JP2014511692A5 - - Google Patents
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- Publication number
- JP2014511692A5 JP2014511692A5 JP2014503656A JP2014503656A JP2014511692A5 JP 2014511692 A5 JP2014511692 A5 JP 2014511692A5 JP 2014503656 A JP2014503656 A JP 2014503656A JP 2014503656 A JP2014503656 A JP 2014503656A JP 2014511692 A5 JP2014511692 A5 JP 2014511692A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- polymerase
- seq
- free arginine
- sufficient
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 21
- 108020004707 nucleic acids Proteins 0.000 claims 13
- 150000007523 nucleic acids Chemical class 0.000 claims 13
- 102000039446 nucleic acids Human genes 0.000 claims 13
- 239000000203 mixture Substances 0.000 claims 7
- 239000004475 Arginine Substances 0.000 claims 6
- 230000003321 amplification Effects 0.000 claims 6
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 6
- 238000003199 nucleic acid amplification method Methods 0.000 claims 6
- 230000004568 DNA-binding Effects 0.000 claims 4
- 150000001413 amino acids Chemical group 0.000 claims 4
- 239000000872 buffer Substances 0.000 claims 4
- 239000003795 chemical substances by application Substances 0.000 claims 4
- 150000003839 salts Chemical class 0.000 claims 4
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 claims 4
- PFNFFQXMRSDOHW-UHFFFAOYSA-N spermine Chemical compound NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 claims 4
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 claims 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical group OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims 2
- AJTVSSFTXWNIRG-UHFFFAOYSA-N 2-[bis(2-hydroxyethyl)amino]ethanesulfonic acid Chemical compound OCC[NH+](CCO)CCS([O-])(=O)=O AJTVSSFTXWNIRG-UHFFFAOYSA-N 0.000 claims 2
- ACERFIHBIWMFOR-UHFFFAOYSA-N 2-hydroxy-3-[(1-hydroxy-2-methylpropan-2-yl)azaniumyl]propane-1-sulfonate Chemical compound OCC(C)(C)NCC(O)CS(O)(=O)=O ACERFIHBIWMFOR-UHFFFAOYSA-N 0.000 claims 2
- LVQFQZZGTZFUNF-UHFFFAOYSA-N 2-hydroxy-3-[4-(2-hydroxy-3-sulfonatopropyl)piperazine-1,4-diium-1-yl]propane-1-sulfonate Chemical compound OS(=O)(=O)CC(O)CN1CCN(CC(O)CS(O)(=O)=O)CC1 LVQFQZZGTZFUNF-UHFFFAOYSA-N 0.000 claims 2
- DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 claims 2
- NUFBIAUZAMHTSP-UHFFFAOYSA-N 3-(n-morpholino)-2-hydroxypropanesulfonic acid Chemical compound OS(=O)(=O)CC(O)CN1CCOCC1 NUFBIAUZAMHTSP-UHFFFAOYSA-N 0.000 claims 2
- RZQXOGQSPBYUKH-UHFFFAOYSA-N 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound OCC(CO)(CO)NCC(O)CS(O)(=O)=O RZQXOGQSPBYUKH-UHFFFAOYSA-N 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
- 239000007991 ACES buffer Substances 0.000 claims 2
- 108700016232 Arg(2)-Sar(4)- dermorphin (1-4) Proteins 0.000 claims 2
- 239000003155 DNA primer Substances 0.000 claims 2
- 239000007995 HEPES buffer Substances 0.000 claims 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims 2
- 239000007993 MOPS buffer Substances 0.000 claims 2
- FSVCELGFZIQNCK-UHFFFAOYSA-N N,N-bis(2-hydroxyethyl)glycine Chemical compound OCCN(CCO)CC(O)=O FSVCELGFZIQNCK-UHFFFAOYSA-N 0.000 claims 2
- DBXNUXBLKRLWFA-UHFFFAOYSA-N N-(2-acetamido)-2-aminoethanesulfonic acid Chemical compound NC(=O)CNCCS(O)(=O)=O DBXNUXBLKRLWFA-UHFFFAOYSA-N 0.000 claims 2
- JOCBASBOOFNAJA-UHFFFAOYSA-N N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid Chemical compound OCC(CO)(CO)NCCS(O)(=O)=O JOCBASBOOFNAJA-UHFFFAOYSA-N 0.000 claims 2
- 239000007990 PIPES buffer Substances 0.000 claims 2
- 108010010677 Phosphodiesterase I Proteins 0.000 claims 2
- 208000034953 Twin anemia-polycythemia sequence Diseases 0.000 claims 2
- 229960003121 arginine Drugs 0.000 claims 2
- 150000001484 arginines Chemical class 0.000 claims 2
- 239000007998 bicine buffer Substances 0.000 claims 2
- 229940063673 spermidine Drugs 0.000 claims 2
- 229940063675 spermine Drugs 0.000 claims 2
- 239000011541 reaction mixture Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161473710P | 2011-04-08 | 2011-04-08 | |
| US61/473,710 | 2011-04-08 | ||
| PCT/US2012/024735 WO2012138416A1 (en) | 2011-04-08 | 2012-02-10 | Pcr reaction mixtures with decreased non-specific activity |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016192414A Division JP2017029161A (ja) | 2011-04-08 | 2016-09-30 | 非特異的活性が低下したｐｃｒ反応混合物 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014511692A JP2014511692A (ja) | 2014-05-19 |
| JP2014511692A5 true JP2014511692A5 (enExample) | 2014-11-13 |
| JP6072766B2 JP6072766B2 (ja) | 2017-02-01 |
Family
ID=46966404
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014503656A Active JP6072766B2 (ja) | 2011-04-08 | 2012-02-10 | 非特異的活性が低下したｐｃｒ反応混合物 |
| JP2016192414A Withdrawn JP2017029161A (ja) | 2011-04-08 | 2016-09-30 | 非特異的活性が低下したｐｃｒ反応混合物 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016192414A Withdrawn JP2017029161A (ja) | 2011-04-08 | 2016-09-30 | 非特異的活性が低下したｐｃｒ反応混合物 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US8916352B2 (enExample) |
| EP (1) | EP2694670B1 (enExample) |
| JP (2) | JP6072766B2 (enExample) |
| CN (1) | CN103703141B (enExample) |
| SG (1) | SG193990A1 (enExample) |
| WO (1) | WO2012138416A1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9493824B2 (en) * | 2010-12-16 | 2016-11-15 | Bio-Rad Laboratories, Inc. | Universal reference dye for quantitative amplification |
| US10131898B2 (en) | 2014-07-22 | 2018-11-20 | Bio-Rad Laboratories, Inc. | Buffers for use with polymerases |
| EP3387002A4 (en) | 2015-12-07 | 2019-04-24 | Bio-Rad Laboratories, Inc. | DIM REVERSE TRANSCRIPTASE |
| WO2017121843A1 (en) | 2016-01-15 | 2017-07-20 | Thermo Fisher Scientific Baltics Uab | Antibodies that bind thermophilic dna polymerases |
| EP3402880B1 (en) | 2016-01-15 | 2024-01-03 | Thermo Fisher Scientific Baltics UAB | Thermophilic dna polymerase mutants |
| GB201704401D0 (en) * | 2017-03-20 | 2017-05-03 | Epistem Ltd | Use of anticoagulants |
| CN110945141B (zh) * | 2017-06-22 | 2023-08-08 | 生命技术公司 | 基于甲磺酸盐的预混液 |
| WO2019002178A1 (en) | 2017-06-26 | 2019-01-03 | Thermo Fisher Scientific Baltics Uab | DNA MUTANTS THERMOPHILIC POLYMERASES |
| WO2019036682A1 (en) | 2017-08-18 | 2019-02-21 | Modernatx, Inc. | RNA VARIANTS POLYMERASE |
| JP2022521094A (ja) | 2019-02-20 | 2022-04-05 | モデルナティエックス インコーポレイテッド | 共転写キャッピング用ｒｎａポリメラーゼバリアント |
| CN112176042B (zh) * | 2019-07-03 | 2023-10-17 | 赛纳生物科技(北京)有限公司 | 一种基因测序试剂及方法 |
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| US4246194A (en) | 1977-12-08 | 1981-01-20 | Research Organics, Inc. | Amino-hydroxy-alkyl sulfonic acid-zwitterions |
| US4545985A (en) | 1984-01-26 | 1985-10-08 | The United States Of America As Represented By The Secretary, Dept. Of Health And Human Services | Pseudomonas exotoxin conjugate immunotoxins |
| US4751180A (en) | 1985-03-28 | 1988-06-14 | Chiron Corporation | Expression using fused genes providing for protein product |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US5679543A (en) | 1985-08-29 | 1997-10-21 | Genencor International, Inc. | DNA sequences, vectors and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi |
| US4935233A (en) | 1985-12-02 | 1990-06-19 | G. D. Searle And Company | Covalently linked polypeptide cell modulators |
| US5489523A (en) | 1990-12-03 | 1996-02-06 | Stratagene | Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I |
| DE4111393A1 (de) | 1991-04-09 | 1992-10-15 | Behringwerke Ag | Stabilisierte faktor viii-praeparationen |
| US5962292A (en) | 1993-09-07 | 1999-10-05 | Kyowa Hakko Kogyo Co., Ltd. | Process for producing cis-3-hydroxy-L-proline |
| US6063566A (en) | 1994-05-13 | 2000-05-16 | The Scripps Research Institute | Catalytic RNA molecules |
| US6015668A (en) | 1994-09-30 | 2000-01-18 | Life Technologies, Inc. | Cloned DNA polymerases from thermotoga and mutants thereof |
| US5912155A (en) | 1994-09-30 | 1999-06-15 | Life Technologies, Inc. | Cloned DNA polymerases from Thermotoga neapolitana |
| US6413747B1 (en) * | 1994-10-03 | 2002-07-02 | Shimadzu Corporation | Enhancement of nucleic acid amplification by the addition of a polyamine |
| WO1997009451A1 (en) | 1995-09-08 | 1997-03-13 | Life Technologies, Inc. | Cloned dna polymerases from thermotoga and mutants thereof |
| US6037128A (en) * | 1997-03-31 | 2000-03-14 | Council Of Scientific & Industrial Research | Process for the preparation of semisynthetic amplicon useful for sex determination of the papaya plant |
| US5882904A (en) | 1997-08-04 | 1999-03-16 | Amersham Pharmacia Biotech Inc. | Thermococcus barossii DNA polymerase mutants |
| ES2435141T3 (es) | 1999-02-22 | 2013-12-18 | University Of Connecticut | Nuevas formulaciones de factor VIII libres de albúmina |
| US6951722B2 (en) * | 1999-03-19 | 2005-10-04 | Takara Bio Inc. | Method for amplifying nucleic acid sequence |
| US6627424B1 (en) | 2000-05-26 | 2003-09-30 | Mj Bioworks, Inc. | Nucleic acid modifying enzymes |
| WO2002062993A1 (en) * | 2001-02-06 | 2002-08-15 | Takara Bio Inc. | Amplified nucleic acids and immobilized products thereof |
| AU2002351193A1 (en) | 2001-11-28 | 2003-06-10 | Mj Bioworks Incorporated | Parallel polymorphism scoring by amplification and error correction |
| AU2002351198B2 (en) | 2001-11-28 | 2008-08-14 | Bio-Rad Laboratories, Inc. | Methods of using improved polymerases |
| US7560260B2 (en) * | 2002-07-25 | 2009-07-14 | Bio-Rad Laboratories, Inc. | Compositions with polymerase activity |
| AU2003256794B2 (en) * | 2002-07-25 | 2008-07-31 | Bio-Rad Laboratories, Inc. | Hybrid protein methods and compositions |
| US7666645B2 (en) * | 2002-10-23 | 2010-02-23 | Bio-Rad Laboratories, Inc. | Sso7-polymerase conjugate proteins |
| US8283148B2 (en) | 2002-10-25 | 2012-10-09 | Agilent Technologies, Inc. | DNA polymerase compositions for quantitative PCR and methods thereof |
| EP2194123B1 (en) * | 2003-03-25 | 2012-08-22 | Stratagene California | DNA polymerase fusions and uses thereof |
| EP1608735A4 (en) * | 2003-04-03 | 2008-11-05 | Alnylam Pharmaceuticals | RNAI CONJUGATES |
| US20040214194A1 (en) | 2003-04-17 | 2004-10-28 | Mj Bioworks, Inc. | Methods of making hybrid proteins |
| US20070117094A1 (en) * | 2003-09-30 | 2007-05-24 | Yoshihide Hayashizaki | Support, printed material and reagent kit having enzyme fixed thereon, method for preparing the support, method of storing enzyme and method for restoration enzymes |
| ATE452992T1 (de) | 2004-02-04 | 2010-01-15 | Qiagen North American Holdings | Zusammensetzungen auf dutp-basis zurverringerung der primeraggregatbildung während der nukleinsäureamplifikation |
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| AU2007275365A1 (en) * | 2006-07-17 | 2008-01-24 | Sirna Therapeutics Inc. | RNA interference mediated inhibition of Proprotein Convertase Subtilisin Kexin 9 (PCSK9) gene expression using short interfering nucleic acid (siNA) |
| HUE037173T2 (hu) * | 2006-08-08 | 2018-08-28 | Univ Bonn Rheinische Friedrich Wilhelms | 5'-Foszfát-oligonukleotidok szerkezete és alkalmazása |
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| AR066984A1 (es) * | 2007-06-15 | 2009-09-23 | Novartis Ag | Inhibicion de la expresion de la subunidad alfa del canal epitelial de sodio (enac) por medio de arni (arn de interferencia) |
| CA2964559C (en) | 2007-11-27 | 2020-01-14 | Bio-Rad Laboratories, Inc. | Reduced inhibition of one-step rt-pcr |
| CA2749093C (en) * | 2009-01-08 | 2019-03-26 | Bio-Rad Laboratories, Inc. | Exonuclease deficient hybrid polymerase and uses thereof in amplification reactions |
| AU2010258715B2 (en) * | 2009-06-12 | 2015-04-09 | Revvity Health Sciences, Inc. | Rehydratable matrices for dry storage of TAQ polymerase in a microfluidic device |
| WO2012083175A1 (en) | 2010-12-17 | 2012-06-21 | Wisconsin Alumni Research Foundation | One-step method of elution of dna from blood samples |
-
2012
- 2012-02-10 EP EP12767971.0A patent/EP2694670B1/en active Active
- 2012-02-10 WO PCT/US2012/024735 patent/WO2012138416A1/en not_active Ceased
- 2012-02-10 JP JP2014503656A patent/JP6072766B2/ja active Active
- 2012-02-10 CN CN201280026623.5A patent/CN103703141B/zh active Active
- 2012-02-10 US US13/370,604 patent/US8916352B2/en active Active
- 2012-02-10 SG SG2013072830A patent/SG193990A1/en unknown
-
2014
- 2014-10-13 US US14/512,924 patent/US9556423B2/en active Active
-
2016
- 2016-09-30 JP JP2016192414A patent/JP2017029161A/ja not_active Withdrawn
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