JP2018514230A (ja
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JP2018506808A 2015-04-24 2016-04-22 限られたヌクレオチド組成を有するプライマーを用いた増幅 Active JP6966681B2 (ja)

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JP2021157314A JP2022008577A (ja) 2015-04-24 2021-09-28 限られたヌクレオチド組成を有するプライマーを用いた増幅

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US201562152756P 2015-04-24 2015-04-24
US62/152,756 2015-04-24
PCT/US2016/029054 WO2016172632A2 (en) 2015-04-24 2016-04-22 Amplification with primers of limited nucleotide composition

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JP2018514230A JP2018514230A (ja) 2018-06-07
JP2018514230A5 true JP2018514230A5 (enExample) 2019-05-23
JP6966681B2 JP6966681B2 (ja) 2021-11-17

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JP2021157314A Pending JP2022008577A (ja) 2015-04-24 2021-09-28 限られたヌクレオチド組成を有するプライマーを用いた増幅

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US (2) US11091799B2 (enExample)
EP (1) EP3286338B1 (enExample)
JP (2) JP6966681B2 (enExample)
CN (2) CN107849603B (enExample)
AU (2) AU2016252998B2 (enExample)
CA (1) CA2983819A1 (enExample)
ES (1) ES2961374T3 (enExample)
WO (1) WO2016172632A2 (enExample)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5960709B2 (ja) 2010-10-27 2016-08-02 プレジデント アンド フェローズ オブ ハーバード カレッジ トーホールドプライマーデュプレックスの組成物およびその使用方法
US11639522B2 (en) 2015-01-30 2023-05-02 President And Fellows Of Harvard College Microscope-free imaging
CN107849603B (zh) 2015-04-24 2022-01-28 阿提拉生物系统公司 利用有限核苷酸组成的引物扩增
EP3532635B1 (en) * 2016-10-31 2021-06-09 F. Hoffmann-La Roche AG Barcoded circular library construction for identification of chimeric products
IL267372B2 (en) * 2016-12-29 2024-02-01 Seegene Inc A method for reducing primer dimer formation and increasing amplification efficiency
JP7116062B2 (ja) * 2017-01-10 2022-08-09 プレジデント アンド フェローズ オブ ハーバード カレッジ 多重化シグナル増幅
CN106868111B (zh) * 2017-01-13 2020-12-25 中玉金标记(北京)生物技术股份有限公司 利用通用TaqMan探针检测SNP的方法和试剂盒
JP7026416B2 (ja) * 2017-04-17 2022-03-01 モンタナ・ステイト・ユニバーシティ 非線形増幅率を示すスイッチ様等温dna増幅
CA3072591A1 (en) * 2017-08-11 2019-02-14 Atila Biosystems Incorporated Digital amplification with primers of limited nucleotide composition
US20190112636A1 (en) * 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
CN111630179B (zh) 2018-01-26 2024-07-19 哈佛学院院长及董事 邻近检测方法和组合物
KR102180462B1 (ko) * 2018-01-31 2020-11-18 한국과학기술원 삼중접합구조 기반 등온 핵산 증폭 기술을 이용한 표적핵산 검출 방법
JP7013960B2 (ja) * 2018-03-13 2022-02-01 株式会社リコー 核酸検出方法及び核酸検出装置、並びに、核酸検出キット
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
DE102018213026A1 (de) * 2018-08-03 2020-02-06 Robert Bosch Gmbh Verfahren zur Durchführung einer Echtzeit-PCR
US20220090194A1 (en) * 2019-01-09 2022-03-24 3Cr Bioscience Ltd. Method for detecting a nucleic acid sequence
GB2587178B (en) * 2019-01-09 2021-10-06 3Cr Bioscience Ltd Method
US20220056498A1 (en) * 2019-01-25 2022-02-24 President And Fellows Of Harvard College Compositions and method for synthesizing nucleic acids
CN111593122A (zh) * 2019-02-20 2020-08-28 北京福安华生物科技有限公司 检测XPC基因rs2228001位点多态性的人工模拟核酸分子信标与试剂盒
CN111593105A (zh) * 2019-02-21 2020-08-28 葛猛 检测SOD2基因rs4880位点多态性的人工模拟核酸分子信标与试剂盒
CN110079584B (zh) * 2019-03-29 2020-07-28 中国农业大学 基于3d-hcr水凝胶的汞离子快速可视化检测方法
GB201910635D0 (en) * 2019-07-25 2019-09-11 Ttp Plc Detection of target oligonucleotides
CN112301103B (zh) * 2019-08-02 2023-04-11 北京贝瑞和康生物技术有限公司 一种非特异性扩增天然短片段核酸的方法和试剂盒
CN110542674B (zh) * 2019-09-19 2021-10-22 济南大学 一种检测谷胱甘肽的生物传感器及其制备方法
WO2021074087A1 (en) * 2019-10-16 2021-04-22 Illumina, Inc. Systems and methods for detecting multiple analytes
CA3185003A1 (en) * 2020-07-09 2022-01-13 Enos Kline Kits, methods, polypeptides, systems, and non-transitory, machine-readable storage media for detecting a nucleic acid
CN112899346A (zh) * 2021-01-05 2021-06-04 南京普济生物有限公司 一种降低pcr非特异性扩增的核苷酸及其设计方法与应用
CN112899357A (zh) * 2021-01-05 2021-06-04 南京普济生物有限公司 一种运用数字pcr进行胎儿分数检测的方法及其试剂盒
CN112899358A (zh) * 2021-01-06 2021-06-04 南京普济生物有限公司 一种无创产前胎儿染色体非整倍体的检测方法及其试剂盒
CN112735527B (zh) * 2021-01-06 2022-09-13 武汉华大基因技术服务有限公司 一种串联序列解析方法、装置和存储介质
CN112899365A (zh) * 2021-01-06 2021-06-04 南京普济生物有限公司 一种肺癌基因突变位点的检测方法及其试剂盒
CN112899366A (zh) * 2021-01-06 2021-06-04 南京普济生物有限公司 一种结直肠癌基因突变位点的检测方法及其试剂盒
CN114958988A (zh) * 2021-02-27 2022-08-30 上海境象生物科技有限公司 基于探针熔解曲线分析的多重核酸检测方法和试剂盒
WO2022216984A1 (en) * 2021-04-08 2022-10-13 Board Of Regents, The University Of Texas System Methods and systems for hpv detection and quantification
KR20220142832A (ko) * 2021-04-15 2022-10-24 프로지니어 주식회사 G-사중체화 서열 기반의 표적 핵산 검출용 핵산 템플릿 및 이의 용도
EP4409039A4 (en) * 2021-09-29 2025-09-03 Pillar Biosciences Inc CUSTOMIZED LIQUID BIOPSIES FOR CANCER USING PRIMERS FROM A PRIMER BANK
DE112022005006T5 (de) * 2021-10-20 2024-08-08 Tataa Biocenter Ab Verfahren und zusammensetzungen zur detektion mutierter nukleinsäuresequenzen
CN114196740A (zh) * 2021-12-09 2022-03-18 南京普济生物有限公司 用于同时识别多种基因类型的数字扩增检测方法、检测产品和检测试剂盒
CN116356075A (zh) * 2021-12-27 2023-06-30 迈克生物股份有限公司 一种用于检测的引物探针、引物探针组及其应用
US20250297318A1 (en) * 2024-03-25 2025-09-25 Milaboratories Inc. Dna-based adaptome profiling for minimal residual disease quantification in lymphoid malignancies
CN119023958B (zh) * 2024-10-28 2025-05-06 宁波大学 基于多价适配体和cha扩增的比色-荧光双信号检测沙门氏菌的方法

Family Cites Families (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
IE72468B1 (en) 1987-07-31 1997-04-09 Univ Leland Stanford Junior Selective amplification of target polynucleotide sequences
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
EP0731175A3 (en) 1989-07-11 2004-05-26 Gen-Probe Incorporated Oligonucleotide probes for HIV nucleic acid
DE69131891T2 (de) 1990-02-16 2000-06-15 F. Hoffmann-La Roche Ag, Basel Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion
DK0540693T3 (da) 1990-07-24 1999-09-13 Hoffmann La Roche Reduktion af ikke-specifik amplifikation under in vitro-nukleinsyreamplifikation under anvendelse af modificerede nukleinsy
IL100040A (en) 1990-11-13 1995-12-31 Siska Diagnostics Inc Nucleic acid amplification by two enzyme self-sustained sequence replication
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5470723A (en) 1993-05-05 1995-11-28 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
US5422252A (en) 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
SE506700C2 (sv) 1996-05-31 1998-02-02 Mikael Kubista Sond och förfaranden för analys av nukleinsyra
US6174670B1 (en) 1996-06-04 2001-01-16 University Of Utah Research Foundation Monitoring amplification of DNA during PCR
US6130038A (en) 1996-07-16 2000-10-10 Gen-Probe Incorporated Method for amplifying target nucleic acids using modified primers
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
DE69827060T2 (de) 1997-03-20 2005-03-24 F. Hoffmann-La Roche Ag Modifizierte Primer
US6794499B2 (en) 1997-09-12 2004-09-21 Exiqon A/S Oligonucleotide analogues
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
DE1020534T1 (de) 1998-11-09 2001-03-01 Eiken Kagaku K.K., Tokio/Tokyo Verfahren zur synthetisieren von nukleinsäure
EP1307592B1 (en) 2000-08-11 2006-07-05 University of Utah Research Foundation Single-labeled oligonucleotide probes
US7112423B2 (en) 2001-07-15 2006-09-26 Keck Graduate Institute Nucleic acid amplification using nicking agents
DE10236406C1 (de) * 2002-08-02 2003-12-24 Epigenomics Ag Verfahren zur Amplifikation von Nukleinsäuren mit geringer Komplexität
EP1539979B1 (en) 2002-09-20 2008-11-19 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
EP2374900B1 (en) * 2003-03-07 2016-07-13 Rubicon Genomics, Inc. Polynucleotides for the amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process
US7387876B2 (en) * 2004-02-27 2008-06-17 President And Fellows Of Harvard College Amplification of trace amounts of nucleic acids
ATE480550T1 (de) 2004-03-25 2010-09-15 California Inst Of Techn Hybridisierungskettenreaktion
US8871469B1 (en) 2004-11-13 2014-10-28 Steven Albert Benner Self-avoiding molecular recognition systems in DNA priming
ES2399054T3 (es) 2004-12-03 2013-03-25 Human Genetic Signatures Pty Ltd Métodos para simplificar ácidos nucleicos microbianos mediante modificación química de citosinas
EP2458011B1 (en) * 2005-10-03 2013-07-24 Life Technologies Corporation Compositions, methods, and kits for amplifying nucleic acids
US20090036315A1 (en) * 2006-02-07 2009-02-05 Antara Biosciences Inc. Device and methods for detecting and quantifying one or more target agents
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
KR101110013B1 (ko) * 2007-10-05 2012-02-29 (주)바이오니아 서열 내에 어베이직 부분을 포함하는 pcr 증폭용프라이머
KR20100019220A (ko) * 2008-08-08 2010-02-18 삼성전자주식회사 열순환을 포함하는 다중 치환 증폭에 의하여 표적 핵산 서열을 증폭하는 방법
US8658366B2 (en) 2008-09-18 2014-02-25 Roche Molecular Systems, Inc. Detection of target variants using a fluorescent label and a soluble quencher
FR2940805B1 (fr) * 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
EP3460074B1 (en) * 2010-02-05 2020-10-07 Quest Diagnostics Investments Incorporated Method to detect repeat sequence motifs in nucleic acid
US9074204B2 (en) 2011-05-20 2015-07-07 Fluidigm Corporation Nucleic acid encoding reactions
WO2014039092A1 (en) * 2012-09-06 2014-03-13 Life Technologies Corporation Multiplex y-str analysis
US20140274811A1 (en) * 2013-03-14 2014-09-18 Lyle J. Arnold Methods for Amplifying a Complete Genome or Transcriptome
ES2866044T3 (es) * 2014-02-18 2021-10-19 Illumina Inc Métodos y composiciones para la elaboración de perfiles de ADN
CN107849603B (zh) 2015-04-24 2022-01-28 阿提拉生物系统公司 利用有限核苷酸组成的引物扩增
CA3072591A1 (en) 2017-08-11 2019-02-14 Atila Biosystems Incorporated Digital amplification with primers of limited nucleotide composition

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