JP2012514473A5 - - Google Patents
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- Publication number
- JP2012514473A5 JP2012514473A5 JP2011545427A JP2011545427A JP2012514473A5 JP 2012514473 A5 JP2012514473 A5 JP 2012514473A5 JP 2011545427 A JP2011545427 A JP 2011545427A JP 2011545427 A JP2011545427 A JP 2011545427A JP 2012514473 A5 JP2012514473 A5 JP 2012514473A5
- Authority
- JP
- Japan
- Prior art keywords
- seq
- variable region
- chain variable
- light chain
- cdr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 108010047041 Complementarity Determining Regions Proteins 0.000 claims description 97
- 239000011541 reaction mixture Substances 0.000 claims description 28
- 230000004568 DNA-binding Effects 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 16
- 239000000654 additive Substances 0.000 claims description 12
- 230000000996 additive effect Effects 0.000 claims description 12
- FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 claims description 12
- 230000000694 effects Effects 0.000 claims description 11
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 9
- 230000003321 amplification Effects 0.000 claims description 8
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 8
- 108010010677 Phosphodiesterase I Proteins 0.000 claims description 7
- 108020004414 DNA Proteins 0.000 claims description 6
- 102000053602 DNA Human genes 0.000 claims description 6
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims description 6
- 108010077895 Sarcosine Proteins 0.000 claims description 6
- 229960002897 heparin Drugs 0.000 claims description 6
- 229920000669 heparin Polymers 0.000 claims description 6
- 108020004707 nucleic acids Proteins 0.000 claims description 6
- 102000039446 nucleic acids Human genes 0.000 claims description 6
- 150000007523 nucleic acids Chemical class 0.000 claims description 6
- 229940043230 sarcosine Drugs 0.000 claims description 6
- 108091034117 Oligonucleotide Proteins 0.000 claims description 4
- 238000011534 incubation Methods 0.000 claims description 4
- 230000027455 binding Effects 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 239000005547 deoxyribonucleotide Substances 0.000 claims description 2
- -1 deoxyribonucleotide triphosphates Chemical class 0.000 claims description 2
- 108091033319 polynucleotide Proteins 0.000 claims description 2
- 102000040430 polynucleotide Human genes 0.000 claims description 2
- 239000002157 polynucleotide Substances 0.000 claims description 2
- 229920001184 polypeptide Polymers 0.000 claims description 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 2
- 239000001226 triphosphate Substances 0.000 claims description 2
- 235000011178 triphosphate Nutrition 0.000 claims description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14335009P | 2009-01-08 | 2009-01-08 | |
| US61/143,350 | 2009-01-08 | ||
| PCT/US2010/020371 WO2010080910A1 (en) | 2009-01-08 | 2010-01-07 | Methods and compositions for improving efficiency of nucleic acids amplification reactions |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012514473A JP2012514473A (ja) | 2012-06-28 |
| JP2012514473A5 true JP2012514473A5 (enExample) | 2013-02-14 |
| JP5818688B2 JP5818688B2 (ja) | 2015-11-18 |
Family
ID=42316809
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011545427A Active JP5818688B2 (ja) | 2009-01-08 | 2010-01-07 | 核酸増幅反応の効率を改善するための方法および組成物 |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US8859205B2 (enExample) |
| EP (2) | EP2894226B1 (enExample) |
| JP (1) | JP5818688B2 (enExample) |
| CN (1) | CN102272319B (enExample) |
| CA (1) | CA2749093C (enExample) |
| SG (2) | SG10201500705YA (enExample) |
| WO (1) | WO2010080910A1 (enExample) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9181551B2 (en) | 2002-02-20 | 2015-11-10 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA) |
| US9657294B2 (en) | 2002-02-20 | 2017-05-23 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA) |
| CA2749093C (en) * | 2009-01-08 | 2019-03-26 | Bio-Rad Laboratories, Inc. | Exonuclease deficient hybrid polymerase and uses thereof in amplification reactions |
| EP2694670B1 (en) | 2011-04-08 | 2017-07-19 | Bio-Rad Laboratories, Inc. | Pcr reaction mixtures with decreased non-specific activity |
| US8697410B2 (en) | 2011-04-08 | 2014-04-15 | Bio-Rad Laboratories, Inc. | SSO7-polymerase conjugates with decreased non-specific activity |
| US8470573B2 (en) | 2011-06-21 | 2013-06-25 | Bio-Rad Laboratories, Inc. | Hybrid polymerases having the ability to produce long amplicons |
| CN103045554B (zh) * | 2012-11-29 | 2014-10-29 | 成都福际生物技术有限公司 | 一种制备热启动Taq酶的方法 |
| EP2971134B1 (en) * | 2013-03-15 | 2023-10-25 | Aegea Biotechnologies, Inc. | Methods for amplifying fragmented target nucleic acids utilizing an assembler sequence |
| CN105452451B (zh) | 2013-12-06 | 2020-06-05 | 生物辐射实验室股份有限公司 | 融合聚合酶 |
| US9963687B2 (en) | 2014-08-27 | 2018-05-08 | New England Biolabs, Inc. | Fusion polymerase and method for using the same |
| CN113584015B (zh) * | 2014-08-27 | 2025-05-02 | 新英格兰生物实验室公司 | 合成子的形成 |
| WO2017121843A1 (en) * | 2016-01-15 | 2017-07-20 | Thermo Fisher Scientific Baltics Uab | Antibodies that bind thermophilic dna polymerases |
| EP3402880B1 (en) | 2016-01-15 | 2024-01-03 | Thermo Fisher Scientific Baltics UAB | Thermophilic dna polymerase mutants |
| WO2019002178A1 (en) | 2017-06-26 | 2019-01-03 | Thermo Fisher Scientific Baltics Uab | DNA MUTANTS THERMOPHILIC POLYMERASES |
| EP3676400A4 (en) * | 2017-08-31 | 2021-06-02 | Seegene, Inc. | EVALUATING THE PERFORMANCE OF COMPONENTS USING A PAIR OF DIMERING PRIMERS |
| CN112375807B (zh) * | 2017-12-30 | 2023-02-21 | 浙江安诺优达生物科技有限公司 | 一种随机打断dna的方法 |
| WO2020264067A1 (en) | 2019-06-25 | 2020-12-30 | Bio-Rad Laboratories, Inc | Compositions and methods for enhancing reverse transcriptase activity and/or reducing the inhibition of reverse transcriptase |
| WO2021141970A1 (en) * | 2020-01-06 | 2021-07-15 | Pairwise Plants Services, Inc. | Recruitment of dna polymerase for templated editing |
| JP2021153393A (ja) * | 2020-03-25 | 2021-10-07 | 東洋紡株式会社 | 核酸増幅反応用組成物 |
| EP4471162A4 (en) * | 2022-01-26 | 2025-12-17 | Seegene Inc | METHOD FOR THE DETECTION OF TARGET NUCLEAN ACIDS IN A SAMPLE |
| WO2025116499A1 (en) * | 2023-11-27 | 2025-06-05 | Seegene, Inc. | Method for analyzing activity of nucleic acid polymerase using self-dimer primer |
Family Cites Families (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CU22545A1 (es) * | 1994-11-18 | 1999-03-31 | Centro Inmunologia Molecular | Obtención de un anticuerpo quimérico y humanizado contra el receptor del factor de crecimiento epidérmico para uso diagnóstico y terapéutico |
| US4545985A (en) | 1984-01-26 | 1985-10-08 | The United States Of America As Represented By The Secretary, Dept. Of Health And Human Services | Pseudomonas exotoxin conjugate immunotoxins |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4751180A (en) | 1985-03-28 | 1988-06-14 | Chiron Corporation | Expression using fused genes providing for protein product |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5679543A (en) | 1985-08-29 | 1997-10-21 | Genencor International, Inc. | DNA sequences, vectors and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi |
| US4935233A (en) | 1985-12-02 | 1990-06-19 | G. D. Searle And Company | Covalently linked polypeptide cell modulators |
| US6025545A (en) * | 1990-01-22 | 2000-02-15 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US5489523A (en) * | 1990-12-03 | 1996-02-06 | Stratagene | Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I |
| ATE188708T1 (de) * | 1991-08-21 | 2000-01-15 | Novartis Ag | Antikörperderivate |
| US5436149A (en) | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US5914110A (en) * | 1993-09-07 | 1999-06-22 | Smithkline Beecham Corporation | Recombinant IL4 antibodies useful in treatment of IL4 mediated disorders |
| JP3616394B2 (ja) * | 1994-01-31 | 2005-02-02 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | Ｄｎａ配列決定のアーティファクトの除去のための方法 |
| US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| US6015668A (en) | 1994-09-30 | 2000-01-18 | Life Technologies, Inc. | Cloned DNA polymerases from thermotoga and mutants thereof |
| US5912155A (en) | 1994-09-30 | 1999-06-15 | Life Technologies, Inc. | Cloned DNA polymerases from Thermotoga neapolitana |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| WO1997009451A1 (en) | 1995-09-08 | 1997-03-13 | Life Technologies, Inc. | Cloned dna polymerases from thermotoga and mutants thereof |
| AU726501B2 (en) * | 1996-06-04 | 2000-11-09 | University Of Utah Research Foundation | Monitoring hybridization during PCR |
| US6458556B1 (en) * | 1996-07-25 | 2002-10-01 | The Institute Of Physical & Chemical Research | Method for enhancing enzyme activity at elevated temperature |
| JP3206894B2 (ja) * | 1996-07-25 | 2001-09-10 | 理化学研究所 | 酵素を熱活性化する方法 |
| US6972323B1 (en) * | 1997-04-01 | 2005-12-06 | Sankyo Company, Limited | Anti-Fas antibodies |
| US5882904A (en) | 1997-08-04 | 1999-03-16 | Amersham Pharmacia Biotech Inc. | Thermococcus barossii DNA polymerase mutants |
| WO1999054455A1 (fr) * | 1998-04-23 | 1999-10-28 | Takara Shuzo Co., Ltd. | Procede de synthese de l'adn |
| US6673578B1 (en) * | 1998-09-08 | 2004-01-06 | Takara Shuzo Co., Ltd. | Method for synthesizing DNA |
| US20040031072A1 (en) * | 1999-05-06 | 2004-02-12 | La Rosa Thomas J. | Soy nucleic acid molecules and other molecules associated with transcription plants and uses thereof for plant improvement |
| JP3589912B2 (ja) * | 1999-09-03 | 2004-11-17 | 泰久 福井 | ホスファチジルイノシトール−３，４−二リン酸を認識するモノクローナル抗体 |
| IT1307309B1 (it) * | 1999-12-30 | 2001-10-30 | Enea Ente Nuove Tec | Peptidi stabilizzanti, polipeptidi ed anticorpi che li comprendono. |
| EP1266014A2 (en) * | 2000-03-24 | 2002-12-18 | Peter Kufer | Multifunctional polypeptides comprising a binding site to an epitope of the nkg2d receptor complex |
| US6627424B1 (en) | 2000-05-26 | 2003-09-30 | Mj Bioworks, Inc. | Nucleic acid modifying enzymes |
| US7408041B2 (en) * | 2000-12-08 | 2008-08-05 | Alexion Pharmaceuticals, Inc. | Polypeptides and antibodies derived from chronic lymphocytic leukemia cells and uses thereof |
| US20040131607A1 (en) * | 2001-06-08 | 2004-07-08 | Carroll Michael C. | Methods and compositions for inhibiting immunoglobulin-mediated reperfusion injury |
| EP1275735A1 (en) | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Composition and method for hot start nucleic acid amplification |
| US7365167B2 (en) * | 2001-11-26 | 2008-04-29 | Cell Matrix, Inc. | Humanized collagen antibodies and related methods |
| AU2002351198B2 (en) | 2001-11-28 | 2008-08-14 | Bio-Rad Laboratories, Inc. | Methods of using improved polymerases |
| AU2002351193A1 (en) | 2001-11-28 | 2003-06-10 | Mj Bioworks Incorporated | Parallel polymorphism scoring by amplification and error correction |
| US7560260B2 (en) | 2002-07-25 | 2009-07-14 | Bio-Rad Laboratories, Inc. | Compositions with polymerase activity |
| AU2003256794B2 (en) * | 2002-07-25 | 2008-07-31 | Bio-Rad Laboratories, Inc. | Hybrid protein methods and compositions |
| US7666645B2 (en) | 2002-10-23 | 2010-02-23 | Bio-Rad Laboratories, Inc. | Sso7-polymerase conjugate proteins |
| US8283148B2 (en) * | 2002-10-25 | 2012-10-09 | Agilent Technologies, Inc. | DNA polymerase compositions for quantitative PCR and methods thereof |
| AU2003301590A1 (en) * | 2002-10-25 | 2004-05-13 | Stratagene California | Dna polymerases with reduced base analog detection activity |
| EP2194123B1 (en) | 2003-03-25 | 2012-08-22 | Stratagene California | DNA polymerase fusions and uses thereof |
| US20040214194A1 (en) | 2003-04-17 | 2004-10-28 | Mj Bioworks, Inc. | Methods of making hybrid proteins |
| US20050025763A1 (en) * | 2003-05-08 | 2005-02-03 | Protein Design Laboratories, Inc. | Therapeutic use of anti-CS1 antibodies |
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| US20060005174A1 (en) | 2004-07-01 | 2006-01-05 | International Business Machines Corporation | Defining hierarchical structures with markup languages and reflection |
| US20060172324A1 (en) * | 2005-01-28 | 2006-08-03 | Roche Molecular Systems, Inc. | Methods of genotyping using differences in melting temperature |
| WO2007011891A2 (en) | 2005-07-15 | 2007-01-25 | Strategene California | Dna binding protein-polymerase chimeras |
| JP5376948B2 (ja) * | 2005-09-13 | 2013-12-25 | ナショナル リサーチ カウンシル オブ カナダ | 腫瘍細胞活性を調節する方法及び組成物 |
| WO2007076391A1 (en) * | 2005-12-23 | 2007-07-05 | Eli Lilly And Company | Tgf-beta binding compositions |
| JP5386367B2 (ja) * | 2006-12-29 | 2014-01-15 | アジレント・テクノロジーズ・インク | 分断ポリメラーゼを使用する組成物および方法 |
| JP2010518115A (ja) * | 2007-02-09 | 2010-05-27 | ジェネンテック， インコーポレイテッド | 抗Ｒｏｂｏ４抗体およびそれらのための使用 |
| EP2297343A1 (en) | 2008-05-16 | 2011-03-23 | New England Biolabs, Inc. | Enzyme reagents for amplification of polynucleotides in the presence of inhibitors |
| CA2749093C (en) * | 2009-01-08 | 2019-03-26 | Bio-Rad Laboratories, Inc. | Exonuclease deficient hybrid polymerase and uses thereof in amplification reactions |
-
2010
- 2010-01-07 CA CA2749093A patent/CA2749093C/en active Active
- 2010-01-07 SG SG10201500705YA patent/SG10201500705YA/en unknown
- 2010-01-07 WO PCT/US2010/020371 patent/WO2010080910A1/en not_active Ceased
- 2010-01-07 SG SG2011050093A patent/SG172925A1/en unknown
- 2010-01-07 US US12/683,950 patent/US8859205B2/en active Active
- 2010-01-07 JP JP2011545427A patent/JP5818688B2/ja active Active
- 2010-01-07 CN CN201080004057.9A patent/CN102272319B/zh active Active
- 2010-01-07 EP EP15155113.2A patent/EP2894226B1/en active Active
- 2010-01-07 EP EP10729512A patent/EP2382321A4/en not_active Withdrawn
-
2014
- 2014-09-10 US US14/482,756 patent/US9994887B2/en active Active
-
2018
- 2018-05-11 US US15/977,254 patent/US10787694B2/en active Active
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